Carbon dots fluorescence can be used to distinguish isobaric peptide and to monitor protein oligomerization dynamics.
Methods
; 230: 1-8, 2024 Oct.
Article
en En
| MEDLINE
| ID: mdl-39038505
ABSTRACT
Carbon dots (CD) are widely investigated particles with interesting fluorescent properties which are reported to be used for various purposes, as they are biocompatible, resistant to photobleaching and with tuneable properties depending on the specific CD surface chemistry. In this work, we report on the possibility to use opportunely designed CD to distinguish among isobaric peptides almost undistinguishable by mass spectrometry, as well as to monitor protein aggregation phenomena. Particularly, cell-penetrating peptides containing the carnosine moiety at different positions in the peptide chain produce sequence specific fluorescent signals. Analogously, different insulin oligomerization states can also be distinguished by the newly proposed experimental approach. The latter is here described in details and can be potentially applied to any kind of peptide or protein.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Carbono
Límite:
Humans
Idioma:
En
Revista:
Methods
Asunto de la revista:
BIOQUIMICA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Italia