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1.
Epilepsia ; 65(6): 1568-1580, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38606600

ABSTRACT

OBJECTIVE: This study was undertaken to determine whether hippocampal T2 hyperintensity predicts sequelae of febrile status epilepticus, including hippocampal atrophy, sclerosis, and mesial temporal lobe epilepsy. METHODS: Acute magnetic resonance imaging (MRI) was obtained within a mean of 4.4 (SD = 5.5, median = 2.0) days after febrile status on >200 infants with follow-up MRI at approximately 1, 5, and 10 years. Hippocampal size, morphology, and T2 signal intensity were scored visually by neuroradiologists blinded to clinical details. Hippocampal volumetry provided quantitative measurement. Upon the occurrence of two or more unprovoked seizures, subjects were reassessed for epilepsy. Hippocampal volumes were normalized using total brain volumes. RESULTS: Fourteen of 22 subjects with acute hippocampal T2 hyperintensity returned for follow-up MRI, and 10 developed definite hippocampal sclerosis, which persisted through the 10-year follow-up. Hippocampi appearing normal initially remained normal on visual inspection. However, in subjects with normal-appearing hippocampi, volumetrics indicated that male, but not female, hippocampi were smaller than controls, but increasing hippocampal asymmetry was not seen following febrile status. Forty-four subjects developed epilepsy; six developed mesial temporal lobe epilepsy and, of the six, two had definite, two had equivocal, and two had no hippocampal sclerosis. Only one subject developed mesial temporal epilepsy without initial hyperintensity, and that subject had hippocampal malrotation. Ten-year cumulative incidence of all types of epilepsy, including mesial temporal epilepsy, was highest in subjects with initial T2 hyperintensity and lowest in those with normal signal and no other brain abnormalities. SIGNIFICANCE: Hippocampal T2 hyperintensity following febrile status epilepticus predicted hippocampal sclerosis and significant likelihood of mesial temporal lobe epilepsy. Normal hippocampal appearance in the acute postictal MRI was followed by maintained normal appearance, symmetric growth, and lower risk of epilepsy. Volumetric measurement detected mildly decreased hippocampal volume in males with febrile status.


Subject(s)
Epilepsy, Temporal Lobe , Hippocampus , Magnetic Resonance Imaging , Sclerosis , Seizures, Febrile , Status Epilepticus , Humans , Hippocampus/pathology , Hippocampus/diagnostic imaging , Epilepsy, Temporal Lobe/diagnostic imaging , Epilepsy, Temporal Lobe/pathology , Male , Female , Sclerosis/pathology , Status Epilepticus/diagnostic imaging , Status Epilepticus/pathology , Status Epilepticus/etiology , Seizures, Febrile/pathology , Seizures, Febrile/diagnostic imaging , Infant , Child, Preschool , Child , Follow-Up Studies , Atrophy/pathology , Hippocampal Sclerosis
2.
Brain ; 146(8): 3444-3454, 2023 08 01.
Article in English | MEDLINE | ID: mdl-37143309

ABSTRACT

Brain oedema is a life-threatening complication of various neurological conditions. Understanding molecular mechanisms of brain volume regulation is critical for therapy development. Unique insight comes from monogenic diseases characterized by chronic brain oedema, of which megalencephalic leukoencephalopathy with subcortical cysts (MLC) is the prototype. Variants in MLC1 or GLIALCAM, encoding proteins involved in astrocyte volume regulation, are the main causes of MLC. In some patients, the genetic cause remains unknown. We performed genetic studies to identify novel gene variants in MLC patients, diagnosed by clinical and MRI features, without MLC1 or GLIALCAM variants. We determined subcellular localization of the related novel proteins in cells and in human brain tissue. We investigated functional consequences of the newly identified variants on volume regulation pathways using cell volume measurements, biochemical analysis and electrophysiology. We identified a novel homozygous variant in AQP4, encoding the water channel aquaporin-4, in two siblings, and two de novo heterozygous variants in GPRC5B, encoding the orphan G protein-coupled receptor GPRC5B, in three unrelated patients. The AQP4 variant disrupts membrane localization and thereby channel function. GPRC5B, like MLC1, GlialCAM and aquaporin-4, is expressed in astrocyte endfeet in human brain. Cell volume regulation is disrupted in GPRC5B patient-derived lymphoblasts. GPRC5B functionally interacts with ion channels involved in astrocyte volume regulation. In conclusion, we identify aquaporin-4 and GPRC5B as old and new players in genetic brain oedema. Our findings shed light on the protein complex involved in astrocyte volume regulation and identify GPRC5B as novel potentially druggable target for treating brain oedema.


