ABSTRACT
As a critical step during innate response, the cytoplasmic ß subunit (IFN-γR2) of interferon-γ receptor (IFN-γR) is induced and translocates to plasma membrane to join α subunit to form functional IFN-γR to mediate IFN-γ signaling. However, the mechanism driving membrane translocation and its significance remain largely unknown. We found, unexpectedly, that mice deficient in E-selectin, an endothelial cell-specific adhesion molecule, displayed impaired innate activation of macrophages upon Listeria monocytogenes infection yet had increased circulating IFN-γ. Inflammatory macrophages from E-selectin-deficient mice had less surface IFN-γR2 and impaired IFN-γ signaling. BTK elicited by extrinsic E-selectin engagement phosphorylates cytoplasmic IFN-γR2, facilitating EFhd2 binding and promoting IFN-γR2 trafficking from Golgi to cell membrane. Our findings demonstrate that membrane translocation of cytoplasmic IFN-γR2 is required to activate macrophage innate response against intracellular bacterial infection, identifying the assembly of functional cytokine receptors on cell membrane as an important layer in innate activation and cytokine signaling.
Subject(s)
E-Selectin/metabolism , Immunity, Innate , Receptors, Interferon/metabolism , Animals , Calcium-Binding Proteins/antagonists & inhibitors , Calcium-Binding Proteins/genetics , Calcium-Binding Proteins/metabolism , Cell Membrane/metabolism , E-Selectin/deficiency , E-Selectin/genetics , Golgi Apparatus/metabolism , Interferon-gamma/blood , Interferon-gamma/metabolism , Listeria/pathogenicity , Macrophage Activation , Macrophages/cytology , Macrophages/immunology , Macrophages/metabolism , Mice , Mice, Inbred C57BL , Mice, Knockout , Phosphorylation , Protein Transport , RAW 264.7 Cells , RNA Interference , RNA, Small Interfering/metabolism , Receptors, Interferon/deficiency , Receptors, Interferon/genetics , Signal Transduction , Interferon gamma ReceptorABSTRACT
Identifying tumor-induced leukocyte subsets and their derived circulating factors has been instrumental in understanding cancer as a systemic disease. Nevertheless, how primary tumor-induced non-leukocyte populations in distal organs contribute to systemic spread remains poorly defined. Here, we report one population of tumor-inducible, erythroblast-like cells (Ter-cells) deriving from megakaryocyte-erythroid progenitor cells with a unique Ter-119+CD45-CD71+ phenotype. Ter-cells are enriched in the enlarged spleen of hosts bearing advanced tumors and facilitate tumor progression by secreting neurotrophic factor artemin into the blood. Transforming growth factor ß (TGF-ß) and Smad3 activation are important in Ter-cell generation. In vivo blockade of Ter-cell-derived artemin inhibits hepatocellular carcinoma (HCC) growth, and artemin deficiency abolishes Ter-cells' tumor-promoting ability. We confirm the presence of splenic artemin-positive Ter-cells in human HCC patients and show that significantly elevated serum artemin correlates with poor prognosis. We propose that Ter-cells and the secreted artemin play important roles in cancer progression with prognostic and therapeutic implications.
Subject(s)
Disease Progression , Erythroblasts/cytology , Nerve Tissue Proteins/blood , Spleen/cytology , Transforming Growth Factor beta/metabolism , Animals , Apoptosis , Carcinoma, Hepatocellular/metabolism , Cell Movement , Cell Proliferation , Epithelial-Mesenchymal Transition , Gene Expression Regulation, Neoplastic , Glial Cell Line-Derived Neurotrophic Factor Receptors/metabolism , Hep G2 Cells , Humans , Leukocyte Common Antigens/metabolism , Leukocytes/cytology , Liver Neoplasms/metabolism , Mice , Mice, Inbred C57BL , Mice, Knockout , Neoplasm Invasiveness/genetics , Signal TransductionABSTRACT
Anaplastic thyroid cancer (ATC) is a rare but highly aggressive kind of thyroid cancer. Various therapeutic methods have been considered for the treatment of ATC, but its prognosis remains poor. With the advent of the nanomedicine era, the use of nanotechnology has been introduced in the treatment of various cancers and has shown great potential and broad prospects in ATC treatment. The current review meticulously describes and summarizes the research progress of various nanomedicine-based therapeutic methods of ATC, including chemotherapy, differentiation therapy, radioiodine therapy, gene therapy, targeted therapy, photothermal therapy, and combination therapy. Furthermore, potential future challenges and opportunities for the currently developed nanomedicines for ATC treatment are discussed. As far as we know, there are few reviews focusing on the nanomedicine of ATC therapy, and it is believed that this review will generate widespread interest from researchers in a variety of fields to further expedite preclinical research and clinical translation of ATC nanomedicines.
