ABSTRACT
Bromine is ubiquitously present in animals as ionic bromide (Br(-)) yet has no known essential function. Herein, we demonstrate that Br(-) is a required cofactor for peroxidasin-catalyzed formation of sulfilimine crosslinks, a posttranslational modification essential for tissue development and architecture found within the collagen IV scaffold of basement membranes (BMs). Bromide, converted to hypobromous acid, forms a bromosulfonium-ion intermediate that energetically selects for sulfilimine formation. Dietary Br deficiency is lethal in Drosophila, whereas Br replenishment restores viability, demonstrating its physiologic requirement. Importantly, Br-deficient flies phenocopy the developmental and BM defects observed in peroxidasin mutants and indicate a functional connection between Br(-), collagen IV, and peroxidasin. We establish that Br(-) is required for sulfilimine formation within collagen IV, an event critical for BM assembly and tissue development. Thus, bromine is an essential trace element for all animals, and its deficiency may be relevant to BM alterations observed in nutritional and smoking-related disease. PAPERFLICK:
Subject(s)
Basement Membrane/metabolism , Bromine/metabolism , Drosophila/growth & development , Trace Elements/metabolism , Animals , Basement Membrane/ultrastructure , Bromine/deficiency , Cell Line , Collagen/metabolism , Drosophila/metabolism , Extracellular Matrix Proteins/genetics , Extracellular Matrix Proteins/metabolism , Humans , Imines/metabolism , Larva/ultrastructure , Mice , Peroxidase/genetics , Peroxidase/metabolism , PeroxidasinABSTRACT
Collagen IV comprises the predominant protein network of basement membranes, a specialized extracellular matrix, which underlie epithelia and endothelia. These networks assemble through oligomerization and covalent crosslinking to endow mechanical strength and shape cell behavior through interactions with cell-surface receptors. A recently discovered sulfilimine (S=N) bond between a methionine sulfur and hydroxylysine nitrogen reinforces the collagen IV network. We demonstrate that peroxidasin, an enzyme found in basement membranes, catalyzes formation of the sulfilimine bond. Drosophila peroxidasin mutants have disorganized collagen IV networks and torn visceral muscle basement membranes, pointing to a critical role for the enzyme in tissue biogenesis. Peroxidasin generates hypohalous acids as reaction intermediates, suggesting a paradoxically anabolic role for these usually destructive oxidants. This work highlights sulfilimine bond formation as what is to our knowledge the first known physiologic function for peroxidasin, a role for hypohalous oxidants in tissue biogenesis, and a possible role for peroxidasin in inflammatory diseases.
Subject(s)
Acids/chemistry , Extracellular Matrix Proteins/chemistry , Imines/chemistry , Peroxidase/chemistry , Animals , Catalysis , Collagen Type IV/chemistry , Drosophila/chemistry , PeroxidasinABSTRACT
The study of collagen IV has benefited greatly from the seminal work conducted by Arthur Veis and colleagues over three decades ago. Through a series of electron microscopy studies focused on lens basement membrane, an appreciation was gained for the distinct network-forming properties of collagen IV. Veis correctly suggested that network assembly is a phenomenon of the non-collagenous termini of the molecule. This review seeks to document how the field advanced following these seminal conclusions, including recent discoveries regarding the molecular reinforcement of networks that support Veis' conclusions.
Subject(s)
Collagen Type IV/metabolism , Lens Capsule, Crystalline/metabolism , Animals , Basement Membrane/metabolism , Cells/metabolism , Collagen Type IV/chemistry , Humans , Models, BiologicalABSTRACT
Collagen IV scaffolds assemble through an intricate pathway that begins intracellularly and is completed extracellularly. Multiple intracellular enzymes act in concert to assemble collagen IV protomers, the building blocks of collagen IV scaffolds. After being secreted from cells, protomers are activated to initiate oligomerization, forming insoluble networks that are structurally reinforced with covalent crosslinks. Within these networks, embedded binding sites along the length of the protomer lead to the "decoration" of collagen IV triple helix with numerous functional molecules. We refer to these networks as "smart" scaffolds, which as a component of the basement membrane enable the development and function of multicellular tissues in all animal phyla. In this review, we present key molecular mechanisms that drive the assembly of collagen IV smart scaffolds.
Subject(s)
Amino Acid Oxidoreductases/genetics , Antigens, Neoplasm/genetics , Collagen Type IV/chemistry , Extracellular Matrix/metabolism , Protein Subunits/chemistry , Receptors, Interleukin-1/genetics , Amino Acid Motifs , Amino Acid Oxidoreductases/metabolism , Animals , Antigens, Neoplasm/metabolism , Basement Membrane/metabolism , Basement Membrane/ultrastructure , Collagen Type IV/genetics , Collagen Type IV/metabolism , Eukaryotic Cells/metabolism , Eukaryotic Cells/ultrastructure , Extracellular Matrix/ultrastructure , Gene Expression Regulation , Humans , Peroxidases , Protein Interaction Domains and Motifs , Protein Multimerization , Protein Structure, Secondary , Protein Subunits/genetics , Protein Subunits/metabolism , Receptors, Interleukin-1/metabolismABSTRACT
Basement membranes are defining features of the cellular microenvironment; however, little is known regarding their assembly outside cells. We report that extracellular Cl(-) ions signal the assembly of collagen IV networks outside cells by triggering a conformational switch within collagen IV noncollagenous 1 (NC1) domains. Depletion of Cl(-) in cell culture perturbed collagen IV networks, disrupted matrix architecture, and repositioned basement membrane proteins. Phylogenetic evidence indicates this conformational switch is a fundamental mechanism of collagen IV network assembly throughout Metazoa. Using recombinant triple helical protomers, we prove that NC1 domains direct both protomer and network assembly and show in Drosophila that NC1 architecture is critical for incorporation into basement membranes. These discoveries provide an atomic-level understanding of the dynamic interactions between extracellular Cl(-) and collagen IV assembly outside cells, a critical step in the assembly and organization of basement membranes that enable tissue architecture and function. Moreover, this provides a mechanistic framework for understanding the molecular pathobiology of NC1 domains.