Disentangling the dynamic interplay between muscle damage and energetics in male boxers during a short training block.

Boxing is a combat sport linked to muscle damage (e.g., soreness, rising creatine kinase [CK]) and energetic biomarkers (e.g., urea, glucose). These factors have not, however, been examined dynamically in terms of day-to-day, lagged and reciprocal effects during normal training. This study investigated the dynamic interplay between muscle damage and energetics in male boxers during a short training block. Thirteen amateur boxers were monitored over 16 consecutive days during early-season training. The participants were assessed each morning for plasma CK, urea, glucose, and creatinine (days 1 and 16 only) concentrations, before self-reporting muscle soreness (1-10 scale). Within-person contemporaneous (lag-0) and temporal (lag-1) networks were estimated using multilevel vector autoregression. Muscle soreness, CK, urea, and glucose presented different trajectories with training, but with some heterogeneity reflecting within-person variances (47% to 78%). The contemporaneous network yielded a significant positive edge (or correlation) between CK and soreness (r = 0.44), along with negative CK-glucose and glucose-urea edges. More significant edges emerged in the temporal network, with soreness linked to CK (r = 0.19), glucose (r = -0.28) and urea (r = 0.22), whilst the CK-glucose edge sign switched. In summary, daily fluctuations in muscle damage and energetic activity, which presented in a normal physiological range, were highly variable among boxers during early-season training. Within-person networks indicated some interrelatedness between CK, soreness, urea, and glucose, although the nature and presence of these relationships were contingent on temporal ordering. These inconsistences reflect the pleiotropy of energetic biomarkers in training and recovery.

Impact of one HF-rTMS session over the DLPFC and motor cortex on acute hormone dynamics and emotional state in healthy adults: a sham-controlled pilot study.

Studies indicate that high-frequency repetitive transcranial magnetic stimulation (HF-rTMS) can lower cortisol concentration or output, with some evidence suggesting a link to testosterone. Together, these stress and social hormones might help regulate the emotional response to HF-rTMS. This pilot study evaluated the effect of HF-rTMS on acute testosterone and cortisol dynamics and emotional state in eleven healthy adults. Using a sham-controlled, single-blind, crossover design, participants completed a HF-rTMS session targeting the dorsolateral prefrontal cortex (DLPFC) and motor cortex on separate days. Stimulation (250 total pulses) was applied at 90% of the resting motor threshold. Salivary testosterone and cortisol, mood, motivation, anxiety, and heart rate (HR) were assessed before (T1) and 1 (T2), 15 (T3), and 30 min (T4) after each session. There were no significant session differences in testosterone and cortisol concentration, mood, motivation, and HR. Although DLPFC stimulation produced less anxiety (vs. motor cortex), and testosterone output was stable across both treatments (vs. sham-related decline in testosterone), neither differed from the sham. Within-person fluctuations in testosterone, mood, motivation, and/or anxiety were significantly related across the DLPFC and motor cortex trials only. In conclusion, a single sub-maximal session of HF-rTMS did not affect the hormonal, emotional, or physiological state of healthy adults, relative to a sham. However, the emergence of stimulation-specific testosterone and/or emotional linkages suggests that the repeated effects of HF-rTMS may also manifest at the individual level. This offers another pathway to explain the therapeutic efficacy of rTMS and a model to explore interindividual variability in health-related outcomes.

Menstrual variation in the acute testosterone and cortisol response to laboratory stressors correlate with baseline testosterone fluctuations at a within- and between-person level.

