ABSTRACT
The Mitochondrial Carrier Family (MCF) is a signature group of integral membrane proteins that transport metabolites across the mitochondrial inner membrane in eukaryotes. MCF proteins are characterized by six transmembrane segments that assemble to form a highly-selective channel for metabolite transport. We discovered a novel MCF member, termed Legionellanucleotide carrier Protein (LncP), encoded in the genome of Legionella pneumophila, the causative agent of Legionnaire's disease. LncP was secreted via the bacterial Dot/Icm type IV secretion system into macrophages and assembled in the mitochondrial inner membrane. In a yeast cellular system, LncP induced a dominant-negative phenotype that was rescued by deleting an endogenous ATP carrier. Substrate transport studies on purified LncP reconstituted in liposomes revealed that it catalyzes unidirectional transport and exchange of ATP transport across membranes, thereby supporting a role for LncP as an ATP transporter. A hidden Markov model revealed further MCF proteins in the intracellular pathogens, Legionella longbeachae and Neorickettsia sennetsu, thereby challenging the notion that MCF proteins exist exclusively in eukaryotic organisms.
Subject(s)
Bacterial Proteins/metabolism , Bacterial Secretion Systems/physiology , Carrier Proteins/metabolism , Legionella pneumophila/metabolism , Legionnaires' Disease/metabolism , Membrane Proteins/metabolism , Adenosine Triphosphate , Bacterial Proteins/genetics , Carrier Proteins/genetics , Genetic Complementation Test , HeLa Cells , Humans , Legionella pneumophila/genetics , Legionella pneumophila/pathogenicity , Legionnaires' Disease/genetics , Membrane Proteins/genetics , Neorickettsia sennetsu/genetics , Neorickettsia sennetsu/metabolism , Neorickettsia sennetsu/pathogenicity , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolismABSTRACT
Recent work has shown that a domain of YopE of Yersinia pseudotuberculosis ranging from amino acids 54 to 75 (R. Krall, Y. Zhang, and J. T. Barbieri, J. Biol. Chem. 279:2747-2753, 2004) is required for proper localization of YopE after ectopic expression in eukaryotic cells. This domain, called the membrane localization domain (MLD), has not been extensively studied in Yersinia. Therefore, an in cis MLD deletion mutant of YopE was created in Y. pseudotuberculosis. The mutant was found to secrete and translocate YopE at wild-type levels. However, the mutant was defective in the autoregulation of YopE expression after the infection of HeLa cells. Although the mutant translocated YopE at wild-type levels, it showed a delayed HeLa cell cytotoxicity. This delay was not caused by a change in GTPase activating protein (GAP) activity, since the mutant showed wild-type YopE GAP activity toward Rac1 and RhoA. The MLD mutant displayed a changed intracellular localization pattern of YopE in HeLa cells after infection, and the YopEDeltaMLD protein was found to be dispersed within the whole cell, including the nucleus. In contrast, wild-type YopE was found to localize to the perinuclear region of the cell and was not found in the nucleus. In addition, the yopEDeltaMLD mutant was avirulent. Our results suggest that YopE must target proteins other than RhoA and Rac1 and that the MLD is required for the proper targeting and hence virulence of YopE during infection. Our results raise the question whether YopE is a regulatory protein or a "true" virulence effector protein.
