ABSTRACT
Billions of populations are suffering from the supply-demand imbalance of clean water, resulting in a global sustainability crisis. Membrane desalination is a promising method to produce fresh water from saline waters. However, conventional membranes often encounter challenges related to low water permeation, negatively impacting energy efficiency and water productivity. Herein, we achieve ultrafast desalination over the newly developed alkadiyne-pyrene conjugated frameworks membrane supported on a porous copper hollow fiber. With membrane distillation, the membrane exhibits nearly complete NaCl rejection (>99.9%) and ultrahigh fluxes (â¼500 L m-2 h-1) from the seawater salinity-level NaCl solutions, which surpass the commercial polymeric membranes with at least 1 order of magnitude higher permeability. Experimental and theoretical investigations suggest that the large aspect ratio of membrane pores and the high evaporation area contribute to the high flux, and the graphene-like hydrophobic surface of conjugated frameworks exhibits complete salt exclusion. The simulations also confirm that the intraplanar pores of frameworks are impermeable for water and ions.
ABSTRACT
OBJECTIVES: This study was designed to evaluate the effects of cord blood mononuclear cell transplantation in multiple system atrophy (MSA). CLINICAL PRESENTATION AND INTERVENTION: Cord blood mononuclear cells (1-2 × 10(8) cells/6 ml) were injected into the subarachnoid space using lumbar puncture in patients 1 and 2 and cisterna magna puncture in patient 3 in the 3 patients with MSA. The cord blood mononuclear cell transplantation was repeated 30 days after the first treatment in patients 1 and 2; it was repeated twice in patient 3. The clinical outcomes of treatment were used to assess the Unified Multiple System Atrophy Rating Scale (UMSARS) before, 90 and 180 days after the cell transplantation. There were no clinically noticeable side effects from the cord blood mononuclear cells. The UMSARS scores improved after 90 days of the cord blood mononuclear cell therapy in all 3 patients, the most significant improvement being that in urinary incontinence and ability to walk. CONCLUSIONS: Cord blood mononuclear cell transplantation was safe and potentially effective in the treatment of MSA in the 3 patients.
Subject(s)
Cell Transplantation/methods , Fetal Blood , Leukocytes, Mononuclear/transplantation , Multiple System Atrophy/therapy , Female , Humans , Male , Middle Aged , Multiple System Atrophy/blood , Spinal PunctureABSTRACT
A supported graphene oxide membrane is endowed with selective function for olefins by a cation intercalation method. The metal-cation fixed GO membrane exhibits a high propane to propylene ideal selectivity of 1817 for single gas and a separation factor of 7.1 for binary mixtures with fast gas permeance in the order of 10-7 mol m-2 s-1 Pa-1 and reliable permeation stability.
ABSTRACT
A novel macrocyclic lactone (1) was isolated from the fermentation broth of Streptomycesmicroflavus neau3, and the structure was elucidated by extensive spectroscopic analysis. Compound 1 showed high acaricidal activity against adult mites (IC(50)=11.1 µg mL(-1)), and nematocidal activity against Caenorhabditis elegans (IC(50)=17.4 µg mL(-1)), especially the acaricidal activity against mite eggs with an IC(50) of 37.1 µg mL(-1), which was relative higher than that of the commercial acaricide and nematocide milbemycins A(3)/A(4).
Subject(s)
Acaricides/pharmacology , Antinematodal Agents/pharmacology , Caenorhabditis elegans/drug effects , Lactones/pharmacology , Macrocyclic Compounds/pharmacology , Mites/drug effects , Acaricides/chemistry , Acaricides/isolation & purification , Animals , Antinematodal Agents/chemistry , Antinematodal Agents/isolation & purification , Dose-Response Relationship, Drug , Drug Evaluation, Preclinical , Fermentation , Insecticides/chemistry , Insecticides/isolation & purification , Insecticides/pharmacology , Lactones/chemistry , Lactones/isolation & purification , Macrocyclic Compounds/chemistry , Macrocyclic Compounds/isolation & purification , Molecular Conformation , Stereoisomerism , Streptomyces/chemistry , Streptomyces/metabolism , Structure-Activity RelationshipABSTRACT
A new quinoline derivative, methyl 8-(3-methoxy-3-methylbutyl)-2-methylquinoline-4-carboxylate (1), was isolated from the endophytic strain Streptomyces sp. neau50, and the structure was elucidated by extensive spectroscopic analysis. Compound 1 showed cytotoxicity against human lung adenocarcinoma cell line A549 with an IC(50) value of 29.3 µg mL(-1).
