Prepartum plane of energy intake affects serum biomarkers for inflammation and liver function during the periparturient period.

Serum collected from multiparous Holsteins (n = 73) in a previous experiment was used to determine the effect of prepartum plane of energy intake on metabolites related to inflammation and liver function in periparturient cows. Prepartum diets were in a 3 × 2 factorial arrangement over the far-off (d -65 to -26 before expected parturition) and close-up (d -25 relative to parturition until parturition) dry periods. During the far-off period, 2 diets were fed for ad libitum intake (ADLIB) to supply either 100% (100E) or 150% (150E) of National Research Council recommendations for net energy for lactation for mature cows in late gestation. For the third dietary far-off treatment, the 150E diet was fed at restricted intake (REST) to supply 80% (80E) of recommendations. During the close-up period, cows were fed a diet either at ADLIB or REST to supply 150% of net energy for lactation or 80% of net energy for lactation requirements, respectively. Beginning at parturition, all cows were fed a lactation diet through 56 d in milk. Cows fed 150E tended to accumulate more liver lipid postpartum; peak lipid accumulation occurred in all groups on d 14 postpartum. Cows fed 150E tended to have lower serum Ca on d 1 after calving, and cows fed REST had higher Ca than those fed ADLIB. Cows fed 150E tended to have higher serum bilirubin prepartum compared with other groups. Feeding REST in the close-up period resulted in higher bilirubin prepartum compared with ADLIB; bilirubin was positively associated (r = 0.34) with lipid accumulation postpartum. Feeding REST resulted in lower serum vitamin A (an indirect measure of retinol binding protein) prepartum compared with ADLIB, especially when coupled with 80E. Postpartum paraoxonase was negatively associated (r = -0.27) and ceruloplasmin was positively associated (r = 0.21) with liver lipid accumulation postpartum. A larger spike in haptoglobin was observed on d 1 and 7 postpartum for cows fed 100E and 150E during the far-off period followed by REST during the close-up period. The ratio of albumin to globulin in serum was higher for cows fed 100E than for those fed 150E both prepartum and postpartum. Liver activity index indicated poorer liver function for cows fed 150E in the far-off period regardless of close-up diet. Negative liver function was also noted for cows fed 80E and REST. Cows in the 100E group fed ADLIB or REST had positive liver activity index postpartum. Avoidance of gross overconsumption of energy prepartum, particularly during the far-off period, appears best to reduce systemic inflammatory signals and improve liver function.

Prepartum dietary energy intake alters hepatic expression of genes related to peroxisome proliferator-activated receptor and inflammation in peripartal dairy cows.

We determined the effect of prepartum plane of energy intake on liver function and metabolism pre- and postpartum by combining in vivo and in vitro data with mRNA expression data. A subset of multiparous prepartal Holsteins (n = 18) from a previously conducted experiment consumed 1 of 3 amounts of dietary energy intake, relative to their requirements. A diet formulated to allow consumption of ≥150% of net energy requirements during the far-off dry period and the close-up dry period was fed for ad libitum intake (150E) or in restricted amounts so that cows consumed 80% of requirements for energy (80E). A second diet was formulated to include wheat straw (26.1% of dry matter) to limit energy intake to 100% of NRC (2001) requirements for energy when fed ad libitum during the far-off period (100E). In the close-up period, 100E was fed the 150E diet for ad libitum intake. Expression of mRNA for genes related to fatty acid oxidation (PPARA, CPT1A, ACOX1) was greater for 100E cows than 150E cows on d 14 postpartum. These expression patterns were related to in vitro data for conversion of palmitate to CO2, acid-soluble products, and esterified products by liver slices. Abundance of mRNA for PC displayed a sharp peak for all groups on d 1 postpartum, but serum glucose did not reflect this peak. The mRNA expression of SREBF1 was greater for 150E and 100E cows prepartum compared with 80E, and was positively related to rate of palmitate esterification postpartum. Expression of NR1H3 (LXRA) mRNA was greater for 100E cows on d 14 postpartum compared with 150E cows, which corresponded to expression of PPARA. An inflammatory response occurred in the liver around the time of parturition for 150E cows, as expression of IL1B was elevated both pre- and postpartum compared with 100E cows. The spike in IL1B expression for 150E cows on d 14 postpartum corresponded to the peak concentration of total lipids in liver tissue for all groups in this experiment. Overconsumption of energy prepartum was detrimental to the expression of important genes related to PPAR and liver function, especially postpartum. Furthermore, results provide evidence for inflammation related to accumulation of lipids in liver and overnutrition prepartum.

Influence of starter crude protein content on growth and body composition of dairy calves in an enhanced early nutrition program.

