ABSTRACT
According to in vitro assays, T cells are thought to kill rapidly and efficiently, but the efficacy and dynamics of cytotoxic T lymphocyte (CTL)-mediated killing of virus-infected cells in vivo remains elusive. We used two-photon microscopy to quantify CTL-mediated killing in mice infected with herpesviruses or poxviruses. On average, one CTL killed 2-16 virus-infected cells per day as determined by real-time imaging and by mathematical modeling. In contrast, upon virus-induced MHC class I downmodulation, CTLs failed to destroy their targets. During killing, CTLs remained migratory and formed motile kinapses rather than static synapses with targets. Viruses encoding the calcium sensor GCaMP6s revealed strong heterogeneity in individual CTL functional capacity. Furthermore, the probability of death of infected cells increased for those contacted by more than two CTLs, indicative of CTL cooperation. Thus, direct visualization of CTLs during killing of virus-infected cells reveals crucial parameters of CD8(+) T cell immunity.
Subject(s)
Herpesviridae Infections/immunology , Muromegalovirus/immunology , Perforin/metabolism , T-Lymphocyte Subsets/immunology , T-Lymphocytes, Cytotoxic/immunology , Vaccinia virus/immunology , Vaccinia/immunology , Animals , Calcium Signaling , Cell Communication , Cells, Cultured , Cytotoxicity, Immunologic , Humans , Immune Evasion , Mice , Mice, Inbred C57BL , Mice, Knockout , Microscopy, Fluorescence, Multiphoton , Perforin/genetics , T-Lymphocyte Subsets/virology , T-Lymphocytes, Cytotoxic/virologyABSTRACT
PURPOSE: KBG syndrome (KBGS) is a rare neurodevelopmental syndrome caused by haploinsufficiency of ANKRD11. The childhood phenotype is extensively reported but limited for adults. Thus, we aimed to delineate the clinical features of KBGS. METHODS: We collected physician-reported data of adults with molecularly confirmed KBGS through an international collaboration. Moreover, we undertook a systematic literature review to determine the scope of previously reported data. RESULTS: The international collaboration identified 36 adults from 31 unrelated families with KBGS. Symptoms included mild/borderline intellectual disability (n = 22); gross and/or fine motor difficulties (n = 15); psychiatric and behavioral comorbidities including aggression, anxiety, reduced attention span, and autistic features (n = 26); nonverbal (n = 3), seizures with various seizure types and treatment responses (n = 10); ophthalmological comorbidities (n = 20). Cognitive regression during adulthood was reported once. Infrequent features included dilatation of the ascending aorta (n = 2) and autoimmune conditions (n = 4). Education, work, and residence varied, and the diversity of professional and personal roles highlighted the range of abilities seen. The literature review identified 154 adults reported across the literature, and we have summarized the features across both data sets. CONCLUSION: Our study sheds light on the long-term neurodevelopmental outcomes, seizures, behavioral and psychiatric features, and education, work, and living arrangements for adults with KBGS.
Subject(s)
Intellectual Disability , Phenotype , Humans , Adult , Intellectual Disability/genetics , Intellectual Disability/epidemiology , Male , Female , Middle Aged , Young Adult , Haploinsufficiency/genetics , Seizures/genetics , Seizures/epidemiology , Physicians , Adolescent , Facies , Abnormalities, Multiple , Bone Diseases, Developmental , Tooth AbnormalitiesABSTRACT
Aminoacylation of transfer RNA (tRNA) is a key step in protein biosynthesis, carried out by highly specific aminoacyl-tRNA synthetases (ARSs). ARSs have been implicated in autosomal dominant and autosomal recessive human disorders. Autosomal dominant variants in tryptophanyl-tRNA synthetase 1 (WARS1) are known to cause distal hereditary motor neuropathy and Charcot-Marie-Tooth disease, but a recessively inherited phenotype is yet to be clearly defined. Seryl-tRNA synthetase 1 (SARS1) has rarely been implicated in an autosomal recessive developmental disorder. Here, we report five individuals with biallelic missense variants in WARS1 or SARS1, who presented with an overlapping phenotype of microcephaly, developmental delay, intellectual disability, and brain anomalies. Structural mapping showed that the SARS1 variant is located directly within the enzyme's active site, most likely diminishing activity, while the WARS1 variant is located in the N-terminal domain. We further characterize the identified WARS1 variant by showing that it negatively impacts protein abundance and is unable to rescue the phenotype of a CRISPR/Cas9 wars1 knockout zebrafish model. In summary, we describe two overlapping autosomal recessive syndromes caused by variants in WARS1 and SARS1, present functional insights into the pathogenesis of the WARS1-related syndrome and define an emerging disease spectrum: ARS-related developmental disorders with or without microcephaly.