Subject(s)
Antineoplastic Agents/chemistry , Quinolines/chemistry , Streptomyces/chemistry , Antineoplastic Agents/isolation & purification , Antineoplastic Agents/toxicity , Cell Line, Tumor , Humans , Magnetic Resonance Spectroscopy , Molecular Conformation , Quinolines/isolation & purification , Quinolines/toxicityABSTRACT
Chilli ringspot virus (ChiRSV), a novel potyvirus, was recently found in Hainan, China with high prevalence. The genomic sequence of the ChiRSV-HN/14 isolate was determined by sequencing overlapping cDNA segments generated by reverse transcription polymerase chain reaction with degenerate and/or specific primers. ChiRSV genome (GenBank Acc. no. JN008909) comprised of 9,571 nucleotides (nt) excluding the 3'-terminal poly (A) tail and contained a large open reading frame of 9,240 nt encoding a large polyprotein of 3,079 amino acids with predicted Mr of 349.1 kDa. A small, overlapping PIPO coding region was also found to span from nt 2,913 to 3,095, with a capacity to encode a peptide of 60 amino acids. ChiRSV shares sequence identities of only 48.5-65.4 and 42.9-68.7% with closely related potyviruses at the nucleotide and the amino acid levels, respectively. Phylogenetic analysis of the genomic sequences provided further evidence that ChiRSV is a distinct species of the Potyvirus genus. ChiRSV-HN/14 is most closely related to Tobacco vein banding mosaic virus and two other pepper-infecting potyviruses.
Subject(s)
Capsicum/virology , Genome, Viral , Plant Diseases/virology , Potyvirus/genetics , Potyvirus/isolation & purification , Base Sequence , Molecular Sequence Data , Phylogeny , Potyvirus/classificationABSTRACT
Transition metal oxides are the potential catalysts to replace noble-metal based catalyst for the catalytic combustion of methane due to the tolerable reactivity and low cost. However, these catalysts are challenged by the low temperature reactivity. Herein, the surface defective Co3O4 nanoplates are realized through a facile co-precipitation and thermal reduction method with the association of GO. The resultant catalysts (CoGO50) demonstrate a superior low-temperature reactivity for the methane oxidation to CO2 and H2O in comparison with the common Co3O4 catalyst. The reliable stability of CoGO50 catalyst was proved by 80 h testing with intermittent feeding of water vapor. The experimental analysis demonstrates that the presence of a small amount of GO significantly affects the catalysts in surface valence state, active oxygen species and surface oxygen vacancies through reacting with the cobalt oxide as a reductant. Moreover, GO plays as 2D confine template to form smaller and thinner nanoplates. This work provides a facile method to control the surface properties of catalyst not only for Co3O4 based catalysts but also for wider solid catalysts.
ABSTRACT
The layered graphene membrane has high potential for efficient desalination owing to its frictionless surface and hydrophobic nature. However, it has not been demonstrated so far due to the challenges related to controlling membrane microstructure. Herein, we develop a facile and simple thiol-ene click method to prepare a perfluoro-alkyl grafted graphene (fGraphene) membrane on porous ceramic, which features an ultrahigh antiwetting surface, oriented mesoporous surface entrances, and a well-defined interlamellar spacing of â¼1.1 nm. With vacuum membrane distillation, the fGraphene membranes post â¼100% rejections to the small ions of seawater, at least 1 order of magnitude higher water fluxes than those of commercial membranes and graphene-oxide-based membranes, as well as robust stability in the desalination. Fast NaCl desalinations on the fGraphene membrane were also confirmed by the reverse/forward osmosis tests. The complete rejection of ions and high flux are attributed to the interfacial sieving effect over the 2D nanochannels as well as the vapor-phase transport in the mesoscale channels, which is fundamentally different from the solution-diffusion mechanism of dense polymeric membranes and the size-sieving mechanism of microporous membranes. This work not only demonstrates a special separation effect for complete desalination over the layered graphene-based membrane but also offers a reliable method to functionalize and structure graphene membranes for other potential applications.
ABSTRACT
Urea methanolysis is a green alternative to synthesize dimethyl carbonate (UM-to-DMC). However, it is strongly challenged by the generated NH3 induced thermodynamic equilibrium limitation and the azeotropic products' separation. Herein, these predicaments are well-relieved by introducing membranes in both reaction and product separation. An NH3 permselective membrane reactor (MR) based on modified SAPO-34 membrane is successfully realized for UM-to-DMC. The permselectivity and acidity of the SAPO-34 membrane are significantly adjusted to cater the strict molecular sieving of NH3/methanol and chemical inertness upon the reaction. The MR exhibits excellent reactant conversion and DMC selectivity, resulting in >139% higher DMC yield than that of the nonmembrane reactor, due to in situ removal of NH3 by the membrane. The MR also demonstrates reliable chemical, thermal, and mechanical stability during >2000 h. Moreover, the regular SAPO-34 membrane with controlled thickness presents remarkable separation performance for the methanol-DMC azeotrope, in which the methanol-DMC separation factors and the methanol permeance are 1-2 orders of magnitude higher than those of the polymeric membranes. This study suggests the great potential that integration of such membranes offers for process intensification, energy savings, and efficiency improvement in a series of urea alcoholysis and even other NH3 releasing reactions.