Subject(s)
Brain Edema , Hereditary Central Nervous System Demyelinating Diseases , Humans , Membrane Proteins/genetics , Brain Edema/genetics , Brain Edema/metabolism , Mutation/genetics , Hereditary Central Nervous System Demyelinating Diseases/genetics , Brain/metabolism , Astrocytes/metabolism , Aquaporin 4/genetics , Aquaporin 4/metabolism , Receptors, G-Protein-Coupled/genetics , Receptors, G-Protein-Coupled/metabolism
3.
Dev Med Child Neurol ; 2024 May 08.
Article in English | MEDLINE | ID: mdl-38718250

ABSTRACT

Accurate determination of death is a necessary responsibility of the medical profession. Brain death, or death by neurological criteria (DNC), can be legally declared after the determination of permanent loss of clinical brain function, including the capacity for consciousness, brainstem reflexes, and the ability to breathe spontaneously. Despite longstanding debates over the exact definition of brain death or DNC and how it is determined, most middle- and high-income countries have compatible medical protocols and legal policies for brain death or DNC. This review summarizes the 2023 updated guidelines for brain death or DNC determination, which integrate adult and pediatric diagnostic criteria. We discuss the clinical challenges related to brain death or DNC determination in infants and young children. We emphasize that physicians must follow the standardized and meticulous evaluation processes outlined in these guidelines to reduce diagnostic error and ensure no false positive determinations. An essential component of the brain death or DNC evaluation is appropriate and transparent communication with families. Ongoing efforts to promote consistency and legal uniformity in the declaration of death are needed.

4.
Dev Med Child Neurol ; 65(7): 900-907, 2023 07.
Article in English | MEDLINE | ID: mdl-36732680

ABSTRACT

The aim of this review was to discuss bioethics in prenatal diagnosis and health care after recent legislative and judicial changes affecting reproductive rights, such as the repeal of 'Roe v. Wade' in the United States. We recognize that abortion involves particular moralities that are not universal or shared by all cultures, groups, and individuals. We reviewed the historical aspects of embryology and personhood, fetal morbidity and mortality, and parental options for prenatal diagnostic testing. We examined relevant ethical issues including informed consent, the emergence of fetal pain, reproductive autonomy, the fiduciary responsibilities of pregnant mothers, and the obligations of physicians caring for the maternal-fetal dyad. The code of medical ethics includes respect for decisional privacy and the protection of information shared in confidence. When a fetal anomaly is diagnosed, pregnant mothers must be informed about the risks, burdens, and alternatives in either continuing or terminating the pregnancy. Parental choice should include the right to refuse testing, the informed choice not to know about certain genetic test results, and the right to make informed decisions about the best interests of the future child. In the diagnosis and care of fetal anomalies, moral dilemmas arise. Before fetal viability, the mother's autonomy, sense of beneficence, and personal values should be trusted and respected. Perinatal palliative care should be available to pregnant mothers whose anomalous fetus is carried to birth.


Subject(s)
Abortion, Induced , Pregnant Women , Pregnancy , Female , Child , Humans , United States , Prenatal Diagnosis , Ethics, Medical , Personhood , Fetus
5.
Hum Mutat ; 40(7): 908-925, 2019 07.
Article in English | MEDLINE | ID: mdl-30817854

ABSTRACT

Pathogenic de novo variants in the X-linked gene SLC35A2 encoding the major Golgi-localized UDP-galactose transporter required for proper protein and lipid glycosylation cause a rare type of congenital disorder of glycosylation known as SLC35A2-congenital disorders of glycosylation (CDG; formerly CDG-IIm). To date, 29 unique de novo variants from 32 unrelated individuals have been described in the literature. The majority of affected individuals are primarily characterized by varying degrees of neurological impairments with or without skeletal abnormalities. Surprisingly, most affected individuals do not show abnormalities in serum transferrin N-glycosylation, a common biomarker for most types of CDG. Here we present data characterizing 30 individuals and add 26 new variants, the single largest study involving SLC35A2-CDG. The great majority of these individuals had normal transferrin glycosylation. In addition, expanding the molecular and clinical spectrum of this rare disorder, we developed a robust and reliable biochemical assay to assess SLC35A2-dependent UDP-galactose transport activity in primary fibroblasts. Finally, we show that transport activity is directly correlated to the ratio of wild-type to mutant alleles in fibroblasts from affected individuals.