Subject(s)
Amino Acyl-tRNA Synthetases , Charcot-Marie-Tooth Disease , Microcephaly , Tryptophan-tRNA Ligase , Animals , Humans , Amino Acyl-tRNA Synthetases/genetics , Charcot-Marie-Tooth Disease/genetics , Ligases , Microcephaly/genetics , Microcephaly/pathology , RNA, Transfer , Tryptophan-tRNA Ligase/genetics , Zebrafish/geneticsABSTRACT
The extensive clinical and genetic heterogeneity of congenital limb malformation calls for comprehensive genome-wide analysis of genetic variation. Genome sequencing (GS) has the potential to identify all genetic variants. Here we aim to determine the diagnostic potential of GS as a comprehensive one-test-for-all strategy in a cohort of undiagnosed patients with congenital limb malformations. We collected 69 cases (64 trios, 1 duo, 5 singletons) with congenital limb malformations with no molecular diagnosis after standard clinical genetic testing and performed genome sequencing. We also developed a framework to identify potential noncoding pathogenic variants. We identified likely pathogenic/disease-associated variants in 12 cases (17.4%) including four in known disease genes, and one repeat expansion in HOXD13. In three unrelated cases with ectrodactyly, we identified likely pathogenic variants in UBA2, establishing it as a novel disease gene. In addition, we found two complex structural variants (3%). We also identified likely causative variants in three novel high confidence candidate genes. We were not able to identify any noncoding variants. GS is a powerful strategy to identify all types of genomic variants associated with congenital limb malformation, including repeat expansions and complex structural variants missed by standard diagnostic approaches. In this cohort, no causative noncoding SNVs could be identified.
Subject(s)
Genetic Heterogeneity , Homeodomain Proteins/genetics , Limb Deformities, Congenital/genetics , Mutation , Transcription Factors/genetics , Ubiquitin-Activating Enzymes/genetics , Base Sequence , Cohort Studies , DNA Copy Number Variations , Gene Expression , Genetic Testing , Humans , Infant , Limb Deformities, Congenital/metabolism , Limb Deformities, Congenital/pathology , Male , Pedigree , Transcription Factors/deficiency , Ubiquitin-Activating Enzymes/deficiency , Whole Genome SequencingABSTRACT
Mutations affecting the transcriptional regulator Ankyrin Repeat Domain 11 (ANKRD11) are mainly associated with the multisystem developmental disorder known as KBG syndrome, but have also been identified in individuals with Cornelia de Lange syndrome (CdLS) and other developmental disorders caused by variants affecting different chromatin regulators. The extensive functional overlap of these proteins results in shared phenotypical features, which complicate the assessment of the clinical diagnosis. Additionally, re-evaluation of individuals at a later age occasionally reveals that the initial phenotype has evolved toward clinical features more reminiscent of a developmental disorder different from the one that was initially diagnosed. For this reason, variants in ANKRD11 can be ascribed to a broader class of disorders that fall within the category of the so-called chromatinopathies. In this work, we report on the clinical characterization of 23 individuals with variants in ANKRD11. The subjects present primarily with developmental delay, intellectual disability and dysmorphic features, and all but two received an initial clinical diagnosis of either KBG syndrome or CdLS. The number and the severity of the clinical signs are overlapping but variable and result in a broad spectrum of phenotypes, which could be partially accounted for by the presence of additional molecular diagnoses and distinct pathogenic mechanisms.