Subject(s)
Thyroid Carcinoma, Anaplastic , Thyroid Neoplasms , Humans , Thyroid Carcinoma, Anaplastic/drug therapy , Thyroid Carcinoma, Anaplastic/genetics , Iodine Radioisotopes , Thyroid Neoplasms/drug therapy , Thyroid Neoplasms/genetics , Combined Modality Therapy , PrognosisABSTRACT
OBJECTIVES: To study the value of autotaxin (an autocrine motility factor) level in serum and bronchoalveolar lavage fluid (BALF) in predicting refractory Mycoplasma pneumoniae pneumonia (RMPP) in children and its correlation with interleukin-6 (IL-6), interleukin-8 (IL-8), and C-reactive protein (CRP). METHODS: A retrospective analysis was performed on 238 children with Mycoplasma pneumoniae pneumonia who were admitted from January 2019 to December 2021. According to disease severity, they were divided into two groups: RMPP (n=82) and general Mycoplasma pneumoniae pneumonia (GMPP; n=156). The two groups were compared in terms of the levels of autotaxin, IL-6, IL-8, and CRP in serum and BALF to study the value of autotaxin level in serum and BALF in predicting RMPP in children, as well as the correlation of autotaxin level with IL-6, IL-8, and CRP in children with RMPP. RESULTS: Compared with the GMPP group, the RMPP group had significantly higher levels of autotaxin, IL-6, IL-8, and CRP in serum and BALF (P<0.05). For the children with RMPP, the levels of autotaxin, IL-6, IL-8, and CRP in serum and BALF in the acute stage were significantly higher than those in the convalescent stage (P<0.05). The receiver operating characteristic (ROC) curve showed that the level of autotaxin in serum and BALF had a good value in predicting RMPP in children, with an area under the curve of 0.874 (95%CI: 0.816-0.935) and 0.862 (95%CI: 0.802-0.924), respectively. The correlation analysis showed that the level of autotaxin in serum and BALF was positively correlated with IL-6, IL-8, and CRP levels (P<0.001). CONCLUSIONS: The level of autotaxin in serum and BALF increases and is correlated with the degree of disease recovery and inflammatory cytokines in children with RMPP. Autotaxin can be used as a predictive indicator for RMPP in children.
Subject(s)
Mycoplasma pneumoniae , Pneumonia, Mycoplasma , C-Reactive Protein , Child , Cytokines , Humans , Interleukin-6 , Interleukin-8 , Pneumonia, Mycoplasma/diagnosis , Retrospective StudiesABSTRACT
Epigenetic modifiers have fundamental roles in defining unique cellular identity through the establishment and maintenance of lineage-specific chromatin and methylation status. Several DNA modifications such as 5-hydroxymethylcytosine (5hmC) are catalysed by the ten eleven translocation (Tet) methylcytosine dioxygenase family members, and the roles of Tet proteins in regulating chromatin architecture and gene transcription independently of DNA methylation have been gradually uncovered. However, the regulation of immunity and inflammation by Tet proteins independent of their role in modulating DNA methylation remains largely unknown. Here we show that Tet2 selectively mediates active repression of interleukin-6 (IL-6) transcription during inflammation resolution in innate myeloid cells, including dendritic cells and macrophages. Loss of Tet2 resulted in the upregulation of several inflammatory mediators, including IL-6, at late phase during the response to lipopolysaccharide challenge. Tet2-deficient mice were more susceptible to endotoxin shock and dextran-sulfate-sodium-induced colitis, displaying a more severe inflammatory phenotype and increased IL-6 production compared to wild-type mice. IκBζ, an IL-6-specific transcription factor, mediated specific targeting of Tet2 to the Il6 promoter, further indicating opposite regulatory roles of IκBζ at initial and resolution phases of inflammation. For the repression mechanism, independent of DNA methylation and hydroxymethylation, Tet2 recruited Hdac2 and repressed transcription of Il6 via histone deacetylation. We provide mechanistic evidence for the gene-specific transcription repression activity of Tet2 via histone deacetylation and for the prevention of constant transcription activation at the chromatin level for resolving inflammation.
