Pitfalls in the somatomedin-C radioimmunoassay.
J Clin Endocrinol Metab
; 54(5): 1084-6, 1982 May.
Article
en En
| MEDLINE
| ID: mdl-7199537
ABSTRACT
We have investigated the effects of various modifications on the quantitation of Somatomedin-C (Sm-C) by the Sm-C radio immunoassay. We have confirmed that the assay quantitatively measures Sm-C in serum at plasma samples when borosilicate tubes and protamine free buffers are used. Polystyrene tubes and/or protamine containing buffers interfere with the quantitative measurement of the hormone, apparently by modifying the binding of Sm-C to its binding protein. The implication of these modifications on the clinical use of the Sm-C RIA are discussed.
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Banco de datos:
MEDLINE
Asunto principal:
Somatomedinas
Límite:
Humans
Idioma:
En
Revista:
J Clin Endocrinol Metab
Año:
1982
Tipo del documento:
Article