Subject(s)
Abnormalities, Multiple/etiology , Bone Diseases, Developmental/etiology , Intellectual Disability/etiology , Repressor Proteins/genetics , Tooth Abnormalities/etiology , Abnormalities, Multiple/genetics , Adolescent , Bone Diseases, Developmental/genetics , Child , Child, Preschool , Face/abnormalities , Facies , Female , Humans , Intellectual Disability/genetics , Male , Mutation , Pedigree , Tooth Abnormalities/genetics , Young AdultABSTRACT
Patients with Van der Woude syndrome typically present with cleft lip, cleft lip and palate, or with cleft palate only. In contrast to non-syndromic cleft lip and/or palate, Van der Woude syndrome typically is characterized by bilateral, paramedian lower-lip pits. Popliteal pterygium syndrome shares features with Van der Woude syndrome, but, in addition, is characterized by a popliteal pterygium, genital anomalies, cutaneous syndactyly of the fingers and the toes, and a characteristic pyramidal fold of skin overlying the nail of the hallux. In some patients oral synechiae or eyelid synechiae are present. Van der Woude Syndrome and Popliteal pterygium syndrome are autosomal dominantly inherited disorders caused by heterozygous mutations in IRF6. We present a three generation family with tremendous intrafamilial phenotypic variability. The newborn index patient had a diagnosis of Popliteal pterygium syndrome. The mother presented with a classic Van der Woude Syndrome, while the maternal grandfather had Van der Woude Syndrome as well as minor signs of Popliteal pterygium syndrome. In all three affecteds the known pathogenic mutation c.265A>G, p.Lys89Glu in IRF6 was identified. While inter- as well as intra-familial variability has been described in IRF6-related disorders, the occurrence of a typical Van der Woude Syndrome without any other anomalies as well as a diagnosis of Popliteal pterygium syndrome in the same family is rare. © 2016 Wiley Periodicals, Inc.
Subject(s)
Abnormalities, Multiple/diagnosis , Abnormalities, Multiple/genetics , Cleft Lip/diagnosis , Cleft Lip/genetics , Cleft Palate/diagnosis , Cleft Palate/genetics , Cysts/diagnosis , Cysts/genetics , Eye Abnormalities/diagnosis , Eye Abnormalities/genetics , Fingers/abnormalities , Interferon Regulatory Factors/genetics , Knee Joint/abnormalities , Lip/abnormalities , Lower Extremity Deformities, Congenital/diagnosis , Lower Extremity Deformities, Congenital/genetics , Mutation , Phenotype , Syndactyly/diagnosis , Syndactyly/genetics , Urogenital Abnormalities/diagnosis , Urogenital Abnormalities/genetics , Adult , Alleles , Exons , Female , Genetic Association Studies , Heterozygote , Humans , Infant, Newborn , Male , PedigreeABSTRACT
Due to a unique pattern of CD8 T-cell response induced by cytomegaloviruses (CMVs), live attenuated CMVs are attractive candidates for vaccine vectors for a number of clinically relevant infections and tumors. NKG2D is one of the most important activating NK cell receptors that plays a role in costimulation of CD8 T cells. Here we demonstrate that the expression of CD8 T-cell epitope of Listeria monocytogenes by a recombinant mouse CMV (MCMV) expressing the NKG2D ligand retinoic acid early-inducible protein 1-gamma (RAE-1γ) dramatically enhanced the effectiveness and longevity of epitope-specific CD8 T-cell response and conferred protection against a subsequent challenge infection with Listeria monocytogenes. Unexpectedly, the attenuated growth in vivo of the CMV vector expressing RAE-1γ and its capacity to enhance specific CD8 T-cell response were preserved even in mice lacking NKG2D, implying additional immune function for RAE-1γ beyond engagement of NKG2D. Thus, vectors expressing RAE-1γ represent a promising approach in the development of CD8 T-cell-based vaccines.
