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1.
J Infect Dis ; 230(3): 598-605, 2024 Sep 23.
Article in English | MEDLINE | ID: mdl-38373258

ABSTRACT

A statewide genomic surveillance system for invasive Group A Streptococcus was implemented in Arizona in June 2019, resulting in 1046 isolates being submitted for genomic analysis to characterize emm types and identify transmission clusters. Eleven of the 32 identified distinct emm types comprised >80% of samples, with 29.7% of all isolates being typed as emm49 (and its genetic derivative emm151). Phylogenetic analysis initially identified an emm49 genomic cluster of 4 isolates that rapidly expanded over subsequent months (June 2019 to February 2020). Public health investigations identified epidemiologic links with 3 different long-term care facilities, resulting in specific interventions. Unbiased genomic surveillance allowed for identification and response to clusters that would have otherwise remained undetected.


Subject(s)
Phylogeny , Streptococcal Infections , Streptococcus pyogenes , Arizona/epidemiology , Humans , Streptococcus pyogenes/genetics , Streptococcus pyogenes/classification , Streptococcus pyogenes/isolation & purification , Streptococcal Infections/epidemiology , Streptococcal Infections/microbiology , Female , Adult , Male , Child, Preschool , Child , Middle Aged , Aged , Young Adult , Adolescent , Health Facilities , Infant , Aged, 80 and over , Bacterial Outer Membrane Proteins/genetics , Genomics , Epidemiological Monitoring , Infant, Newborn , Genome, Bacterial , Antigens, Bacterial/genetics
2.
Emerg Infect Dis ; 28(1): 51-61, 2022 01.
Article in English | MEDLINE | ID: mdl-34932447

ABSTRACT

Carbapenem-resistant Pseudomonas aeruginosa (CRPA) producing the Verona integron‒encoded metallo-ß-lactamase (VIM) are highly antimicrobial drug-resistant pathogens that are uncommon in the United States. We investigated the source of VIM-CRPA among US medical tourists who underwent bariatric surgery in Tijuana, Mexico. Cases were defined as isolation of VIM-CRPA or CRPA from a patient who had an elective invasive medical procedure in Mexico during January 2018‒December 2019 and within 45 days before specimen collection. Whole-genome sequencing of isolates was performed. Thirty-eight case-patients were identified in 18 states; 31 were operated on by surgeon 1, most frequently at facility A (27/31 patients). Whole-genome sequencing identified isolates linked to surgeon 1 were closely related and distinct from isolates linked to other surgeons in Tijuana. Facility A closed in March 2019. US patients and providers should acknowledge the risk for colonization or infection after medical tourism with highly drug-resistant pathogens uncommon in the United States.


Subject(s)
Drug Resistance, Multiple, Bacterial , Medical Tourism , Pseudomonas Infections , Anti-Bacterial Agents/therapeutic use , Bacterial Proteins , Carbapenems , Humans , Mexico/epidemiology , Microbial Sensitivity Tests , Pseudomonas Infections/epidemiology , Pseudomonas Infections/microbiology , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/genetics , United States/epidemiology , beta-Lactamases/genetics
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