Studies have compared HPA and HPG stress reactivity across the follicular and luteal phases to assess the menstrual impact of estradiol and progesterone fluctuations. Ovulatory shifts in baseline and stressor-induced testosterone among athletic women offer a new framework to explore these responses. Here we investigated menstrual variation in baseline testosterone as a predictor of the acute testosterone and cortisol response to laboratory stressors in female athletes. Using a semi-randomized crossover design, thirty athletic women completed a physical (4 × 6-s bike sprints) and psychological (5 × 2-min cognitive tests with social evaluation) stressor on day seven (D7), 14 (D14), and 21 (D21) of a menstrual cycle. Baseline fluctuations and acute changes in salivary testosterone and cortisol were measured. The D14 testosterone response to both stressors (13.7%) exceeded D7 (7.3%) and D21 (7.0%), whereas cortisol was less responsive on D14 (9.8%) than D7 (13.0%) and D21 (12.0%); all moderate to large effect size differences (p < 0.01). Baseline testosterone, which presented large individual and menstrual variation with a D14 peak, was significantly related (moderate correlations) to testosterone and cortisol stress reactivity on a between-person level. Both outcomes were related (weak correlations) to within-person fluctuations in baseline testosterone, but these effects were mediated by testing day. In conclusion, menstrual variation in baseline testosterone concentration correlated with testosterone and cortisol reactivity to a physical and psychological stressor. Thus, gradients of stressor-induced hormonal change showed some dependency to endogenous testosterone, both individual differences and fluctuations over time, among naturally cycling athletic women.Lay summaryThe female menstrual cycle is accompanied by dramatic shifts in estradiol and progesterone concentration, but less is known about testosterone variability and its role in stress regulation. In this study, menstrual fluctuations in baseline testosterone concentration correlated with acute testosterone and cortisol reactivity to laboratory stressors.

Testosterone and cortisol are more predictive of choice behavior than a social nudge in adult males on a simple gift give-get task.

Reproducibility of social research is ambitious, and evidence supporting this argument is increasing in psychology and social science research. This may be attributed to, in part, the high volume of qualitative research methodology used in social research along with difficulties in the reliability of measurement techniques. Therefore, use of more and better objective measures to complement existing techniques in social research are necessitated. To highlight this point we explored the success of give-get nudge in adults. Nudge being a subtle intervention to influence choice, without restricting choice. We also wanted to explore whether testosterone and cortisol, as objective psychophysiological markers, could explain nudge outcome. Participants were asked what they would like to get for Christmas, or what they would like to give. They were then presented with two chocolates, one big and one small, and instructed to take as a "reward" for their participation with the knowledge there was one other participant to take chocolate after them. It was hypothesized that those asked to give something for Christmas would take the smaller reward and vice versa. Salivary testosterone and cortisol were measured prior to, and 10 min after completing the exercise. The nature of the nudge itself did not predict behavior, but the hormone measures did. We speculate that testosterone may focus an individual on the nature of the question (nudge), while cortisol encourages self-focus. These results support the need to combine existing social research techniques with more objective markers.

Effect of Ischemic Preconditioning on Maximal Swimming Performance.

ABSTRACT: Williams, N, Russell, M, Cook, CJ, and Kilduff, LP. Effect of ischemic preconditioning on maximal swimming performance. J Strength Cond Res 35(1): 221-226, 2021-The effect of ischemic preconditioning (IPC) on swimming performance was examined. Using a randomized, crossover design, national- and international-level swimmers (n = 20; 14 men, 6 women) participated in 3 trials (Con, IPC-2h, and IPC-24h). Lower-body IPC (4 × 5-minute bilateral blood flow restriction at 160-228 mm Hg and 5-minute reperfusion) was used 2 hours (IPC-2h) or 24 hours (IPC-24h) before a self-selected (100 m, n = 15; 200 m, n = 5) swimming time trial (TT). The Con trial used a sham intervention (15 mm Hg) 2 hours before exercise. All trials required a 40-minute standardized precompetition swimming warm-up (followed by 20-minute rest; replicating precompetition call room procedures) 1 hour before TT. Capillary blood (pH, blood gases, and lactate concentrations) was taken immediately before and after IPC, before TT and after TT. No effects on TT for 100 m (P = 0.995; IPC-2h: 64.94 ± 8.33 seconds; IPC-24h: 64.67 ± 8.50 seconds; Con: 64.94 ± 8.24 seconds), 200 m (P = 0.405; IPC-2h: 127.70 ± 10.66 seconds; IPC-24h: 129.26 ± 12.99 seconds; Con: 130.19 ± 10.27 seconds), or combined total time (IPC-2h: 84.27 ± 31.52 seconds; IPC-24h: 79.87 ± 29.72 seconds; Con: 80.55 ± 31.35 seconds) were observed after IPC. Base excess (IPC-2h: -13.37 ± 8.90 mmol·L-1; Con: -13.35 ± 7.07 mmol·L-1; IPC-24h: -16.53 ± 4.65 mmol·L-1), pH (0.22 ± 0.08; all conditions), bicarbonate (IPC-2h: -11.66 ± 3.52 mmol·L-1; Con: -11.62 ± 5.59 mmol·L-1; IPC-24h: -8.47 ± 9.02 mmol·L-1), total carbon dioxide (IPC-2h: -12.90 ± 3.92 mmol·L-1; Con: -11.55 ± 7.61 mmol·L-1; IPC-24h: 9.90 ± 8.40 mmol·L-1), percentage oxygen saturation (IPC-2h: -0.16 ± 1.86%; Con: +0.20 ± 1.93%; IPC-24h: +0.47 ± 2.10%), and blood lactate (IPC-2h: +12.87 ± 3.62 mmol·L-1; Con: +12.41 ± 4.02 mmol·L-1; IPC-24h: +13.27 ± 3.81 mmol·L-1) were influenced by swimming TT (P < 0.001), but not condition (all P > 0.05). No effect of IPC was seen when applied 2 or 24 hours before swimming TT on any indices of performance or physiological measures recorded.