Subject(s)
DNA-Binding Proteins/metabolism , Histone Deacetylase 2/metabolism , Inflammation/metabolism , Interleukin-6/antagonists & inhibitors , Interleukin-6/biosynthesis , Proto-Oncogene Proteins/metabolism , Acetylation , Animals , Chromatin/chemistry , Chromatin/genetics , Chromatin/metabolism , Colitis/enzymology , Colitis/immunology , Colitis/metabolism , DNA Methylation , DNA-Binding Proteins/deficiency , Dendritic Cells/cytology , Dendritic Cells/metabolism , Dioxygenases , Down-Regulation/genetics , Epigenesis, Genetic , Female , HEK293 Cells , Histones/chemistry , Histones/metabolism , Humans , I-kappa B Proteins/metabolism , Inflammation/enzymology , Inflammation/immunology , Interleukin-6/genetics , Interleukin-6/immunology , Macrophages/metabolism , Male , Mice , Mice, Inbred C57BL , Promoter Regions, Genetic/genetics , Proto-Oncogene Proteins/deficiency , Transcription, GeneticABSTRACT
In this paper, blunt slogans used in China's health campaign against coronavirus are closely examined and the public's conflicting comments on them are analyzed. These slogans, due to their extreme effectiveness in making the public comply with the health preventive measures suggested by the government, are called Yinghe or "hardcore" slogans by the Chinese people. Containing harsh and taboo language, they convey threats of death and disease, insults or negative evaluation, and harsh demands. Despite their impolite nature, "hardcore" slogans have received significantly more positive judgment than negative judgment, especially when they made their debut in rural areas in Henan, an agricultural province in China. Criticism towards these blunt slogans then gradually increased after their initial appearance. Plausible factors contributing to the change of judgment are analyzed. The public's conflicting judgment regarding the impoliteness of the slogans may be related to the public's different positioning across time and space. This study shows that impoliteness is a practice situated not only in discourse, genres, and institutions, but also in social, cultural, and political contexts. More attention should be paid to impoliteness in special social configurations (e.g., rural areas) and social emergencies, which not only contextualize a discourse event but also define it.
ABSTRACT
Toll-like receptor (TLR) signaling is critical in innate response against invading pathogens. However, the molecular mechanisms for full activation of TLR-triggered innate immunity need to be fully elucidated. The broad complex tramtrack bric-a-brac/poxvirus and zinc finger (BTB/POZ) family is a class of transcription factors involved in many biological processes. However, few BTB/POZ proteins were reported to function in innate immune response. Zinc finger and BTB domain-containing 20 (ZBTB20), a member of BTB/POZ family, functions in neurogenesis and represses α-fetoprotein gene transcription in liver. However, the immunological functions of ZBTB20 remain unknown. Here, we found that myeloid cell-specific ZBTB20 KO mice were resistant to endotoxin shock and Escherichia coli-caused sepsis. ZBTB20 deficiency attenuated TLR-triggered production of proinflammatory cytokines and type I IFN in macrophages, which attributed to higher abundance of IκBα protein and impaired activity of NF-κB. Furthermore, ChIP and next generation high-throughput DNA sequencing assay showed that ZBTB20 specifically bound to IκBα gene promoter (+1 to +60 region) after TLR activation. ZBTB20 could inhibit IκBα gene transcription, govern IκBα protein expression, and then promote NF-κB activation. Therefore, transcriptional repressor ZBTB20 is needed to promote full activation of TLR signaling and TLR-triggered innate immune response by selectively suppressing the suppressor IκBα gene transcription.
