ABSTRACT
Two pairs of unprecedented ß-carboline-phenylpropanoid heterogeneous alkaloids, (±)-pheharmines A-B (1-4), characterized by a morpholino[4,3,2-hi]ß-carboline core with two chiral centers, were isolated from the roots of Peganum harmala. The structures, including their absolute configurations, were identified using spectroscopic analyses and electronic circular dichroism (ECD) calculations. The biosynthetic hypothesis for the formation of pheharmines A-B was proposed. Compounds 1-4 exhibited moderate cytotoxic activities against HL-60 cell lines.
Subject(s)
Alkaloids , Peganum , Humans , Peganum/chemistry , Peganum/metabolism , Morpholinos/analysis , Morpholinos/metabolism , Seeds , Molecular Structure , Alkaloids/pharmacology , Alkaloids/chemistry , Carbolines/pharmacology , Carbolines/chemistryABSTRACT
OBJECTIVES: We aimed to compare the efficiency of prostate cancer (PCa) detection using a radiomics signature based on advanced zoomed diffusion-weighted imaging and conventional full-field-of-view DWI. METHODS: A total of 136 patients, including 73 patients with PCa and 63 without PCa, underwent multi-parametric magnetic resonance imaging (mp-MRI). Radiomic features were extracted from prostate lesion areas segmented on full-field-of-view DWI with b-value = 1500 s/mm2 (f-DWIb1500), advanced zoomed DWI images with b-value = 1500 s/mm2 (z-DWIb1500), calculated zoomed DWI with b-value = 2000 s/mm2 (z-calDWIb2000), and apparent diffusion coefficient (ADC) maps derived from both sequences (f-ADC and z-ADC). Single-imaging modality radiomics signature, mp-MRI radiomics signature, and a mixed model based on mp-MRI and clinically independent risk factors were built to predict PCa probability. The diagnostic efficacy and the potential net benefits of each model were evaluated. RESULTS: Both z-DWIb1500 and z-calDWIb2000 had significantly better predictive performance than f-DWIb1500 (z-DWIb1500 vs. f-DWIb1500: p = 0.048; z-calDWIb2000 vs. f-DWIb1500: p = 0.014). z-ADC had a slightly higher area under the curve (AUC) value compared with f-ADC value but was not significantly different (p = 0.127). For predicting the presence of PCa, the AUCs of clinical independent risk factors model, mp-MRI model, and mixed model were 0.81, 0.93, and 0.94 in training sets, and 0.74, 0.92, and 0.93 in validation sets, respectively. CONCLUSION: Radiomics signatures based on the z-DWI technology had better diagnostic accuracy for PCa than that based on the f-DWI technology. The mixed model was better at diagnosing PCa and guiding clinical interventions for patients with suspected PCa compared with mp-MRI signatures and clinically independent risk factors. KEY POINTS: ⢠Advanced zoomed DWI technology can improve the diagnostic accuracy of radiomics signatures for PCa. ⢠Radiomics signatures based on z-calDWIb2000 have the best diagnostic performance among individual imaging modalities. ⢠Compared with the independent clinical risk factors and the mp-MRI model, the mixed model has the best diagnostic efficiency.