A longitudinal investigation of bidirectional and time-dependent interrelationships between testosterone and training motivation in an elite rugby environment.

In sport, testosterone has been positioned as a substrate for motivation with both directional and time dependencies. However, evidence is scarce when considering the complexities of competitive sport and no work has explicitly modeled these dependencies. To address these gaps, we investigated the bidirectional and time-dependent interrelationships between testosterone and training motivation in an elite rugby environment. Thirty-six male athletes were monitored across training weeks before and after eight international rugby matches. Pre-breakfast measures of salivary testosterone and training motivation (1-10 rating) were taken on training, competition, and recovery days (up to 40 tests). Using a continuous-time (CT) model, within-person estimates of autoregressive effects (persistence) and cross-lagged effects (relationships) were derived. A stronger, more persistent temporal association was identified for testosterone than for motivation. Cross-lagged effects verified that training motivation was positively related to testosterone at latter time points (p < 0.001). Discrete-time analyses revealed a non-linear association; increasing in strength from a zero-time lag to peak after 2.83 days (standardized effect = 0.25), before dissipation over longer lagged intervals. The testosterone relationship with ensuing training motivation was also positive, but non-significant. Match effects also appeared (p < 0.001) with a predicted decline in training motivation, but a rise in testosterone, at match onset. In summary, a positive association emerged between within-person fluctuations in self-appraised motivation to train and testosterone concentration in an elite rugby environment. The lagged, non-linear nature of this relationship and match predictions on both outcomes support, and extend, theoretical models linking testosterone and competitive behaviors.

Physiological and Performance Effects of Caffeine Gum Consumed During a Simulated Half-Time by Professional Academy Rugby Union Players.

Russell, M, Reynolds, NA, Crewther, BT, Cook, CJ, and Kilduff, L. Physiological and performance effects of caffeine gum consumed during a simulated half-time by professional academy rugby union players. J Strength Cond Res 34(1): 145-151, 2020-Despite the prevalence of caffeine (CAF) as an ergogenic aid, few studies have examined the use of caffeinated gums, especially during half-time in team sports. The physiological (blood lactate and salivary hormone concentrations) and performance (repeated sprints and cognitive function) effects of consuming CAF gum during a simulated half-time were examined. Professional academy rugby union players (n = 14) completed this double-blind, randomized, counterbalanced study. After pre-exercise measurements, players chewed a placebo (PLC) gum for 5 minutes before a standardized warm-up and completing repeated sprint testing (RSSA1). Thereafter, during a 15-minute simulated half-time period, players chewed either CAF (400 mg; 4.1 ± 0.5 mg·kg) or PLC gum for 5 minutes before completing a second repeated sprint test (RSSA2). Blood lactate, salivary testosterone and cortisol concentrations, and indices of cognitive function (i.e., reaction time and Stroop test) were measured at baseline, pre-RSSA1, post-RSSA1, pre-RSSA2, and post-RSSA2. Sprint performance was not affected by CAF (p = 0.995) despite slower sprint times after the first sprint of both RSSA tests (all p < 0.002). After half-time, salivary testosterone increased by 70% (+97 ± 58 pg·ml) in CAF vs. PLC (p < 0.001), whereas salivary cortisol remained unchanged (p = 0.307). Cognitive performance was unaffected by time and trial (all p > 0.05). Although performance effects were absent, chewing CAF gum increased the salivary testosterone concentrations of professional rugby union players over a simulated half-time. Practitioners may, therefore, choose to recommend CAF gum between successive exercise bouts because of the increases in salivary testosterone observed; a variable associated with increased motivation and high-intensity exercise performance.