Subject(s)
Congenital Disorders of Glycosylation/genetics , Monosaccharide Transport Proteins/genetics , Monosaccharide Transport Proteins/metabolism , Uridine Diphosphate Galactose/metabolism , Animals , Biopsy , CHO Cells , Cells, Cultured , Congenital Disorders of Glycosylation/metabolism , Congenital Disorders of Glycosylation/pathology , Cricetulus , Female , Humans , Male , Mutation
6.
Epilepsia ; 58(6): 1102-1111, 2017 06.
Article in English | MEDLINE | ID: mdl-28448686

ABSTRACT

OBJECTIVE: Our aim was to explore the association between plasma cytokines and febrile status epilepticus (FSE) in children, as well as their potential as biomarkers of acute hippocampal injury. METHODS: Analysis was performed on residual samples of children with FSE (nĀ =Ā 33) asĀ part of the Consequences of Prolonged Febrile Seizures in Childhood study (FEBSTAT) and compared to children with fever (nĀ =Ā 17). Magnetic resonance imaging (MRI) was obtained as part of FEBSTAT within 72Ā h of FSE. Cytokine levels and ratios of antiinflammatory versus proinflammatory cytokines in children with and without hippocampal T2 hyperintensity were assessed as biomarkers of acute hippocampal injury after FSE. RESULTS: Levels of interleukin (IL)-8 and epidermal growth factor (EGF) were significantly elevated after FSE in comparison to controls. IL-1Ɵ levels trended higher and IL-1RA trended lower following FSE, but did not reach statistical significance. Children with FSE were found to have significantly lower ratios of IL-1RA/IL-1Ɵ and IL-1RA/IL-8. Specific levels of any one individual cytokine were not associated with FSE. However, lower ratios of IL-1RA/IL-1Ɵ, IL-1RA/1L-6, and IL-1RA/ IL-8 were all associated with FSE. IL-6 and IL-8 levels were significantly higher and ratios of IL-1RA/IL-6 and IL-1RA/IL-8 were significantly lower in children with T2 hippocampal hyperintensity on MRI after FSE in comparison to those without hippocampal signal abnormalities. Neither individual cytokine levels nor ratios of IL-1RA/IL-1Ɵ or IL-1RA/IL-8 were predictive of MRI changes. However, a lower ratio of IL-1RA/IL-6 was strongly predictive (odds ratio [OR] 21.5, 95% confidence interval [CI] 1.17-393) of hippocampal T2 hyperintensity after FSE. SIGNIFICANCE: Our data support involvement of the IL-1 cytokine system, IL-6, and IL-8 in FSE in children. The identification of the IL-1RA/IL-6 ratio as a potential biomarker of acute hippocampal injury following FSE is the most significant finding. If replicated in another study, the IL-1RA/IL-6 ratio could represent a serologic biomarker that offers rapid identification of patients at risk for ultimately developing mesial temporal lobe epilepsy (MTLE).


Subject(s)
Biomarkers/blood , Brain Damage, Chronic/blood , Cytokines/blood , Hippocampus/diagnostic imaging , Hippocampus/physiopathology , Seizures, Febrile/blood , Status Epilepticus/blood , Brain Damage, Chronic/diagnostic imaging , Child , Child, Preschool , Epilepsy, Temporal Lobe/blood , Female , Humans , Infant , Infant, Newborn , Interleukin 1 Receptor Antagonist Protein/blood , Interleukin-1beta/blood , Interleukin-6/blood , Interleukin-8/blood , Male , Risk Factors , Seizures, Febrile/diagnostic imaging , Status Epilepticus/diagnostic imaging
7.
Epilepsia ; 57(7): 1042-7, 2016 07.
Article in English | MEDLINE | ID: mdl-27265870