Subject(s)
Multiparametric Magnetic Resonance Imaging , Prostatic Neoplasms , Diffusion Magnetic Resonance Imaging , Humans , Magnetic Resonance Imaging , Male , Prostatic Neoplasms/diagnostic imagingABSTRACT
BACKGROUND: Despite the efficacy of absolute ethanol (EtOH), its radiolucency introduces several risks in interventional therapy for treating vascular malformations. This study aims to develop a novel radiopaque ethanol injection (REI) to address this issue. METHODS: Iopromide is mixed with ethanol to achieve radiopacity and improve the physicochemical properties of the solution. Overall, 82 male New Zealand white rabbits are selected for in vivo radiopacity testing, peripheral vein sclerosis [animals were divided into the following 5 groups (n = 6): negative control (NC, saline, 0.250 ml/kg), positive control (EtOH, 0.250 ml/kg), low-dose REI (L-D REI, 0.125 ml/kg), moderate-dose REI (M-D REI, 0.250 ml/kg), and high-dose REI (H-D REI 0.375 ml/kg)], pharmacokinetic analyses (the blood sample was harvested before injection, 5 min, 10 min, 20 min, 40 min, 1 h, 2 h, 4 h, and 8 h after injection in peripheral vein sclerosis experiment), peripheral artery embolization [animals were divided into the following 5 groups (n = 3): NC (saline, 0.250 ml/kg), positive control (EtOH, 0.250 ml/kg), L-D REI (0.125 ml/kg), M-D REI (0.250 ml/kg), and H-D REI (0.375 ml/kg)], kidney transcatheter arterial embolization [animals were divided into the following 4 groups (n = 3): positive control (EtOH, 0.250 ml/kg), L-D REI (0.125 ml/kg), M-D REI (0.250 ml/kg), and H-D REI (0.375 ml/kg); each healthy kidney was injected with saline as negative control], and biosafety evaluations [animals were divided into the following 5 groups (n = 3): NC (0.250 ml/kg), high-dose EtOH (0.375 ml/kg), L-D REI (0.125 ml/kg), M-D REI (0.250 ml/kg), and H-D REI (0.375 ml/kg)]. Then, a prospective cohort study involving 6 patients with peripheral venous malformations (VMs) is performed to explore the clinical safety and effectiveness of REI. From Jun 1, 2023 to August 31, 2023, 6 patients [age: (33.3 ± 17.2) years] with lingual VMs received sclerotherapy of REI and 2-month follow-up. Adverse events and serious adverse events were evaluated, whereas the efficacy of REI was determined by both the traceability of the REI under DSA throughout the entire injection and the therapeutic effect 2 months after a single injection. RESULTS: The REI contains 81.4% ethanol (v/v) and 111.3 mg/ml iodine, which can be traced throughout the injection in the animals and patients. The REI also exerts a similar effect as EtOH on peripheral venous sclerosis, peripheral arterial embolization, and renal embolization. Furthermore, the REI can be metabolized at a similar rate compared to EtOH and Ultravist® and did not cause injury to the animals' heart, liver, spleen, lungs, kidneys and brain. No REI-related adverse effects have occurred during sclerotherapy of VMs, and 4/6 patients (66.7%) have achieved complete response at follow-up. CONCLUSION: In conclusion, REI is safe, exerts therapeutic effects, and compensates for the radiolucency of EtOH in treating VMs. TRIAL REGISTRATION: The clinical trial was registered as No. ChiCTR2300071751 on May 24 2023.
Subject(s)
Ethanol , Vascular Malformations , Animals , Rabbits , Ethanol/therapeutic use , Ethanol/pharmacology , Male , Vascular Malformations/therapy , Vascular Malformations/drug therapy , Humans , Contrast Media/pharmacokinetics , Contrast Media/pharmacology , Contrast Media/therapeutic use , Iohexol/analogs & derivativesABSTRACT
OBJECTIVE: To evaluate the feasibility of S2 alar iliac screw insertion in Chinese children using computerized three-dimension reconstruction and simulated screw placement technique, and to optimize the measurement of screw parameters. METHODS: A total of 83 pelvic CT data of children who underwent pelvic CT scan December 2018 to December 2020 were retrospectively analyzed, excluding fractures, deformities, and tumors. There were 44 boys and 39 girls, with an average age of (10.66±3.52) years, and were divided into 4 groups based on age (group A:5 to 7 years old;group B:8 to 10 years old;group C:11-13 years old;group D:14 to 16 years old). The original CT data obtained were imported into Mimics software, and the bony structure of the pelvis was reconstructed, and the maximum and minimum cranial angles of the screws were simulated in the three-dimensional view with the placement of 6.5 mm diameter S2 alar iliac screws. Subsequently, the coronal angle, sagittal angle, transverse