Conceptualizing adverse childhood experiences as a latent factor: Tests of measurement invariance across five racial and ethnic groups.

Adverse childhood experiences (ACEs) are conventionally measured using a cumulative-risk index without consideration of distinct measurement properties across racial and ethnic groups. Drawing from the 2018-2020 National Survey of Children's Health (N = 93,759; 48% female; average age: 9.52 years), we assess the measurement invariance of a latent-factor ACE model across five groups: Hispanic children (14%) and non-Hispanic White (73%), Black (7%), Asian/Pacific Islander (5%), and American Indian/Alaskan Native (1%) children. Results support configural and full metric invariance across groups. However, several ACE item thresholds differed across groups. Findings highlight the potential utility of a latent factor approach and underscore the need to assess differences across racial and ethnic groups in terms of the optimal conceptualization and measurement of ACEs.

Patterns and predictors of adolescent engagement in a mindfulness-based social-emotional learning program.

INTRODUCTION: Although mindfulness-based interventions (MBIs) show promise for promoting positive youth development, little is known about student engagement in MBIs. Initial research presents mixed findings in MBI engagement related to participant characteristics, and there is a lack of research examining the influence of context on engagement, despite the critical role context plays in academic engagement. This study examines the contribution of student demographic characteristics and classroom context to MBI engagement. METHODS: Survey engagement data were collected at three time points from 106 ninth grade students (Mage = 14.17 years, 60.4% female, 44.2% Black, 24.8% Hispanic/Latino) who participated in the Be CALM program during the 2021-2022 school year. Latent growth curve modeling was used to examine trajectory of student engagement and assess student and classroom predictors of engagement. RESULTS: There was no overall change in the trajectory of student engagement, although variability was observed across classes. Identifying as Hispanic/Latino was associated with lower engagement (ß = -.25, p = .008), although this did not appear to be related to program experience. Peer connections predicted engagement at the end of the program (ß = .39, p < .001). Post hoc analyses suggested that student engagement may be related to teacher program delivery quality. CONCLUSIONS: Student engagement in MBIs appears related to classroom context more than student characteristics, although further research with larger samples is needed to assess the link between engagement and program outcomes. Findings have implications for designing school-based MBIs and training school staff to deliver them in culturally responsive ways.

Mental health profiles of depressive symptoms and personal well-being among active-duty military families.

Although some research has examined the mental health of individual family members in military families, additional research is needed that considers mental health among multiple members of the family system simultaneously and that characterizes subsets of families with distinct patterns. Mental health patterns of depressive symptoms and well-being in and among families were identified using latent profile analysis with a community sample of 236 military families with a service member (SM) parent, civilian partner, and adolescent. Drawing from the Family Adjustment and Adaptation Response model, we examined several military-related family demands (e.g., relocations, deployments) and capabilities (e.g., family cohesion, social support outside the family) as correlates of the family profiles. Three profiles emerged: thriving families (62.3% of the sample where all three family members reported relatively low depressive symptoms and high personal well-being), families with a relatively distressed SM (24.2%), and families with a relatively distressed adolescent (13.5%). Overall, there were no differences between the groups of families regarding military-related demands, yet there were differences between the groups regarding their capabilities, namely family cohesion and social support. In general, families in the thriving profile tended to have higher family cohesion and social support as reported by multiple family members compared to the other two profiles. Findings can inform the development of family needs assessments and tailored interventions (and intervention points) based on family profiles and current capabilities.

Investigating the Role of Local Board of Health Governance on Local Health Department Policymaking Behavior.