ABSTRACT

OBJECTIVES: To identify risk and risk factors for developing a subsequent febrile seizure (FS) in children with a first febrile status epilepticus (FSE) compared to a first simple febrile seizure (SFS). To identify home use of rescue medications for subsequent FS. METHODS: Cases included a first FS that was FSE drawn from FEBSTAT and Columbia cohorts. Controls were a first SFS. Cases and controls were classified according to established FEBSTAT protocols. Cumulative risk for subsequent FS over a 5-year period was compared in FSE versus SFS, and Cox proportional hazards regression was conducted. Separate analysis examined subsequent FS within FSE. The use of rescue medications at home was assessed for subsequent FS. RESULTS: Risk for a subsequent FSE was significantly increased in FSE versus SFS. Any magnetic resonance imaging (MRI) abnormality increased the risk 3.4-fold (p < 0.05), adjusting for age at first FS and FSE and in analyses restricted to children whose first FS was FSE (any MRI abnormality hazard ratio [HR] 2.9, p < 0.05). The risk for a second FS of any type or of subsequent FS lasting >10 min over the 5-year follow-up did not differ in FSE versus SFS. Rectal diazepam was administered at home to 5 (23.8%) of 21 children with subsequent FS lasting ≥10 min. SIGNIFICANCE: Compared to controls, FSE was associated with an increased risk for subsequent FSE, suggesting the propensity of children with an initial prolonged seizure to experience a prolonged recurrence. Any baseline MRI abnormality increased the recurrence risk when FSE was compared to SFS and when FSE was studied alone. A minority of children with a subsequent FS lasting 10 min or longer were treated with rectal diazepam at home, despite receiving prescriptions after the first FSE. This indicates the need to further improve the education of clinicians and parents in order to prevent subsequent FSE.


Subject(s)
Seizures, Febrile/epidemiology , Seizures, Febrile/etiology , Status Epilepticus/complications , Child, Preschool , Cohort Studies , Electroencephalography , Female , Humans , Infant , Magnetic Resonance Imaging , Male , Proportional Hazards Models , Regression Analysis , Risk Factors , Seizures, Febrile/diagnosis , Status Epilepticus/diagnosis , Status Epilepticus/epidemiology
8.
Epilepsy Behav ; 64(Pt A): 283-288, 2016 11.
Article in English | MEDLINE | ID: mdl-27794237

ABSTRACT

OBJECTIVE: The objective of this study was to determine early developmental and cognitive outcomes of children with febrile status epilepticus (FSE) one month and one year after FSE. METHODS: One hundred ninety four children with FSE were evaluated on measures of cognition, receptive language, and memory as part of the FEBSTAT study and compared with 100 controls with simple febrile seizures (FSs). RESULTS: Children with FSE did not differ dramatically on tasks compared with FS controls at one month after FSE but demonstrated slightly weaker motor development (p=0.035) and receptive language (p=0.034) at one year after FSE. Performances were generally within the low average to average range. Within the FSE cohort, non-White children performed weaker on many of the tasks compared with Caucasian children. At the one-year visit, acute hippocampal T2 findings on MRI were associated with weaker receptive language skills (p=0.0009), and human herpes virus 6 or 7 (HHV6/7) viremia was associated with better memory performances (p=0.047). CONCLUSION: Febrile status epilepticus does not appear to be associated with significant cognitive impairment on early developmental measures, although there is a trend for possible receptive language and motor delay one year after FSE. Further follow-up, which is in progress, is necessary to track long-term cognitive functioning.


Subject(s)
Cognition/physiology , Language , Memory/physiology , Seizures, Febrile/psychology , Status Epilepticus/psychology , Child, Preschool , Female , Hippocampus/diagnostic imaging , Humans , Infant , Magnetic Resonance Imaging , Male , Neuroimaging , Neuropsychological Tests , Seizures, Febrile/complications , Seizures, Febrile/diagnostic imaging , Status Epilepticus/complications , Status Epilepticus/diagnostic imaging
9.
Ann Neurol ; 75(2): 178-85, 2014 Feb.
Article in English | MEDLINE | ID: mdl-24318290

