Establishment and characterization of a HER2-enriched canine mammary cancerous myoepithelial cell line.

BACKGROUND: Canine mammary tumors (CMTs) have a poor prognosis, along with tumor recurrence and metastasis. Cell lines are vital in vitro models for CMT research. Many CMT epithelial cell lines were reported. However, canine mammary myoepithelial cells, the contractile component of the canine mammary tissue were overlooked. This study aimed at establishing such a cell line. CMT-1 cell line was obtained from a canine mammary tumor CMT-1 and characterized molecularly through qPCR, western blotting, immunochemistry and immunofluorescence. Its doubling time, cytogenetic analysis and migration rate were evaluated using growth study, karyotype analysis and wound healing assay respectively. To determine its tumorigenesis, xenograft transplantation was performed. RESULTS: CMT-1 tumor was a complex canine mammary carcinoma that stained negative to estrogen receptors (ER) and progesterone receptors (PR), but positive to human epidermal growth receptor-2 (HER2), defined as HER2-enriched subtype. In this study, a CMT-1 cell line obtained from CMT-1 tumor was immune-positive to vimentin, α-SMA, p63 and negative to E-cadherin (E-cad), indicating CMT-1 cells were myoepithelial cells. It was successfully cultured for more than 50 passages showing the same immunoreactivity to ER, PR, and HER2 as the primary canine tumor. The doubling time of CMT-1 cell line was 26.67 h. The chromosome number of CMT-1 cells ranged from 31 to 64. A potential spontaneous epithelial to mesenchymal transition (EMT) was noticed during cell cultures. Potential EMT-induced CMT-1 cells showed no significance in migration rate compared to the original CMT-1 cells. CMT-1 cells was able to grow on a 3D culture and formed grape-like, solid, and cystic mammospheres at different time period. Inoculation of CMT-1 cells induced a complex HER2-enriched mammary tumor with metastasis in mice. CONCLUSIONS: A canine cancerous HER2-enriched myoepithelial cell line was successfully established and a canine mammosphere developed from myoepithelial cells was documented in this study. We are expecting this novel cell line and its associated mammospheres could be used as a model to elucidate the role of myoepithelial cells in CMT carcinogensis in the future.

Interfacially Super-Assembled Benzimidazole Derivative-Based Mesoporous Silica Nanoprobe for Sensitive Copper (II) Detection and Biosensing in Living Cells.

Mesoporous silica nanoparticles (MSNs) functionalized with benzimidazole-derived fluorescent molecules (DHBM) are fabricated via a feasible interfacial superassembly strategy for the highly sensitive and selective detection of Cu2+ . DHBM-MSN exhibits an obvious quenching effect on Cu2+ in aqueous solutions, and the detection limit can be as low as 7.69×10-8  M. The DHBM-MSN solid-state sensor has good recyclability, and the silica framework can simultaneously improve the photostability of DHBM. Two mesoporous silica nanoparticles with different morphologies were specially designed to verify that nanocarriers with different morphologies do not affect the specific detectionability. The detection mechanism of the fluorescent probe was systematically elucidated by combining experimental results and density function theory calculations. Moreover, the detection system was successfully applied to detect Cu2+ in bovine serum, juice, and live cells. These results indicate that the DHBM-MSN fluorescent sensor holds great potential in practical and biomedical applications.

An attack-defense game model in infrastructure networks under link hiding.

Our increasing dependence on infrastructure networks leads to growing concerns over the protection of these networks. Many methods have been proposed to select protective strategies by combining complex network theory and game theory. However, the misleading effect of hidden links is not considered in previous methods. This work creates an information gap between attackers and defenders by partly hiding network links to mislead the attacker in the game. We first introduce the rule of link hiding that depends on the nodes' property, where the number of hidden links has a maximum value. Additionally, based on the Stackelberg game model, we establish an attack-defense game model with link hiding strategies considering node property and cost constraints. Finally, we conduct experiments in a scale-free network and an existing power grid. The experimental results show that the defender tends to combine first-mover advantage and link hiding to get a better payoff under more different costs of the nodes. Hiding half of the links in the existing power grid can effectively reduce network damage by about 22.8% on average, with the two sides investing the same resources. The effect of link hiding could be more obvious when the attacker owns more resources than the defender. When an attacker employs the high-degree attacking strategy, the proposed link hiding method can help the defender reduce the damage to the network by 12.2% compared to the link reconnecting method.

Attack-Defense Game Model with Multi-Type Attackers Considering Information Dilemma.

Today, people rely heavily on infrastructure networks. Attacks on infrastructure networks can lead to significant property damage and production stagnation. The game theory provides a suitable theoretical framework for solving the problem of infrastructure protection. Existing models consider only the beneficial effects that the defender obtains from information gaps. If the attacker's countermeasures are ignored, the defender will become passive. Herein, we consider that a proficient attacker with a probability in the game can fill information gaps in the network. First, we introduce the link-hiding rule and the information dilemma. Second, based on the Bayesian static game model, we establish an attack-defense game model with multiple types of attackers. In the game model, we consider resource-consistent and different types of distributions of the attacker. Then, we introduce the solution method of our model by combining the Harsanyi transformation and the bi-matrix game. Finally, we conduct experiments using a scale-free network. The result shows that the defender can be benefited by hiding some links when facing a normal attacker or by estimating the distribution of the attacker correctly. The defender will experience a loss if it ignores the proficient attacker or misestimates the distribution.

Electrospinning Superassembled Mesoporous AIEgen-Organosilica Frameworks Featuring Diversified Forms and Superstability for Wearable and Washable Solid-State Fluorescence Smart Sensors.