Subject(s)
CD8-Positive T-Lymphocytes/immunology , Cytomegalovirus/immunology , Genetic Vectors/immunology , Immune Evasion/immunology , Membrane Proteins/metabolism , Vaccines, Synthetic/immunology , Animals , Cytomegalovirus/genetics , Flow Cytometry , Genetic Vectors/genetics , Listeria monocytogenes/immunology , Membrane Proteins/genetics , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Knockout , NK Cell Lectin-Like Receptor Subfamily K/genetics , Statistics, NonparametricABSTRACT
Variants in cullin 4B (CUL4B) are a known cause of syndromic X-linked intellectual disability. Here, we describe an additional 25 patients from 11 families with variants in CUL4B. We identified nine different novel variants in these families and confirmed the pathogenicity of all nontruncating variants. Neuroimaging data, available for 15 patients, showed the presence of cerebral malformations in ten patients. The cerebral anomalies comprised malformations of cortical development (MCD), ventriculomegaly, and diminished white matter volume. The phenotypic heterogeneity of the cerebral malformations might result from the involvement of CUL-4B in various cellular pathways essential for normal brain development. Accordingly, we show that CUL-4B interacts with WDR62, a protein in which variants were previously identified in patients with microcephaly and a wide range of MCD. This interaction might contribute to the development of cerebral malformations in patients with variants in CUL4B.
Subject(s)
Brain/pathology , Cullin Proteins/genetics , Cullin Proteins/metabolism , Malformations of Cortical Development/genetics , Mental Retardation, X-Linked/genetics , Nerve Tissue Proteins/metabolism , Adolescent , Adult , Cell Cycle Proteins , Cells, Cultured , Child , Child, Preschool , Genetic Association Studies , HEK293 Cells , Humans , Infant , Male , Malformations of Cortical Development/metabolism , Malformations of Cortical Development/pathology , Mental Retardation, X-Linked/metabolism , Mental Retardation, X-Linked/pathology , Middle Aged , Pedigree , Sequence Analysis, DNA , Young AdultABSTRACT
Neonates, including mice and humans, are highly susceptible to cytomegalovirus (CMV) infection. However, many aspects of neonatal CMV infections such as viral cell tropism, spatio-temporal distribution of the pathogen as well as genesis of antiviral immunity are unknown. With the use of reporter mutants of the murine cytomegalovirus (MCMV) we identified the lung as a primary target of mucosal infection in neonatal mice. Comparative analysis of neonatal and adult mice revealed a delayed control of virus replication in the neonatal lung mucosa explaining the pronounced systemic infection and disease in neonates. This phenomenon was supplemented by a delayed expansion of CD8(+) T cell clones recognizing the viral protein M45 in neonates. We detected viral infection at the single-cell level and observed myeloid cells forming "nodular inflammatory foci" (NIF) in the neonatal lung. Co-localization of infected cells within NIFs was associated with their disruption and clearance of the infection. By 2-photon microscopy, we characterized how neonatal antigen-presenting cells (APC) interacted with T cells and induced mature adaptive immune responses within such NIFs. We thus define NIFs of the neonatal lung as niches for prolonged MCMV replication and T cell priming but also as sites of infection control.
Subject(s)
Cytomegalovirus Infections/immunology , Lung/immunology , Muromegalovirus/immunology , Pneumonia/immunology , Pneumonia/virology , T-Lymphocytes/immunology , Animals , Animals, Newborn , Antigen Presentation , Cells, Cultured , Cytomegalovirus Infections/complications , Cytomegalovirus Infections/pathology , Cytomegalovirus Infections/virology , Intestines/immunology , Intestines/pathology , Intestines/virology , Lung/growth & development , Lung/pathology , Lung/virology , Lymphocyte Activation , Mice , Mice, Inbred C57BL , Mice, Transgenic , Muromegalovirus/growth & development , Pneumonia/pathologyABSTRACT
CMV can infect dendritic cells (DCs), and direct Ag presentation could, therefore, lead to the priming of CMV-specific CD8(+) T cells. However, CMV-encoded immune evasins severely impair Ag presentation in the MHC class I pathway; thus, it is widely assumed that cross-presentation drives the priming of antiviral T cells. We assessed the contribution of direct versus cross priming in mouse CMV (MCMV) infection using recombinant viruses. DCs infected with an MCMV strain encoding the gB498 epitope from HSV-1 were unable to stimulate in vitro naive gB498-specific CD8(+) T cells from TCR transgenic mice. Infection of C57BL/6 mice with this recombinant virus led, however, to the generation of abundant numbers of gB498-specific T cells in vivo. Of the DC subsets isolated from infected mice, only CD8α(+) DCs were able to stimulate naive T cells, suggesting that this DC subset cross-presents MCMV-encoded Ag in vivo. Upon infection of mice with MCMV mutants encoding Ag that can either be well or hardly cross-presented, mainly CD8(+) T cells specific for cross-presented epitopes were generated. Moreover, even in the absence of immune evasion genes interfering with MHC class I-mediated Ag presentation, priming of T cells to Ag that can only be presented directly was not observed. We conclude that the host uses mainly DCs capable of cross-presentation to induce the CMV-specific CD8(+) T cell response during primary, acute infection and discuss the implications for the development of a CMV vaccine.