OBJECTIVES: This study sought to identify groupings of policymaking behavior among local governmental health departments (LHDs) across the country and assess whether such groupings were associated with the governance activity of their board of health (BOH). DESIGN: We conducted latent class analysis (LCA) to identify possible classes of policymaking behavior among LHDs. Once classes were identified, we used multinomial logistic regression (MLN) to estimate the association between an LHD's policymaking behavior and the governance activity of their BOH. SETTING: 2019 wave of the National Association of City and County Health Officials (NACCHO) Profile Survey. PARTICIPANTS: All LHDs with BOHs in the 2019 NACCHO Profile Survey (n = 1003). OUTCOME MEASURES: Within our MLN, our primary outcome of interest was the association between an LHD's policymaking class (the main dependent variable) and the governance activity of its BOH (the main independent variable). RESULTS: Based on our LCA, we determined our sample to be composed of what we characterized as "Limited Policy-Involvement," "Average Policy Involvement," and "Expanded Policy Involvement" LHDs. Those in the Expanded Class were more likely to be involved across all policy areas compared to the Limited and Average class, especially among social determinants of health (SDOH)-related areas. Our MLN estimated that having a BOH active in legal authority was associated with an 86% increased chance that an LHD would be in the "Average Class" compared to the "Limited Class" and having a BOH active in partnership engagement was associated with an 86% more likely chance that an LHD is in the "Expanded Class" compared to the "Average Class." CONCLUSION: Using nationally representative data on LHD activity, we found distinct groups of policymaking behavior, including a quarter of LHDs that are highly active in traditional and SDOH-related policy areas. We also found that groupings of policymaking behavior, as indicated by class designation, are strongly associated with the BOH's governance activity.

Associations between dyadic relationship quality and stepfamily functioning: A common fate modeling approach.

Clinical and empirical literatures have highlighted the value of attending to distinct dyads within stepfamilies to ensure the needs of various relationships are being met. From a family systems perspective, the growth and maintenance of positive dynamics within one dyadic relationship can yield gains in other relationships and shape the larger stepfamily environment. Research seeking to link information about dyads and larger stepfamily systems is often marked by single-informant data or measures that represent individual-level constructs. Methods intended to leverage multi-informant data as indicators of dyad- or family-level constructs (i.e., common fate modeling; CFM) offer valuable opportunities to expand our understanding of stepfamily experiences. Using a sample of 291 stepparent-parent dyads, our study uses multi-informant data and CFM to assess three dyad-level constructs (i.e., marital quality, marital confidence, and stepparent-child relationship quality) as correlates of three stepfamily-level constructs (i.e., cohesion, expressiveness, and harmony). Our findings illustrate meaningful linkages among dyadic relationships and broader stepfamily-level dynamics, specifically emphasizing the role of stepparent-child relationship quality and marital confidence in shaping stepfamily cohesion, expressiveness, and harmony. The results also signal the potential for substantive findings to vary with respect to the selected unit of analysis.

An Intervention Framework for Addressing Stigma on College Campuses: Findings From a 3-Year-Long Intervention Program.

ABSTRACT: The ongoing COVID-19 pandemic will only exacerbate the rising mental health concerns among college students. However, stigma toward such concerns continues to hinder mental health care utilization among the students, requiring urgent evidence that can help guide college campuses in implementing effective antistigma interventions. We propose and provide evidence for an intervention based on findings from a 3-year-long antistigma intervention that was implemented on a Southeastern college campus in the United States. Unique random samples of college students, totaling N = 1727 across 3 years, were recruited as participants. Each year, participants completed a preintervention and postintervention survey comprising of questions related to demographics, stigma, and mental health care knowledge. Findings indicate that the stakeholder-led intervention decreased personal stigma and increased mental health care knowledge among students who were exposed to the intervention. Further research is needed to assess feasibility and efficacy of the proposed intervention framework on other campuses.

The impact of sexual victimization on substance use disorder treatment completion: A systematic review and meta-analysis.

Background:A significant proportion of individuals seeking treatment for substance use disorders (SUDs) have experienced sexual victimization, which has been shown to disrupt the efficacy of SUD treatment services. Objective: To evaluate the relationship between lifetime sexual victimization and SUD treatment completion. Methods: Relevant literature was identified through a systematic, computerized search of nine electronic databases (May 2018) and reference harvesting, yielding 15 peer-reviewed articles published between 1992 and 2017. Two authors independently conducted title and abstract screens, full-text reviews, data abstraction, and methodological appraisals. Risk of bias was assessed using a modified mixed-methods appraisal tool. Only nine studies met criteria for the meta-analysis, which used a random-effects model. Results: Included studies yielded mixed results regarding the impact of sexual victimization on treatment completion. The meta-analysis yielded a non-significant trend of survivors of sexual victimization having slightly lower odds of completing treatment. Conclusions: Findings from this systematic review and meta-analysis did not support the impact of sexual victimization on treatment completion. Methodological limitations of the extant literature limit cross-study comparisons. Future studies should document program-related factors to improve the ability to understand relationships affecting treatment completion.