Subject(s)
Bacterial Outer Membrane Proteins/metabolism , Yersinia pseudotuberculosis/metabolism , Yersinia pseudotuberculosis/pathogenicity , Amino Acid Sequence , Bacterial Outer Membrane Proteins/genetics , Fluorescent Antibody Technique , HeLa Cells , Humans , Molecular Sequence Data , Protein Transport/physiology , Yersinia pseudotuberculosis/genetics , Yersinia pseudotuberculosis Infections/geneticsABSTRACT
The bacterial pathogen Yersinia pseudotuberculosis uses a type III secretion (T3S) system to translocate Yop effectors into eukaryotic cells. Effectors are thought to gain access to the cytosol via pores formed in the host cell plasma membrane. Translocated YopE can modulate this pore formation through its GTPase-activating protein (GAP) activity. In this study, we analysed the role of translocated YopE and all the other known Yop effectors in the regulation of effector translocation. Elevated levels of Yop effector translocation into HeLa cells occurred by YopE-defective strains, but not those defective for other Yop effectors. Only Yersinia devoid of YopK exhibits a similar hyper-translocation phenotype. Since both yopK and yopE mutants also failed to down-regulate Yop synthesis in the presence of eukaryotic cells, these data imply that translocated YopE specifically regulates subsequent effector translocation by Yersinia through at least one mechanism that involves YopK. We suggest that the GAP activity of YopE might be working as an intra-cellular probe measuring the amount of protein translocated by Yersinia during infection. This may be a general feature of T3S-associated GAP proteins, since two homologues from Pseudomonas aeruginosa, exoenzyme S (ExoS) and exoenzyme T (ExoT), can complement the hyper-translocation phenotypes of the yopE GAP mutant.
Subject(s)
Bacterial Outer Membrane Proteins/metabolism , Protein Transport/physiology , Yersinia pseudotuberculosis/metabolism , ADP Ribose Transferases/metabolism , Bacterial Outer Membrane Proteins/pharmacology , Bacterial Proteins/metabolism , Bacterial Proteins/pharmacology , Bacterial Toxins/pharmacology , Feedback, Physiological , GTPase-Activating Proteins/metabolism , HeLa Cells , Humans , L-Lactate Dehydrogenase/metabolism , Mutation , Virulence , Yersinia pseudotuberculosis/genetics , Yersinia pseudotuberculosis/pathogenicity , Yersinia pseudotuberculosis Infections/physiopathologyABSTRACT
Pathogenic Yersinia sp. utilise a common type III secretion system to translocate several anti-host Yop effectors into the cytosol of target eukaryotic cells. The secreted YopB and YopD translocator proteins are essential for this process, forming pores in biological membranes through which the effectors are thought to gain access to the cell interior. The non-secreted cognate chaperone, LcrH, also plays an important role by ensuring pre-secretory stabilisation and efficient secretion of YopB and YopD. This suggests that LcrH-regulated secretion of the translocators could be used by Yersinia to control effector translocation levels. We collected several LcrH mutants impaired in chaperone activity. These poorly bound, stabilised and/or secreted YopB and YopD in vitro. However, these mutants generally maintained stable substrates during a HeLa cell infection and these infected cells were intoxicated by translocated effectors. Surprisingly, this occurred in the absence of detectable YopB- and YopD-dependent pores in eukaryotic membranes. A functional type III translocon must therefore only require minuscule amounts of secreted translocator proteins. Based on these observations, LcrH dependent control of translocation via regulated YopB and YopD secretion would need to be exquisitely tight.
