[Rapid preliminary diagnosis of anaerobic bacterial infections. I. Natural fluorescence of pus, purulent fluids and dressing under UV radiation]. / Szybka, wstepna diagnostyka zakazen bakteriami beztlenowymi. I. Fluorescencja naturalna ropy, plynów ropnych i opatrunków pod wplywem promieniowania UV.

For evaluation of usefulness of natural fluorescence of clinical materials in UV radiation as rapid diagnostic method of infections with anaerobes, 405 samples of pus, bloody-purulent fluids, blood, wound secretions, dressings and other materials were investigated. Occurrence of red-brick UV fluorescence of clinical materials was compared with results of culture aimed at isolation of non-sporeforming anaerobes from "B. melaninogenicus group (P. melaninogenica, P. intermedia and P. saccharolytics). Significant correlation red-brick fluorescence of clinical materials resulting from UV irradiation with presence in these materials of anaerobes such as P. melaninogenica, P. intermedia and P. asaccharolytics was detected. Investigation of clinical materials with application of fluorescence in UV radiation lasts only 1-2 minutes and together with preparation and microscopical inspection which is Gram-stained--only 15-20 min. Positive results of this test may constitute a basis for rapid, preliminary identification of the etiologic factor and for direction of chemotherapy in the early period of infection.

[Rapid preliminary diagnosis of anaerobic bacterial infections. II. Investigation of the presence of volatile and non-volatile fatty acids (C1-C6) in pus, purulent fluids and dressing by application of gas chromatography]. / Szybka wstepna diagnostyka zakazen bakteriami beztlenowymi. II. Badanie obecnosci lotnych i nielotnych kwasów tluszczowych (C1-C6) w ropie, plynach ropnych i opatrunkach przy zastosowaniu chromatografii gazowej.

Studies were performed in the years 1986-1990 and dealing with 405 clinical materials originating from infections within the abdominal cavity, maxilla, reproductive organ, post-operative wounds as well as dressings, extremities, blood, bile and other types of material and usefulness of analysis by gas chromatography of presence of lower fatty acids in materials for rapid and preliminary diagnostics with strictly anaerobic bacteria was confirmed. Presence in chromatograms of isobutyric, butyric isovaleric, valeric, isocaproic and caproic acid, and partly of propionic acid, is a good indication of infection with strictly anaerobic bacteria. Moreover, presence of succinic acid may suggest presence of anaerobic infection, similarly as presence in chromatograms of three or more various lower fatty acids. Rapid, approximative diagnosis of anaerobic bacteria by application of gas chromatography is worth of broader application. Investigation of clinical material by this method lasts only 1-2 hours and obtained results my present a basis for preliminary diagnosis of the etiological factor and for direction of chemotherapy, which is very important in most early period of treatment of infections.

[Study of obligate anaerobic bacterial sensitivity to tinidazole and metronidazole (determination of minimal inhibiting concentration--MIC)]. / Badania wrazliwosci bakterii bezwzglednie beztlenowych na tynidazol i metronidazol (oznaczanie najmniejszych hamujacych stezen--MIC).

Comparative susceptibility testing of 428 strains of obligate anaerobic bacteria belonging to genera Propionibacterium, Arachnia, Actinomyces, Bacteroides, Prevotella, Porphyromonas, Anaerorhabdus, Fibrobacter, Fusobacterium, Peptostreptococcus and Clostridium to metronidazole and tinidazole was performed. The study of the susceptibility of anaerobic bacteria was carried out by the method of serial dilution in Brucella agar according to Finegold and Sutter (1972). Strains of B. fragilis species, B. fragilis group, other Bacteroides spp.. Fusobacterium spp. and Clostridium spp. were susceptible to both chemotherapeutics in clinically attainable concentrations. Of particular significance is the greater susceptibility of these bacteria to tinidazole. Taking into account this activity, tinidazole is a superior agent in the treatment of anaerobic infections. Both chemotherapeutics were not active against, rarely isolated from severe infections. Gram-positive anaerobic rods from genera Propionibacterium. Actinomyces and Arachnia and were partially active against peptostreptococci.

[Hydrogen peroxide, chloramine T and chlorhexidrine in the disinfection of acrylic resin]. / Woda utleniona, chloramina T i dwuglukonian chlorheksydyny jako srodki do odkazania tworzywa akrylowego.

