ABSTRACT
PQ Birch represents an allergen-specific immunotherapy for the treatment of birch pollinosis. It consists of native birch pollen extract chemically modified with glutaldehyde adsorbed to L-tyrosine in its microcrystalline form with addition of the adjuvant Monophosphoryl Lipid A (MPL®). A nonclinical safety testing strategy was designed based upon interpretation of current legislation and regulatory intelligence and comprised genotoxicity studies (bacterial reverse mutation and Chinese hamster ovary micronucleus assays), a rat repeat dose toxicology study and a rabbit local tolerance study. No safety findings of concern were found. Thus, no evidence of genotoxicity was found. Relatively minor, immunostimulatory effects were seen following repeated subcutaneous dosing (once every 2 weeks for 13 weeks) as reversible increased white cell count (notably neutrophils), increased globulin level (resulting in decreased albumin/globulin [A/G] ratio) and increased fibrinogen, as well as minor dose site reaction in the form of inflammatory cell infiltrate. These findings are likely due to the immunostimulatory nature of MPL® and/or the presence of L-tyrosine within the adjuvanted vaccine. Similar dose site inflammatory changes to the injected formulation were also noted in the rabbit local tolerance study.
Subject(s)
Adjuvants, Immunologic/toxicity , Betula/immunology , Immunotherapy/adverse effects , Lipid A/analogs & derivatives , Pollen/immunology , Tyrosine/toxicity , Animals , CHO Cells , Cricetulus , Female , Lipid A/toxicity , Male , Mutagenicity Tests , Rabbits , Rats, Wistar , Rhinitis, Allergic, Seasonal/therapy , Salmonella typhimurium/drug effects , Salmonella typhimurium/growth & development , Skin/drug effectsABSTRACT
PQ Grass represents an allergen-specific immunotherapy for pre-seasonal treatment of patients with seasonal allergic rhinitis (or rhinoconjunctivitis) with or without mild-to-moderate bronchial asthma. It consists of a native pollen extract for 13 grass species, chemically modified with glutaraldehyde, and adsorbed to l-tyrosine in a microcrystalline form with addition of the adjuvant Monophosphoryl Lipid A (MPL® ). Previous non-clinical safety testing, including rat repeat dose toxicity in adult and juvenile animals, rat reproductive toxicity and rabbit local tolerance studies showed no safety findings of concern. A new Good Laboratory Practice compliant rat subcutaneous repeat dose toxicity study to evaluate a higher clinical dose and modified posology (once every 2 weeks for 13 weeks) showed no signs of toxicity. As seen in previous studies, relatively minor, immunostimulatory effects were seen such as reversible increased white cell count (notably neutrophils), increased globulin level (resulting in decreased A/G ratio) and increased fibrinogen as well as minor dose site reaction in the form of inflammatory cell infiltrate. These findings are likely due to the immunostimulatory nature of MPL and/or the presence of l-tyrosine within the adjuvanted vaccine. This new toxicity study with PQ Grass therefore supports longer posology with higher dose levels.
Subject(s)
Adjuvants, Immunologic/toxicity , Adjuvants, Immunologic/therapeutic use , Hypersensitivity/drug therapy , Hypersensitivity/etiology , Immunotherapy/adverse effects , Immunotherapy/methods , Poaceae/adverse effects , Animals , Female , Humans , Male , Models, Animal , Rats, WistarABSTRACT
Polycyclic aromatic hydrocarbons (PAH) are ubiquitous pollutants in the atmosphere, predominantly known for their toxicity. Although there has been substantial work on the atmospheric degradation of PAH, little is known about how the presence of atmospheric droplets (e.g., a fog cloud) affects the fate of PAH. In order to assess the processing of PAH and their corresponding oxidation products during a fog event, two field-sampling campaigns in Fresno, CA and Davis, CA were conducted. The simultaneous evaluation of concentrations of the PAH and oxygenated polycyclic aromatic compounds (OPAC) in the gas phase, particulate matter and fog water droplets before, during and after fog allows for the characterization of transformative and transport processes in a fog cloud. By tracking the ratio of OPAC to PAH in the individual atmospheric phases, two major polycyclic aromatic compounds-processing pathways can be identified: (i) the dissolution of OPAC from particulate matter and (ii) the uptake and oxidation of PAH in the fog water droplets. Wet deposition steadily decreases the pollutant concentration in the fog cloud droplets during a fog event; however, uptake and concentration via evaporative water loss upon the dissipation of a fog cloud cause an increase in the atmospheric pollutant concentration.