Subject(s)
Bacterial Outer Membrane Proteins/biosynthesis , Bacterial Proteins/physiology , Molecular Chaperones/physiology , Virulence Factors/metabolism , Yersinia pseudotuberculosis/pathogenicity , Bacterial Proteins/biosynthesis , Bacterial Proteins/genetics , HeLa Cells , Humans , Molecular Chaperones/genetics , Mutagenesis , Point Mutation , Pore Forming Cytotoxic Proteins/biosynthesis , Protein Binding , Protein Transport/genetics , Yersinia pseudotuberculosis/genetics , Yersinia pseudotuberculosis/metabolismABSTRACT
Body length in C. elegans is regulated by a member of the TGFbeta family, DBL-1. Loss-of-function mutations in dbl-1, or in genes encoding components of the signaling pathway it activates, cause worms to be shorter than wild type and slightly thinner (Sma). Overexpression of dbl-1 confers the Lon phenotype characterized by an increase in body length. We show here that loss-of-function mutations in dbl-1 and lon-1, respectively, cause a decrease or increase in the ploidy of nuclei in the hypodermal syncytial cell, hyp7. To learn more about the regulation of body length in C. elegans we carried out a genetic screen for new mutations causing a Lon phenotype. We report here the cloning and characterization of lon-3. lon-3 is shown to encode a putative cuticle collagen that is expressed in hypodermal cells. We show that, whereas putative null mutations in lon-3 (or reduction of lon-3 activity by RNAi) causes a Lon phenotype, increasing lon-3 gene copy number causes a marked reduction in body length. Morphometric analyses indicate that the lon-3 loss-of-function phenotype resembles that caused by overexpression of dbl-1. Furthermore, phenotypes caused by defects in dbl-1 or lon-3 expression are in both cases suppressed by a null mutation in sqt-1, a second cuticle collagen gene. However, whereas loss of dbl-1 activity causes a reduction in hypodermal endoreduplication, the reduction in body length associated with overexpression of lon-3 occurs in the absence of defects in hypodermal ploidy.
Subject(s)
Caenorhabditis elegans Proteins/genetics , Caenorhabditis elegans/genetics , Collagen/genetics , Transforming Growth Factor beta , Amino Acid Sequence , Animals , Caenorhabditis elegans/growth & development , Caenorhabditis elegans Proteins/physiology , Cloning, Molecular , Collagen/physiology , Gene Dosage , Molecular Sequence Data , Neuropeptides/geneticsSubject(s)
Bacterial Outer Membrane Proteins/metabolism , GTPase-Activating Proteins/metabolism , Gastrointestinal Diseases/microbiology , Yersinia Infections/microbiology , Yersinia pseudotuberculosis/pathogenicity , Animals , Bacterial Outer Membrane Proteins/isolation & purification , Bacterial Toxins/metabolism , Bacteriological Techniques/methods , HeLa Cells , Humans , Mice , Recombinant Proteins/metabolism , Virulence , Yersinia pseudotuberculosis/growth & developmentABSTRACT
YopE of Yersinia pseudotuberculosis inactivates three members of the small RhoGTPase family (RhoA, Rac1 and Cdc42) in vitro and mutation of a critical arginine abolishes both in vitro GTPase-activating protein (GAP) activity and cytotoxicity towards HeLa cells, and renders the pathogen avirulent in a mouse model. To understand the functional role of YopE, in vivo studies of the GAP activity in infected eukaryotic cells were conducted. Wild-type YopE inactivated Rac1 as early as 5 min after infection whereas RhoA was down regulated about 30 min after infection. No effect of YopE was found on the activation state of Cdc42 in Yersinia-infected cells. Single-amino-acid substitution mutants of YopE revealed two different phenotypes: (i) mutants with significantly lowered in vivo GAP activity towards RhoA and Rac1 displaying full virulence in mice, and (ii) avirulent mutants with wild-type in vivo GAP activity towards RhoA and Rac1. Our results show that Cdc42 is not an in vivo target for YopE and that YopE interacts preferentially with Rac1, and to a lesser extent with RhoA, during in vivo conditions. Surprisingly, we present results suggesting that these interactions are not a prerequisite to establish infection in mice. Finally, we show that avirulent yopE mutants translocate YopE in about sixfold higher amount compared with wild type. This raises the question whether YopE's primary function is to sense the level of translocation rather than being directly involved in downregulation of the host defence.