A Systematic Review of Approaches for Continuous Quality Improvement Capacity-Building.

CONTEXT: Continuous quality improvement (CQI) has become prominent in public health settings; yet, little consolidated guidance exists for building CQI capacity of community-based organizations. OBJECTIVE: To synthesize relevant literature to identify guiding principles and core components critical to building the capacity of organizations to adopt and use CQI. DESIGN: We employed a systematic review approach to assess guiding principles and core components for CQI capacity-building as outlined in the literature. ELIGIBILITY CRITERIA: Studies meeting the following criteria were eligible for review: (1) empirical, peer-reviewed journal article, evaluation study, review, or systematic review; (2) published in 2010 or later; and (3) capacity-building activities were described in enough detail to be replicable. Studies not including human subjects, published in a language other than English, or for which full text was not available were excluded. STUDY SELECTION: The initial return of records included 6557 articles, of which 1455 were duplicates. The research team single-screened titles and abstracts of 5102 studies, resulting in the exclusion of 4842 studies. Two hundred sixty-two studies were double-screened during full-text review, yielding a final sample of 61 studies from which data were extracted. MAIN OUTCOME MEASURES: Outcome measures of interest were operationalized descriptions of guiding principles and core components of the CQI capacity-building approach. RESULTS: Results yielded articles from medical education, health care, and public health settings. Findings included guiding principles and core components of CQI capacity-building identified in current practice, as well as infrastructural and contextual elements needed to build CQI capacity. CONCLUSIONS: This consolidation of guiding principles and core components for CQI capacity-building is valuable for public health and related workforces. Despite the uneven distribution of articles from health care, medical education, and public health settings, our findings can be used to guide public health organizations in building CQI capacity in a well-informed, systematic manner.

Stepparent-Child Relationships and Child Outcomes: A Systematic Review and Meta-Analysis.

An inclusive conceptualization of "family" can enable family-serving systems and professionals to leverage high-quality family relationships, wherever they are found, to support the health and well-being of individuals. Stepfamilies are an especially common family form with distinct needs and experiences, and stepparent-child relationships can take on a variety of functions with implications for family stability and individual well-being. The purpose of this systematic review and meta-analysis was to synthesize empirical associations between stepparent-child relationships and child outcomes. General findings from 56 studies highlighted significant associations between several dimensions of stepparent-child relationships and children's psychological, behavioral, social, academic, and physical well-being. Meta-analytic findings from 68 effect-size estimates further substantiated significant and positive associations between stepparent-child relationship quality and child psychological well-being (mean r = .25) and academic well-being (mean r = .23), as well as significant and negative associations between stepparent-child relationship quality and child psychological problems (mean r = -.23) and behavioral problems (mean r = -.19).

Advanced single-cell technologies to guide the development of bioengineered lungs.

Lung transplantation remains the only viable option for individuals suffering from end-stage lung failure. However, a number of current limitations exist including a continuing shortage of suitable donor lungs and immune rejection following transplantation. To address these concerns, engineering a decellularized biocompatible lung scaffold from cadavers reseeded with autologous lung cells to promote tissue regeneration is being explored. Proof-of-concept transplantation of these bioengineered lungs into animal models has been accomplished. However, these lungs were incompletely recellularized with resulting epithelial and endothelial leakage and insufficient basement membrane integrity. Failure to repopulate lung scaffolds with all of the distinct cell populations necessary for proper function remains a significant hurdle for the progression of current engineering approaches and precludes clinical translation. Advancements in 3D bioprinting, lung organoid models, and microfluidic device and bioreactor development have enhanced our knowledge of pulmonary lung development, as well as important cell-cell and cell-matrix interactions, all of which will help in the path to a bioengineered transplantable lung. However, a significant gap in knowledge of the spatiotemporal interactions between cell populations as well as relative quantities and localization within each compartment of the lung necessary for its proper growth and function remains. This review will provide an update on cells currently used for reseeding decellularized scaffolds with outcomes of recent lung engineering attempts. Focus will then be on how data obtained from advanced single-cell analyses, coupled with multiomics approaches and high-resolution 3D imaging, can guide current lung bioengineering efforts for the development of fully functional, transplantable lungs.