angle, total length of the screw, length of the screw in the sacrum, width of the iliac, and distance of the entry point from the skin were measured in 3-Matic software at the maximum and minimum head tilt angles, respectively. The differences among the screw parameters of S2 alar iliac screws in children of different ages and the differences between gender and side were compared and analyzed. RESULTS: In all 83 children, 6.5 mm diameter S2 iliac screws could be placed. There was no significant difference between the side of each screw placement parameter. The 5 to 7 years old children had a significantly smaller screw coronal angle than other age groups, but in the screw sagittal angle, the difference was more mixed. The 5 to 7 years old children could obtain a larger angle at the maximum head tilt angle of the screw, but at the minimum cranial angle, the larger angle was obtained in the age group of 11 to 13 years old. There were no significant differences among the age groups. The coronal angle and sagittal angle under maximum cephalic angle and minimum cranial angle of 5 to 7 years old male were (40.91±2.91)° and (51.85±3.75)° respectively, which were significantly greater than in female. The coronal angle under minimum cranial angle was significantly greater in girls aged 8-10 years old than in boys. For the remaining screw placement angle parameters, there were no significant differences between gender. The differences in the minimum iliac width, the screw length, and the length of the sacral screws showed an increasing trend with age in all age groups. The distance from the screw entry point to the skin in boys were significantly smaller than that of girls. The minimum width of the iliac in boys at 14 to 16 years of age were significantly wider than that in girls at the same stage. In contrast, in girls aged 5 to 7 years and 11 to 13 years, the screw length was significantly longer than that of boys at the same stage. CONCLUSION: The pelvis of children aged 5 to 16 years can safely accommodate the placement of 6.5 mm diameter S2 alar iliac screws, but the bony structures of the pelvis are developing and growing in children, precise assessment is needed to plan a reasonable screw trajectory and select the appropriate screw length.
Subject(s)
Ilium , Spinal Fusion , Humans , Male , Female , Child , Adolescent , Child, Preschool , Ilium/surgery , Retrospective Studies , Feasibility Studies , Bone Screws , Pelvis , Sacrum/surgery , Spinal Fusion/methodsABSTRACT
The dysfunction of islet ß-cells is one of the causes of diabetes, and lncRNA Gm10451 is also a participant in the occurrence and the development of various diseases. This study was carried out to reveal the correlation within ß-cells and Gm10451. Our study was started with the cellular cultivation of MIN6 cells in vitro, where this islet ß-cell line was randomly divided into the groups of control, hyperglycemia, Gm10451 siRNA tansfection, and Gm10451 tansfection. Of all these treatments, cells in the groups of Gm10451 siRNA tansfection and Gm10451 tansfection were given with lentiviral transfection under hyperglycemia condition. Further explorations were established using PCR assay and MTT method to evaluate Gm10451 expression and estimate cellular proliferation. It ended up with the enzyme-linked immunosorbent assay (ELISA) to assess Caspase 3 activity, superoxide dismutase (SOD) activity, and reactive oxygen species (ROS) content and the secretion of IL-10 and IL-1. It was found that Gm10451 expression in MIN6 cells under hyperglycemia cultivation was notably higher than the control group; likewise, a transfection with the lentivirus of Gm10451 also resulted in the upregulation of Gm10451 expression, succeeded with inhibiting cellular proliferation, enhancing Caspase 3 activity, and decreasing SOD activity. In the lentivirus transfection groups, transfection of Gm10451 elevated the ROS content and promoted IL-1 expression, and it also decreased both IL-10 expression and insulin secretion, leading to a consequence of statistically significant difference in contrast to the high-glucose group; on the contrary, transfection of Gm10451 siRNA in a high-glucose environment downregulated the expression of Gm10451 and inversed those change before, whose results were statistically significant when compared with the high-glucose group. Hyperglycemia promotes the expression of Gm10451. Targeting inhibition toward Gm10451 alleviates cellular apoptosis and the oxidative stress of islet cells, promoting proliferation and insulin secretion of islet cells.
