Pulsed Electromagnetic Field (PEMF) Treatment Ameliorates Murine Model of Collagen-Induced Arthritis.

Rheumatoid arthritis (RA) is an autoimmune disease of the joint synovial membranes. RA is difficult to prevent or treat; however, blocking proinflammatory cytokines is a general therapeutic strategy. Pulsed electromagnetic field (PEMF) is reported to alleviate RA's inflammatory response and is being studied as a non-invasive physical therapy. In this current study, PEMF decreased paw inflammation in a collagen-induced arthritis (CIA) murine model. PEMF treatment at 10 Hz was more effective in ameliorating arthritis than at 75 Hz. In the PEMF-treated CIA group, the gross inflammation score and cartilage destruction were lower than in the untreated CIA group. The CIA group treated with PEMF also showed lower serum levels of IL-1ß but not IL-6, IL-17, or TNF-α. Serum levels of total anti-type II collagen IgG and IgG subclasses (IgG1, IgG2a, and IgG2b) remained unchanged. In contrast, tissue protein levels of IL-1ß, IL-6, TNF-α, receptor activator of nuclear factor kappa-Β (RANK), RANK ligand (RANKL), IL-6 receptor (IL-6R), and TNF-α receptor1 (TNFR1) were all lower in the ankle joints of the PEMF-treated CIA group compared with the CIA group. The results of this study suggest that PEMF treatment can preserve joint morphology cartilage and delay the occurrence of CIA. PEMF has potential as an effective adjuvant therapy that can suppress the progression of RA.

Promotion of Colitis in B Cell-Deficient C57BL/6 Mice Infected with Enterotoxigenic Bacteroides fragilis.

Enterotoxigenic Bacteroides fragilis (ETBF) causes colitis and is implicated in inflammatory bowel diseases and colorectal cancer. The ETBF-secreted B. fragilis toxin (BFT) causes cleavage of the adherence junction, the E-cadherin, resulting in the large intestine showing IL-17A inflammation in wild-type (WT) mice. However, intestinal pathology by ETBF infection is not fully understood in B-cell-deficient mice. In this study, ETBF-mediated inflammation was characterized in B-cell-deficient mice (muMT). WT or muMT C57BL/6J mice were orally inoculated with ETBF and examined for intestinal inflammation. The indirect indicators for colitis (loss of body weight and cecum weight, as well as mortality) were increased in muMT mice compared to WT mice. Histopathology and inflammatory genes (Nos2, Il-1ß, Tnf-α, and Cxcl1) were elevated and persisted in the large intestine of muMT mice compared with WT mice during chronic ETBF infection. However, intestinal IL-17A expression was comparable between WT and muMT mice during infection. Consistently, flow cytometry analysis applied to the mesenteric lymph nodes showed a similar Th17 immune response in both WT and muMT mice. Despite elevated ETBF colonization, the ETBF-infected muMT mice showed no histopathology or inflammation in the small intestine. In conclusion, B cells play a protective role in ETBF-induced colitis, and IL-17A inflammation is not attributed to prompted colitis in B-cell-deficient mice. Our data support the fact that B cells are required to ameliorate ETBF infection-induced colitis in the host.

DNA-binding Cell-penetrating Peptide-based TRAIL Over-expression in Adipose Tissue-derived Mesenchymal Stem Cells Inhibits Glioma U251MG Growth.

BACKGROUND/AIM: Genetic manipulation of stem cells using non-viral vectors is still limited due to low transfection efficiency. We investigated whether the DNA-binding cell-permeation peptides (CPP) can enhance the transfection efficiency of non-viral vectors in adipose tissue-derived mesenchymal stem cells (ASCs) and whether ASCs over-expressing TRAIL through CPP can inhibit the growth of glioma U251MG cells in vitro and in vivo. MATERIALS AND METHODS: ASCs were genetically engineered to over-express TRAIL by using CPP, pCMV3-TRAIL and lipid-based transfection reagents (X-tremeGENE). RESULTS: The transfection efficiency of ASCs increased by approximately 7% using CPP; 53.9% of ASCs were transfected and TRAIL expression in ASCs increased by approximately 3 times compared to X-tremeGENE alone. ASCs over-expressing TRAIL using CPP inhibited growth of glioma U251MG cells both in vitro and in the U251MG xenograft model. CONCLUSION: CPP can be used as an enhancer for genetically manipulating ASCs and tumor treatment.

