Microbiological evaluation in invisible aligner chemical cleaning methods against Candida albicans and Streptococcus mutans.

INTRODUCTION: This study aimed to assess the efficacy of chemical agents in removing Candida albicans and Streptococcus mutans biofilm from invisible aligners. METHODS: The samples were made of EX30 Invisalign trays, biofilm was cultured by standardized suspensions of C. albicans ATCC strain and S. mutans clinical strain on the sample. The treatments used were 0.5% sodium hypochlorite (NaClO) (20 minutes), 1% NaClO (10 minutes), chlorhexidine (5 minutes), peroxide (15 minutes), and orthophosphoric acid (15 seconds). The control group received phosphate-buffered saline for 10 minutes. The colony-forming units per milliliter of each microorganism were determined by serial dilutions seeded in plates with selective culture mediums for each one. Data were analyzed by the Kruskal-Wallis and Conover-Iman tests at an α of 0.05. RESULTS: For the C. albicans biofilm group, the control group had 9.7 Log10 of microorganism growth, and all treatment groups had statistically significant biofilm reduction, in which chlorhexidine presented the highest inhibition of 3 Log10, followed by alkaline peroxide and orthophosphoric acid both with 2.6 Log10, 1% NaClO (2.5 Log10), and 0.5% NaClO (2 Log10). As for S. mutans, the control group had 8.9 Log10 of growth, and a total microorganism inhibition was reached by chlorhexidine, 1% NaClO, and orthophosphoric acid, whereas alkaline peroxide inhibited growth to 7.9 Log10 and 0.5% NaClO 5.1 Log10. CONCLUSIONS: Within the limitations, chlorhexidine and orthophosphoric acid had greater efficacy in both biofilms. In addition, 1% NaClO and alkaline peroxide also had significant effects; therefore, their incorporation aligners disinfection protocols are valid.

Prevalence of condylar morphological changes in individuals with class II malocclusion.

This observational, cross-sectional study with retrospective collection was aimed to evaluate the prevalence of morphological changes in mandibular condyles in individuals with class II malocclusion, classified according to different vertical growth patterns (brachyfacial, mesofacial, and dolichofacial), through cone beam computed tomography (CBCT). Seventy CBCT images (140 mandibular condyles) were selected from individuals without orthodontic treatment, of both sexes, aged between 25 and 50 years. No statistically significant differences were found between facial patterns; however, there was a higher relative prevalence of bone changes in dolichofacial individuals with flattening (62%), sclerosis (44%), and subchondral bone cyst (20%). Erosion and osteophytes prevailed in mesofacial (39%), and brachyfacial individuals (32%), respectively. Thus, there was no statistically significant difference in the prevalence of degenerative changes between the vertical skeletal patterns. Flattening was the most prevalent change, whereas subchondral bone cyst was the least prevalent among the three groups studied. The observational design of this study makes it possible to analyze image banks to verify the correlation of morphological changes in the temporomandibular joint in different facial patterns in patients with class II malocclusion. A limitation of the study is that clinical characteristics were not evaluated.

Prevalence of condylar morphological changes in individuals with class II malocclusion

Abstract This observational, cross-sectional study with retrospective collection was aimed to evaluate the prevalence of morphological changes in mandibular condyles in individuals with class II malocclusion, classified according to different vertical growth patterns (brachyfacial, mesofacial, and dolichofacial), through cone beam computed tomography (CBCT). Seventy CBCT images (140 mandibular condyles) were selected from individuals without orthodontic treatment, of both sexes, aged between 25 and 50 years. No statistically significant differences were found between facial patterns; however, there was a higher relative prevalence of bone changes in dolichofacial individuals with flattening (62%), sclerosis (44%), and subchondral bone cyst (20%). Erosion and osteophytes prevailed in mesofacial (39%), and brachyfacial individuals (32%), respectively. Thus, there was no statistically significant difference in the prevalence of degenerative changes between the vertical skeletal patterns. Flattening was the most prevalent change, whereas subchondral bone cyst was the least prevalent among the three groups studied. The observational design of this study makes it possible to analyze image banks to verify the correlation of morphological changes in the temporomandibular joint in different facial patterns in patients with class II malocclusion. A limitation of the study is that clinical characteristics were not evaluated.

Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices / Análise da formação de biofilme por Candida albicans em diferentes tipos de aparelhos e dispositivos ortodônticos fixos

Objective: in this study, biofilm formation by Candida albicans in fixed orthodontic appliances was evaluated. Material and Methods: a total of 300 conventional metal brackets (MC), ceramic (CB), self-ligation (SLB), nickel-titanium (NiTi), and nickel-chromium (NiCr) wires, and ligatures types were organized into thirty groups (n=10). To induce biofilm formation, brackets, wires, and ligatures were joined, sterilized, placed in 24-well plates, contaminated with standardized suspensions of C. albicans (107 cells/mL), and incubated at 37 °C for 48 h with shaking. The biofilms formed were detached using an ultrasonic homogenizer, and suspensions were serially diluted and plated on Sabouraud dextrose agar to determine colony-forming units per mL. Scanning electron microscopy was performed before and after the biofilm formation. Results: lower amount of biofilm formation was observed in the MC group than in the CB and SLB groups (p<0.0001). SLB and CB showed similar biofilm formation rates (p=0.855). In general, the cross-sectional wires .018"x.025" showed higher biofilm formation when associated with the three types of brackets. When brackets, wires, and ligatures were associated, the sets with NiCr wires and SSL ligatures with MC brackets (p=0.0008) and CB (p=0.0003) showed higher biofilm formation. Conclusion: thus, brackets of MC with NiTi and NiCr wires showed lower biofilm formation, regardless of the ligature and cross-sectional or gauge of the wire and, MC and CB brackets with NiCr wires and SSL ligatures were more likely to accumulate biofilms (AU)

Objetivo: neste estudo, a formação de biofilme por Candida albicans em aparelhos ortodônticos fixos foi avaliada. Material e Métodos: um total de 300 bráquetes metálicos convencionais (MC), cerâmicos (CB), autoligados (SLB), com fios de níquel-titânio (NiTi) e níquel-cromo (NiCr) e tipos de ligaduras foram organizados em trinta grupos (n=10). Bráquetes, fios e ligaduras foram unidos, esterilizados, colocados em placas de 24 poços, contaminados com suspensões padronizadas de C. albicans (107 células/mL) e incubados a 37°C por 48 h para a formação de biofilmes. Os biofilmes formados foram rompidos por meio de um homogeneizador ultrassônico e suspensões foram diluídas e semeadas em ágar Sabouraud-dextrose para determinar as unidades formadoras de colônias por mL. A microscopia eletrônica de varredura foi realizada antes e após a formação do biofilme. Resultados: foi observada menor formação de biofilme no grupo MC em comparação aos grupos CB e SLB (p<0,0001). A formação de biofilme foi semelhante nos grupos SLB e CB (p=0,855). Em geral, os fios de seção transversal .018"x.025" apresentaram maior formação de biofilme quando associados aos três tipos de bráquetes. Os conjuntos com fios de NiCr e ligaduras SSL com bráquetes MC (p=0,0008) e CB (p=0,0003) apresentaram maior formação de biofilme. Conclusão: bráquetes MC com fios de NiTi e NiCr apresentaram menor formação de biofilme, independente da ligadura e secção transversal ou bitola do fio e, braquetes MC e CB com fios de NiCr e ligaduras SSL foram mais propensos a acumular biofilmes.(AU)

Estudo da técnica de Hoshi na inclinação do processo mastoide como uma característica sexual / Hoshi techniques study in the slope of the mastoid process as a sexual characteristic