Subject(s)
Bacterial Outer Membrane Proteins/analysis , Bacterial Outer Membrane Proteins/physiology , GTPase-Activating Proteins/analysis , GTPase-Activating Proteins/physiology , Yersinia pseudotuberculosis/chemistry , Yersinia pseudotuberculosis/physiology , Animals , Bacterial Outer Membrane Proteins/genetics , Bacterial Translocation/physiology , DNA, Bacterial/analysis , DNA, Bacterial/genetics , Down-Regulation/physiology , Female , Gene Expression Regulation, Bacterial , HeLa Cells , Humans , L-Lactate Dehydrogenase/metabolism , Mice , Mice, Inbred C57BL , Mutation , Substrate Specificity , Virulence , Yersinia pseudotuberculosis/pathogenicity , cdc42 GTP-Binding Protein/analysis , cdc42 GTP-Binding Protein/genetics , cdc42 GTP-Binding Protein/physiology , rac1 GTP-Binding Protein/analysis , rac1 GTP-Binding Protein/genetics , rac1 GTP-Binding Protein/physiology , rhoA GTP-Binding Protein/analysis , rhoA GTP-Binding Protein/genetics , rhoA GTP-Binding Protein/physiologyABSTRACT
The YopE cytotoxin of Yersinia is an essential virulence determinant that is translocated into the eukaryotic target cell via a plasmid-encoded type III secretion system. YopE possess a GTPase activating protein activity that in vitro has been shown to down regulate RhoA, Rac1, and Cdc42. Translocated YopE induces de-polymerisation of the actin microfilament structure in the eukaryotic cell which results in a rounding up of infected cells described as a cytotoxic effect. Here, we have investigated the importance of different regions of YopE for induction of cytotoxicity and in vitro GAP activity. Sequential removal of the N- and C-terminus of YopE identified the region between amino acids 90 and 215 to be necessary for induction of cytotoxicity. Internal deletions containing the essential arginine at position 144 resulted in a total loss of cytotoxic response. In-frame deletions flanking the arginine finger defined a region important for the cytotoxic effect to amino acids 166-183. Four triple-alanine substitution mutants in this region, YopE166-8A, 169-71A, 175-7A and 178-80A were still able to induce cytotoxicity on HeLa cells although they did not show any in vitro GAP activity towards RhoA, Rac1 or Cdc42. A substitution mutant in position 206-8A showed the same phenotype, ability to induce cytotoxic response but no in vitro GAP activity. We speculate that YopE may have additional unidentified targets within the eukaryotic cell.
Subject(s)
Bacterial Outer Membrane Proteins/toxicity , Bacterial Toxins/toxicity , GTPase-Activating Proteins/metabolism , rho GTP-Binding Proteins/metabolism , Amino Acid Sequence , Bacterial Outer Membrane Proteins/genetics , Cytotoxicity Tests, Immunologic , Cytotoxins/metabolism , Gene Deletion , HeLa Cells , Humans , Models, Molecular , Molecular Sequence Data , Mutation , Recombinant Proteins/analysis , Sequence Alignment , cdc42 GTP-Binding Protein/metabolism , rac1 GTP-Binding Protein/metabolism , rhoA GTP-Binding Protein/metabolismABSTRACT
14-3-3 proteins play an important role in a multitude of signalling pathways. The interactions between 14-3-3 and other signalling proteins, such as Raf and KSR (kinase suppressor of Ras), occur in a phospho-specific manner. Recently, a phosphorylation-independent interaction has been reported to occur between 14-3-3 and several proteins, for example 5-phosphatase, p75NTR-associated cell death executor (NADE) and the bacterial toxin Exoenzyme S (ExoS), an ADP-ribosyltransferase from Pseudomonas aeruginosa. In this study we have identified the amino acid residues on ExoS, which are responsible for its specific interaction with 14-3-3. Furthermore, we show that a peptide derived from ExoS, containing the 14-3-3 interaction site, effectively competes out the interaction between ExoS and 14-3-3. In addition, competition with this peptide blocks ExoS modification of Ras in our Ras modification assay. We show that the ExoS protein interacts with all isoforms of the 14-3-3 family tested. Moreover, in vivo an ExoS protein lacking the 14-3-3 binding site has a reduced capacity to ADP ribosylate cytoplasmic proteins, e.g. Ras, and shows a reduced capacity to change the morphology of infected cells.