ABSTRACT
Cadmium was administered in drinking water at a concentration of 50 ppm to female Wistar rats for 5 months before mating, and then continued during gestation. The histochemical evaluation revealed a relative decrease in succinic dehydrogenase (SDH) and Mg+2-stimulated ATPase activities in duodenum, liver and kidney in the exposed rats. It is suggested that the resulting decrease in metabolic efficiency in maternal organs would be expected to exert an indirect, inhibitive effect on maternal-fetal transfer of nutrients, as reflected by fetal growth retardation.
Subject(s)
Cadmium/toxicity , Duodenum/enzymology , Kidney/enzymology , Liver/enzymology , Adenosine Triphosphatases/metabolism , Administration, Oral , Animals , Ca(2+) Mg(2+)-ATPase , Copper/metabolism , Duodenum/drug effects , Female , Iron/metabolism , Kidney/drug effects , Kidney/ultrastructure , Liver/drug effects , Maternal-Fetal Exchange , Pregnancy , Rats , Rats, Inbred Strains , Succinate Dehydrogenase/metabolism , Zinc/metabolismABSTRACT
1. Long trem benzene action brings about a permanent decrease in oxidoreductive enzymes and active transport as well as an inactivation of the lysosomal apparatus in liver-cells. 2. Particular liver lobule zones show different sensitivity to toxic action of benzene which might result from their different role in the processes of benzene biotransformation.
Subject(s)
Acid Phosphatase/metabolism , Benzene/poisoning , Calcium-Transporting ATPases/metabolism , Liver/enzymology , NADH Tetrazolium Reductase/metabolism , NADH, NADPH Oxidoreductases/metabolism , Succinate Dehydrogenase/metabolism , Animals , Chronic Disease , Liver/pathology , Male , MiceABSTRACT
The investigations were performed on mice. They were divided into a control group and 4 experimental groups. The experimental animals were administered intraperitoneally benzene 6 X every 24 h. The animals were decapitated 30 min. 4, 12 and 24 h after the last benzene administration. During the experiment, dyeing for neutral lipids and glycogen was carried out, and the activity of NADH2-r.t., SDH, G-6-Pase, ATP-ase and ACP was estimated. A decrease of glycogen content in liver cells, deviations in the amount of neutral lipids, reversible decrease of mitochondrial enzymes activity, and intensification of the processes of intracellular catabolism were found.
Subject(s)
Benzene/poisoning , Liver/drug effects , Acid Phosphatase/metabolism , Adenosine Triphosphatases/metabolism , Animals , Benzene/metabolism , Biotransformation , Glucose-6-Phosphatase/metabolism , Lipid Metabolism , Liver/enzymology , Liver/metabolism , Liver Glycogen/metabolism , Mice , NADH Tetrazolium Reductase/metabolism , Succinate Dehydrogenase/metabolismABSTRACT
The experiment was carried out on rats, which were divided into three experimental and one control groups. The experimental animals were intraperitoneally injected with furfural in the dose of 58 mg/kg body weight for 30 days. In the liver samples obtained at autopsy, apart from routine staining with hematoxylin and eosin, estimation of the activity of the following enzymes was made: succinic dehydrogenase. NADH-tetrazol reductase, lactic dehydrogenase, glucose-6-phosphate, adenosine-triphosphatase, Ca-formol, glucose-6-phosphatase and acid phosphatase. Glycogen content was also evaluated. A temporary decrease in the activity of reactions for the enzymes of tissue respiration, an increase in the activity of glucose-6-phosphatase with a simultaneous decrease of glycogen content, activation of intracellular digestive processes, and inhibition of active transport through biological membranes were found in animals intoxicated with furfural.