1.
Genet Mol Res
; 14(4): 18485-93, 2015 Dec 28.
Article
in English
| MEDLINE
| ID: mdl-26782496
ABSTRACT
In this study, Lactococcus lactis was engineered to express mutated internalin A on its surface and to secrete large amounts of listeriolysin O (LLO) in order to improve its potential as a vehicle for DNA vaccination. Western blotting experiments demonstrated that the bacterium expressed LLO in both the cytoplasmic and extracellular compartments, with higher quantities found in the culture supernatants. A hemolytic assay showed that the recombinant strain secreted 250 ng active LLO/mg total protein. This mInlA/LLO-producing strain of L. lactis may be used as an alternative tool in DNA vaccination against a number of infectious diseases or in cancer therapy.
Subject(s)
Bacterial Proteins/genetics , Bacterial Proteins/immunology , Bacterial Toxins/genetics , Bacterial Toxins/immunology , Heat-Shock Proteins/genetics , Heat-Shock Proteins/immunology , Hemolysin Proteins/genetics , Hemolysin Proteins/immunology , Lactococcus lactis/genetics , Listeria monocytogenes/immunology , Mutation , Recombinant Proteins , Bacterial Vaccines , Cell Membrane/metabolism , Gene Expression , Hemolysis , Lactococcus lactis/metabolism , Vaccination
2.
Rev Port Pediatr Pueric
; 30(12): 346-60, 1967 Dec.
Article
in Portuguese
| MEDLINE
| ID: mdl-5603025
Subject(s)
Carbohydrate Metabolism , Malabsorption Syndromes/physiopathology , Child , Child, Preschool , Female , Humans , Infant , Male
3.
Rev Port Pediatr Pueric
; 32(3): 58-69, 1969.
Article
in Portuguese
| MEDLINE
| ID: mdl-5810345
Subject(s)
Pediatrics/education , Adolescent , Adult , Africa , Aged , Asia , Australia , Central America , Child , Child, Preschool , Europe , Humans , Infant , Infant Mortality , Infant, Newborn , Middle Aged , North America , Portugal , South America
4.
Rev Port Pediatr Pueric
; 31(11): 261-9, 1968.
Article
in English
| MEDLINE
| ID: mdl-5733542