Patterns of Interactions Between Youth and Resident Stepmothers: A Latent Class Analysis.

Stepparent-child relationships are a core pillar of stepfamily functioning and well-being. Stepparents can take on a variety of roles in the lives of their stepchildren, ranging from de facto parents to distant acquaintances. There remain important opportunities to explore specific interactional patterns between youth and stepparents, particularly resident stepmothers. Drawing from a family systems perspective, the purpose of the current study is to explore patterns of youth-stepmother interaction across recreational, personal, academic, and disciplinary domains of family life. Latent class analysis is conducted using a representative sample of 295 youth (mean age: 15.82 years, SD = 1.63; 41% female; 65% non-Hispanic White) residing in father-stepmother households who have living nonresident mothers at Wave I of the National Longitudinal Study of Adolescent to Adult Health. The optimal latent class solution yielded four interactional patterns, labeled versatile and involved (21%), inactive (12%), casually connected (17%), and academically oriented (50%). The latent classes are also contrasted with respect to stepfamily relationship quality, youth well-being, and socio-demographic characteristics. Foremost, the results illustrate significant variability in the patterns of interactions between youth and their resident stepmothers, which could be attributed to youths' varying autonomy-seeking efforts and other complex family dynamics. Consistent with a family systems perspective, associations between youth-stepmother interactional patterns and family and youth outcomes highlight the importance of the relationship between youth and their resident stepmothers. Importantly, not all interactional patterns differed significantly across outcomes, suggesting that no one pattern is universally optimal in father-stepmother families with adolescent children.

Las relaciones entre padrastros, madrastras e hijastros son un pilar fundamental del funcionamiento y el bienestar de las familias ensambladas. Los padrastros y las madrastras pueden asumir diversos roles en las vidas de sus hijastros, desde padres de hecho a personas con una relación distante. Quedan importantes oportunidades de analizar patrones interactivos específicos entre los jóvenes y los padrastros y madrastras, particularmente las madrastras residentes. Basándonos en una perspectiva de sistemas familiares, el propósito del presente estudio es analizar patrones de interacción entre los jóvenes y las madrastras en ámbitos recreativos, personales, académicos y disciplinarios de la vida familiar. Se realiza un análisis de clases latentes utilizando una muestra representativa de 295 jóvenes (edad promedio: 15.82 años, desviación típica = 1.63; 41 % de sexo femenino; 65 % raza blanca no hispana) que viven en hogares conformados por el padre y la madrastra, y que tienen madres vivas no residentes en la fase I del "Estudio Longitudinal Nacional de Salud de Adolescentes a Adultos" (National Longitudinal Study of Adolescent to Adult Health). La solución óptima de clase latente arrojó cuatro patrones interactivos llamados adaptables e interesados (21 %), inactivos (12 %), conectados ocasionalmente (17 %), y orientados académicamente (50 %). Las clases latentes también se contrastan con respecto a la calidad de la relación de la familia ensamblada, el bienestar de los jóvenes y las características sociodemográficas. Principalmente, los resultados ilustran la variabilidad significativa en los patrones de interacciones entre los jóvenes y sus madrastras residentes, que podrían atribuirse a los distintos esfuerzos de búsqueda de autonomía por parte de los jóvenes y a otras dinámicas familiares complejas. De acuerdo con una perspectiva de sistemas familiares, las asociaciones entre los patrones interactivos entre los jóvenes y las madrastras y los resultados de las familias y los jóvenes destacan la importancia de la relación entre los jóvenes y sus madrastras residentes. Cabe señalar que no todos los patrones interactivos difirieron considerablemente entre los resultados, lo cual sugiere que ningún patrón es óptimo a nivel universal en las familias de padres y madrastras con hijos adolescentes.

Assessment of iPSC teratogenicity throughout directed differentiation toward an alveolar-like phenotype.

