ABSTRACT
Bio-guided fractionation of the 70% ethanol extract of Belamcanda chinensis (L.) DC. revealed four new compounds, including 6â³-O-acetylembinin (5), 3â³-O-acetylembinin (6), irigenin 3'-O-ß-glucopyranoside (8), and 2'-acetyl-1,3-O-diferuloylsucrose (9), along with five known compounds (1-4, 7). Their chemical structures were determined using extensive NMR data, mass spectroscopy, and comparison with published literature. Among the isolates, compounds 1 and 4-7 achieved good regulation of the growth and proliferation of vascular smooth muscle cells.
Subject(s)
Iridaceae/chemistry , Isoflavones/pharmacology , Muscle, Smooth, Vascular/cytology , Muscle, Smooth, Vascular/drug effects , Plant Components, Aerial/chemistry , Plant Extracts/pharmacology , Cell Proliferation/drug effects , Cells, Cultured , Dose-Response Relationship, Drug , Humans , Isoflavones/chemistry , Isoflavones/isolation & purification , Molecular Structure , Plant Extracts/chemistry , Plant Extracts/isolation & purification , Structure-Activity RelationshipABSTRACT
Four new triterpenoid saponins (gouaniasides VII-IX [1-3] and joazeiroside C [4]) and one known triterpenoid (5) were isolated from the aerial parts of Gouania leptostachya DC. (Rhamnaceae). Their structures were elucidated via one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy, high-resolution electrospray ionisation-mass spectrometry, and analyses of hydrolytic cleavage results. The anti-inflammatory potential of compounds 1-3 was evaluated according to their ability to inhibit the production of nitric oxide (NO) by RAW 264.7 macrophages. All compounds at noncytotoxic concentrations significantly inhibited NO production by macrophages in a concentration-dependent manner.
Subject(s)
Rhamnaceae , Saponins , Triterpenes , Nitric Oxide , Saponins/chemistry , Rhamnaceae/chemistry , Triterpenes/chemistry , Plant Components, Aerial/chemistry , Molecular StructureABSTRACT
Six undescribed sesquiterpene glucosides, fissispallins A-F, and one known sesquiterpene glucoside, fissispallin, were discovered in the leaves of Fissistigma pallens (Finet & Gagnep.) Merr. The structures were determined using spectroscopic methods, including 1D, 2D NMR, and MS. All compounds were evaluated for cytotoxic activity against three human cancer cell lines, HT-29, A-2058, and A-549. Fissispallin A showed potent activity with the IC50 values less than 1.5 µM against all tested human cancer cell lines. Fissispallin also showed potent activity with IC50 value of 0.4 ± 0.3 on the A-2058 cancer cell lines. Fissispallins B-D showed significant cytotoxic activity against all the tested cancer cell lines with IC50 values ranging from 3.8 to 7.2 µM.