ABSTRACT
The chemical profile and phytotoxic activity of the essential oil extracted from Artemisia sieversiana was investigated. In total 17 compounds were identified by GC/MS, representing 99.17% of the entire oil, among which α-thujone (64.46%) and eucalyptol (10.15%) were the most abundant constituents. The major components, their mixture as well as the essential oil exhibited significant phytotoxic activity against Amaranthus retroflexus, Medicago sativa, Poa annua and Pennisetum alopecuroides, with their IC50 values ranged from 1.55 â¼ 6.21 mg/mL (α-thujone), 1.42 â¼ 17.81 mg/mL (eucalyptol), 0.23 â¼ 1.05 mg/mL (the mixture), and 1.89 â¼ 4.69 mg/mL (the essential oil) on the four tested species. The mixture of the major constituents exerted more potent effect compared with each individual compound, indicating the possible involvement of synergistic effect of these two compounds.
Subject(s)
Amaranthus , Artemisia , Oils, Volatile , Poa , Artemisia/chemistry , Eucalyptol/pharmacology , Oils, Volatile/chemistry , Oils, Volatile/pharmacologyABSTRACT
Eight cephalotaxine-type alkaloids (1-8), including two new compounds cephafortunines A and B (1-2), were isolated from the branches and leaves of Cephalotaxus fortunei var. alpina. Their structures were identified by a series of spectroscopic methods (MS, UV, IR, 1D, and 2D NMR) and comparison with the reported data of known analogs. The absolute configurations of 1 and 2 were determined by electronic circular dichroism (ECD) calculations. 1-8 were evaluated for their in vitro antiproliferation effects against two human leukemia cell lines (U937 and HL-60). All compounds showed different levels of antiproliferation effects against U937 cells with GI50 values of 4.21-23.70 µM. 4 and 5 were the most active against U937 cells with GI50 values of 4.21 and 6.58 µM and against HL-60 cells with GI50 values of 6.66 and 6.70 µM, respectively. 4 and 5 arrested HL-60 cell cycle in G0/G1 phase.
Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Cephalotaxus/chemistry , Harringtonines/pharmacology , Antineoplastic Agents, Phytogenic/isolation & purification , China , HL-60 Cells , Harringtonines/isolation & purification , Humans , Molecular Structure , Phytochemicals/isolation & purification , Phytochemicals/pharmacology , Plant Leaves/chemistry , U937 CellsABSTRACT
BACKGROUND: Non-small cell lung cancer (NSCLC) is the most common cancer worldwide. Circular RNAs (circRNAs) are recently identified as important gene regulators with critical roles in cancer biology. In this study, we focus on the effect of circ_0000376 targeting miR-384 on malignant phenotypes of NSCLC cells. METHODS: Circ_0000376 and miR-384 expression in NSCLC tissue samples were measured using qRT-PCR. The association between pathological parameters and the circ_0000376 expression was analyzed as well. Human NSCLC cell lines A549 and NCI-H460 were used as cell models. CCK-8 and BrdU assay were used to assess the effect of circ_0000376 on NSCLC cell line proliferation and drug sensitivity. Transwell assay was conducted to detect the effect of circ_0000376 on migration and invasion. Further, luciferase reporter assay was employed to validate the targeting of miR-384 by circ_0000376. RESULTS: Circ_0000376 expression in NSCLC clinical samples was up-regulated and this was linked to unfavorable pathological parameters. Circ_0000376 markedly accelerated the proliferation and metastasis, and enhanced chemoresistance of NSCLC cells. Mechanically, circ_0000376 overexpression could bind with miR-384 and repress its expression. CONCLUSIONS: Circ_0000376 is a newly discovered oncogenic circRNA in NSCLC, and can be potentially regarded as a diagnostic biomarker and therapy target.