Within- and between-person variation in morning testosterone is associated with economic risk-related decisions in athletic women across the menstrual cycle.

Literature suggests that women experience ovulatory shifts in risk-taking behaviours across different domains, which might be partly attributed to changes in testosterone (T). Thus, we investigated associations between menstrual variability in T concentrations and economic risk-related decisions among athletic women. Thirty-five women were monitored across three consecutive menstrual cycles. Testing occurred on day seven (D7), 14 (D14) and 21 (D21) following the onset of menses. The morning (7 to 8 am) assessment of salivary T (sal-T) and cortisol (sal-C) was followed by the economic Hawk-Dove game (11 am to 12 pm) played in pairs, where hawk decisions were used to index risk. Morning sal-T concentration increased from D7 to D14, before decreasing on D21 (p < 0.001), representing moderate effect size (ES) changes of 0.6 to 0.8. Morning sal-C did not vary over time. Hawk choices paralleled the sal-T results, being elevated on D14 (p < 0.001) with large ES changes of 1.8. Regression analyses revealed that morning sal-T concentration was positively related (p ≤ 0.01) to the number of hawks chosen between- (beta = 0.47) and within-participants (beta = 0.10) when controlling for training hours and menstrual day. In summary, the risk-related choices of athletic women during a dyadic contest covaried with morning sal-T concentrations across the menstrual cycle. Both outcomes were positively correlated on a within- and between-person level. Confirming the major sources of T variation across the menstrual cycle, whilst discerning its relationship with other risk-related behaviours, would be worthwhile avenues for research.

Neuromuscular, physiological and perceptual responses to an elite netball tournament.

To examine responses to an International netball tournament, female athletes (n= 11) played three matches over consecutive days. External (accelerometry) and internal (heart rate; HR, session; sRPE, and differential; dRPE, rating of perceived exertion) load measures quantified match intensity. On match-day mornings, and three days after match 3, well-being (brief assessment of mood; BAM+), biochemical (creatine kinase concentration; CK), neuromuscular (jump height; JH, peak power output; PPO) and endocrine function (salivary cortisol; C, testosterone; T, concentrations) were assessed. External load was similar between matches whereas dRPE and sRPE were greatest for match 3. Following match 1, CK increased, whereas BAM+, JH, C and T decreased. Following two matches, BAM+, PPO, and T decreased with CK increasing versus baseline. Following consecutive matches, CK (likely moderate; 27.9% ± 19.5%) and C (possibly moderate; 43.3% ± 46.8%) increased, whilst BAM+ (possibly moderate; -20.6% ± 24.4%) decreased. Three days post-tournament BAM+, T, PPO, and JH decreased. Mid-court elicited higher mean HR (possibly moderate; 3.7% ± 3.8%), internal and external intensities (possibly very large; 85.7% ± 49.6%) compared with goal-based positions. Consecutive matches revealed a dose-response relationship for well-being and physiological function; a response evident three days post-tournament.

Predictors of Linear and Multidirectional Acceleration in Elite Soccer Players.

Jonathan, N, Russell, M, Shearer, D, Cook, CJ, and Kilduff, LP. Predictors of linear and multidirectional acceleration in elite soccer players. J Strength Cond Res 33(2): 514-522, 2019-Linear and multidirectional acceleration underpins success in professional soccer match play. However, the physical qualities that determine these performance indicators are poorly understood in elite players. English Premier League players (n = 26) performed isometric midthigh pulls (IMTPs), bilateral and unilateral drop jumps (from 40 and 20 cm, respectively), bilateral and unilateral countermovement jumps (CMJs), and assessments of linear (5, 10, and 20 m) and multidirectional (left and right preplanned and reactive) acceleration. Regression analyses highlighted that 21% of variance in 5-m sprint time (1.02 ± 0.07 seconds) was explained by relative peak power output (PPO) in bilateral CMJ (54.5 ± 5.3 W·kg). A 5.4 W·kg increase in CMJ predicted a 0.03-second decrease in 5-m sprint time (P = 0.02). For 10-m sprint time (1.72 ± 0.09 seconds), 44% of variance was explained by isometric relative peak force ([PF]; 30.4 ± 4.9 N·kg) and bilateral relative CMJ PPO (54.5 ± 5.3 W·kg). A 5.4 W·kg increase in CMJ predicted reduced 10-m sprint times by 0.04 seconds (P = 0.01). For 20-m sprint time (2.94 ± 0.11 seconds), 55% of the total variance was explained by isometric relative PF (30.4 ± 4.9 N·kg) and relative CMJ PPO (54.5 ± 5.3 W·kg). Increases of 5.4 W·kg in bilateral CMJ predicted an improvement of 20-m sprint time by 0.06 seconds (P = 0.002). Contributions were insignificant (P > 0.05) for preplanned and reactive multidirectional acceleration. Relativized indices, especially those related to force production during CMJ and IMTP tests, likely underpin linear but not multidirectional acceleration performance in professional soccer players. When linear acceleration is a training focus, practitioners should seek to monitor CMJ and IMTP test performance.