Subject(s)
Antigens, Viral/immunology , CD8-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/virology , Cross-Priming/immunology , Herpesviridae Infections/immunology , Lymphocyte Activation/immunology , Muromegalovirus/immunology , Animals , Antigen-Presenting Cells/immunology , Antigen-Presenting Cells/virology , CD8-Positive T-Lymphocytes/pathology , Clone Cells , Epitopes, T-Lymphocyte/immunology , Female , Herpesviridae Infections/pathology , Herpesviridae Infections/virology , Mice , Mice, Inbred C57BL , Mice, Transgenic , Muromegalovirus/geneticsABSTRACT
Little is known about the role of viral genes in modulating host cytokine responses. Here we report a new functional role of the viral encoded IE1 protein of the murine cytomegalovirus in sculpting the inflammatory response in an acute infection. In time course experiments of infected primary macrophages (MΦs) measuring cytokine production levels, genetic ablation of the immediate-early 1 (ie1) gene results in a significant increase in TNFα production. Intracellular staining for cytokine production and viral early gene expression shows that TNFα production is highly associated with the productively infected MΦ population of cells. The ie1- dependent phenotype of enhanced MΦ TNFα production occurs at both protein and RNA levels. Noticeably, we show in a series of in vivo infection experiments that in multiple organs the presence of ie1 potently inhibits the pro-inflammatory cytokine response. From these experiments, levels of TNFα, and to a lesser extent IFNß, but not the anti-inflammatory cytokine IL10, are moderated in the presence of ie1. The ie1- mediated inhibition of TNFα production has a similar quantitative phenotype profile in infection of susceptible (BALB/c) and resistant (C57BL/6) mouse strains as well as in a severe immuno-ablative model of infection. In vitro experiments with infected macrophages reveal that deletion of ie1 results in increased sensitivity of viral replication to TNFα inhibition. However, in vivo infection studies show that genetic ablation of TNFα or TNFRp55 receptor is not sufficient to rescue the restricted replication phenotype of the ie1 mutant virus. These results provide, for the first time, evidence for a role of IE1 as a regulator of the pro-inflammatory response and demonstrate a specific pathogen gene capable of moderating the host production of TNFα in vivo.
Subject(s)
Gene Expression Regulation, Viral/genetics , Herpesviridae Infections/immunology , Immediate-Early Proteins/genetics , Muromegalovirus/genetics , Tumor Necrosis Factor-alpha/metabolism , Animals , Cell Line , Cytokines/metabolism , DNA Replication , DNA, Viral/genetics , Female , Herpesviridae Infections/virology , Immediate-Early Proteins/metabolism , Liver/metabolism , Macrophages/metabolism , Male , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Muromegalovirus/growth & development , Muromegalovirus/physiology , Phenotype , Signal Transduction , Tumor Necrosis Factor-alpha/analysis , Tumor Necrosis Factor-alpha/genetics , Viral Proteins/genetics , Viral Proteins/metabolism , Virus ReplicationABSTRACT
OFD1, now recognized as a ciliopathy, is characterized by malformations of the face, oral cavity and digits, and is transmitted as an X-linked condition with lethality in males. Mutations in OFD1 also cause X-linked Joubert syndrome (JBTS10) and Simpson-Golabi-Behmel syndrome type 2 (SGBS2). We have studied 55 sporadic and six familial cases of suspected OFD1. Comprehensive mutation analysis in OFD1 revealed mutations in 37 female patients from 30 families; 22 mutations have not been previously described including two heterozygous deletions spanning OFD1 and neighbouring genes. Analysis of clinical findings in patients with mutations revealed that oral features are the most reliable diagnostic criteria. A first, detailed evaluation of brain MRIs from seven patients with cognitive defects illustrated extensive variability with the complete brain phenotype consisting of complete agenesis of the corpus callosum, large single or multiple interhemispheric cysts, striking cortical infolding of gyri, ventriculomegaly, mild molar tooth malformation and moderate to severe cerebellar vermis hypoplasia. Although the OFD1 gene apparently escapes X-inactivation, skewed inactivation was observed in seven of 14 patients. The direction of skewing did not correlate with disease severity, reinforcing the hypothesis that additional factors contribute to the extensive intrafamilial variability.
