The pH-responsive transcription factor PacC regulates mycelial growth, fruiting body development, and ganoderic acid biosynthesis in Ganoderma lucidum.

Ganoderma lucidum is a medicinal macrofungus that is widely used in traditional Chinese medicine. Nonetheless, the scarcity of basic biological studies of this organism has hindered the further development of its commercial value. The pH-responsive transcription factor PacC/Rim101 governs the adaptation to environmental pH, the development and the secondary metabolism of many fungi. In this study, a homologue of PacC/Rim101 that encodes GlPacC was identified in the higher basidiomycete G. lucidum. GlPacC is composed of 807 amino acids and contains three typical C2H2 zinc-finger domains, two potential PEST domains, a putative PKA phosphorylation site, and a putative nuclear localization signal (NLS). GlPacC was transcribed at a high level when the fungus was under neutral and alkaline conditions, and silencing of GlPacC impaired the fungal response to ambient pH. The distance between the hyphal branches (of vegetative hyphae and aerial hyphae) was significantly increased in the GlPacC-silenced strains. The GlPacC-silenced strains grew abnormally or became sickly on solid culture medium and were unable to form primordia and fruiting bodies. The ganoderic acid content, levels of the sqs and ls transcripts, and contents of the metabolic intermediates squalene and lanosterol were all up-regulated in the GlPacC-silenced strains. Our results indicate that GlPacC is functional and plays complex roles in mycelial growth, fruiting body development and ganoderic acid biosynthesis in G. lucidum.

Interactive Effects of [CO2] and Temperature on Plant Chemistry of Transgenic Bt Rice and Population Dynamics of a Non-Target Planthopper, Nilaparvata lugens (Stål) under Different Levels of Soil Nitrogen.

Gaining a better understanding of the interactive effect of projected atmospheric CO2 level increase and the Earth's rising temperature on plant chemistry (nutritional and defensive characteristics) of transgenic crops is essential when attempting to forecast the responses of target and non-target insects to climate change. In this study, effects of carbon dioxide (CO2; elevated versus ambient), temperature (T; high versus low), and their interactions on leaf nitrogen content (N%) and C:N ratio of transgenic Bt rice and its non-Bt isoline grown under low- and high-N fertilizer were systematically analyzed together with the resulting insect population dynamics of a non-target planthopper Nilaparvata lugens (Stâl) in open-top-chamber experiments. The results indicated that under low-N treatment, elevated CO2 at low T (i.e., eCO2) (compared to ambient CO2 at low T, i.e., CK) significantly decreased N% and Bt-toxin content and significantly increased C:N ratio in leaf sheath and leaf of Bt rice, especially during the tillering stage, whereas inverse effects of high T were shown on the plant chemistry of Bt rice, especially during heading stage. The combination of elevated CO2 and high T (i.e., Combined) (in contrast to CK) significantly increased N% and decreased C:N ratio in leaf sheath of Bt rice during the heading stage under low-N fertilizer, while significantly decreased N% and increased C:N ratio in leaf of Bt rice during the tillering stage, regardless of fertilizer-N level, and significantly increased Bt-toxin content in leaf sheath and leaf during the tillering stage under both low- and high-N. Moreover, no discernable relationships between Bt-toxin content and N% or leaf C:N ratio were observed at any CO2 or N levels evaluated. Furthermore, transgenic treatment, temperature and fertilizer-N level interactions, and CO2 and fertilizer-N level interactions all significantly affected the population dynamics of N. lugens. Specifically, high-N significantly enhanced the population dynamics of N. lugens fed on non-Bt rice grown under eTemp and Bt cultivar significantly reduced the population dynamics of N. lugens under eCO2 regardless of N fertilizer levels. The study demonstrates that the planting of transgenic Bt rice would not increase the risk of increased N. lugens severity under the combined condition of elevated CO2 and increased temperature, particularly under moderate level of N fertility.

Effects of Exogenous Salicylic Acid on Ganoderic Acid Biosynthesis and the Expression of Key Genes in the Ganoderic Acid Biosynthesis Pathway in the Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes).

We demonstrate herein that salicylic acid (SA) can enhance ganoderic acid (GA) accumulation in the lingzhi or reishi medicinal mushroom Ganoderma lucidum. Following treatment with different concentrations of SA, the GA content was increased 22.72% to 43.04% compared with the control group. When the fungi were treated with 200 µmol/L SA at different times, the GA content was improved 10.21% to 35.24% compared with the control group. By choosing the optimum point based on response surface methodology, the GA content could be increased up to 229.03 µg/100 mg, which was improved 66.38% compared with the control group. When the fungi were treated with 200 µmol/L SA, the transcription levels of key genes in the GA biosynthesis pathway-squalene (SQ) synthase (sqs), lanosterol (Lano; osc), and hydroxy-3-methylglutaryl-coenzyme A reductase (hmgr)-were improved 119.6-, 3.2-, and 4.2-fold, respectively. In addition, following treatment with 100 µmol/L SA, the levels of Lano and SQ, which are intermediate metabolites of GA biosynthesis, were increased 2.8- and 1.4-fold, respectively. These results indicate that SA can regulate the expression of genes related to GA biosynthesis and increases the metabolic levels of Lano and SQ, thereby resulting in the accumulation of GA.

Single and fused transgenic Bacillus thuringiensis rice alter the species-specific responses of non-target planthoppers to elevated carbon dioxide and temperature.

BACKGROUND: The approval of transgenic Bacillus thuringiensis (Bt) rice by China was momentous for biotech crops, although it has yet to be approved for commercial production. Non-target pest problems in rice paddies, such as the three ecologically similar species of planthoppers Nilaparvata lugens, Laodelphax striatellus and Sogatella furcifera, could become increasingly serious under global climate change. Fused (Cry1Ab/Cry1Ac) and single (Cry1Ab) transgenic Bt rice were evaluated for effects on species-specific responses of planthoppers to elevated carbon dioxide (CO2) and temperature. RESULTS: Transgenic Bt rice lines significantly modified species-specific responses of the planthoppers to elevated CO2 and temperature. High temperature appears to favour outbreaks of S. furcifera relative to N. lugens and L. striatellus when feeding upon fused transgenic Bt rice, especially at elevated CO2 . Elevated CO2 at high temperature appears to be a factor reducing S. furcifera occurrence when feeding upon single transgenic Bt rice. CONCLUSION: Different types of transgenic Bt rice alter the species-specific responses of non-target planthoppers to elevated CO2 and temperature. Compared with their non-transgenic parental lines, the single transgenic Bt rice shows better performance in controlling the non-target planthopper S. furcifera by comparison with the fused transgenic Bt rice under elevated CO2 and temperature. It is suggested that multitypes of transgenic Bt rice be used in the field simultaneously in order to take advantage of high transgenic diversity for optimal performance against all pests in paddy fields.