TRAIL-overexpressing Adipose Tissue-derived Mesenchymal Stem Cells Efficiently Inhibit Tumor Growth in an H460 Xenograft Model.

BACKGROUND/AIM: Mesenchymal stem cell-based tumor therapy is still limited due to the insufficient secretion of effectors and discrepancies between their in vitro and in vivo efficacy. We investigated whether genetically engineered adipose tissue-derived mesenchymal stem cells (ASCs) overexpressing tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had inhibitory effects on H460 tumor growth both in vitro and in an H460 xenograft model. MATERIALS AND METHODS: Genetically engineered adipose tissue-derived mesenchymal stem cells (ASCs) overexpressing tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) were obtained from plasmid transfection with pCMV3-TRAIL and -interferon (IFN)-ß (producing ASC-TRAIL and ASC-IFN-ß, respectively). Death of H460 cells co-cultured with ASCs, ASC-TRAIL, and ASC-IFN-ß or exposed to their conditioned medium was evaluated via apoptosis and cytotoxicity assays. In addition, in an H460 xenograft model (n=10 per group), the antitumor potential of TRAIL-overexpressing, and IFN-ß-overexpressing ASCs was investigated. RESULTS: Conditioned medium obtained from ASC-IFN-ß increased apoptosis of H460 cells more than did ASC-TRAIL. Additionally, in H460 xenograft models, while native ASCs promoted tumor growth, ASC-TRAIL and ASC-IFN-ß both dramatically suppressed tumor growth. Interestingly, in the context of ASC-IFN-ß, tumors were detected only in 20% of nude mice, with smaller sizes and lower weights than those of the control group. CONCLUSION: TRAIL-overexpressing ASCs can be used to treat tumors; ASC-IFN-ß in particular secrete a higher level of TRAIL.

Numerical investigation on the flow characteristics and aerodynamic force of the upper airway of patient with obstructive sleep apnea using computational fluid dynamics.

Developing a mathematical model to predict the abnormal flow characteristics that are produced by obstructive sleep apnea is an important step in learning the pathophysiology of the obstructive sleep apnea (OSA) disease. The present study provides detailed calculations of flow in the pharyngeal airway of a patient with obstructive sleep apnea. To achieve this goal, a computational fluid dynamics model was constructed using raw data from three-dimensional computed tomogram (CT) images of an OSA patient. To reproduce the important transition from laminar to turbulent flow in the pharyngeal airway, the low Reynolds number k-epsilon model was adopted and successfully validated using previous open literature. The results show that the flow in the pharyngeal airway of patients with OSA comprises a turbulent jet formed by area restriction at the velopharynx. This turbulent jet causes higher shear and pressure forces in the vicinity of the velopharynx. From the results, It may be deduced that the most collapsible area in the pharyngeal airway of OSA patients is the velopharynx where minimum intraluminal pressure and maximum aerodynamic force lie.

A novel multi-stage direct contact membrane distillation module: Design, experimental and theoretical approaches.