Subject(s)
I-kappa B Proteins/antagonists & inhibitors , I-kappa B Proteins/genetics , Suppression, Genetic , Toll-Like Receptors/physiology , Transcription Factors/physiology , Transcription, Genetic/immunology , Animals , Down-Regulation/genetics , Escherichia coli Infections/genetics , Escherichia coli Infections/immunology , Escherichia coli Infections/pathology , Female , I-kappa B Proteins/metabolism , Immunity, Innate/genetics , Macrophages/immunology , Macrophages/metabolism , Macrophages/pathology , Male , Mice , Mice, 129 Strain , Mice, Inbred C57BL , Mice, Knockout , Myeloid Cells/immunology , Myeloid Cells/pathology , NF-KappaB Inhibitor alpha , NF-kappa B/metabolism , Promoter Regions, Genetic , Protein Binding/genetics , Shock, Septic/genetics , Shock, Septic/immunology , Transcription Factors/deficiency , Transcription Factors/geneticsABSTRACT
Natural killer T cells (NKT cells) are effector cells, but also regulator of immune response, which either promote or suppress immune response through production of different cytokines. However, the subsets of NKT cells with definite phenotype and regulatory function need to be further identified. Furthermore, the mechanisms for NKT cells to regulate immune response remain to be fully elucidated. Here we identified CD11b(+) invariant NKT (CD11b(+) iNKT) cells as a new subset of regulatory NKT cells in mouse models with infection. αGalCer:CD1d complex(+)TCRß(+)NK1.1(+) NKT cells could be categorized to CD11b(+) and CD11b(-) subsets. NKT cells are enriched in liver. During Listeria monocytogenes infection, hepatic CD11b(+) iNKT cells were significantly induced and expanded, with peak expansion on day 8. CD11b(+) iNKT cells were also expanded significantly in spleen and mesenteric lymph nodes. As compared to CD11b(-) iNKT cells, CD11b(+) iNKT cells expressed higher levels of CD27, FasL, B7H1, CD69, and particularly higher level of membrane-bound TGF-ß1 (mTGF-ß1), but produced less IFN-γ, IL-4, IL-10 and TGF-ß1. Hepatic CD11b(+) iNKT cells suppressed antigen-nonspecific and OVA-specific CD4 and CD8 T cell proliferation through mTGF-ß1 both in vitro and in vivo, meanwhile, they did not interfere with activation of CD4 T cells and cytotoxicity of the activated CD8 T cells. Thus, we have identified a new subset of pathogen-expanded CD11b(+) invariant NKT cells which can feedback inhibit T cell response through cell-to-cell contact via cell surface (membrane-bound) TGF-ß1, especially at the late stage of immune response against infection. CD11b(+) regulatory iNKT cells may contribute to protect host from pathological injure by preventing immune overactivation.
Subject(s)
Listeria monocytogenes/immunology , Listeriosis/immunology , Liver/immunology , Membrane Proteins/metabolism , Natural Killer T-Cells/immunology , Transforming Growth Factor beta1/metabolism , Animals , CD11b Antigen/genetics , CD11b Antigen/metabolism , CD4-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/immunology , Cell Proliferation , Cells, Cultured , Cytokines/metabolism , Cytotoxicity, Immunologic , Immunosuppression Therapy , Liver/microbiology , Lymphocyte Activation , Mice , Mice, Inbred C57BL , Mice, Knockout , Mice, TransgenicABSTRACT
UNLABELLED: Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide with limited therapeutic options. HCC-induced immunosuppression often leads to ineffectiveness of immuno-promoting therapies. Currently, suppressing the suppressors has become the potential strategy for cancer immunotherapy. So, figuring out the immunosuppressive mechanisms induced and employed by HCC will be helpful to the design and application of HCC immunotherapy. Here, we identified one new subset of human CD14(+) CTLA-4(+) regulatory dendritic cells (CD14(+) DCs) in HCC patients, representing â¼13% of peripheral blood mononuclear cells. CD14(+) DCs significantly suppress T-cell response in vitro through interleukin (IL)-10 and indoleamine-2,3-dioxygenase (IDO). Unexpectedly, CD14(+) DCs expressed high levels of cytotoxic T-lymphocyte antigen-4 (CTLA-4) and programmed death-1, and CTLA-4 was found to be essential to IL-10 and IDO production. So, we identified a novel human tumor-induced regulatory DC subset, which suppresses antitumor immune response through CTLA-4-dependent IL-10 and IDO production, thus indicating the important role of nonregulatory T-cell-derived CTLA-4 in tumor-immune escape or immunosuppression. CONCLUSIONS: These data outline one mechanism for HCC to induce systemic immunosuppression by expanding CD14(+) DCs, which may contribute to HCC progression. This adds new insight to the mechanism for HCC-induced immunosuppression and may also provide a previously unrecognized target of immunotherapy for HCC.
