ABSTRACT
There will be a continuing need for well characterized panels of EBV-transformed lymphoblastoid cell lines. Selection of the 4AOH panel was based on prior MHC typing and was intended to ensure representation of ancestral haplotypes from various racial groups. Cells from nonhuman primates, bone marrow donor-recipient pairs, and patients with IDDM were included. Selected cells from the 10IHW were included to enable further characterization. Cells were distributed to participants in the 4AOHW and were typed at multiple loci by a variety of procedures. Non-HLA genes such as TNF were included. Since the cells were distributed "blind" with hidden replicates, it was possible to evaluate the quality of the typing data. An approach to data management is described. The best current estimates of the typing of these cells are presented. The panel will be useful since it provides standards for most alleles at most loci. Since the cells are so well characterized, they represent a useful resource for MHC sequencing and for the evaluation of new typing procedures.
Subject(s)
Cell Line, Transformed/classification , Cell Line, Transformed/immunology , Databases, Factual , Histocompatibility Testing , Alleles , Animals , Asia/ethnology , Cell Transformation, Viral , Demography , HLA Antigens/genetics , Haplotypes , Herpesvirus 4, Human , Humans , Lymphocyte Activation , Pacific Islands/ethnologyABSTRACT
POLYHIPE polymers represent a novel immobilization support possessing many desirable characteristics. These materials have been used to immobilize Citrobacter freundii in order to develop a continuous bioconversion system for the anaerobic production of trimethylene glycol from glycerol. Several immobilization methods were employed, of which the most successful involved culturing the organism with POLYHIPE in shake-flasks, transferring the contents into a column, and growing the cells in situ within the column. Cell activity was determined by the amount of TMG produced from a buffered glycerol solution. The latter was passed through the columns by either a recirculation system or the superior single-pass method. The optimal system was scaled up and showed potential for further large-scale investigations. Cell lifetime in the column could be improved by supplying the cells with diluted growth medium in place of the buffer.
Subject(s)
Citrobacter/metabolism , Glycerol/metabolism , Bacteriological Techniques , Biotransformation , Culture Media , Industrial Microbiology/methods , PolystyrenesABSTRACT
Estimation of the infectivity of the agent of guinea-pig inclusion conjunctivitis for irradiated McCoy cells, assayed as inclusion-forming units, was influenced by the age of cells after irradiation, the maturation time of the inclusions, the centrifugal force and the centrifugation temperature. Agent passaged through irradiated McCoy cells or guinea-pig conjunctivae showed a greater capacity to infect irradiated McCoy cells without centrifuging than agent grown in a chick embryo. The nature of the change and the mechanism of infectivity enhancement by centrifuging are discussed.