The effectiveness of 3% h drogen peroxide, 5% chloramine T and 0,5% chlorhexidine gluconate solutions in disinfection of acrylic resine plates massively infected with oral flora was analysed. The acrylic resine plates used for investigations, were infected in vitro with mixed salivary flora characterized by small numbers of yeast-like fungi (1st group), or great number of these microorganisms (2nd group). Infected plates were exposed to solutions of analysed disinfectants during various time periods. After rinsing or inactivation of disinfectant residues, acrylic plates were put into bacteriological medium and incubated during 7 days period in 37 degrees C. The results of this study indicated the effectiveness of acrylic plates disinfection to be dependent on used disinfectant, time of exposition, and microorganisms present on the surface of acrylic resine. The solutions of disinfectants were less active in the cases of plates infected with material containing great numbers of yeast-like microorganisms. Among analysed disinfectants 0,5% solution of chlorhexidine was characterized by most effective and rapid activity, whereas 3% solution of hydrogen peroxide was found to be the least effective.

Beneficial effects of cardiac rehabilitation in patients with incomplete revascularization after primary coronary angioplasty.

BACKGROUND: There are no reliable data concerning the safety and benefits of physical rehabilitation in patients with a two-vessel disease before the second stage of angioplasty. The aim of this study was to evaluate the efficiency of early cardiac rehabilitation in patients with acute coronary syndromes and with angiographically significant residual coronary artery stenosis after a successful percutaneous coronary intervention (PCI) into the culprit lesion. DESIGN: Retrospective analysis of the results of coronary angiograms and exercise tests of patients who underwent stationary rehabilitation after their first ACS and first PCI. SETTING: Cardiac Rehabilitation Department. POPULATION: One hundred ninety patients divided into 2 groups according to the completeness of myocardial revascularization; 49 with significant (≥70%) coronary artery stenosis in a non-culprit vessel, the mean diameter reduction 80±9%; and 141 without any residual stenosis. The prevalence of classical risk factors was comparable in both groups. Rehabilitation was conducted as a stationary 3-week program. METHODS: Comparison of the initial and final exercise test workload in both groups, as well as the frequency of adverse effects during the program. RESULTS: Physical training in patients with incomplete revascularization (IR) was safe and well tolerated. Significant increase of workload capacity after the rehabilitation program was observed in both groups: in the IR group from 7.3±3.0 to 8.8±2.9 MET (P<0.0001) and in the complete revascularization (CR) group - from 7.6±2.8 to 9.2±2.9 MET (P<0.0001). No significant difference was observed in initial workload capacities (P=0.9813) nor in final workload capacities (P=0.8571) between the two groups. Two patients in the group with residual lesion (4%) and one in the group without residual lesion (0.7%) required urgent PCI during the rehabilitation program, P=0.1637. CONCLUSION: Early postinfarction physical training is safe and efficient for patients after complete revascularization and for those with untreated non-culprit coronary artery stenosis. Gradual increase in physical training intensity under cardiologist supervision is essential in identifying those rare patients for whom the second stage of angioplasty should not be delayed. CLINICAL REHABILITATION IMPACT: Our study shows that patients with incomplete revascularization may be qualified for cardiac rehabilitation programs.

[Sterinole and cetylpirydine chloride influence on non-sporulated anaerobic bacteria of oral cavity]. / Dzialanie sterinolu i chlorku cetylopirydyniowego na nieprzetrwalnikujace bakterie beztlenowe jamy ustnej.

Sensibility (MBC--Minimal Bactericidal Concentration) of 97 strains of non-sporulated anaerobic bacteria separated from clinical material from oral cavity to Sterinole (Polfa) and cetylpirydine chloride has been examined. Experiments have been carried out by means of suspension method adapted appropriately to the tests on non-sporulated anaerobes. 72 hours' cultures of strains containing 10(9) of live bacterial cells in 1 ml have been used as inoculum. MBC readings have been performed after 7 days' incubation at temperature of (37 degrees C) 310 degrees K. From the total number of 97 strains under examinations 34 strains were sensible to low concentration of Sterinole equal to 7.8 to 15.5 micrograms/ml. Further 19 strains exacted MBC within 15.6 to 31.1 micrograms/ml and further 40 strains perished in the antiseptic concentration of 31.2 to 62.4 micrograms/ml. The remaining 4 strains exacted concentration of 62.5 to 125 micrograms/ml to be perished. Among 97 strains 29 strains were perishing in low concentrations of cetylpirydine chloride equal to 7.8 to 15.5 micrograms/ml. Successive 25 strains were damaging by the antiseptic concentration equal to 15.6 to 31.1 micrograms/ml. Further 28 strains exacted utilization of concentration equal to 31.2 to 62.4 micrograms/ml while in case of the remaining 15 strains MBC values achieved the level of 62.5 to 125 micrograms/ml. Strains from Leptotrichia buccalis and then Gram--positive anaerobic bacteria were the most sensitive ones both to Sterinole and cetylpirydine chloride while Gram--negative anaerobic bacteria and anaerobic micrococcus were less sensitive.