ABSTRACT

OBJECTIVE: Whether febrile status epilepticus (FSE) produces hippocampal sclerosis (HS) and temporal lobe epilepsy (TLE) has long been debated. Our objective is to determine whether FSE produces acute hippocampal injury that evolves to HS. METHODS: FEBSTAT and 2 affiliated studies prospectively recruited 226 children aged 1 month to 6 years with FSE and controls with simple febrile seizures. All had acute magnetic resonance imaging (MRI), and follow-up MRI was obtained approximately 1 year later in the majority. Visual interpretation by 2 neuroradiologists informed only of subject age was augmented by hippocampal volumetrics, analysis of the intrahippocampal distribution of T2 signal, and apparent diffusion coefficients. RESULTS: Hippocampal T2 hyperintensity, maximum in Sommer's sector, occurred acutely after FSE in 22 of 226 children in association with increased volume. Follow-up MRI obtained on 14 of the 22 with acute T2 hyperintensity showed HS in 10 and reduced hippocampal volume in 12. In contrast, follow-up of 116 children without acute hyperintensity showed abnormal T2 signal in only 1 (following another episode of FSE). Furthermore, compared to controls with simple febrile seizures, FSE subjects with normal acute MRI had abnormally low right to left hippocampal volume ratios, smaller hippocampi initially, and reduced hippocampal growth. INTERPRETATION: Hippocampal T2 hyperintensity after FSE represents acute injury often evolving to a radiological appearance of HS after 1 year. Furthermore, impaired growth of normal-appearing hippocampi after FSE suggests subtle injury even in the absence of T2 hyperintensity. Longer follow-up is needed to determine the relationship of these findings to TLE.


Subject(s)
Hippocampus/pathology , Status Epilepticus/complications , Status Epilepticus/pathology , Child , Child, Preschool , Diffusion Magnetic Resonance Imaging , Female , Follow-Up Studies , Humans , Infant , Magnetic Resonance Imaging , Male , Prospective Studies , Risk Factors , Sclerosis/etiology
10.
Mol Genet Metab Rep ; 38: 101051, 2024 Mar.
Article in English | MEDLINE | ID: mdl-38469093

ABSTRACT

Riboflavin transporter deficiency (RTD) is a neurodegenerative disorder that presents from infancy to adulthood with a progressive axonal neuropathy characterized by a variety of neurologic symptoms including hearing loss, weakness, bulbar palsy, and respiratory insufficiency. Pathogenic variants in SLC52A2 and SLC52A3 are implicated in the pathogenesis of RTD type 2 and 3, respectively. Early identification of this disorder is critical, as it is treatable with riboflavin supplementation. We describe a 16-year-old female with a phenotype consistent with RTD3 found to have a novel heterozygous SLC52A3 variant. Though RTD is typically considered an autosomal recessive condition, her heterozygous variant was thought to be disease causing after further genetic analysis and given her improvement in response to riboflavin supplementation. This case highlights the importance of reinterpretation of genetic testing, particularly when there is a high clinical suspicion for disease.

11.
J Neurovirol ; 19(5): 452-60, 2013 Oct.
Article in English | MEDLINE | ID: mdl-23979706

ABSTRACT

Matrix metalloproteinases (MMPs) have been implicated in human immunodeficiency virus (HIV)-associated neurological injury; however, this relationship has not been studied early in infection. Plasma levels of MMP-1, MMP-2, MMP-7, MMP-9, and MMP-10 measured using Luminex technology (Austin, TX, USA) were compared in 52 HIV and 21 seronegative participants of the Chicago Early HIV Infection study. MMP levels were also examined in HIV subgroups defined by antibody reactivity, viremia, and antiretroviral status, as well as in available cerebrospinal fluid (CSF) samples (n = 9). MMPs were evaluated for patterns of relationship to cognitive function and to quantitative magnetic resonance measurements of the brain derived in vivo. Plasma MMP-2 levels were significantly reduced in early HIV infection and correlated with altered white matter integrity and atrophic brain changes. MMP-9 levels were higher in the treated subgroup than in the naĆÆve HIV subgroup. Only MMP-2 and MMP-9 were detected in the CSF; CSF MMP-2 correlated with white matter integrity and with volumetric changes in basal ganglia. Relationships with cognitive function were also identified. MMP-2 levels in plasma and in CSF correspond to early changes in brain structure and function. These findings establish a link between MMPs and neurological status previously unidentified in early HIV infection.