Subject(s)
CD4-Positive T-Lymphocytes/metabolism , CTLA-4 Antigen/metabolism , Carcinoma, Hepatocellular/metabolism , Dendritic Cells/metabolism , Indoleamine-Pyrrole 2,3,-Dioxygenase/metabolism , Interleukin-10/metabolism , Lipopolysaccharide Receptors/metabolism , Liver Neoplasms/metabolism , Adolescent , Adult , Aged , CD11b Antigen/metabolism , CD4-Positive T-Lymphocytes/pathology , Carcinoma, Hepatocellular/pathology , Case-Control Studies , Dendritic Cells/pathology , Disease Progression , Female , Humans , Immunosuppression Therapy , Immunotherapy , In Vitro Techniques , Leukocytes, Mononuclear/pathology , Liver Neoplasms/pathology , Male , Middle Aged , Phenotype , Programmed Cell Death 1 Receptor/metabolism , Young AdultABSTRACT
Regulatory T cells can restrict the uncontrolled immune response and inflammation, avoiding pathologic immune injury to the host and thus playing important roles in the maintenance of immune homeostasis. Until recently, many subsets of CD4 and CD8 regulatory T cells have been reported. In this study, we identified CD11c(high)CD8(+) T cells as a new subset of CD8(+) regulatory T cells. During Listeria monocytogenes and Staphylococcus aureus infection, two subsets of CD8 T cells were classified according to the expression level of CD11c, including CD11c(low)CD8(+) and CD11c(high)CD8(+) T cells. CD11c(low)CD8(+) T cells, existing during the whole period of infection, act as conventional activated T cells to kill target cells in a perforin-dependent manner. Interestingly, CD11c(high)CD8(+) T cells appeared only at a late stage of infection, expressed relatively high CD122 and low CD69, did not secrete IFN-γ, IL-10, TGF-ß, and exhibited much more potent cytotoxicity against target cells via Fas ligand-Fas pathway in an Ag-independent manner. Ligation of CD11c was important in the cytotoxicity of CD11c(high)CD8(+) T cells. Furthermore, CD11c(high)CD8(+) T cells could directly kill the activated CD4 T cells both in vitro and in vivo, whereas CD11c(low)CD8(+) T cells could not. Thus, we identified an infection-induced new subset of CD11c(high)CD8(+) regulatory T cells, which might contribute to protect host from pathological immune injure. Our results indicate that CD11c(+)CD8(+) T cells are constitute a heterogeneous population that can be divided further into regulatory CD11c(high)CD8(+) T cell subset and effector CD11c(low)CD8(+) T cell subset, thus adding insight to the role of CD8 T cells in immune response and regulation.
