ABSTRACT
OBJECTIVE@#To explore the relationship between degradation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in the mouse liver and postmortem interval (PMI).@*METHODS@#Sixty NIH mice were sacrificed by cervical dislocation and suffocation, and then placed into 10 degrees C and 25 degrees C temperature-controlling systems. The changes of GAPDH mRNA in the liver were detected by two-step fluorimetric reverse transcriptase polymerase chain reaction (RT-PCR) technique and nucleic acids protein cryoscope from 0 to 48 h postmortem.@*RESULTS@#In the mouse liver, the amplification products of GAPDH mRNA could be examined within 48 h postmortem in 10 degrees C temperature-controlling system and within 36 h postmortem in 25 degrees C temperature-controlling system. The amplification products showed a decreasing tendency.@*CONCLUSION@#Degradation of GAPDH mRNA in the mouse liver is negative correlation with PMI. GAPDH mRNA could be a new marker for estimation of PMI.
Subject(s)
Animals , Female , Male , Mice , Forensic Pathology , Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism , Liver/metabolism , Mice, Inbred Strains , Postmortem Changes , RNA Stability , RNA, Messenger/metabolism , Regression Analysis , Reverse Transcriptase Polymerase Chain Reaction , Temperature , Time FactorsABSTRACT
OBJECTIVE@#To research the value of polymorphism of salivary esterase(Set) in paternity and personal identification.@*METHODS@#Phenotype and genotype of human salivary esterase were detected in 114 liquid saliva samples from the Chinese population by disc electrophoresis and fast blue RR staining assay.@*RESULTS@#The frequency of Set type was F 22.81%, FS 50.88%, S2 6.31%. The estimated gene frequency of SetF was 0.4825 and SetS was 0.5175. The PE was 0.1875 and the DP was 0.6199.@*CONCLUSION@#Polymorphism of salivary esterase (Set) was practical in paternity and personal identification.