Subject(s)
Air Pollutants/analysis , Atmosphere/chemistry , Environmental Monitoring/methods , Polycyclic Aromatic Hydrocarbons/analysis , Weather , Air Pollution/statistics & numerical data , California , CitiesABSTRACT
The occurrence, source, and sink processes of N-nitrosodimethylamine (NDMA) have been explored by means of combined laboratory, field, and model studies. Observations have shown the occurrence of NDMA in fogs and clouds at substantial concentrations (7.5-397 ng L(-1)). Laboratory studies were conducted to investigate the formation of NDMA from nitrous acid and dimethylamine in the homogeneous aqueous phase. While NDMA was produced in the aqueous phase, the low yields (<1%) observed could not explain observational concentrations. Therefore gaseous formation of NDMA with partitioning to droplets likely dominates aqueous NDMA formation. Box-model calculations confirmed the predominant contributions from gas phase formation followed by partitioning into the cloud droplets. Measurements and model calculations showed that while NDMA is eventually photolyzed, it might persist in the atmosphere for hours after sunrise and before sunset since the photolysis in the aqueous phase might be much less efficient than in the gas phase.
Subject(s)
Air Pollutants/analysis , Atmosphere/chemistry , Dimethylnitrosamine/analysis , Weather , Air Movements , Air Pollutants/chemical synthesis , Air Pollutants/chemistry , Dimethylnitrosamine/chemical synthesis , Dimethylnitrosamine/chemistry , Environmental Monitoring , Kinetics , Models, Chemical , Photolysis , Steam/analysisABSTRACT
BACKGROUND: Our knowledge of allergen structure and function continues to rise and new scientific data on the homology and cross-reactivity of allergen sources should be considered to extend the work of Lorenz et al., 2009 (Int Arch Allergy Immunol. 148(1):1-1, 2009) and the concept of homologous groups. In addition to this, sophisticated techniques such as mass spectrometry (MS) are increasingly utilised to better characterise the complex mix and nature of allergen extracts. METHODS: Homology models were used of Fag s 1 (Beech) and Cyn d 1 (Bermuda grass) and compared with template crystal structures of Bet v 1 and Phl p 1 from the 'exemplar' species of Birch and Timothy grass, respectively. ELISA experiments were performed to assess cross-reactivity of Beech (tree) and Bermuda (grass) extracts to rabbit sera raised to either "3-Tree" (Birch, Alder and Hazel) extract or "Grass" (12-grass mix extract), respectively. The comparability of biochemical stability of different allergen sources was assessed through statistical methods for a range of tree and grass species. RESULTS: Allergen cross-reactivity and/or structural homology have been described providing justification for inclusion of Beech within the Birch homologous tree group. Data from Bermuda grass (Cyn d 1) provides further justification for the inclusion of this species into the homologous group of the sweet grasses. However, further characterisation of relevant allergens from Bermuda grass and, in particular, comparison of cross-reactive patterns between subjects specifically in areas with high abundance of both Pooideae and Chloridoideae is sought. CONCLUSION: MS allows the possibility to identify individual proteins or allergens from complex mixes by mass and/or sequence, and this has been extensively applied to the allergen field. New data on the homology, cross-reactivity and biological parameters of allergen sources have been considered to extend the work of Lorenz et al., 2009 in the context of tree and grass species. The concept of homologous groups is certainly dynamic allowing the flexibility and potential in streamlining quality parameters, such as stability profiles, due to extrapolation of exemplar data to a wider range of allergens.