Considerable work has gone into creating cell therapies from induced pluripotent stem cells (iPSCs) since their discovery just over a decade ago. However, comparatively little research has been done concerning the safety of iPSCs and their progeny and specifically the mechanisms governing teratogenicity. The aim of this study was to ascertain at what developmental phase iPSCs undergoing differentiation to an alveolar-like phenotype lose their capacity to form a teratoma and uncover potential mechanisms responsible. iPSCs were differentiated using a previously published directed differentiation protocol mirroring alveolar embryogenesis. At each developmental phase cell phenotype was assessed and cells mixed with Matrigel and injected subcutaneously above the hind limbs of NSG mice to determine teratogenicity. A genetic screen of 42 genes commonly associated with teratoma formation was conducted on all the cells and any resulting teratoma. It was found that neither NKX2-1 lung progenitors nor terminally differentiated alveolar-like cells formed teratomas. As expected the expression of pluripotency markers was diminished over differentiation. However, the expression of two proteoglycans, decorin and lumican, was increased more than 3000x during differentiation. Both decorin and lumican are putative tumor suppressors with additional functions in angiogenesis, fibrosis, inflammation and autophagy. We hypothesize that the increasing expression of these proteoglycans by iPSCs as they differentiate may act to inhibit host endothelial cell recruitment when implanted resulting in the inhibition of any teratoma formation by any remaining undifferentiated iPSCs.

Stepfamily Processes and Youth Adjustment: The Role of Perceived Neighborhood Collective Efficacy.

Using a representative sample from the National Longitudinal Study of Adolescent to Adult Health and a recently developed stepfamily-process typology, this study explores three plausible functions of perceived neighborhood collective efficacy with respect to stepfamily life and youth adjustment: an ability to (1) prevent maladaptive patterns of stepfamily processes, (2) promote stepchildren's adjustment beyond the influence of stepfamily processes, and (3) protect stepchildren's adjustment when faced with maladaptive patterns of stepfamily processes. The results indicate that higher levels of perceived neighborhood collective efficacy are associated with more adaptive stepfamily processes and higher levels of youth self-esteem over time, net the influence of stepfamily processes.

Stepparents' Attachment Orientation, Parental Gatekeeping, and Stepparents' Affinity-Seeking with Stepchildren.

Remarried stepfamilies are a sizable portion of American families; in a 2011 Pew Center survey, 42% of respondents reported at least one stepfamily member. Family clinicians and researchers suggest that stepparents' ability to develop close bonds with stepchildren may be critical to the well-being of couple and family relationships. Using actor-partner interdependence models to analyze dyadic data from 291 heterosexual remarried stepfamily couples, we explored factors related to stepparents' efforts to befriend their stepchildren. Specifically, we evaluated how remarried parents' gatekeeping and stepparents' perceptions of their attachment orientations were associated with their own and their spouse's perceptions of stepparents' affinity-seeking behaviors. Securely attached stepparents and stepparents with anxious attachment orientations engaged more frequently in affinity behaviors than did stepparents with avoidant attachment orientations; there was no difference between securely attached and anxious stepparents. Stepparents' reports of parents' restrictive gatekeeping were strongly and negatively associated with both stepparents' and parents' reports of stepparent affinity-seeking (actor and partner effects). Parents' reports of their own restrictive gatekeeping were also negatively (but more weakly) associated with parents' reports of stepparent affinity-seeking. Implications for families, clinicians, and relationship researchers and theorists are discussed.