Our objectives were to determine the effect of starter crude protein (CP) content on body composition of male Holstein calves from birth to 10 wk of age in an enhanced early nutrition program, and to compare the enhanced program to a conventional milk replacer program. Calves (n = 45) were purchased on the day of birth and assigned to a randomized block design. Eight calves were harvested at baseline and remaining calves were divided among the following 3 dietary treatments: (1) low rate of milk replacer [LMR; 20.6% CP, 21.7% fat; 1.25% of body weight (BW) as dry matter (DM)] plus conventional starter (CCS; 21.5% CP, DM basis); n = 11 calves; (2) high rate of milk replacer (HMR; 29.1% CP, 17.3% fat; 1.5% of BW as DM for wk 1, 2% of BW as DM wk 2-5, 1% of BW as DM wk 6) plus conventional starter; n = 12 calves; and (3) enhanced milk replacer (HMR) plus high-CP starter (HCS; 26% CP, DM basis); n = 14 calves. A subset of calves (n = 8) was harvested on d 2 to provide baseline data. Calves began treatments on d 2 or 3 of age. Calves were weaned at d 42. Starter was available ad libitum. Calves from each treatment were harvested at 5 (n = 18) and 10 (n = 19) wk of age and divided into 4 fractions: carcass; viscera; blood; and head, hide, feet, and tail. Fractions were analyzed for energy, CP, lipid, and ash. Average weekly starter intake did not differ between enhanced treatments. Gain of BW was greater for calves fed HMR than for LMR, but was unaffected by starter CP. Carcass weights at 5 wk were greater for HMR but did not differ between starter CP content. At 10 wk, carcass weights were heavier for HMR and had a greater percentage of empty BW for HMR + CCS than for HMR + HCS. At 10 wk, the weights of reticulorumen and liver were greater for calves fed HMR + HCS than for those fed HMR + CCS. At 5 wk, empty BW gain for HMR contained more water and less fat and ash than in calves fed LMR. At 10 wk, empty BW gain for calves fed HMR + HCS contained a greater percentage of water and less fat than for calves fed HMR + CCS. Plasma ß-hydroxybutyrate was greater after weaning for calves fed HMR + HCS than for those fed HMR + CCS. After weaning, calves fed HMR had greater plasma total protein concentration than those fed LMR, and total protein was greater for calves fed HMR + HCS than those fed HMR + CCS. Plasma urea N was greater for calves fed HMR treatments, and postweaning was greater for calves fed HMR + HCS. A high-CP starter had minimal effect on empty BW gain before weaning, but after weaning it tended to increase mass of reticulorumen and liver.

Comparison of prepartum low-energy or high-energy diets with a 2-diet far-off and close-up strategy for multiparous and primiparous cows.

Previous research demonstrated that nutrition during the far-off (early) dry period may be as important to transition success as nutrition during the close-up dry period. Our objectives were to determine if a low-energy, high-fiber diet fed throughout the dry period improved metabolic status and production of dairy cows compared with a higher-energy diet or a 2-diet system, and to compare responses of cows and heifers to those diets. Holstein cows (n = 25 with 10 primiparous per treatment) were assigned to each of 3 diets at 60 d before expected calving. Treatment LO [40.5% wheat straw; 5.6 MJ of net energy for lactation (NEL)/kg of DM] was designed to meet but not exceed National Research Council recommendations for ad libitum intake from dry-off until calving. Treatment HI was a high-energy diet (6.7 MJ of NEL/kg of DM) fed for ad libitum intake from dry-off until calving. For the LO+HI treatment, the LO diet was fed ad libitum from dry-off until 21 d before expected calving, followed by the HI diet until parturition. After parturition all cows were fed a lactation diet (7.0 MJ of NEL/kg of DM) through 63 d postpartum. Dry matter intake and body weight were greater for HI cows prepartum, but not postpartum. When LO+HI cows were switched to the HI diet, their dry matter intake increased to match that of HI cows. Cows fed HI had greater gain of body condition before calving but lost more postpartum. Energy balance postpartum was higher for LO cows than for HI cows. Milk production, protein content, and protein yield did not differ among diets. Milk fat content and yield were highest for HI cows, lowest for LO, and intermediate for LO+HI cows. The HI cows had lower serum nonesterified fatty acids prepartum than either LO or LO+HI, but greater concentrations postpartum. Serum ß-hydroxybutyrate did not differ prepartum, but was greater for HI than for LO or LO+HI postpartum. Serum glucose and insulin were lower for LO than HI and LO+HI prepartum; insulin was lower for LO and HI than for LO+HI postpartum. The LO cows had lower liver total lipid concentration postpartum than the HI cows and LO+HI cows. Primiparous cows generally responded to diets the same as multiparous cows. The LO+HI feeding strategy provided no benefit over the LO diet. Moreover, the high-energy diet, even when fed for only 19 d before calving in the LO+HI group, resulted in increased serum ß-hydroxybutyrate and liver total lipid concentrations compared with LO.