The salivary testosterone response to a chance-determined contest is associated with face-gazing behaviours in athletic women.

Human gaze is an important indicator of dominant and submissive behaviours related to positioning in a social hierarchy. This study investigated face gazing after a chance-determined contest and its linkage to salivary testosterone (sal-T) reactivity in athletic women. Twenty-six women athletes completed a coin-toss game on days 7 (D7), 14 (D14) and 21 (D21) of a single menstrual cycle. The game was played against an unknown opponent of similar age with the winner congratulated and rewarded with all coins. Gazing towards an opponent's head or face was timed after each contest (over 2 min) from video footage. Salivary T (sal-T) was assessed before and 15 min after these contests. The sal-T residuals increased after winning and decreased after losing on D7, D14 and D21 (p < 0.05). Gaze times were longer after a loss (M = 7.8 s) than a win (M = 3.1 s) across all days. Regression analyses revealed that the sal-T residuals and contest outcome interacted to predict gaze time. Upon deconstruction we found that, when losing a contest, a larger sal-T response (i.e., smaller decline) predicted a longer gaze (ß = 1.71, p = 0.004), but no association was evident when winning (ß = -0.06, p = 0.821), and these slope patterns differed (p = 0.011). In conclusion, winning a contest by chance increased sal-T responsivity and decreased opponent gaze across the menstrual cycle. A positive relationship between individual sal-T reactivity and gaze duration was observed, but only when losing. These preliminary results support suggestions that women's T may help modulate post-competition behaviours (e.g., face gazing) possibly to achieve social cohesion and cooperation.

Serum cortisol as a moderator of the relationship between serum testosterone and Olympic weightlifting performance in real and simulated competitions.

Some equivocality exists regarding the effect of testosterone (T) on competitive performance with cortisol (C) implicated as a moderating factor. This study investigated whether C is moderating the T relationship with Olympic weightlifting (OWL) performance during real and simulated competitions. We monitored 105 male athletes (age 19.3±3.7 years); 46 during real OWL competitions (e.g., national age championships) and 59 across simulated events (e.g., talent identification). Serum T and C were assessed before warming up and within 15 minutes of event completion. Performance was indexed by the total combined load across the snatch and clean and jerk exercises. Hierarchical linear regression and simple slopes were employed to test the hormone and performance relationships. Pre-competition T (pre-T) and C (pre-C) were unrelated to OWL performance when controlling for competition type, time of day, age, and body mass (model=75.6% variance). However, the pre-T × pre-C interaction was significant (model=77% variance). Upon exploring this interaction, different pre-T and performance relationships emerged for males with high pre-C (ß=-9.96) and low pre-C levels (ß=9.04), with diverging slopes (p=0.006). The assessment of T changes and pre-C produced similar results. The association between male T and performance during OWL competition was determined by C activity, which could explain conflicting reports of T as a correlate of competitive abilities. Our results imply that T and C are not strictly anabolic and catabolic biomarkers of performance, respectively, but rather they exert complementary actions that could depend on task, situational and environmental needs.

COL5A1 gene variants previously associated with reduced soft tissue injury risk are associated with elite athlete status in rugby.