Las familias ensambladas con parejas que se vuelven a casar constituyen una parte considerable de las familias estadounidenses; en una encuesta del Pew de 2011, el 42% de los encuestados informaron tener por lo menos un familiar con familia ensamblada. Los especialistas en familia y los investigadores sugieren que la capacidad de los padrastros de desarrollar vínculos estrechos con los hijastros puede ser fundamental para el bienestar de las relaciones de pareja y familiares (Browning & Artlelt, 2012; Ganong, Coleman, Fine, & Martin, 1999). Mediante el uso de modelos de interdependencia actor-pareja para analizar los datos diádicos de 291 parejas heterosexuales de familias ensambladas con parejas que se volvieron a casar, analizamos los factores relacionados con los esfuerzos de los padrastros para hacerse amigos de sus hijastros. Específicamente, evaluamos cómo la vigilancia de los padres que volvieron a casarse y las percepciones de los padrastros de sus orientaciones de apego estuvieron asociados con sus propias percepciones y las de su cónyuge de las conductas de búsqueda de afinidad de los padrastros. Los padrastros con un vínculo de apego seguro y los padrastros con orientaciones de apego ansioso participaron con más frecuencia en conductas de afinidad que los padrastros con orientaciones de apego evasivo; no hubo diferencia entre los padrastros con vínculo de apego seguro y los de apego ansioso. Los informes de los padrastros de la vigilancia restrictiva de los padres estuvieron asociados estrechamente y negativamente con los informes de los padrastros y de los padres sobre la búsqueda de afinidad de los padrastros (efectos de la pareja y del actor). Los informes de los padres de su propia vigilancia restrictiva también estuvieron asociados negativamente (pero más levemente) con los informes de los padres sobre la búsqueda de afinidad de los padrastros. Se debaten las consecuencias para las familias, los médicos y los investigadores y teóricos de las relaciones.

Parental Involvement and Adolescents' Academic Achievement: Latent Profiles of Mother and Father Warmth as a Moderating Influence.

Parental involvement in their adolescents' education plays an important role in promoting their children's academic outcomes. Yet, more research is needed to examine the relationship between parenting practices and parental warmth as well as to consider the potential joint contribution of warmth from both fathers and mothers. Thus, the primary purpose of the current study is to examine the extent to which patterns of parental warmth across fathers and mothers moderate the association between parental involvement and adolescents' grade point average (GPA) and school engagement behaviors. Latent profile analysis was conducted to identify disparate profiles of fathers' and mothers' warmth within a nationally representative sample of 2,306 youths (51% male; mean age = 15.31 years, SD = 1.50; 77% non-Hispanic White) residing in opposite-sex, two-parent families from Wave I and II of the National Longitudinal Study of Adolescent to Adult Health. Latent-class enumeration processes support a five-profile solution characterized by differences in levels of parental warmth and congruency across parents: (a) Congruent High Warmth, (b) Congruent Moderate Warmth, (c) Congruent Low Warmth, (d) Incongruent High Mother/Low Father Warmth, and (e) Incongruent Low Father/Lower Mother Warmth. Subsequent multiple linear regression analyses reveal a moderating effect for Congruent Low Warmth on the relationship between parental involvement and adolescents' GPA. Ultimately, the results show that variation in parental warmth exists across fathers and mothers with differing impact on adolescents' outcomes. Excluding one parent without considering the joint effects of both parents will not produce an accurate and precise understanding of parenting in research or practice.

La participación de los padres en la educación de sus hijos adolescentes desempeña un papel importante en el fomento de los resultados académicos de sus hijos. Sin embargo, se necesitan más investigaciones para analizar la relación entre las prácticas de crianza y la calidez de los padres y para tener en cuenta el posible aporte conjunto de calidez tanto de los padres como de las madres. Por lo tanto, el propósito principal del presente estudio es analizar el grado hasta el cual los patrones de calidez parental de los padres y las madres moderan la asociación entre la participación de los padres y el promedio de calificaciones de los adolescentes y las conductas de participación escolar. Se realizaron análisis de clases latentes para reconocer los diversos perfiles de calidez de los padres y las madres dentro de una muestra representativa a nivel nacional de 2306 jóvenes (51% masculina; edad promedio = 15.31 años, Desviación Típica= 1.50; 77% blanca no hispana) que vivían en familias formadas por dos padres del sexo opuesto de las fases I y II del Estudio Longitudinal Nacional de Salud del Adolescente al Adulto. Los procesos de enumeración de clases latentes respaldan una solución de cinco perfiles caracterizados por diferencias en los niveles de calidez parental y congruencia entre los padres: (a) calidez congruente alta, (b) calidez congruente moderada, (c) calidez congruente baja, (d) calidez incongruente, alta en la madre/baja en el padre, y (e) calidez incongruente, baja en el padre/más baja en la madre. Los análisis posteriores de regresión lineal múltiple revelan un efecto moderador para la calidez congruente baja en la relación entre la participación parental y el promedio de calificaciones de los adolescentes. Finalmente, los resultados demuestran que existe una variación en la calidez parental entre padres y madres con efectos muy distintos en los resultados de los adolescentes. Excluir a un padre sin tener en cuenta los efectos conjuntos de ambos padres no generará una comprensión certera y precisa de la crianza en la investigación o la práctica.