Subject(s)
Carcinoma, Non-Small-Cell Lung/genetics , Lung Neoplasms/genetics , MicroRNAs/biosynthesis , RNA, Long Noncoding/biosynthesis , A549 Cells , Carcinoma, Non-Small-Cell Lung/drug therapy , Carcinoma, Non-Small-Cell Lung/pathology , Cell Proliferation/physiology , Drug Resistance, Neoplasm , Humans , Lung Neoplasms/drug therapy , Lung Neoplasms/pathology , MicroRNAs/genetics , Middle Aged , Neoplasm Metastasis , RNA, Long Noncoding/geneticsABSTRACT
Knowledge gap exists to understand the soil CO2 emission and microbial group response to substrates of whole plant residues and derived biochar. We used 13C-labelled substrates (rice straw, roots and biochar) to track influences of their decomposition on soil priming effect (PE) and phospholipid fatty acid (PLFA) composition during one-year incubation. Organic substrates at 1% (w/w) level increased soil pH, available nitrogen (AN) and available phosphorus (AP), especially during the first 45 days of incubation. After incubation, 44% of the added straw was mineralized to 13CO2, followed by roots (~35%) and biochar (~5%). Straw and roots amendment caused positive PE during 4-360 day of the incubation, where a lowest value of 41.9 mg C kg-1 was observed. Biochar amendment caused negative PE during 56-150 day of the incubation, where a largest value of -99.0 mg C kg-1 was observed. Analysis of 13C-labelled PLFA enabled the differentiation of microbial groups during substrates utilization. Gram positive bacteria (G+) and general bacteria groups were dominated in co-metabolizing both the native soil organic carbon (SOC) and substrates after straw and roots amendment. Gram negative bacteria (G-), especially identified by PLFA biomarkers cy17:0 and cy19:0, preferentially utilizes the 13C-labelled biochar but not promoting soil priming effect. Soil pH, SOC, AN and AP all explained changes of total and 13C-labelled PLFA contents (>75%, p < .05). Evidences showed that biochar is best in sequestering soil C pool, followed by straw and roots, and soil microbial groups in utilization of organic substances mediated SOC mineralization.
Subject(s)
Microbiota , Oryza , Carbon , Charcoal , Soil , Soil MicrobiologyABSTRACT
Baicalensines A (1) and B (2) were isolated from the roots of Thalictrum baicalense and structurally characterized using spectroscopic data, 13C NMR calculations, and the CASE algorithm. Compound 1, representing a new class of alkaloid dimers, contains berberine conjugated to a ring-opened isoquinoline. Compound 2 is the first reported natural benzylisoquinoline bearing a formyl group at C-3. Plausible biosynthetic pathways are proposed. Compound 1 exerted moderate cytotoxicity against the Caco-2 and HL-60 cell lines.
Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Berberine/pharmacology , Thalictrum/chemistry , Alkaloids/chemistry , Alkaloids/isolation & purification , Alkaloids/pharmacology , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Berberine/chemistry , Berberine/isolation & purification , Caco-2 Cells , HL-60 Cells , Humans , Isoquinolines/chemistry , Isoquinolines/isolation & purification , Isoquinolines/pharmacology , Molecular Structure , Plant Roots/chemistryABSTRACT
A heavy metal-resistant bacterial strain was isolated from heavy metal-contaminated soils and identified as Burkholderia sp. J62 based on the 16S rDNA gene sequence analysis. The heavy metal- and antibiotic resistance, heavy metal solubilization of the isolate were investigated. The isolate was also evaluated for promoting plant growth and Pb and Cd uptakes of the plants from heavy metal-contaminated soils in pot experiments. The isolate was found to exhibit different multiple heavy metal and antibiotic resistance characteristics. Atomic absorption spectrometer analysis showed increased bacterial solubilization of lead and cadmium in solution culture and in soils. The isolate produced indole acetic acid, siderophore and 1-aminocyclopropane-1-carboxylate deaminase. The isolate also solubilized inorganic phosphate. Inoculation with the isolate was found to significantly (p<0.05) increase the biomass of maize and tomato plants. Increase in tissue Pb and Cd contents varied from 38% to 192% and from 5% to 191% in inoculated plants growing in heavy metal-contaminated soils compared to the uninoculated control, respectively. These results show that heavy metal-solubilizing and plant growth promoting bacteria are important for plant growth and heavy metal uptake which may provide a new microbial enhanced-phytoremediation of metal-polluted soils.