BACKGROUND: Two common single nucleotide polymorphisms within the COL5A1 gene (SNPs; rs12722 C/T and rs3196378 C/A) have previously been associated with tendon and ligament pathologies. Given the high incidence of tendon and ligament injuries in elite rugby athletes, we hypothesised that both SNPs would be associated with career success. RESULTS: In 1105 participants (RugbyGene project), comprising 460 elite rugby union (RU), 88 elite rugby league athletes and 565 non-athlete controls, DNA was collected and genotyped for the COL5A1 rs12722 and rs3196378 variants using real-time PCR. For rs12722, the injury-protective CC genotype and C allele were more common in all athletes (21% and 47%, respectively) and RU athletes (22% and 48%) than in controls (16% and 41%, P ≤ 0.01). For rs3196378, the CC genotype and C allele were overrepresented in all athletes (23% and 48%) and RU athletes (24% and 49%) compared with controls (16% and 41%, P ≤ 0.02). The CC genotype in particular was overrepresented in the back and centres (24%) compared with controls, with more than twice the odds (OR = 2.25, P = 0.006) of possessing the injury-protective CC genotype. Furthermore, when considering both SNPs simultaneously, the CC-CC SNP-SNP combination and C-C inferred allele combination were higher in all the athlete groups (≥18% and ≥43%) compared with controls (13% and 40%; P = 0.01). However, no genotype differences were identified for either SNP when RU playing positions were compared directly with each other. CONCLUSION: It appears that the C alleles, CC genotypes and resulting combinations of both rs12722 and rs3196378 are beneficial for rugby athletes to achieve elite status and carriage of these variants may impart an inherited resistance against soft tissue injury, despite exposure to the high-risk environment of elite rugby. These data have implications for the management of inter-individual differences in injury risk amongst elite athletes.

Managing player load in professional rugby union: a review of current knowledge and practices.

BACKGROUND: The loads to which professional rugby players are subjected has been identified as a concern by coaches, players and administrators. In November 2014, World Rugby commissioned an expert group to identify the physical demands and non-physical load issues associated with participation in professional rugby. OBJECTIVE: To describe the current state of knowledge about the loads encountered by professional rugby players and the implications for their physical and mental health. FINDINGS: The group defined 'load' as it relates to professional rugby players as the total stressors and demands applied to the players. In the 2013-2014 seasons, 40% of professional players appeared in 20 matches or more, and 5% of players appeared in 30 matches or more. Matches account for ∼5-11% of exposure to rugby-related activities (matches, team and individual training sessions) during professional competitions. The match injury rate is about 27 times higher than that in training. The working group surmised that players entering a new level of play, players with unresolved previous injuries, players who are relatively older and players who are subjected to rapid increases in load are probably at increased risk of injury. A mix of 'objective' and 'subjective' measures in conjunction with effective communication among team staff and between staff and players was held to be the best approach to monitoring and managing player loads. While comprehensive monitoring holds promise for individually addressing player loads, it brings with it ethical and legal responsibilities that rugby organisations need to address to ensure that players' personal information is adequately protected. CONCLUSIONS: Administrators, broadcasters, team owners, team staff and the players themselves have important roles in balancing the desire to have the 'best players' on the field with the ongoing health of players. In contrast, the coaching, fitness and medical staff exert significant control over the activities, duration and intensity of training sessions. If load is a major risk factor for injury, then managing training loads should be an important element in enabling players to perform in a fit state as often as possible.

The Effects of a Single Whole-Body Cryotherapy Exposure on Physiological, Performance, and Perceptual Responses of Professional Academy Soccer Players After Repeated Sprint Exercise.