Subject(s)
Basal Ganglia/enzymology , Cognition Disorders/enzymology , HIV Infections/enzymology , HIV , Adult , Basal Ganglia/pathology , Biomarkers/blood , Biomarkers/cerebrospinal fluid , Cognition Disorders/diagnosis , Cognition Disorders/pathology , Cognition Disorders/psychology , Early Diagnosis , Female , HIV Infections/diagnosis , HIV Infections/pathology , HIV Infections/psychology , Humans , Magnetic Resonance Imaging , Male , Matrix Metalloproteinase 1/blood , Matrix Metalloproteinase 1/cerebrospinal fluid , Matrix Metalloproteinase 10/blood , Matrix Metalloproteinase 10/cerebrospinal fluid , Matrix Metalloproteinase 2/blood , Matrix Metalloproteinase 2/cerebrospinal fluid , Matrix Metalloproteinase 7/blood , Matrix Metalloproteinase 7/cerebrospinal fluid , Matrix Metalloproteinase 9/blood , Matrix Metalloproteinase 9/cerebrospinal fluid , Neuropsychological Tests
12.
J Pediatr ; 161(6): 1169-71, 2012 Dec.
Article in English | MEDLINE | ID: mdl-22985722

ABSTRACT

This prospective multicenter study of 200 patients with fever-associated status epilepticus (FSE), of whom 136 underwent a nontraumatic lumbar puncture, confirms that FSE rarely causes cerebrospinal fluid (CSF) pleocytosis. CSF glucose and protein levels were unremarkable. Temperature, age, seizure focality, and seizure duration did not affect results. CSF pleocytosis should not be attributed to FSE.


Subject(s)
Seizures, Febrile/cerebrospinal fluid , Status Epilepticus/cerebrospinal fluid , Biomarkers/cerebrospinal fluid , Cerebrospinal Fluid Proteins/metabolism , Child , Glucose/cerebrospinal fluid , Humans , Leukocytes/metabolism , Leukocytosis/cerebrospinal fluid , Leukocytosis/etiology , Prospective Studies , Seizures, Febrile/physiopathology , Spinal Puncture , Status Epilepticus/etiology
13.
Epilepsia ; 53(9): 1481-8, 2012 Sep.
Article in English | MEDLINE | ID: mdl-22954016

ABSTRACT

PURPOSE: In a prospective study, Consequences of Prolonged Febrile Seizures in Childhood (FEBSTAT), we determined the frequency of human herpesvirus (HHV)-6 and HHV-7 infection as a cause of febrile status epilepticus (FSE). METHODS: Children ages 1 month to 5 years presenting with FSE were enrolled within 72 h and received a comprehensive assessment including specimens for HHV-6 and HHV-7. The presence of HHV-6A, HHV-6B, or HHV-7 DNA and RNA (amplified across a spliced junction) determined using quantitative polymerase chain reaction (qPCR) at baseline indicated viremia. Antibody titers to HHV-6 and HHV-7 were used in conjunction with the PCR results to distinguish primary infection from reactivated or prior infection. KEY FINDINGS: Of 199 children evaluated, HHV-6 or HHV-7 status could be determined in 169 (84.9%). HHV-6B viremia at baseline was found in 54 children (32.0%), including 38 with primary infection and 16 with reactivated infection. No HHV-6A infections were identified. HHV-7 viremia at baseline was observed in 12 children (7.1%), including eight with primary infection and four with reactivated infection. Two subjects had HHV-6/HHV-7 primary coinfection at baseline. There were no differences in age, characteristics of illness or fever, seizure phenomenology or the proportion of acute EEG or imaging abnormalities in children presenting with FSE with or without HHV infection. SIGNIFICANCE: HHV-6B infection is commonly associated with FSE. HHV-7 infection is less frequently associated with FSE. Together, they account for one third of FSE, a condition associated with an increased risk of both hippocampal injury and subsequent temporal lobe epilepsy.


Subject(s)
Herpesvirus 6, Human , Herpesvirus 7, Human , Roseolovirus Infections/epidemiology , Seizures, Febrile/epidemiology , Status Epilepticus/epidemiology , Child, Preschool , Female , Herpesvirus 6, Human/isolation & purification , Herpesvirus 7, Human/isolation & purification , Humans , Infant , Infant, Newborn , Male , Prospective Studies , Roseolovirus Infections/diagnosis , Seizures, Febrile/diagnosis , Seizures, Febrile/virology , Status Epilepticus/diagnosis , Status Epilepticus/virology
14.
Epilepsia ; 53(9): 1471-80, 2012 Sep.
Article in English | MEDLINE | ID: mdl-22742587