Influence of starter protein content on growth of dairy calves in an enhanced early nutrition program.

Our objectives were to determine the effect of starter crude protein (CP) content on growth of Holstein calves from birth to 10 wk of age in an enhanced early nutrition program, and to compare the enhanced program to a conventional milk replacer program. Calves (64 female, 25 male) were assigned to 3 treatments in a randomized block design: 1) conventional milk replacer (20% CP, 20% fat) plus conventional starter [19.6% CP, dry matter (DM) basis], 2) enhanced milk replacer (28.5% CP, 15% fat) plus conventional starter, and 3) enhanced milk replacer plus high-CP starter (25.5% CP, DM basis). Calves began treatments (n=29, 31, and 29 for treatments 1 to 3) at 3 d of age. Conventional milk replacer (12.5% solids) was fed at 1.25% of birth body weight (BW) as DM daily in 2 feedings from wk 1 to 5 and at 0.625% of birth BW once daily during wk 6. Enhanced milk replacer (15% solids) was fed at 1.5% of BW as DM during wk 1 and 2% of BW as DM during wk 2 to 5, divided into 2 daily feedings. During wk 6, enhanced milk replacer was fed at 1% of BW as DM once daily. Calves were weaned at d 42. Starter was available for ad libitum intake starting on d 3. Starter intake was greater for calves fed conventional milk replacer. For calves fed enhanced milk replacer, starter intake tended to be greater for calves fed enhanced starter. During the weaning period, enhanced starter promoted greater starter DM intake than the conventional starter. Over the 10-wk study, the average daily gain of BW (0.64, 0.74, and 0.80 kg/d) was greater for calves fed enhanced milk replacer with either starter and, for calves fed enhanced milk replacer, tended to be greater for calves fed high-CP starter. Rates of change in withers height, body length, and heart girth were greater for calves fed enhanced milk replacer but did not differ between starter CP concentrations. The postweaning BW for enhanced milk replacer treatments was greater for calves receiving the enhanced starter at wk 8 (73.7, 81.3, and 85.8 kg) and wk 10 (88.0, 94.9, and 99.9 kg). Starter CP content did not affect height, length, or heart girth within enhanced milk replacer treatments. Regression analysis showed that gain of BW during the first week postweaning (wk 7) increased with greater 3-d mean starter intake in the week before weaning. Starter with 25.5% CP (DM basis) provided modest benefits in starter intake (particularly around weaning) and growth for dairy calves in an enhanced early nutrition program compared with a conventional starter (19.6% CP).

Prepartum dietary energy intake affects metabolism and health during the periparturient period in primiparous and multiparous Holstein cows.

An experiment was conducted to determine the effect of prepartum plane of energy intake on metabolic profiles related to lipid metabolism and health in blood and liver. Primiparous (n=24) and multiparous (n=23) Holsteins were randomly assigned by expected date of parturition to 1 of 3 prepartum energy intakes. A high energy diet [1.62 Mcal of net energy for lactation (NE(L))/kg; 15% crude protein] was fed for either ad libitum intake or restricted intake to supply 150% (OVR) or 80% (RES) of energy requirements for dry cows in late gestation. To limit energy intake to 100% of National Research Council requirements at ad libitum intake, chopped wheat straw was included as 31.8% of dry matter for a control diet (CON; 1.21 Mcal of NE(L)/kg of dry matter; 14.2% crude protein). Regardless of parity group, OVR cows had greater concentrations of glucose, insulin, and leptin in blood prepartum compared with either CON or RES cows; however, dietary effects did not carry over to the postpartum period. Prepartum nonesterified fatty acids (NEFA) were lower in OVR cows compared with either CON or RES cows. Postpartum, however, OVR cows had evidence of greater mobilization of triacylglycerol (TAG) from adipose tissue as NEFA were higher than in CON or RES cows, especially within the first 10 d postpartum. Prepartum ß-hydroxybutyrate (BHBA) was not affected by diet before parturition; however, within the first 10 d postpartum, OVR cows had greater BHBA than CON or RES cows. Prepartum diet did not affect liver composition prepartum; however, OVR cows had greater total lipid and TAG concentrations and lower glycogen postpartum than CON or RES cows. Frequency of ketosis and displaced abomasum was greater for OVR cows compared with CON or RES cows postpartum. Controlling or restricting prepartum energy intake yielded metabolic results that were strikingly similar both prepartum and postpartum, independent of parity group. The use of a bulky diet controlled prepartum energy intake in multiparous and primiparous cows, improved metabolic status postpartum, and reduced the incidence of health problems. When metabolic profiles are considered collectively, cows overfed energy prepartum exhibited an "overnutrition syndrome" with characteristics of clinical symptoms displayed by diabetic or obese nonruminant subjects. This syndrome likely contributed to metabolic dysfunction postpartum.