An economic desalination system with a small scale and footprint for remote areas, which have a limited and inadequate water supply, insufficient water treatment and low infrastructure, is strongly demanded in the desalination markets. Here, a direct contact membrane distillation (DCMD) process has the simplest configuration and potentially the highest permeate flux among all of the possible MD processes. This process can also be easily instituted in a multi-stage manner for enhanced compactness, productivity, versatility and cost-effectiveness. In this study, an innovative, multi-stage, DCMD module under countercurrent-flow configuration is first designed and then investigate both theoretically and experimentally to identify its feasibility and operability for desalination application. Model predictions and measured data for mean permeate flux are compared and shown to be in good agreement. The effect of the number of module stages on the mean permeate flux, performance ratio and daily water production of the MDCMD system has been theoretically identified at inlet feed and permeate flow rates of 1.5 l/min and inlet feed and permeate temperatures of 70 °C and 25 °C, respectively. The daily water production of a three-stage DCMD module with a membrane area of 0.01 m2 at each stage is found to be 21.5 kg.

In situ electrokinetic remediation of As-, Cu-, and Pb-contaminated paddy soil using hexagonal electrode configuration: a full scale study.

We investigated the in situ applicability of the electrokinetic process with a hexagonal electrode configuration in order to remediate arsenic (As)-, copper (Cu)-, and lead (Pb)-contaminated paddy rice field soil at a field scale (width 17 m, length 12.2 m, and depth 1.6 m). An iron electrode was used in order to prevent the severe acidification of the soil near the anode. We selected ethylenediaminetetraacetic acid (EDTA) as a pursing electrolyte to enhance the extraction of Cu and Pb. The system removed 44.4% of the As, 40.3% of the Cu, and 46.6% of the Pb after 24 weeks of operation. Fractionation analysis showed that the As bound to amorphous ion (Fe) and aluminum (Al) oxyhydroxides was changed into a form of As specifically bound. In the case of Cu and Pb, the fraction bound to Fe-Mn oxyhydroxide primarily decreased. The EDTA formed negatively charged complexes with Cu and Pb, and those complexes were transported toward the anode. The energy consumption was very low compared to that on a small scale because there was less energy consumption due to Joule heating. These results show that the in situ electrokinetic process could be applied in order to remediate paddy rice fields contaminated with multiple metals.

Dynamic free-surface deformations in axisymmetric liquid bridges.

Thermocapillary convection in a differentially heated liquid bridge is investigated in a two-dimensional numerical study. The deformable free surface is obtained as a solution of the coupled transport equations at fixed unit Prandtl number and aspect ratio. Only steady convection can be realized in this axisymmetric computation with either non-deformable or deformable surfaces. Dynamic free-surface deformations do not induce transitions to oscillatory convection even at large Reynolds numbers. Free surfaces are convex near the cold wall due to large surface pressures at the stagnation-point. Near the hot wall they change from concave to convex with increasing Re due to large normal viscous stresses. Large Capillary numbers cause additional surface ripples there while having little effect on the interior. Heat loss from the free surface has dramatic influence on the dynamics and must be included in theoretical models of thermocapillary instabilities.

Field application of electrokinetic remediation for multi-metal contaminated paddy soil using two-dimensional electrode configuration.

In this study, we evaluated the feasibility of in situ electrokinetic remediation for arsenic (As)-, copper (Cu)-, and lead (Pb)-contaminated soil, in a pilot-scale field application with two-dimensional electrode configurations. Square and hexagonal configurations with different electrode spacing, 1 m and 2 m, were investigated under a constant 100 V. A square configuration with electrode spacing of 2 m removed 61.5 % of As, 11.4 % of Cu, and 0.9 % of Pb, respectively, and a hexagonal configuration with the same spacing showed a higher removal efficiency in top (59 % of As, 0-0.5 m) and middle (53 % of As, 0.5-1.0 m) layers, but much lower removal efficiency in the bottom layer (1-1.5 m), which was thought to be due to groundwater flow through periodic rise and fall of tides. Fractionation analysis showed that As bound to Fe-Mn oxyhydroxide was the main form of As removed by the electrokinetic process. The two-dimensional configuration wasted less electrical energy by Joule heating, and required fewer electrode installations, compared to the one-dimensional electrode configuration.