ABSTRACT
The poor mechanical strength of graphene oxide (GO) membranes, caused by the weak interlamellar interactions, poses a critical challenge for any practical application. In addition, intrinsic but large-sized 2D channels of stacked GO membranes lead to low selectivity for small molecules. To address the mechanical strength and 2D channel size control, thiourea covalent-linked graphene oxide framework (TU-GOF) membranes on porous ceramics are developed through a facile hydrothermal self-assembly synthesis. With this strategy, thiourea-bridged GO laminates periodically through the dehydration condensation reactions via NH2 and/or SH with OCOH as well as the nucleophilic addition reactions of NH2 to COC, leading to narrowed and structurally well-defined 2D channels due to the small dimension of the covalent TU-link and the deoxygenated processes. The resultant TU-GOF/ceramic composite membranes feature excellent sieving capabilities for small species, leading to high hydrogen permselectivities and nearly complete rejections for methanol and small ions in gas, solvent, and saline water separations. Moreover, the covalent bonding formed at the GO/support and GO/GO interfaces endows the composite membrane with significantly enhanced stability.
ABSTRACT
INTRODUCTION: Although functional recovery and survival after ischemic infarction seem to improve in patients with prior transient ischemic attack (TIA), little is known about the role of characteristics of prior TIA in subsequent cerebral infarction. Thus, the objective of this study was to explore how the characteristics of prior TIA have a neuroprotective effect on patients with ischemic stroke. MATERIAL AND METHODS: A total of 221 patients admitted consecutively to a primary care center for first-ever ischemic stroke were divided into two groups on the basis of the presence or absence of prior TIAs. The initial NIHSS modified Rankin Scale was used to measure the severity and disability after the stroke. Subgroups were based on the TIA duration (< 10 min, 10 to 60 min, and > 60 min), TIA frequency (1 time, 2-3 times, more than 3 times), and the interval of stroke (< 1 week, 1-4 weeks, > 4 weeks). The severity of the neurologic picture on admission and functional disability after stroke were compared between patients with and without TIAs and subgroups as well. RESULTS: A total of 132 (59.73%) of the 221 patients had prior TIAs before stroke. Risk factors and the initial clinical picture did not differ between patients with or without TIAs. Patients with prior TIA had a more favorable outcome than those without TIA (59.09% vs. 43.82%), and a significant difference between the two groups was observed (χ² = 4.976, p = 0.026). Furthermore, neurological outcome in patients with prior TIA lasting for 60 min, less than 3 times and shorter intervals within 4 weeks was significantly different from that in the non-TIA group (p < 0.05). CONCLUSIONS: Prior transient ischemic attacks may have a neuroprotective effect on the subsequent ischemic stroke, and this effect might be affected by the characteristics of TIAs. Patients with TIAs of low frequency, short duration and short interval are considered to have better neurological outcomes.
ABSTRACT
This study investigated the neuroprotective effects of (2R,3S)-pinobanksin-3-cinnamate (PNC) in rats with occlusion-damaged bilateral common carotid arteries. Administration with PNC (5 and 10 mg/kg/day) for 5 weeks significantly improved the behavioral performance of rats with vascular dementia, as showed in the Morris water maze test by shortening the escape latency and latency of crossing, completing more platform crossings, as well as spending more time in the target zone. Further evaluations found that PNC could markedly decrease malondialdehyde levels, enhance superoxide dismutase activity and glutathione levels, and decrease the release of cytochrome c as well as the activities of caspases. Moreover, PNC increased Nrf2 and anti-apoptotic bcl-2 protein expression, while Nox1 and pro-apopotic bax protein expression was decreased. PNC may exert its neuroprotective effects through counteracting oxidative stress and has the potential to treat vascular dementia.
Subject(s)
Cholestenones/pharmacology , Cinnamates/pharmacology , Dementia, Vascular/drug therapy , Neuroprotective Agents/pharmacology , Oxidative Stress/drug effects , Animals , Apoptosis/drug effects , Apoptosis Regulatory Proteins/metabolism , Cognition/drug effects , Dementia, Vascular/metabolism , Drug Evaluation, Preclinical , Glutathione/metabolism , Hippocampus/drug effects , Hippocampus/metabolism , Male , Malondialdehyde/metabolism , Rats, Wistar , Superoxide Dismutase/metabolismABSTRACT
We study the problem of image denoising where images are assumed to be samples from low dimensional (sub)manifolds. We propose the algorithm of locally linear denoising. The algorithm approximates manifolds with locally linear patches by constructing nearest neighbor graphs. Each image is then locally denoised within its neighborhoods. A global optimal denoising result is then identified by aligning those local estimates. The algorithm has a closed-form solution that is efficient to compute. We evaluated and compared the algorithm to alternative methods on two image data sets. We demonstrated the effectiveness of the proposed algorithm, which yields visually appealing denoising results, incurs smaller reconstruction errors and results in lower error rates when the denoised data are used in supervised learning tasks.