Subject(s)
Burkholderia/isolation & purification , Metals, Heavy/metabolism , Metals, Heavy/toxicity , Plant Development , Biodegradation, Environmental , Biomass , Burkholderia/drug effects , Burkholderia/genetics , Cadmium/metabolism , Cadmium/toxicity , Drug Resistance, Bacterial , Lead/metabolism , Lead/toxicity , Solanum lycopersicum/growth & development , Solanum lycopersicum/metabolism , Molecular Sequence Data , Plants/metabolism , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Soil/analysis , Soil Microbiology , Soil Pollutants/metabolism , Soil Pollutants/toxicity , Zea mays/growth & development , Zea mays/metabolismABSTRACT
Low anterior resection (LAR) with total mesorectal excision has been considered a standard treatment for patients with rectal cancer. However, the functional outcome and life quality of laparoscopic LAR (LLAR) in Chinese patients remain unclear. A cohort of 51 Chinese patients (22 men and 29 women) who had undergone LLAR was included in this study. Anorectal manometry combined with the Wexner scores questionnaire were applied to assess functional outcome preoperatively (1 week) and postoperatively (at 3, 6 and 9 months). The validated Chinese versions of the European Organization for Research and Treatment of Cancer QLQ-C30 and QLQ-CR38 questionnaires were also used to assess the patients' quality of life at the indicated time points. The results demonstrated that the manometric parameters exhibited a temporary decrease at 3 months postoperatively, but a gradual increase at 6 and 9 months, while the Wexner scores exhibited an opposite trend. Furthermore, patients with high anastomoses had significantly higher manometric parameters, a lower frequency of incontinence and lower Wexner scores compared with those with low anastomoses at 9 months (all P<0.05). For the entire cohort, quality of life at 3 months postoperatively was worse compared with the preoperative level, but returned to normal by 9 months. Patients with high anastomoses exhibited significantly better role, emotional and social function, had a better body image and sexual function, fewer problems with defecation and lower frequency of diarrhea, as well as fewer chemotherapy-related side effects at 6 months postoperatively when compared with the low anastomosis group (all P<0.05). In conclusion, LLAR is generally acceptable for Chinese patients with rectal cancer, particularly for those with middle or high rectal cancer, in terms of functional outcome and quality of life.
ABSTRACT
OBJECTIVE: To explore clinical efficacy of thoracolumbar fractures fixation with pedicle screws fixation at the level of the fracture and monosegment bone graft simultaneously. METHODS: Retrospective analysis of 32 patients with thoracolumbar fractures underwent surgical procedure of pedicle screws fixation at the level of the fracture and monosegment bone graft simultaneously from January 2006 to December 2008. All the patients were followed up more than two years. There were 25 males and 7 females with an average age of 39.1 years (ranged, 25 to 60 years). According to the AO classification, type A1.3 was in 5 cases, type A3.1 in 17, type A3.3 in 8, type C1.1 in 1, and type C1.3 in 1. Load scoring was from 4 to 7 points with average of 5.8 points. The spinal cord function according to Frankel grade, grade A was in 2 cases, grade B in 2, grade C in 5, grade D in 9 and grade E in 14. Cobb angle, the height of anterior border of vertebral body, spinal canal stenosis rate were observed by X-ray films. Meanwhile, pain and work ability were evaluated by Denis scale. RESULTS: All the patients were followed-up from 30 to 48 months (averaged, 39.2 months). No internal fixation loosening or breakage were found. Three cases occurred with floating callus and other obtained well bone fusion. Compared with preoperation, Cobb angle, the height of anterior border of vertebral body, and spinal canal stenosis rate improved obviously (P < 0.05); but Cobb angle had lost partially at the last follow-up (P > 0.05). Meanwhile, anterosuperior part of vertebral body of 25 cases existed cavity phenomenon. The nerve function of all cases recovered for 1-2 grades, except for 2 cases without change whose spinal cord function was degree A preoperatively. According to Denis scale, lower back pain scoring, P1 was in 22 cases, P2 in 7, P3 in 3; state of work scoring, W1 was in 18 cases, W2 in 8, W3 in 3, W5 in 3. CONCLUSION: It can help to correct the kyphosis and improve low back pain in thoracolumbar fractures through pedicle screws fixation at the level of the fracture and monosegment bone graft simultaneously, but it can not improve the cavity phenomenon of injured vertebral body and avoid partially lost of Cobb angle.