Hostile interactions in the family: Patterns and links to youth externalizing problems.

In line with family systems theory, we examined patterns of hostile interactions within families and their associations with externalizing problems among early-adolescent children. Using hostility scores based on observational data of six dyadic interactions during a triadic interaction (n = 462) (i.e., child-to-mother, mother-to-child, child-to-father, father-to-child, mother-to-father, father-to-mother)-Latent Profile Analysis supported three distinct profiles of hostility. The Low/Moderate Hostile profile included families with the lowest levels of hostility across dyads; families in the Mutual Parent-Child Hostile profile scored higher on parent-child hostility, but lower on interparental hostility; the Hostile Parent profile showed higher levels of parent-to-child and interparental hostility, but lower child-to-parent hostility. Concerning links to youth outcomes, youth in the Mutual Parent-Child Hostile profile reported the highest level of externalizing problems, both concurrently and longitudinally. These results point to the importance of examining larger family patterns of hostility to fully understand the association between family hostility and youth adjustment.

A Typology of Interactional Patterns Between Youth and Their Stepfathers: Associations with Family Relationship Quality and Youth Well-Being.

Stepfamilies are an increasingly common family form, many of which are headed by a resident mother and stepfather. Stepfather-child relationships exert notable influence on stepfamily stability and individual well-being. Although various stepfather roles have been observed, more research is warranted by which stepfather-child interactions are explored holistically and across a variety of life domains (e.g., recreational, personal, academic, and disciplinary). Thus, the primary purpose of the current study is to explore varying interactional patterns between youth and their stepfathers. A latent class analysis is conducted using a representative sample of 1,183 youth (53% female; mean age = 15.64 years, SD = 1.70 years; 62% non-Hispanic White) residing in mother-stepfather families from Wave I of the National Longitudinal Study of Adolescent to Adult Health. Latent-class enumeration processes support a four-class solution, with latent classes representing inactive, academically oriented, casually connected, and versatile and involved patterns of youth-stepparent interaction. Notable differences and similarities are evident across patterns with respect to family relationship quality, youth well-being, and socio-demographic characteristics. Differences are most stark between the inactive and versatile and involved patterns. Ultimately, the results showcase notable variation in youth-stepparent interactional patterns, and one size does not necessarily fit all stepfamilies. Family practitioners should be mindful of variation in youth-stepparent interactional patterns and assist stepfamilies in seeking out stepparent-child dynamics that are most compatible with the needs and dynamics of the larger family system.

Las familias ensambladas son cada vez más una forma común de familia, muchas de las cuales están encabezadas por una madre y un padrastro residentes. Las relaciones entre el padrastro y los niños ejercen una influencia notable en la estabilidad de la familia ensamblada y en el bienestar individual. Aunque se han observado distintos roles de los padrastros, se necesitan más investigaciones en las que las interacciones entre el padrastro y los niños se analicen íntegramente y en diferentes ámbitos de la vida (p. ej.: recreativo, personal, académico y disciplinario). Por lo tanto, el objetivo principal del presente estudio es analizar patrones interactivos variados entre los jóvenes y sus padrastros. Se realiza un análisis de clases latentes utilizando una muestra representativa de 1183 jóvenes (el 53 % de sexo femenino; edad promedio = 15.64 años, DT = 1.70 años; el 62 % blancos no hispanos) que viven en familias compuestas por una madre y un padrastro de la serie I del "Estudio Longitudinal Nacional de la Salud Adolescente a Adulta" (National Longitudinal Study of Adolescent to Adult Health). Los procesos de enumeración de las clases latentes respaldan una solución de cuatro clases, donde las clases latentes representan patrones de interacción inactivos, orientados a lo académico, conectados ocasionalmente y versátiles e interesados entre los jóvenes y los padrastros. Se observan diferencias y similitudes notables entre los patrones con respecto a la calidad de la relación familiar, al bienestar de los jóvenes y a las características sociodemográficas. Las diferencias son más marcadas entre los patrones inactivos y versátiles e interesados. Finalmente, los resultados exhiben una variación notable en los patrones interactivos entre los jóvenes y los padrastros, por lo tanto, no necesariamente existe un criterio único aplicable a todas las familias esambladas. Los médicos familiares deben estar al tanto de la variación en los patrones interactivos entre los jóvenes y los padrastros y ayudar a las familias ensambladas a buscar una dinámica entre los jóvenes y los padrastros que sea más compatible con las necesidades y la dinámica del sistema familiar más amplio.

