Genome-Wide Association Analysis for Chronic Superficial Keratitis in the Australian Racing Greyhound.

Chronic superficial keratitis (CSK) is a progressive inflammatory condition of the eye (cornea) that can cause discomfort and blindness. Differential disease risk across dog breeds strongly suggests that CSK has a genetic basis. In addition to genetic risk, the occurrence of CSK is exacerbated by exposure to ultraviolet light. Genome-wide association analysis considered 109 greyhounds, 70 with CSK and the remainder with normal phenotype at an age over four years. Three co-located variants on CFA18 near the 5' region of the Epidermal Growth Factor Receptor (EGFR) gene were associated with genome-wide significance after multiple-test correction (BICF2P579527, CFA18: 6,068,508, praw = 1.77 × 10-7, pgenome = 0.017; BICF2P1310662, CFA18: 6,077,388, praw = 4.09 × 10-7, pgenome = 0.040; BICF2P160719, CFA18: 6,087,347, praw = 4.09 × 10-7, pgenome = 0.040) (canFam4)). Of the top 10 associated markers, eight were co-located with the significantly associated markers on CFA18. The associated haplotype on CFA18 is protective for the CSK condition. EGFR is known to play a role in corneal healing, where it initiates differentiation and proliferation of epithelial cells that in turn signal the involvement of stromal keratocytes to commence apoptosis. Further validation of the putative functional variants is required prior to their use in genetic testing for breeding programs.

Outcome of Allogeneic Adult Stem Cell Therapy in Dogs Suffering from Osteoarthritis and Other Joint Defects.

Osteoarthritis is a common condition that causes joint pain and stiffness that affects both humans and dogs. In Australia, allogeneic canine adipose-derived mesenchymal stem cells for therapy have been commercially available since 2010. In this report, we describe the outcome of the treatment of two hundred and three dogs diagnosed with degenerative arthritis with severe chronic pain in joints causing lameness at walk, reduced mobility, and functional disability. Posttreatment assessment data after 10 weeks revealed significant improvement (p < 0.007) of the symptoms: pain reduction, improvement of mobility, and increased daily activity as measured as quality of life score. Ninety percent of young dogs (<9 years) showed excellent improvement in pain and mobility and were able to run and resume normal activity. Sixty percent of older dogs showed good improvement. However, 12% of dogs did not exhibit any change in symptoms; one dog showed worsening of the symptoms. This report provides the support for the safety and efficacies of allogeneic adipose-derived mesenchymal stem cells in a regenerative therapeutic veterinary model.

Public perceptions of the threat of terrorist attack in Australia and anticipated compliance behaviours.

OBJECTIVE: To determine the perceived threat of terrorist attack in Australia and preparedness to comply with public safety directives. METHODS: A representative sample of 2,081 adults completed terrorism perception questions as part of the New South Wales Population Health Survey. RESULTS: Overall, 30.3% thought a terrorist attack in Australia was highly likely, 42.5% were concerned that self or family would be directly affected and 26.4% had changed the way they lived due to potential terrorist attacks. Respondents who spoke a language other than English at home were 2.47 times (Odds Ratios (OR=2.47, 95% CI:1.58-3.64, p<0.001) more likely to be concerned self or family would be affected and 2.88 times (OR=2.88, 95% CI:1.95-4.25, p<0.001) more likely to have changed the way they lived due to the possibility of terrorism. Those with high psychological distress perceived higher terrorism likelihood and greater concern that self or family would be directly affected (OR=1.84, 95% CI:1.05-3.22, p=0.034). Evacuation willingness was high overall but those with poor self-rated health were significantly less willing to leave their homes during a terrorism emergency. CONCLUSION: Despite not having experienced recent terrorism within Australia, perceived likelihood of an attack was higher than in comparable western countries. Marginalisation of migrant groups associated with perceived terrorism threat may be evident in the current findings. IMPLICATIONS: This baseline data will be useful to monitor changes in population perceptions over time and determine the impact of education and other preparedness initiatives.