ABSTRACT

PURPOSE: Febrile status epilepticus (FSE) has been associated with hippocampal injury and subsequent hippocampal sclerosis (HS) and temporal lobe epilepsy. The FEBSTAT study was designed to prospectively examine the association between prolonged febrile seizures and development of HS and associated temporal lobe epilepsy, one of the most controversial issues in epilepsy. We report on the baseline phenomenology of the final cohorts as well as detailed aims and methodology. METHODS: The "Consequences of Prolonged Febrile Seizures in Childhood" (FEBSTAT) study is a prospective, multicenter study. Enrolled are children, aged 1 month to 6 years of age, presenting with a febrile seizure lasting 30 min or longer based on ambulance, emergency department, and hospital records, and parental interview. At baseline, procedures included a magnetic resonance imaging (MRI) study and electroencephalography (EEG) recording done within 72 h of FSE, and a detailed history and neurologic examination. Baseline development and behavior are assessed at 1 month. The baseline assessment is repeated, with age-appropriate developmental testing at 1 and 5 years after enrollment as well as at the development of epilepsy and 1 year after that. Telephone calls every 3 months document additional seizures. Two other groups of children are included: a "control" group consisting of children with a first febrile seizure ascertained at Columbia University and with almost identical baseline and 1-year follow-up examinations and a pilot cohort of FSE from Duke University. KEY FINDINGS: The FEBSTAT cohort consists of 199 children with a median age at baseline of 16.0 months (interquartile range [IQR] 12.0-24.0) and a median duration of FSE of 70.0 min (IQR 47.0-110.0). Seizures were continuous in 57.3% and behaviorally intermittent (without recovery in between) in 31.2%; most were partial (2.0%) or secondary generalized (65.8%), and almost all (98.0%) culminated in a generalized tonic-clonic seizure. Of the 199 children, 86.4% had normal development and 20% had prior febrile seizures. In one third of cases, FSE was unrecognized in the emergency department. The Duke existing cohort consists of 23 children with a median age of FSE onset of 18.0 months (IQR 14.0-28.0) and median duration of FSE of 90.0 min (IQR 50.0-170.0). The Columbia control cohort consists of 159 children with a first febrile seizure who received almost the same workup as the FEBSTAT cohort at baseline and at 1 year. They were followed by telephone every 4 months for a median of 42 months. Among the control cohort, 64.2% had a first simple FS, 26.4% had a first complex FS that was not FSE, and 9.4% had FSE. Among the 15 with FSE, the median age at onset was 14.0 months (IQR 12.0-20.0) and the median duration of FSE was 43.0 min (IQR 35.0-75.0). SIGNIFICANCE: The FEBSTAT study presents an opportunity to prospectively study the relationship between FSE and acute hippocampal damage, the development of mesial temporal sclerosis, epilepsy (particularly temporal lobe epilepsy), and impaired hippocampal function in a large cohort. It is hoped that this study may illuminate a major mystery in clinical epilepsy today, and permit the development of interventions designed to prevent the sequelae of FSE.


Subject(s)
Research Design , Seizures, Febrile/diagnosis , Seizures, Febrile/epidemiology , Child , Child, Preschool , Cohort Studies , Female , Humans , Infant , Infant, Newborn , Male , Pilot Projects , Prospective Studies , Seizures, Febrile/therapy
16.
J Neurovirol ; 17(2): 153-8, 2011 Apr.
Article in English | MEDLINE | ID: mdl-21302026

ABSTRACT

Circulating levels of matrix metalloproteinases (MMP-1 and 7) have been found to correlate with the severity of brain injury in HIV-infected subjects. This study used high-resolution neuroanatomic imaging and automated segmentation algorithms to clarify this relationship. Both metalloproteinases were significantly correlated with increased cerebrospinal fluid volume fraction. Comprehensive brain volumetric analysis revealed a more marked relationship with atrophy for MMP-7, which was significantly correlated with neural injury in multiple brain regions and nearly all ventricular measurements. MMP-7 was also correlated with measures of virologic and cognitive status.


