ABSTRACT
The article presents analysis of results of microbiological study of pathologic samples from fistula and surgical wounds of 155 patients with chronic osteomyelitis of long bones in the period of exacerbation of disease during 2014-2015. In totality, 126 samples from fistula and 95 samples from wounds were analyzed. Correspondingly, 164 and 102 strains of bacteria were separated. The microbial contamination of fistula and wounds was established. The species composition of microflora was analyzed. It is established that in the analyzed groups the main agent still continues to be staphylococcus and priority pathogen Staphylococcus aureus differing slightly in rate of occurrence and coefficient of resistance. MRSA was separated up to 5.6% more often from surgical wounds than from fistula. The highest detection rate of strains S. aureus and MRSA falls on 2009-2010. In 2015, the lowest detection rate of S. aureus was marked with relatively high rate of detection of MRSA. The disk diffusion D-test was applied to determine resistance of inducible type to Clindamycin of 17 strains of S. aureus resistant to Erythromycin and sensitive to Clindamycin. The inducible resistance to Clindamycin for strains from surgery pounds made up to 62.5% that is three times higher than in case of strains separated from fistula. The important role in development of chronic of osteomyelitis of long bones is played by Gram-negative microorganisms (E. coli, Enterobacter, P. aeruginosa, etc.). The rate of detection of these microorganisms in surgery wounds makes up to 22.5% and 17.1% in fistula.
Subject(s)
Fistula/microbiology , Osteomyelitis/microbiology , Pseudomonas aeruginosa/isolation & purification , Surgical Wound/microbiology , Adult , Aged , Anti-Bacterial Agents/therapeutic use , Bone and Bones/microbiology , Bone and Bones/pathology , Clindamycin/therapeutic use , Drug Resistance, Bacterial/drug effects , Escherichia coli/drug effects , Escherichia coli/isolation & purification , Escherichia coli/pathogenicity , Female , Fistula/drug therapy , Fistula/physiopathology , Humans , Male , Methicillin-Resistant Staphylococcus aureus/drug effects , Methicillin-Resistant Staphylococcus aureus/isolation & purification , Methicillin-Resistant Staphylococcus aureus/pathogenicity , Microbial Sensitivity Tests , Middle Aged , Osteomyelitis/drug therapy , Osteomyelitis/physiopathology , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/pathogenicity , Staphylococcal Infections/drug therapy , Staphylococcal Infections/microbiology , Staphylococcal Infections/pathology , Surgical Wound/drug therapy , Surgical Wound/physiopathologyABSTRACT
The article presents the results of study of adhesive characteristics of 67 clinical strains of bacteria extracted from patients with infected endoprotheses of large joints. All analyzed strains of microorganisms had adhesive characteristics. The unequal distribution by degree of adhesiveness for analyzed groups is marked. The capability to adhesiveness in Gram-negative microorganisms is higher in comparison with Gram-positive microorganisms. The Gram-negative microorganisms form biofilms more actively.