Subject(s)
CD4-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/immunology , Fas Ligand Protein/immunology , T-Lymphocyte Subsets/immunology , T-Lymphocytes, Regulatory/immunology , fas Receptor/immunology , Animals , CD11 Antigens/immunology , CD11 Antigens/metabolism , CD4-Positive T-Lymphocytes/metabolism , CD8-Positive T-Lymphocytes/metabolism , Cell Separation , Enzyme-Linked Immunosorbent Assay , Fas Ligand Protein/metabolism , Flow Cytometry , Lymphocyte Activation/immunology , Mice , Mice, Inbred C57BL , Signal Transduction/immunology , T-Lymphocyte Subsets/metabolism , T-Lymphocytes, Regulatory/metabolism , fas Receptor/metabolismABSTRACT
Dendritic cells (DCs) play important roles in the initiation of immune response and also in the maintenance of immune tolerance. Now, many kinds of regulatory DCs with different phenotypes have been identified to suppress immune response and contribute to the control of autoimmune diseases. However, the mechanisms by which regulatory DCs can be regulated to exert the immunosuppressive function in the immune microenvironment remain to be fully investigated. In addition, how T cells, once activated, can feedback affect the function of regulatory DCs during immune response needs to be further identified. We previously identified a unique subset of CD11b(hi)Ia(low) regulatory DCs, differentiated from mature DCs or hematopoietic stem cells under a stromal microenvironment in spleen and liver, which can negatively regulate immune response in a feedback way. Here, we show that CD11b(hi)Ia(low) regulatory DCs expressed high level of Fas, and endothelial stromal cell-derived TGF-ß could induce high expression of Fas on regulatory DCs via ERK activation. Fas ligation could promote regulatory DCs to inhibit CD4(+) T cell proliferation more significantly. Furthermore, Fas ligation preferentially induced regulatory DCs to produce IL-10 and IP-10 via ERK-mediated inactivation of GSK-3 and subsequent up-regulation of ß-catenin. Interestingly, activated T cells could promote regulatory DCs to secrete more IL-10 and IP-10 partially through FasL. Therefore, our results demonstrate that Fas signal, at least from the activated T cells, can promote the immunosuppressive function of Fas-expressing regulatory DCs, providing a new manner for the regulatory DCs to regulate adaptive immunity.
Subject(s)
Dendritic Cells/cytology , Extracellular Signal-Regulated MAP Kinases/metabolism , Signal Transduction , beta Catenin/metabolism , fas Receptor/metabolism , Active Transport, Cell Nucleus , Animals , Bone Marrow Cells/cytology , CD4-Positive T-Lymphocytes/cytology , Chemokine CXCL10/metabolism , Enzyme Activation , Glycogen Synthase Kinase 3/metabolism , Homeostasis , Immune System , Immunosuppression Therapy , Interleukin-10/metabolism , Mice , Mice, Inbred C57BLABSTRACT
NK cells are important innate immune cells with potent cytotoxicity that can be activated by type I IFN from the host once infected. How NK cell cytotoxicity is activated by type I IFN and then tightly regulated remain to be fully elucidated. MicroRNAs (miRNAs, or miRs) are important regulators of innate immune response, but the full scale of miRNome in human NK cells remains to be determined. In this study, we reported an in-depth analysis of miRNomes in resting and IFN-α-activated human NK cells, found two abundant miRNAs, miR-378 and miR-30e, markedly decreased in activated NK cells by IFN-α, and further proved that miR-378 and miR-30e directly targeted granzyme B and perforin, respectively. Thus, IFN-α activation suppresses miR-378 and miR-30e expression to release cytolytic molecule mRNAs for their protein translation and then augments NK cell cytotoxicity. Importantly, the phenomena are also confirmed in human NK cells activated by other cytokines and even in the sorted CD16(+)CD56(dim)CD69(+) human NK cell subset. Finally, miR-378 and miR-30e were proved to be suppressors of human NK cell cytotoxicity. Taken together, our results reveal that downregulated miR-378 and miR-30e during NK cell activation are negative regulators of human NK cell cytotoxicity, providing a mechanistic explanation for regulation of NK cell function by miRNAs.
Subject(s)
Cytotoxicity, Immunologic/genetics , Down-Regulation/immunology , Interferon Type I/physiology , Killer Cells, Natural/immunology , Lymphocyte Activation/immunology , MicroRNAs/metabolism , Resting Phase, Cell Cycle/immunology , Cells, Cultured , Down-Regulation/genetics , Humans , Killer Cells, Natural/cytology , Killer Cells, Natural/metabolism , Lymphocyte Activation/genetics , MicroRNAs/antagonists & inhibitors , MicroRNAs/genetics , Resting Phase, Cell Cycle/genetics , Transcriptome/geneticsABSTRACT
Memory CD8+ T cell generation is crucial for pathogen elimination and effective vaccination against infection. The cellular and molecular circuitry that underlies the generation of memory CD8+ T cells remains elusive. Eosinophils can modulate inflammatory allergic responses and interact with lymphocytes to regulate their functions in immune defense. Here we report that eosinophils are required for the generation of memory CD8+ T cells by inhibiting CD8+ T cell apoptosis. Eosinophil-deficient mice display significantly impaired memory CD8+ T cell response and weakened resistance against Listeria monocytogenes (L.m.) infection. Mechanistically, eosinophils secrete interleukin-4 (IL-4) to inhibit JNK/Caspase-3 dependent apoptosis of CD8+ T cells upon L.m. infection in vitro. Furthermore, active eosinophils are recruited into the spleen and secrete more IL-4 to suppress CD8+ T cell apoptosis during early stage of L.m. infection in vivo. Adoptive transfer of wild-type (WT) eosinophils but not IL-4-deficient eosinophils into eosinophil-deficient mice could rescue the impaired CD8+ T cell memory responses. Together, our findings suggest that eosinophil-derived IL-4 promotes the generation of CD8+ T cell memory and enhances immune defense against L.m. infection. Our study reveals a new adjuvant role of eosinophils in memory T cell generation and provides clues for enhancing the vaccine potency via targeting eosinophils and related cytokines.
