Defects of insulin and IGF-1 action at receptor and postreceptor level in a patient with type A syndrome of insulin resistance.
Biochem Biophys Res Commun
; 234(3): 626-30, 1997 May 29.
Article
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| MEDLINE
| ID: mdl-9175764
ABSTRACT
The action of insulin and IGF-1 in comparison to non-diabetic controls was studied in cultured fibroblasts of a patient with an inherited syndrome of insulin resistance (Type A syndrome). Insulin binding was reduced due to decreased receptor affinity, but sequence analyses revealed no alterations of splicing or primary insulin receptor (IR) structure. Most likely due to the IR affinity defect analyses of signal transduction pathways showed an impairment of insulin action on glucose uptake, total RNA synthesis and phosphorylation as well as activity of MAP-kinase. In addition inducibility of c-fos mRNA level was strongly impaired by insulin and IGF-1, but comparable to controls by PDGF indicating a postreceptor defect. In conclusion, we provide evidence that genetic syndromes of insulin resistance can be associated with both, receptor and postreceptor defects.
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Banco de datos:
MEDLINE
Asunto principal:
Resistencia a la Insulina
/
Factor I del Crecimiento Similar a la Insulina
/
Receptor de Insulina
/
Receptor IGF Tipo 1
/
Insulina
Límite:
Female
/
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1997
Tipo del documento:
Article
País de afiliación:
Alemania