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Anal Sci ; 32(3): 301-5, 2016.
Article in English | MEDLINE | ID: mdl-26960609

ABSTRACT

Metal nanoparticles express unique light-scattering characteristics based on the localized surface plasmon resonance, which depends on the metal species, particle size, and aggregation state of the nanoparticles. Therefore, we focused on the light-scattering characteristics of metal nanoparticles, such as silver, gold, and copper oxide, adsorbed on a bacterium. Monodisperse silver nanoparticles expressed the strongest scattered light among them, and showed various colors of scattered light. Although a monodisperse gold nanoparticle produced monochromatic light (green color), the color of the scattered light strongly depended on the aggregation state of the nanoparticles on a bacterium. On the other hand, copper oxide nanoparticles expressed monochromatic light (blue color), regardless of their aggregation states on a bacterium. We examined details concerning the light-scattering characteristics of metal nanoparticles, and discussed the possibility of their applications to bacterial cell imaging.


Subject(s)
Biosensing Techniques/methods , Metal Nanoparticles/chemistry , Pseudomonas aeruginosa/chemistry , Scattering, Radiation , Single-Cell Analysis/methods , Adsorption , Biosensing Techniques/instrumentation , Copper/chemistry , Gold/chemistry , Light , Microscopy, Electron, Scanning , Pseudomonas aeruginosa/cytology , Silver/chemistry , Single-Cell Analysis/instrumentation , Surface Properties
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