Subject(s)
Lumbar Vertebrae/surgery , Spinal Fractures/surgery , Thoracic Vertebrae/surgery , Adult , Bone Screws , Female , Follow-Up Studies , Fracture Fixation, Internal/instrumentation , Humans , Male , Middle Aged , Retrospective StudiesABSTRACT
The plant growth promotion characteristics of a heavy-metal-resistant strain of Bacillus edaphicus NBT was characterized. The strain was also evaluated for promoting plant growth and lead (Pb) uptake of Brassica juncea L. Czern (Indian mustard) in soil artificially contaminated with 0, 400, and 800 mg Pb.kg-1 soil. Atomic absorption spectrometer analysis demonstrated that strain NBT could release water-soluble Pb from lead carbonate in the solution. Strain NBT had the capacity to produce indole acetic acid, siderophores, and 1-aminocyclopropane-1-carboxylate deaminase. Low and high Pb treatments significantly decreased the growth of Indian mustard. Inoculation with strain NBT was found to increase root dry mass (ranging from 16% to 22%) and above-ground tissue dry mass (ranging from 24% to 30%) of Indian mustard in the Pb-amended soil. Strain NBT was able to mobilize Pb efficiently in plants in Pb-amended soil. In the soil treated with 400 and 800 mg Pb.kg-1 soil, the increase in Pb uptake varied from 18% to 46% in live bacterium-inoculated Indian mustard plants compared with dead bacterium-inoculated control. The strain was also able to colonize and develop in the rhizosphere soil of Indian mustard after root inoculation.
Subject(s)
Bacillus/metabolism , Lead/metabolism , Mustard Plant/growth & development , Soil Pollutants/metabolism , Biodegradation, Environmental , Biomass , Hydrogen-Ion Concentration , Lead/analysis , Mustard Plant/metabolism , Mustard Plant/microbiology , Plant Roots/metabolism , Plant Roots/microbiology , Soil Pollutants/analysisABSTRACT
Two lead (Pb)-resistant endophytic bacteria were isolated from rape roots grown in heavy metal-contaminated soils and characterized. A pot experiment was conducted for investigating the capability of the two isolates to promote the growth and Pb uptake of rape from Pb-amended soil. The two isolates were identified as Pseudomonas fluorescens G10 and Microbacterium sp. G16 based on the 16S rDNA gene sequence analysis. Strains G10 and G16 exhibited different multiple heavy metal and antibiotic resistance characteristics and increased water-soluble Pb in solution and in Pb-added soil. Root elongation assays demonstrated increases in root elongation of inoculated rape seedlings compared to the control plants. Strain G16 produced indole acetic acid, siderophores and 1-aminocyclopropane-1-carboxylate deaminase. Increases in biomass production and total Pb uptake in the bacteria-inoculated plants were obtained compared to the control. The two strains could colonize the root interior and rhizosphere soil of rape after root inoculation.