Evaluating antimicrobial, cytotoxic and immunomodulatory effects of glass ionomer cement modified by chitosan and hydroxyapatite.

OBJECTIVES: This study aimed to assess antimicrobial efficacy, cytotoxicity, and cytokine release (IL-1b, IL-6, IL-10, TNF-α) from human dental pulp stem cells (hDPSCs) of chitosan (CH) and hydroxyapatite (HAp)-modified glass ionomer cements (GIC). METHODS: GICs with varied CH and HAp concentrations (0 %, 0.16 %, 2 %, 5 %, 10 %) were tested against S. mutans for 24 h or 7 days. Antimicrobial activity was measured using an MTT test. Cytotoxicity evaluation followed for optimal concentrations, analyzing mitochondrial activity and apoptosis in hDPSCs. Cytokine release was assessed with MAGPIX. Antimicrobial analysis used Shapiro-Wilk, Kruskal-Wallis, and Dunnett tests. Two-way ANOVA, Tukey, and Dunnett tests were applied for hDP metabolism and cytokine release. RESULTS: CH 2 % and HAp 5 % significantly enhanced GIC antimicrobial activity, especially after seven days. In immediate analysis, all materials showed reduced mitochondrial activity compared to the control. After 24 h, CH demonstrated mitochondrial metabolism similar to the control. All groups exhibited mild cytotoxicity (∼30 % cell death). Only IL-6 was influenced, with reduced release in experimental groups. SIGNIFICANCE: CH 2 % and HAp 5 % were most effective for antibacterial effects. GIC-CH 2 % emerged as the most promising formula, displaying significant antibacterial effects with reduced hDPSC toxicity.

Plant extract incorporated into glass ionomer cement as a photosensitizing agent for antimicrobial photodynamic therapy on Streptococcus mutans.

BACKGROUND: A plant extract (EB) incorporated into glass ionomer cement (GIG) could be a potential photosensitizer for Antimicrobial PDT (aPDT) against caries-microorganisms, replacing methylene blue (MB), due to the presence of chlorophyll. GIC + EB + aPDT could be an therapeutic alternative to dentin decontamination and sealing, allowing reduction of operative time. OBJECTIVE: Evaluate Dioscorea altissima (EB) incorporated into GIC as a photosensitizer for aPDT against Streptococcus mutans. METHODS: Groups (n = 24; ntotal = 192): G1-GIC; G2-GIC + LASER; G3-GIC/EB; G4-GIC/EB + LASER; G5-GIC+MB; G6-GIC + aPDT; G7-GIC/EB + MB; and G8 - GIC/EB+aPDT. In aPDT groups, MB was the photosensitizer. In LASER groups, MB was not used. The irradiation protocol was 660 nm/100 mW/5 J/150 J/cm²/50 s, with a 5-min pre-irradiation time for the MB groups. Antibacterial assays were carried out in 24-well microplates. The wells were completed with one milliliter of a S. mutans in BHI at 1.3 × 108 CFU/mL suspension. After incubation, PDT or laser was performed. After MTT bacteria viability test, the data were submitted to the Kolgomorov-Smirnoff normality test, followed by one-way ANOVA and Tukey's posterior test, α < 0.05. RESULTS: Group G6 showed significant inhibition (p < 0.001), followed by groups G4, G5, G7, and G8, which did not show significant differences among them (p > 0.05). Groups G2 and G3 also showed similar results (p > 0.05) and were the least active compared to the others. CONCLUSIONS: EB potentiated the antimicrobial action of GIC against S. mutans and laser irradiation over GIC/EB presented better antimicrobials results. The results indicate that EB could be a potential photosensitizer for aPDT.

Desafios atuais na conduta clínica em pacientes com Hipertensão Arterial Sistêmica ­ HAS / Urrent challenges in the clinical management of patients with Systemic Arterial Hypertension - SAH

INTRODUÇÃO: A hipertensão arterial sistêmica (HAS) é uma doença crônica, geralmente silenciosa e de alta prevalência. O cirurgião-dentista (CD) tem papel importante no acompanhamento e detecção de HAS subdiagnosticada. Além disso, a realização de procedimentos odontológicos em pacientes hipertensos, gera diversas dúvidas quanto à conduta, principalmente relacionadas com o limiar de pressão arterial (PA) e complicações que possam surgir durante o atendimento. OBJETIVO: Descrever estratégias de conduta e intervenção durante o atendimento odontológico de pacientes com HAS. METODOLOGIA: Foi realizada uma revisão integrativa por meio da busca nas bases de dados PUBMED/MEDLINE®, SCOPUS® e Web of Science com os seguintes descritores, "Hypertension AND Dental care AND Oral health". Foram incluídos os artigos que abordassem conduta odontológica em paciente com HAS, estivessem em inglês, disponíveis on-line e sem restrição de período. RESULTADOS: Foram encontrados 570 artigos que, depois da aplicação dos critérios de inclusão e exclusão, resultaram em sete artigos para leitura de texto completo. Verificou-se que todos eles tinham origem nos Estados Unidos da América, sendo que seis estudos eram revisões. CONCLUSÃO: Os estudos mostraram que não há necessidade de suspender os procedimentos odontológicos em pacientes assintomáticos com PA abaixo de 180-110 mmHg. Entretanto, sempre é preciso avaliar o perfil de risco, a presença de sintomas cardiovasculares, a extensão do procedimento odontológico, a necessidade de protocolo de redução de ansiedade e considerar o risco e benefício da intervenção.