Subject(s)
CD8-Positive T-Lymphocytes , Listeriosis , Mice , Animals , Listeriosis/genetics , Listeriosis/microbiology , Interleukin-4/genetics , Eosinophils , Memory T CellsABSTRACT
Bisphenol S (BPS) is an industrial chemical that is widely used to manufacture daily items, such as plastic water bottles, milk bottles, water cups, and paper products. BPS is a biologically toxic environmental endocrine disruptor. Long-term exposure to BPS can disrupt the reproductive system, endanger health, and increase the risk of cancer. The metal-organic framework UiO-66 is characterised with high thermal and chemical stability, a simple synthetic route, and low preparation cost. In this study, we modified UiO-66 with nucleic acid aptamers to prepare an 'on-off-on' fluorescent sensor for the simple and rapid detection of BPS. The FAM-labelled aptamer was selected as the fluorescent probe (i.e. 'on'). In the presence of UiO-66, the FAM-labelled aptamer adsorbed onto the surface of the UiO-66 material, and the fluorescence of FAM was quenched by photoinduced electron transfer (i.e. 'off'). When BPS was introduced into the system, the configuration of the FAM-labelled aptamer changed after binding to BPS, and the adsorption of FAM on UiO-66 weakened, resulting in fluorescence recovery (i.e. 'on'). Based on this principle, the reaction system was optimised, and the BPS content was analysed according to the change in the fluorescence signal. The signals changed linearly in the BPS concentration range of 2.0 × 10-4-4.0 × 10-2 mmol L-1, and the system had a detection limit of 1.84 × 10-4 mmol L-1. The sensor was successfully used to detect the BPS content in commercial plastic bottled water.
Subject(s)
Metal-Organic FrameworksABSTRACT
B cells have multiple functions in adaptive immunity, including antibody production, antigen presentation and regulation of T-cell responses. Recent evidences indicate that B cells have more subsets than previously thought and may have non-classical functions, such as involvement in innate immunity and immune regulation; however, how B cells respond to microbial infection and elicit innate defense remain unclear. In this study, we identified a new subset of PDCA-1(+) Siglec-H(-) CD19(+) B cells in mice during the early period of bacterial infection with Listeria monocytogenes. PDCA-1(+) Siglec-H(-) CD19(+) B cells secreted large amounts of IFN-α and thus facilitated IFN-γ production and cytotoxicity function of natural killer (NK) cells via IFN-α. B-cell-deficient Btk(-/-) mice were incapable of producing PDCA-1(+) CD19(+) B cells, and were more sensitive to L. monocytogenes infection. Adoptive transfer of PDCA-1(+) CD19(+) B cells to Btk(-/-) mice normalized their resistance to L. monocytogenes infection. Furthermore, we found that macrophages were essential for the inducible generation of PDCA-1(+) Siglec-H(-) CD19(+) B cells via CD40-CD40L ligation. Therefore, we have identified a new subset of PDCA-1(+) Siglec-H(-) CD19(+) B cells, which enhances innate immune responses against bacterial infection by activating NK cells via secretion of IFN-α.
