Stop! Graphic Health Warnings Enhance Inhibitory Control in Adolescents: An Event-Related Potential Study.

INTRODUCTION: The majority of smokers begin consumption in adolescence and the earlier initiation of cigarette smoking is associated with a greater likelihood of cigarette dependence. Graphic health warnings (GHW) are one of the most used strategies to communicate the consequences of cigarette smoking, but little is known about their ability to increase inhibitory control and thus prevent consumption. The objective of the present study was to evaluate the effects of different sizes of GHWs on inhibitory control in adolescents. We hypothesized that GHWs promote inhibitory control, and increasing GHW size, enhance inhibitory control. METHODS: Fifty-nine participants completed a Go/No-Go task during electroencephalographic recording. The No-Go stimuli were pictures of cigarette packs without GHWs, and cigarette packs with GHWs that covered 30% or 60% of the front (main side) of the pack. The event-related potential N200 component and behavioral measures in the Go/No-Go task were analyzed. RESULTS: Separate mixed-model analysis of variance (ANOVAs) were used for N200 component (amplitude and latency) and behavioral data. The GHWs increased the amplitude of the N200 potential, especially GHWs that covered 60% of the front of the pack. The behavioral data showed that GHWs that covered 60% of the front of the pack generated higher a percentage of accuracy in No-Go trials (ie, fewer commission errors). CONCLUSIONS: These results suggest that GHWs increase inhibitory control in adolescents, especially when the GHWs cover 60% of the front of the cigarette pack. IMPLICATIONS: GHWs with an increased size (60% of the front of the cigarette pack vs. 30%, the minimum size, proposed by the World Health Organization) recruit additional cognitive resources and thus can effectively increase inhibitory control both in adolescent smokers and nonsmokers. Accordingly, the use of larger GHW has the potential of becoming an effective public policy strategy to inhibit smoking in adolescents.

The effect of information seeking behaviors in fear control.

BACKGROUND AND OBJECTIVES: Individuals experiencing conditioned fear reactions often resort to avoidance and escape behaviors as attempts to decrease fear. Nevertheless, these strategies are not always available. In such cases, people seek information to mitigate aversive events. This study aimed at evaluating the effect of information seeking behaviors on self-reported fear, predictability, and physiological responses. METHODS: Participants were randomly assigned to two groups. In group one, individuals were given the choice to perform an instrumental behavior which provided information about the occurrence of either an aversive or a neutral event (100% contingency). In group two, individuals were also allowed to perform an instrumental behavior. However, such behavior provided partial information (50% contingency). RESULTS: Individuals in group one presented lower levels of fear compared to individuals assigned to group two. LIMITATIONS: The generalizability of the results may be restricted to undergraduate students. CONCLUSIONS: The results suggests that when avoidance and escape are not available, individuals seek information that provides control over fear. Clinical implications are discussed.

Differentiation-state plasticity is a targetable resistance mechanism in basal-like breast cancer.

Intratumoral heterogeneity in cancers arises from genomic instability and epigenomic plasticity and is associated with resistance to cytotoxic and targeted therapies. We show here that cell-state heterogeneity, defined by differentiation-state marker expression, is high in triple-negative and basal-like breast cancer subtypes, and that drug tolerant persister (DTP) cell populations with altered marker expression emerge during treatment with a wide range of pathway-targeted therapeutic compounds. We show that MEK and PI3K/mTOR inhibitor-driven DTP states arise through distinct cell-state transitions rather than by Darwinian selection of preexisting subpopulations, and that these transitions involve dynamic remodeling of open chromatin architecture. Increased activity of many chromatin modifier enzymes, including BRD4, is observed in DTP cells. Co-treatment with the PI3K/mTOR inhibitor BEZ235 and the BET inhibitor JQ1 prevents changes to the open chromatin architecture, inhibits the acquisition of a DTP state, and results in robust cell death in vitro and xenograft regression in vivo.

Exome Sequencing of Cell-Free DNA from Metastatic Cancer Patients Identifies Clinically Actionable Mutations Distinct from Primary Disease.

The identification of the molecular drivers of cancer by sequencing is the backbone of precision medicine and the basis of personalized therapy; however, biopsies of primary tumors provide only a snapshot of the evolution of the disease and may miss potential therapeutic targets, especially in the metastatic setting. A liquid biopsy, in the form of cell-free DNA (cfDNA) sequencing, has the potential to capture the inter- and intra-tumoral heterogeneity present in metastatic disease, and, through serial blood draws, track the evolution of the tumor genome. In order to determine the clinical utility of cfDNA sequencing we performed whole-exome sequencing on cfDNA and tumor DNA from two patients with metastatic disease; only minor modifications to our sequencing and analysis pipelines were required for sequencing and mutation calling of cfDNA. The first patient had metastatic sarcoma and 47 of 48 mutations present in the primary tumor were also found in the cell-free DNA. The second patient had metastatic breast cancer and sequencing identified an ESR1 mutation in the cfDNA and metastatic site, but not in the primary tumor. This likely explains tumor progression on Anastrozole. Significant heterogeneity between the primary and metastatic tumors, with cfDNA reflecting the metastases, suggested separation from the primary lesion early in tumor evolution. This is best illustrated by an activating PIK3CA mutation (H1047R) which was clonal in the primary tumor, but completely absent from either the metastasis or cfDNA. Here we show that cfDNA sequencing supplies clinically actionable information with minimal risks compared to metastatic biopsies. This study demonstrates the utility of whole-exome sequencing of cell-free DNA from patients with metastatic disease. cfDNA sequencing identified an ESR1 mutation, potentially explaining a patient's resistance to aromatase inhibition, and gave insight into how metastatic lesions differ from the primary tumor.

Caracteristicas de la respuesta emocional generada por las palabras: un estudio experimental desde la emoción y la motivación / Characteristics of emotional response generated by words: an experimental study from emotion and motivation

Resumen Los estudios experimentales de la emoción y la motivación se han desarrollado de forma más frecuente con imágenes, sonidos y videos, sin embargo, son pocos los trabajos que han estudiado la respuesta emocional y motivacional ante las palabras, las cuales son estímulos que guían significativamente nuestra interacción social. Por lo anterior, el objetivo de la presente investigación fue identificar la capacidad que tienen las palabras para generar estados emocionales y las posibles diferencias entre hombres y mujeres. Para esto se llevó a cabo un estudio con 232 personas y se utilizaron 15 palabras con contenido afectivo, las cuales fueron evaluadas en las dimensiones de valencia, arousal y dominancia. Los resultados muestran que las palabras agradables, en especial aquellas con contenido sexual generan una valencia apetitiva, alto arousal y alta dominancia, y las palabras desagradables generan una valencia aversiva, bajo arousal y baja dominancia. Solo se encontraron diferencias significativas entre hombres y mujeres en la valencia de las palabras desagradables y el arousal de las palabras agradables.

Abstract The experimental studies of emotion and motivation have developed more often with pictures, sounds and videos; however, few studies have addressed the emotional and motivational response to the words, which are stimuli that guide significantly our social interaction. Therefore, the objective of this research was to identify the words capacity to generate emotional states and differences between men and women. For this we conducted a study with 232 persons and used 15 words with affective content which were evaluated in the dimensions of valence, arousal and dominance. The results show that pleasant words, especially those with sexual content generate an appetitive valence, high arousal and high dominance, and unpleasant words generate an aversive valence, low arousal and low dominance. Significant differences between men and women only found in valence of unpleasant words and in arousal of the pleasant words.