Sabe-se que o processo mastoide (PM) tem sido utilizado como uma característica diferencial entre crânios de espécies diferentes e mesmo entre os sexos. Muitos são os métodos utilizados para se diferenciar os processos mastoides masculinos dos femininos. Tal diferenciação é muito importante na antropologia física e na forense. Neste trabalho foi utilizada a metodologia empregada por Hoshi (1962) na qual classifica os PM segundo a sua curvatura. O objetivo deste trabalho foi verificar a aplicabilidade desta metodologia. Foram utilizados 305 crânios humanos identificados segundo o sexo, grupo étnico e idade, pertencentes ao Museu de Crânios da Disciplina de Anatomia Descriptiva e Topográfica da Escola Paulista de Medicina - UNIFESP. Os resultados obtidos foram submetidos à análise estatística (análise descritiva e teste quiquadrado) e constatou-se que as inclinações do tipo M (70,87%) são estatisticamente típicas do sexo masculino, enquanto as inclinações do tipo N (47,47%) e F (36,36%) são encontradas nos crânios femininos, sendo suas incidências estatisticamente significativas, demonstrando que as inclinações dos tipos N e F caracterizam, na amostra em questão, os crânios femininos. Os resultados alcançados demonstraram que o método desenvolvido por Hoshi é aplicável e de fácil execução(AU)

It is known that the mastoid process (PM) has been used as a differential feature among skulls of different species and even between the sexes. Many are the methods used to differentiate the male mastoids from the female ones. Such a difference is very important in physical anthropology and forensics. In this work we use the methodology used by Hoshi (1962) in which it classifies PM according to its curvature. The objective of this work was to verify the applicability of this methodology. 305 skulls were used, identified by sex, ethnic group and age, belonging to the Museum of Skulls of the Discipline of Anatomy at the Paulista School of Medicine - UNIFESP. The results obtained were submitted to statistical analysis (descriptive analysis and chi-square test) and it was verified that the M-type inclinations (70.87%) are statistically typical of the male sex, while the N-type inclinations (47.47%) and F (36.36% ) are found in female skulls, and their incidence is statistically significant, demonstrating that the N and F inclinations characterize female skulls in the sample. The results showed that the method developed by Hoshi is applicable and easy to implement(AU)

Percepção dos pacientes em uso de enxaguatórios bucais: óleos essenciais e cloreto de cetilperidíneo / Perception of patients with use mouthwashes: essential oils and cetylpyridinium chloride

Objetivo: Avaliar a percepção dos pacientes quanto às sensações após o uso de enxagua­tórios à base de óleos essenciais e àlcool e à base de cloreto de cetilperidíneo. Métodos: Foram constituídos 2 grupos (n= 15) que realizaram 2 bochechos diários durante 3 semanas: G 1 - enxaguatório a base de óleos essenciais e álcool, em bochechos de 30 segundos; G2 - enxaguatório à base de cloreto de cetilperidínio, em bochechos de 60 segundos. Ao final de 3 semanas, os participantes responderam a um questionário sobre desconforto, alteração de paladar, sensibi­lidade dentária e ressecamento da mucosa oral. Resultados: O desconforto foi de 93,3% para G1 e de 26,6% em G2, sendo o ardor o mais relatado (86,6% em G1 e 26,6% em G2). Não foi relatada sensibilidade ou alteração dentária nos grupos avaliados. O ressecamento da mucosa ocorreu em 66,6% no G 1 e 33,3% no G2. A alteração de paladar ocorreu somente em 13,3% do G1 com diminuição da sensibilidade e gosto metálico. Conclusão: A utilização contínua de enxaguatórios com óleos essenciais e álcool causou maior desconforto quando comparado ao cloreto de cetilperidínio.

Objective: Evaluate the perceptions of patients about the effects of the use of mouthwash based on essential oils and alcohol -based and cetylpyridinium chloride. Methods: 2 groups ( n = 15) who underwent 2 mouthwash daily for 3 weeks were formed: G1 - a mouthwash based on essential oils and alcohol in mouthwash for 30 seconds; G2 - the base of mouthwash cetil­ peridínio chloride in mouthwashes 60 seconds. At the end of 3 weeks, participants answered a questionnaire on discomfort , change in taste, tooth sensitivity and dryness of the oral mucosa. Results: The discomfort was 93.3 % for G1 and 26.6% in G2, with the ardor as reported (86.6% in G1 and 26.6 % in G2) . It was reported tooth sensitivity or change in study groups. The dryness of the mucosa occurred in 66.6 % in G1 and 33.3% in G2. The taste change occurred in only 13.3% of G 1 with decreased sensitivity and metallic taste . Conclusion: The continuous use of mouthwashes with essential oils and alcohol caused greater discomfort when compared to cetilperidínio chloride.