Subject(s)
B-Lymphocyte Subsets/immunology , Immunity, Innate , Interferon-alpha/biosynthesis , Killer Cells, Natural/immunology , Adoptive Transfer , Agammaglobulinaemia Tyrosine Kinase , Animals , Antigens, CD19/metabolism , Antigens, Differentiation/metabolism , B-Lymphocyte Subsets/enzymology , Base Sequence , CD40 Antigens/deficiency , CD40 Antigens/genetics , CD40 Antigens/metabolism , CD40 Ligand/deficiency , CD40 Ligand/genetics , CD40 Ligand/metabolism , DNA Primers/genetics , In Vitro Techniques , Interferon-alpha/deficiency , Interferon-alpha/genetics , Interferon-gamma/biosynthesis , Interferon-gamma/deficiency , Interferon-gamma/genetics , Lectins/metabolism , Listeriosis/enzymology , Listeriosis/immunology , Lymphocyte Activation , Macrophages/immunology , Mice , Mice, Inbred C57BL , Mice, Knockout , Protein-Tyrosine Kinases/deficiency , Protein-Tyrosine Kinases/genetics , Receptors, Cell Surface/metabolismABSTRACT
UNLABELLED: The liver, a unique tolerogenic organ, is regarded as the site to trap and destroy aging erythrocytes and activated T cells. However, to date, the mechanisms for why the liver is tolerogenic and whether liver Kupffer cells (KC) are critical phagocytes for apoptotic cells (AC) contributing to the liver immunosuppression remain unclear. Here we report that KC is the main phagocyte for AC in the liver. Contact of AC inhibits proinflammatory cytokine but enhances anti-inflammatory cytokine production of KC in response to lipopolysaccharide (LPS) stimulation. Membrane-bound transforming growth factor (TGF)-ß on AC is responsible for the increased production of interleukin (IL)-10 in KC through extracellular signal-regulated kinase (ERK) activation via the Smad3 pathway. Importantly, KC-derived IL-10 is critical for AC infusion-mediated protection of endotoxin-induced fulminant hepatitis through suppression of tumor necrosis factor (TNF)-α and nitric oxide (NO) production from KC and consequently attenuation of KC-mediated cytolysis of hepatocytes. CONCLUSION: AC can be preferentially phagocytosed by KC in the liver, leading to attenuation of fulminant hepatitis through IL-10-mediated suppression of KC-derived inflammatory TNF-α and NO production. These findings demonstrate that priming of KC by AC may contribute to maintain liver immunosuppression, providing a new mechanistic explanation for how immune homeostasis is maintained in the liver.
Subject(s)
Hepatitis/immunology , Interleukin-10/biosynthesis , Kupffer Cells/immunology , Liver/cytology , Phagocytosis/physiology , Transforming Growth Factor beta/physiology , Animals , Apoptosis , Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors , Female , Flavonoids/pharmacology , Immune Tolerance/physiology , Interleukin-10/physiology , Kupffer Cells/metabolism , Lipopolysaccharides/toxicity , Liver/immunology , Male , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Tumor Necrosis Factor-alpha/immunologyABSTRACT
Interleukin-17A-producing T cells, especially Th17, have been shown to be involved in inflammatory autoimmune diseases and host defense against extracellular infections. However, whether and how IL-17A or IL-17A-producing cells can help protection against intracellular bacteria remains controversial, especially how it regulates the adaptive immunity besides recruitment of neutrophils in the innate immune system. By infecting IL-17A-deficient mice with Listeria monocytogenes, we show in this study that IL-17A is required for the generation of Ag-specific CD8(+) CTL response against primary infection, but not for the generation of memory CD8(+) T cells against secondary challenge. Interestingly, we identify γδT cells, but not conventional CD4(+) Th17 cells, as the main cells for innate IL-17A production during L. monocytogenes infection. Furthermore, γδT cells are found to promote Ag-specific CD8(+) T cell proliferation by enhancing cross-presentation of dendritic cells through IL-17A. Adoptive transfer of Il17a(+/+) γδT cells, but not Il17a(-/-) γδT cells or Il17a(+/+) CD4(+) T cells, were sufficient to recover dendritic cells cross-presentation and defective CD8(+) T cell response in Il17a(-/-) mice. Our findings indicate an important role of infection-inducible IL-17A-producing γδT cells and their derived IL-17A against intracellular bacterial infection, providing a mechanism of IL-17A for regulation of innate and adaptive immunity.