Subject(s)
Gene Deletion , Mutation , Orofaciodigital Syndromes/genetics , Proteins/genetics , Adolescent , Alternative Splicing/genetics , Base Sequence , Brain/metabolism , Brain/pathology , Child , DNA Mutational Analysis , Exons/genetics , Family Health , Female , Genetic Association Studies/methods , Humans , Infant , Introns/genetics , Magnetic Resonance Imaging , Male , Orofaciodigital Syndromes/pathology , Pedigree , X Chromosome InactivationABSTRACT
Mouse CMV (MCMV) infection rapidly induces the proliferation of NK cells, which correlates with immunological protection. Whether NK cells primed during acute response against MCMV are maintained for the long term is not known. In this study, we used TcrdH2BeGFP mice in which maturing NK cells are genetically labeled with a pulse of very stable histone-2B-eGFP. In this system, we found that the reporter protein was diluted out upon NK cell division during acute MCMV infection. At the same time, mature NK cells in uninfected mice showed only very limited turnover in vivo. Three months after primary infection when MCMV latency was established, the majority of peripheral NK cells still displayed a higher record of proliferation than NK cells in mock-infected controls. This observation included both Ly49H(+) and Ly49H(-) NK cells. Conversely, naive NK cells did not show more proliferation after transfer into latently MCMV-infected mice than that after transfer into mock-infected control mice. This indicated that the observed alterations of the NK cell compartment in MCMV latency were "legacy" (i.e., resulting from prior events during the initial immune response). Together, these results suggest that antiviral immune responses induce sustained alterations of innate lymphocyte populations that extend far beyond the first days of acute infection.
Subject(s)
Cytomegalovirus Infections/immunology , Cytomegalovirus Infections/virology , Immunity, Innate/genetics , Killer Cells, Natural/immunology , Killer Cells, Natural/virology , Virus Latency/immunology , Acute Disease , Animals , Cytomegalovirus Infections/pathology , Green Fluorescent Proteins/genetics , Histones/genetics , Killer Cells, Natural/metabolism , Mice , Mice, Inbred C57BL , Mice, Transgenic , Muromegalovirus/genetics , Muromegalovirus/immunology , NK Cell Lectin-Like Receptor Subfamily A/biosynthesis , NK Cell Lectin-Like Receptor Subfamily A/deficiency , NK Cell Lectin-Like Receptor Subfamily A/physiology , Virus Latency/geneticsABSTRACT
Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.
Subject(s)
Genes, Essential , Genes, Viral , Herpesviridae/genetics , Viral Proteins/genetics , Animals , Cytomegalovirus/genetics , Fibroblasts/virology , Genetic Complementation Test , Genome, Viral , Herpesviridae/drug effects , Herpesviridae/metabolism , Humans , Ligands , Mice , Morpholines/pharmacology , Mutation , Protein Stability , Recombinant Fusion Proteins/genetics , Virus Replication/drug effects , Virus Replication/geneticsABSTRACT
Cytomegaloviruses (CMV) utilize a variety of immunomodulatory strategies to facilitate the establishment of lifelong persistence in their infected hosts. We show that the mouse CMV (MCMV) m155 open reading frame (ORF) is required for the posttranscriptional inhibition of CD40 expression in infected antigen-presenting cells. Consistent with the known importance of CD40-mediated costimulation of T cells, a m155-deficient virus induces enhanced MCMV epitope-specific CD4 T cell responses.