Expanding and characterizing esophageal epithelial cells obtained from children with eosinophilic esophagitis.

BACKGROUND: The role of epithelial cells in eosinophilic esophagitis (EoE) is not well understood. In this study, our aim was to isolate, culture, and expand esophageal epithelial cells obtained from patients with or without EoE and characterize differences observed over time in culture. METHODS: Biopsies were obtained at the time of endoscopy from children with EoE or suspected to have EoE. We established patient-derived esophageal epithelial cell (PDEEC) lines utilizing conditional reprogramming methods. We determined integrin profiles, gene expression, MHC class II expression, and reactivity to antigen stimulation. RESULTS: The PDEECs were found to maintain their phenotype over several passages. There were differences in integrin profiles and gene expression levels in EoE-Active compared to normal controls and EoE-Remission patients. Once stimulated with antigens, PDEECs express MHC class II molecules on their surface, and when co-cultured with autologous T-cells, there is increased IL-6 and TNF-α secretion in EoE-Active patients vs. controls. CONCLUSION: We are able to isolate, culture, and expand esophageal epithelial cells from pediatric patients with and without EoE. Once stimulated with antigens, these cells express MHC class II molecules and behave as non-professional antigen-presenting cells. This method will help us in developing an ex vivo, individualized, patient-specific model for diagnostic testing for causative antigens.

Production of high purity alveolar-like cells from iPSCs through depletion of uncommitted cells after AFE induction.

Protocols to differentiate induced pluripotent stem cells (iPSCs) into specialized cells are continually evolving. iPSCs can be differentiated to alveolar cells with protocols that focus on development, specifically by inducing differentiation into definitive endoderm (DE), anterior foregut endoderm (AFE) and then lung bud progenitor intermediaries. However, current protocols result in a relatively low yield of the desired alveolar cells. The aim of this study was to evaluate whether depleting uncommitted cells after AFE induction would have a beneficial effect on alveolar cell yield. iPSCs were differentiated on Matrigel-coated plates for 25days. At each stage, phenotype was assessed using flow cytometry, immunofluorescence and qRT-PCR. Additionally, samples were dissociated in trypsin following AFE induction to improve the purity of the cells for the subsequent lung differentiation phase. Finally, the efficacy of dissociating the samples was confirmed comparing the expression of markers indicative of pluripotency and apoptosis. The ability to differentiate iPSCs to DE was 96% and to AFE was 97% utilizing our current protocol. After depletion of uncommitted cells and 12 days in culture, the purity of lung bud progenitors was 99%. Finally, the percentage of alveolar types I and II at the end of differentiation was 74% as compared to 31% in control cultures that had not been depleted of uncommitted cells after AFE induction. In conclusion, depletion of uncommitted cells after AFE induction, improves terminal differentiation of alveolar cells from 31% to 74%.

Bidirectional Relations Between Parenting and Behavior Problems From Age 8 to 13 in Nine Countries.

This study used data from 12 cultural groups in nine countries (China, Colombia, Italy, Jordan, Kenya, Philippines, Sweden, Thailand, and the United States; N = 1,298) to understand the cross-cultural generalizability of how parental warmth and control are bidirectionally related to externalizing and internalizing behaviors from childhood to early adolescence. Mothers, fathers, and children completed measures when children were ages 8-13. Multiple-group autoregressive, cross-lagged structural equation models revealed that child effects rather than parent effects may better characterize how warmth and control are related to child externalizing and internalizing behaviors over time, and that parent effects may be more characteristic of relations between parental warmth and control and child externalizing and internalizing behavior during childhood than early adolescence.