Prepartum dietary management of energy intake affects postpartum intake and lactation performance by primiparous and multiparous Holstein cows.

An experiment was conducted to determine the effect of plane of energy intake prepartum on postpartum performance. Primiparous (n=24) and multiparous (n=23) Holsteins were randomly assigned by expected date of parturition to 1 of 3 prepartum energy intakes. A moderate energy diet [1.63 Mcal of net energy for lactation (NE(L))/kg; 15% crude protein (CP)] was fed for either ad libitum intake (OVR) or restricted intake (RES) to supply 150 or 80% of National Research Council (2001) energy requirement, respectively, for dry cows in late gestation. To limit energy intake to 100% of NRC requirement at ad libitum dry matter intake (DMI), chopped wheat straw was included as 31.8% of dry matter (DM) in a control diet (CON; 1.21 Mcal of NE(L)/kg of DM; 14% CP). Multiparous and primiparous cows assigned to OVR gained body condition during the dry period [initial body condition score (BCS)=3.3], but were not overconditioned by parturition (BCS=3.5). Multiparous cows in the OVR group lost more BCS postpartum than multiparous RES or CON cows. Primiparous cows lost similar amounts of BCS among dietary treatment groups postpartum. Addition of chopped wheat straw to CON diets prevented a large decrease in DMI prepartum in both primiparous and multiparous cows. During the first 3 wk postpartum, DMI as a percentage of BW was lower for multiparous OVR cows than for multiparous RES cows. Prepartum diet effects did not carry over through the entire 8-wk lactation period. Because of greater mobilization of body stores, OVR cows had greater milk fat percentage and greater 3.5% fat-corrected milk yield during the first 3 wk postpartum. Multiparous cows assigned to OVR experienced a 55% decrease in energy balance and primiparous cows a 40% decrease in energy balance during the last 3 wk before parturition, compared with CON or RES cows that had little change. Multiparous cows fed OVR had a greater contribution of energy from body energy reserves to milk energy output than either CON or RES cows. Overfeeding energy prepartum resulted in large changes in periparturient energy balance. Even in the absence of overconditioning, a large change in DMI and energy balance prepartum influenced postpartum DMI and BCS loss, especially for multiparous cows. Chopped wheat straw was effective at controlling energy intake prepartum, although primiparous cows did not achieve predicted DMI. Even so, controlling or restricting energy intake in primiparous cows was not detrimental to lactational performance over the first 8 wk of lactation.

Productivity and hay requirements of beef cattle in a Midwestern year-round grazing system.

Our objective was to evaluate a replicated (n = 2) Midwestern year-round grazing system's hay needs and animal production compared with a replicated (n = 2) conventional (minimal land) system over 3 yr. Because extended grazing systems have decreased hay needs for the beef herd, it was hypothesized that this year-round system would decrease hay needs without penalizing animal production. In the minimal land (ML) system, two replicated 8.1-ha smooth bromegrass-orchardgrass-birdsfoot trefoil (SB-OG-BFT) pastures were rotationally stocked with six mature April-calving cows and calves and harvested as hay for winter feeding in a drylot. After weaning, calves were finished on a high-concentrate diet. Six mature April-calving cows, six mature August-calving cows, and their calves were used in the year-round (YR) grazing system. During the early and late summer, cattle grazed two replicated 8.1-ha SB-OG-BFT pastures by rotational stocking. In mid-summer and winter, April- and August-calving cows grazed two replicated 6.1-ha, endophyte-free tall fescue-red clover (TF-RC) and smooth bromegrass-red clover (SB-RC) pastures, respectively, by strip-stocking. In late autumn, spring-calving cows grazed 6.1-ha corn crop residue fields by strip-stocking. Calves were fed hay with corn gluten feed or corn grain over winter and used as stocker cattle to graze SB-OG-BFT pastures with cows until early August the following summer. First-harvest forage from the TF-RC and SB-RC pastures was harvested as hay. Body condition scores of April-calving cows did not differ between grazing systems, but were lower (P < or = 0.03) than those of August-calving cows from mid-gestation through breeding. Preweaning calf BW gains were 47 kg/ha of perennial pasture (P < 0.01) and 32 kg/cow (P = 0.01) lower in the YR grazing system than in the ML system. Total BW gains ofpreweaning calf and grazing stocker cattle were 12 kg/ha of perennial pasture less (P = 0.07), but 27 kg/cow greater (P = 0.02) in pastures in the YR grazing system than in the ML system. Amounts of hay fed to cows in the ML system were 1,701 kg DM/cow and 896 kg DM/cow-stocker pair greater (P < 0.05) than in the YR grazing system. Extended grazing systems in the Midwest that include grazing of stocker cattle to utilize excess forage growth will decrease stored feed needs, while maintaining growing animal production per cow in April- and August-calving herds.