Subject(s)
CD4-Positive T-Lymphocytes/immunology , CD40 Antigens/antagonists & inhibitors , Glycoproteins/metabolism , Muromegalovirus/immunology , Muromegalovirus/pathogenicity , Viral Proteins/metabolism , Animals , Glycoproteins/immunology , Mice , Viral Proteins/immunologyABSTRACT
Activated macrophages play a central role in controlling inflammatory responses to infection and are tightly regulated to rapidly mount responses to infectious challenge. Type I interferon (alpha/beta interferon [IFN-α/ß]) and type II interferon (IFN-γ) play a crucial role in activating macrophages and subsequently restricting viral infections. Both types of IFNs signal through related but distinct signaling pathways, inducing a vast number of interferon-stimulated genes that are overlapping but distinguishable. The exact mechanism by which IFNs, particularly IFN-γ, inhibit DNA viruses such as cytomegalovirus (CMV) is still not fully understood. Here, we investigate the antiviral state developed in macrophages upon reversible inhibition of murine CMV by IFN-γ. On the basis of molecular profiling of the reversible inhibition, we identify a significant contribution of a restricted type I IFN subnetwork linked with IFN-γ activation. Genetic knockout of the type I-signaling pathway, in the context of IFN-γ stimulation, revealed an essential requirement for a primed type I-signaling process in developing a full refractory state in macrophages. A minimal transient induction of IFN-ß upon macrophage activation with IFN-γ is also detectable. In dose and kinetic viral replication inhibition experiments with IFN-γ, the establishment of an antiviral effect is demonstrated to occur within the first hours of infection. We show that the inhibitory mechanisms at these very early times involve a blockade of the viral major immediate-early promoter activity. Altogether our results show that a primed type I IFN subnetwork contributes to an immediate-early antiviral state induced by type II IFN activation of macrophages, with a potential further amplification loop contributed by transient induction of IFN-ß.
Subject(s)
Interferon Type I/immunology , Interferon-gamma/immunology , Macrophages/immunology , Macrophages/virology , Muromegalovirus/growth & development , Muromegalovirus/immunology , Animals , Macrophage Activation , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Signal Transduction , Time FactorsABSTRACT
The molecular mechanisms leading to reactivation of latent cytomegalovirus are not well understood. To study reactivation, the few cells in an organ tissue that give rise to reactivated virus need to be identified, ideally at the earliest possible time point in the process. To this end, mouse cytomegalovirus (MCMV) reporter mutants were designed to simultaneously express the red fluorescent protein mCherry and the secreted Gaussia luciferase (Gluc). Whereas Gluc can serve to assess infection at the level of individual mice by measuring luminescence in blood samples or by in vivo imaging, mCherry fluorescence offers the advatage of detection of infection at the single cell level. To visualize cells in which MCMV was being reactivated, precision-cut lung slices (PCLS) that preserve tissue microanatomy were prepared from the lungs of latently infected mice. By day 3 of cultivation of the PCLS, reactivation was revealed by Gluc expression, preceding the detection of infectious virus by approximately 4 days. Reactivation events in PCLS could be identified when they were still confined to single cells. Notably, using fractalkine receptor-GFP reporter mice, we never observed reactivation originating from CX3CR1(+) monocytes or pulmonary dendritic cells derived therefrom. Furthermore, latent viral genome in the lungs was not enriched in sorted bone-marrow-derived cells expressing CD11b. Taken together, these complementary approaches suggest that CD11b(+) and CX3CR1(+) subsets of the myeloid differentiation lineage are not the main reservoirs and cellular sites of MCMV latency and reactivation in the lungs.