Subject(s)
AIDS Dementia Complex/metabolism , Brain/metabolism , Cone-Beam Computed Tomography/methods , Magnetic Resonance Imaging/methods , Matrix Metalloproteinase 7 , AIDS Dementia Complex/pathology , AIDS Dementia Complex/virology , Algorithms , Atrophy , Automation, Laboratory , Brain/pathology , Brain/virology , CD4-Positive T-Lymphocytes/pathology , Cell Count , Cognition , Female , HIV/physiology , HIV Infections/metabolism , HIV Infections/pathology , HIV Infections/virology , Humans , Male , Matrix Metalloproteinase 2/biosynthesis , Matrix Metalloproteinase 2/cerebrospinal fluid , Matrix Metalloproteinase 7/biosynthesis , Matrix Metalloproteinase 7/cerebrospinal fluid , Middle Aged , Viral Load
17.
Neurology ; 97(14): 685-692, 2021 10 05.
Article in English | MEDLINE | ID: mdl-35294396

ABSTRACT

High drug prices have created substantial challenges for patients, physicians, health systems, and payers. High drug prices can affect patient care in many ways, including limiting access to treatment, increasing the burden of administrative tasks, and contributing to physician burnout. Exorbitant drug pricing poses direct challenges for distributive justice, which is concerned with fairly distributing benefits and burdens across society. In this position statement, we discuss ethical concerns raised by high drug costs, primarily focusing on concerns around distributive justice. We consider forms of rationing, approaches to allocation, potential complexities in real-life application, and structural forces contributing to high drug costs. Finally, we consider potential policy solutions and ramifications for individual clinicians.


Subject(s)
Drug Costs , Humans
18.
Neurology ; 94(15): 661-669, 2020 04 14.
Article in English | MEDLINE | ID: mdl-32179700

ABSTRACT

This update to the American Academy of Neurology's 1998 position statement endeavors to provide guidance for the consistent ethical conduct and review of neurologic research involving human participants. It does so by outlining a widely used ethical framework of 7 principles derived from the foundational documents of modern bioethics, including the Nuremberg Code, the World Medical Association's Declaration of Helsinki, the Belmont Report, and the US Department of Health and Human Service's Common Rule. The position statement then applies this principle-based framework to analyze and produce recommendations for the management of common and important ethical issues encountered in neurologic clinical research. These include institutional review board oversight, equitable research participant inclusion, cognitive impairment in research participants, international studies, the replication crisis, and genetic testing and modification.


Subject(s)
Bioethics , Biomedical Research/ethics , Informed Consent/ethics , Neurology/ethics , Humans , Societies, Medical/ethics , United States
19.
Neurology ; 95(4): 167-172, 2020 07 28.
Article in English | MEDLINE | ID: mdl-32414880

ABSTRACT

Patients, clinicians, and hospitals have undergone monumental changes during the coronavirus disease 2019 (COVID-19) pandemic. This crisis has forced us to consider the obligations that we neurologists have to our individual patients as well as the greater community. By returning to our fundamental understanding of these duties, we can ensure that we are providing the most ethically appropriate contingency and crisis care possible. We recommend specific adaptations to both the inpatient and outpatient settings, as well as changes to medical and trainee education. Furthermore, we explore the daunting but potentially necessary implementation of scare resource allocation protocols. As the pandemic evolves, we will need to adapt continuously to these rapidly changing circumstances and consider both national and regional standards and variation.


Subject(s)
Coronavirus Infections/therapy , Neurologists/ethics , Neurology/ethics , Pandemics/ethics , Pneumonia, Viral/therapy , COVID-19 , Coronavirus Infections/complications , Health Resources , Humans , Nervous System Diseases/complications , Neurology/education , Pneumonia, Viral/complications , Referral and Consultation , Societies, Medical , Telemedicine
20.
J Neurovirol ; 15(3): 275-81, 2009 May.
Article in English | MEDLINE | ID: mdl-19444696

ABSTRACT

Circulating levels of specific matrix metalloproteinases (MMPs; 1 and 7) were evaluated as correlates of brain injury in eight individuals in advanced human immunodeficiency virus (HIV) infection. Neurological status was quantified in vivo with automated segmentation algorithms and with diffusion tensor imaging. Both metalloproteinases correlated with microstructural brain alterations and the degree of atrophy. MMPs may influence neurological outcome through involvement in neuroimmune response, blood-brain barrier permeability, leukocyte migration, and MMP-mediated neurotoxicity.


Subject(s)
AIDS Dementia Complex/pathology , Brain/pathology , Matrix Metalloproteinase 1/blood , Matrix Metalloproteinase 7/blood , AIDS Dementia Complex/blood , Adult , Atrophy/pathology , Atrophy/virology , Biomarkers/blood , Brain/virology , Diffusion Tensor Imaging , Female , Humans , Magnetic Resonance Imaging , Male , Middle Aged
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