Russell, M, Birch, J, Love, T, Cook, CJ, Bracken, RM, Taylor, T, Swift, E, Cockburn, E, Finn, C, Cunningham, D, Wilson, L, and Kilduff, LP. The effects of a single whole-body cryotherapy exposure on physiological, performance, and perceptual responses of professional academy soccer players after repeated sprint exercise. J Strength Cond Res 31(2): 415-421, 2017-In professional youth soccer players, the physiological, performance, and perceptual effects of a single whole-body cryotherapy (WBC) session performed shortly after repeated sprint exercise were investigated. In a randomized, counterbalanced, and crossover design, 14 habituated English Premier League academy soccer players performed 15 × 30 m sprints (each followed by a 10 m forced deceleration) on 2 occasions. Within 20 minutes of exercise cessation, players entered a WBC chamber (Cryo: 30 seconds at -60° C, 120 seconds at -135° C) or remained seated (Con) indoors in temperate conditions (∼25° C). Blood and saliva samples, peak power output (countermovement jump), and perceptual indices of recovery and soreness were assessed pre-exercise and immediately, 2-hour and 24-hour postexercise. When compared with Con, a greater testosterone response was observed at 2-hour (+32.5 ± 32.3 pg·ml, +21%) and 24-hour (+50.4 ± 48.9 pg·ml, +28%) postexercise (both P = 0.002) in Cryo (trial × treatment interaction: P = 0.001). No between-trial differences were observed for other salivary (cortisol and testosterone/cortisol ratio), blood (lactate and creatine kinase), performance (peak power output), or perceptual (recovery or soreness) markers (all trial × treatment interactions: P > 0.05); all of which were influenced by exercise (time effects: all P ≤ 0.05). A single session of WBC performed within 20 minutes of repeated sprint exercise elevated testosterone concentrations for 24 hours but did not affect any other performance, physiological, or perceptual measurements taken. Although unclear, WBC may be efficacious for professional soccer players during congested fixture periods.

Skill acquisition and stress adaptations following laparoscopic surgery training and detraining in novice surgeons.

BACKGROUND: Surgical training and practice is stressful, but adaptive changes in the stress circuitry (e.g. perceptual, physiological, hormonal, neural) could support skill development. This work examined skill acquisition and stress adaptations in novice surgeons during laparoscopic surgery (LS) training and detraining. METHODS: Twelve medical students were assessed for skill performance after 2 h (BASE), 5 h (MID) and 8 h (POST) of LS training in weeks 1-3, and then after 4 weeks of no training (RETEST). The stress outcomes included state anxiety, perceived stress and workload, heart rate (HR), heart rate variability (HRV), and salivary testosterone and cortisol concentrations. Functional near-infrared spectroscopy was used to assess cortical oxygenation change, as a marker of prefrontal cortex (PFC) activity. RESULTS: Skill performance improved in every session from BASE (p < 0.01), with corresponding decreases in state anxiety, stress, workload, low- and high-frequency HRV in the MID, POST and/or RETEST sessions (p < 0.05). Left and right PFC were symmetrically activated within each testing session (p < 0.01). The stress and workload measures predicted skill performance and changes over time (p < 0.05), with state anxiety, mean HR and the HRV measures also showing some predictive potential (p < 0.10). CONCLUSIONS: A 3-week LS training programme promoted stress-related adaptations likely to directly, or indirectly, support the acquisition of new surgical skills, and many outcomes were retained after a 4-week period without further LS training. These results have implications for medical training and education (e.g. distributed training for skill development and maintenance, stress resource and management training) and highlighted possible areas for new research (e.g. longitudinal stress and skill profiling).

Post-warmup strategies to maintain body temperature and physical performance in professional rugby union players.

We compared the effects of using passive-heat maintenance, explosive activity or a combination of both strategies during the post-warmup recovery time on physical performance. After a standardised warmup, 16 professional rugby union players, in a randomised design, completed a counter-movement jump (peak power output) before resting for 20 min and wearing normal-training attire (CON), wearing a passive heat maintenance (PHM) jacket, wearing normal attire and performing 3 × 5 CMJ (with a 20% body mass load) after 12 min of recovery (neuromuscular function, NMF), or combining PHM and NMF (COMB). After 20 min, participants completed further counter-movement jump and a repeated sprint protocol. Core temperature (Tcore) was measured at baseline, post-warmup and post-20 min. After 20 min of recovery, Tcore was significantly lower under CON and NMF, when compared with both PHM and COMB (P < 0.05); PHM and COMB were similar. Peak power output had declined from post-warmup under all conditions (P < 0.001); however, the drop was less in COMB versus all other conditions (P < 0.05). Repeated sprint performance was significantly better under COMB when compared to all other conditions. Combining PHM with NMF priming attenuates the post-warmup decline in Tcore and can positively influence physical performance in professional rugby union players.

Abbreviated Resonant Frequency Training to Augment Heart Rate Variability and Enhance On-Demand Emotional Regulation in Elite Sport Support Staff.