Subject(s)
Cytomegalovirus Infections/virology , Muromegalovirus/physiology , Single-Cell Analysis/methods , Virus Activation , Virus Latency , Animals , Cytomegalovirus/genetics , Cytomegalovirus/physiology , Disease Models, Animal , Female , Gene Expression , Genes, Reporter , Herpesviridae Infections/virology , Host-Pathogen Interactions , Humans , Luciferases/genetics , Luciferases/metabolism , Luminescent Proteins/genetics , Luminescent Proteins/metabolism , Lung/cytology , Lung/virology , Male , Mice , Mice, Inbred BALB C , Muromegalovirus/genetics , Muromegalovirus/isolation & purification , Red Fluorescent ProteinABSTRACT
The immediate-early protein IE1 of human and mouse cytomegalovirus (MCMV) is one of the first proteins expressed during the productive infection cycle and upon reactivation from latency. The CMV IE1 proteins have been found to inhibit histone deacetylases, suggesting a role in the epigenetic regulation of viral gene expression. Consequently, the IE1 protein is considered to have a profound effect on reactivation, because small amounts of IE1 may be decisive for the switch to lytic replication. Here we asked if an MCMV Deltaie1 mutant is able both to establish latency and to reactivate from the lungs of latently infected mice. Since the Deltaie1 mutant was known to be attenuated during acute infection, we first defined conditions that led to comparable levels of viral genomes during latent infection with mutant and wild-type (wt) MCMV. Viral genome copy numbers dropped considerably at the onset of the latent infection but then remained steady for both viruses even after several months. Reactivation of the Deltaie1 mutant and of wt MCMV from latency occurred with similar incidences in lung explant cultures at 4, 7, and 12 months postinfection. The increase in the frequency of a subset of MCMV-specific memory T cells, a possible indicator of frequent transcriptional reactivation events during latency, was in a comparable range for both viruses. Recurrence of the Deltaie1 virus infection in vivo could also be induced by hematoablative treatment of latently infected mice. We conclude that the ie1 gene is not essential for the establishment of latency or for the reactivation of MCMV.
Subject(s)
Herpesviridae Infections/metabolism , Herpesviridae Infections/virology , Immediate-Early Proteins/metabolism , Lung/virology , Muromegalovirus/physiology , Virus Activation/physiology , Virus Latency/physiology , Animals , Cells, Cultured , Female , Gene Deletion , Genome, Viral/genetics , Immediate-Early Proteins/genetics , Mice , Mice, Inbred BALB C , Muromegalovirus/genetics , Mutation/genetics , Time FactorsABSTRACT
The human cytomegalovirus (HCMV) major immediate-early enhancer has been postulated to play a pivotal role in the control of latency and reactivation. However, the absence of an animal model has obstructed a direct test of this hypothesis. Here we report on the establishment of an in vivo, experimentally tractable system for quantitatively investigating physiological functions of the HCMV enhancer. Using a neonate BALB/c mouse model, we show that a chimeric murine CMV under the control of the HCMV enhancer is competent in vivo, replicating in key organs of mice with acute CMV infection and exhibiting latency/reactivation features comparable for the most part to those of the parental and revertant viruses.
Subject(s)
Cytomegalovirus Infections/genetics , Cytomegalovirus/genetics , Enhancer Elements, Genetic , Immediate-Early Proteins/genetics , Muromegalovirus , Virus Activation/physiology , Virus Latency/physiology , Animals , Animals, Newborn , Cell Line , Cytomegalovirus/metabolism , Cytomegalovirus Infections/metabolism , Cytomegalovirus Infections/pathology , Disease Models, Animal , Female , Humans , Immediate-Early Proteins/metabolism , Mice , Mice, Inbred BALB C , Muromegalovirus/genetics , Muromegalovirus/metabolism , Muromegalovirus/pathogenicity , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Virus ReplicationABSTRACT
INTRODUCTION: Multiple sulfatase deficiency is biochemically characterized by the accumulation of sulfated lipids and acid mucopolysaccharides. CASE REPORT: We report clinical, biochemical, and molecular findings in a female newborn affected with a severe form of multiple sulfatase deficiency (Mendelian Inheritance in Man (MIM) # 272200). She presented with primary microcephaly, facial anomalies including depressed nasal bridge, nasal hypoplasia, anteverted nostrils, smooth philtrum, limited mobility of hip and knee joints, mild ichthyosis, as well as muscular hypotonia. The diagnosis is based on detection of excessive mucopolysacchariduria and enzymatic assays performed in leucocytes which showed complete deficiency of all of the measured sulfatases. Sequencing of the coding region of the underlying gene, SUMF1, could not identify any mutation. However, failure to detect the corresponding mRNA by reverse transcription polymerase chain reaction proves defective SUMF1 expression. CONCLUSION: The diagnosis of neonatal MSD should be considered when dealing with the association of distinct facial anomalies, limited joint mobility, ichthyosis, and muscular hypotonia.