Promoters containing ATF-binding sites are de-regulated in cells that express the EWS/ATF1 oncogene.
Oncogene
; 10(9): 1749-56, 1995 May 04.
Article
en En
| MEDLINE
| ID: mdl-7753552
ABSTRACT
Chromosomal translocations that fuse the N-terminal region of the Ewings sarcoma oncogene (EWS) to the C-terminal region (including the DNA-binding domain) of the cellular transcription factor ATF1 are associated with a tumour type termed malignant melanoma of soft parts (MMSP). It is envisioned that transformation by the EWS/ATF1 fusion protein results from aberrant transcriptional regulation of genes that are normally regulated by ATF1. To examine this hypothesis we have expressed exogenous EWS-ATF1 in JEG3 cells and tested its ability to activate several promoters that contain binding sites for ATF1. We show that EWS-ATF1 is a strong constitutive activator of some promoters tested but represses others. Significantly, the ability of particular promoters to be activated by EWS/ATF1 in JEG3 cells correlates with promoter activity in two MMSP-derived cell lines (SU-CCS-1 and DTC1). Our results therefore provide evidence that endogenous EWS/ATF1 can de-regulate transcription and that this capacity may contribute to transformation in MMSP.
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Banco de datos:
MEDLINE
Asunto principal:
Ribonucleoproteínas
/
Factores de Transcripción
/
Proteínas Nucleares
/
Regulación de la Expresión Génica
/
Regiones Promotoras Genéticas
/
Proteínas de Unión al ARN
/
Melanoma
Límite:
Humans
Idioma:
En
Revista:
Oncogene
Asunto de la revista:
BIOLOGIA MOLECULAR
/
NEOPLASIAS
Año:
1995
Tipo del documento:
Article
País de afiliación:
Reino Unido