Support and management staff in elite sport experience work-related stress and emotional disturbance to a similar extent as athletes (Fletcher and Wagstaff 2009). The resonant frequency breathing technique (Lehrer et al. 2000) can inhibit autonomic changes associated with stressful situations or events and as such provides a potential emotional regulation tool. The present study utilised five practitioner-led heart rate variability (HRV) biofeedback sessions and home practice via mobile applications to train support and management staff (n = 9) in resonant frequency breathing techniques. Although baseline HRV did not change from pre to post training, participants increased total HRV (i.e., SDNN; p = .006), parasympathetic HRV (i.e., RMSSD; p = .028) and HRV reflective of baroreflex function (i.e., low frequency power; p = .018) while accurately performing resonant frequency breathing without a breath pacer. Post-intervention questionnaire data revealed an increase (p = .032) in habitual use of somatic strategies for emotional regulation, and social validation data suggested that the technique enhanced emotional regulation at home, work and during international competition. HRV biofeedback and the resonant frequency technique provided an on-demand emotional regulation technique for elite sport support and management staff.

The Neuromuscular, Biochemical, and Endocrine Responses to a Single-Session Vs. Double-Session Training Day in Elite Athletes.

Johnston, MJ, Cook, CJ, Drake, D, Costley, L, Johnston, JP, and Kilduff, LP. The neuromuscular, biochemical, and endocrine responses to a single-session vs. double-session training day in elite athletes. J Strength Cond Res 30(11): 3098-3106, 2016-The aim of this study was to compare the acute neuromuscular, biochemical, and endocrine responses of a training day consisting of a speed session only with performing a speed-and-weights training session on the same day. Fifteen men who were academy-level rugby players completed 2 protocols in a randomized order. The speed-only protocol involved performing 6 maximal effort repetitions of 50-m running sprints with 5 minutes of recovery between each sprint, whereas the speed-and-weights protocol involved the same sprinting session but was followed 2 hours later by a lower-body weights session consisting of 4 sets of 5 backsquats and Romanian deadlift at 85% one repetition maximum. Testosterone, cortisol, creatine kinase, lactate, and perceived muscle soreness were determined immediately before, immediately after, 2 hours after, and 24 hours after both the protocols. Peak power, relative peak power, jump height, and average rate of force development were determined from a countermovement jump (CMJ) at the same time points. After 24-hours, muscle soreness was significantly higher after the speed-and-weights protocol compared with the speed-only protocol (effect size η = 0.253, F = 4.750, p ≤ 0.05). There was no significant difference between any of the CMJ variables at any of the posttraining time points. Likewise, creatine kinase, testosterone, and cortisol were unaffected by the addition of a weight-training session. These data indicate that the addition of a weight-training session 2 hours after a speed session, whereas increasing the perception of fatigue the next day does not result in a difference in endocrine response or in neuromuscular capability.

Changes in Acceleration and Deceleration Capacity Throughout Professional Soccer Match-Play.

Russell, M, Sparkes, W, Northeast, J, Cook, CJ, Love, TD, Bracken, RM, and Kilduff, LP. Changes in acceleration and deceleration capacity throughout professional soccer match-play. J Strength Cond Res 30(10): 2839-2844, 2016-As the acceleration and deceleration demands of soccer are currently not well understood, this study aimed to profile markers of acceleration and deceleration capacity during professional soccer match-play. This within-player observational study required reserve team players from a Premier League club to wear 10-Hz Global Positioning System units throughout competitive matches played in the 2013-14 competitive season. Data are presented for players who completed 4 or more games during the season (n = 11), and variables are presented according to six 15-minute intervals (I1-6: 00:00-14:59 minutes, 15:00-29:59 minutes, 30:00-44:59 minutes, 45:00-59:59 minutes, 60:00-74:59 minutes, and 75:00-89:59 minutes, respectively). During I6, the distance covered (total, per minute, and at high intensity), number of sprints, accelerations (total and high intensity), decelerations (total and high intensity), and impacts were reduced compared with I1 (all p ≤ 0.05). The number of high-intensity impacts remained unchanged throughout match-play (p > 0.05). These findings indicate that high-intensity actions and markers of acceleration and deceleration capacity are reduced in the last 15 minutes of the normal duration of match-play. Such information can be used to increase the specificity of training programs designed for soccer players while also giving further insight in to the effects of 90 minutes of soccer-specific exercise. Interventions that seek to maintain the acceleration and deceleration capacity of players throughout the full duration of a soccer match warrant investigation.