Flexible optical sensors are widely studied and applied in many fields. However, developing highly stable and washable wearable sensors in optics is still facing significant challenges. Here, we demonstrate an AIEgen-organosilica framework (TPEPMO) hybrid nanostructure-based flexible optical sensor, which is prepared by a two-step co-condensation and electrospinning superassembly process. Organosilica precursors with aggregation-induced emission (AIE) features are covalently linked into periodic mesoporous organosilica (PMO) frameworks with high fluorescent efficiency due to the restriction of intramolecular motion. The three-dimensional space of ordered porous materials provides abundant reaction sites, allowing rapid and sensitive monitoring of analytes. TPEPMOs exhibit good properties as acidic pH fluorescent sensors with a pKa of 4.3. A flexible film is obtained by dispersing TPEPMO nanospheres in a poly(lactic-co-glycolic acid) (PLGA) and polyacrylonitrile (PAN) hybrid fibrous matrix (TPEPMO-CFs) using the electrospinning superassembly technique and is successfully served as an efficient fluorescent probe for the naked eye detection of ammonia gas and HCl vapor by emission changes. The fluorescence of TPEPMO-CFs can be reversed in the presence of volatile acidic/alkaline gas for more than five cycles, exhibiting excellent recyclability. In addition, TPEPMO-CF sensors show excellent washability and long-term photostability (fluorescence was maintained above 94% after washing 10 times). These stimuli-responsive AIEgen-organosilica frameworks featuring diversified forms and superstability for wearable and washable solid-state fluorescence exhibit great potential for smart gas sensors, wearable devices, and solid-state lighting applications.

High thermoelectric performance of Ag doped SnTe polycrystalline bulks via the synergistic manipulation of electrical and thermal transport.

Ernzerhof (HSE) hybrid functional approximations based on the density functional theory (DFT) method were applied to investigate the electronic band structures of SnTe. First principles calculations indicate that Ag substitution in SnTe could effectively modify the valence band structures and decrease the energy separation between valence bands, ΔEVBL-Σ, which will enhance the Seebeck coefficient. All the fabricated Ag doped SnTe samples show the same crystal structure as cubic SnTe. Compared to the pure SnTe sample, the Ag doped ones exhibit greatly enhanced thermoelectric performance, especially at high temperatures, with the highest figure-of-merit of around 1.35 achieved at 900 K, by concurrent optimization of the electrical and thermal transport properties.

Electron localization in niobium doped CaMnO3 due to the energy difference of electronic states of Mn and Nb.

The electron localization in Nb-doped CaMnO3 is analyzed in terms of the space and energy distribution of electronic states employing first-principles calculations. The energy difference of Mn 3d states and Nb 4d states makes NbO6 octahedra impede electrical conduction, so the random distribution of Nb in lattices leads to the localization of electrons near the bottom of the conduction bands. Therefore, although more carriers are introduced when Nb-doping content increases, both the electrical conductivity and absolute thermopower decrease in Nb heavy doped CaMnO3. The calculated transport properties agree well with the experimental data, supporting the analysis of localization.

[Comparative field study on high flow rate samplers for respirable fraction-A solution to smaller collected masses].

OBJECTIVE: Dust sample mass gain is too smaller to satisfy the limit of detection (LOD) even in most cases during dust sampling at workplaces nowdays, especially for respirable fraction. Therefore, it is aimed to solve the problem by increasing sample load with high flow rate samplers. METHODS: In A and B two shipyards respirable welding fume was sampled by high flow rate cyclone samplers of FSP-10 (10 L/min) for 2-2.5 hours and normal flow rate FSP-2 (2 L/min) for 3-4 hours with a stratigy of parallele sampling at the same workpalce, in order to compare their mass gain, coincidence rate with LOD, and airborn dust concentration. RESULTS: Sample mass gain of 0.97±0.40 mg and 1.61±0.86 mg respectively in the two factories by FSP-10 was significantly higher than that of 0.29±0.12 mg and 0.51±0.27 mg by FSP-2 (t-test, P<0.05 in both cases) , increasing herewith the coincidence rate with LOD from 26.8% (when sampling with FSP-2, calculated together with samples of the two factories) to 89.7%. However there was no significant difference in dust concentrations by the two different samplers, 0.53±1.88 vs 0.73±1.61 mg/m(3) by FSP-2 and FSP-10 in the shipyard A and 1.14±1.78 vs 1.01±1.63 mg/m(3) in the factory B (t-test, P>0.05 in every case) . In addtion, sample loading by FSP-2 was found to be correlated to sampling time (R(2)=0.7906, y=0.002 6x) , therefore, it has to sample for ≥192.3 min to meet the LOD (0.5 mg) in case of normal flow rate. CONCLUSION: By using of high flow rate cyclone FSP-10 the problem of LOD could be solved, along with increased sample mass and similar respirable dust concentration by the two samplers. Some techincal improvements of FSP-10 and increasing of LOD coincidence rate by other methods was also disscussed.

A review on the research progress and application of compressed hydrogen in the marine hydrogen fuel cell power system.

The urgency to mitigate greenhouse gas emissions from maritime vessels has intensified due to the increasingly stringent directives set forth by the International Maritime Organization (IMO). These directives specifically address energy efficiency enhancements and emissions reduction within the shipping industry. In this context, hydrogen is the much sought after fuel for all the global economies and its applications, for transportation and propulsion in particular, is crucial for cutting down carbon emissions. Nevertheless, the realization of hydrogen-powered vessels is confronted by substantial technical hurdles that necessitate thorough examination. This study undertakes a comprehensive analysis encompassing diverse facets, including distinct variations of hydrogen fuel cells, hydrogen internal combustion engines, safety protocols associated with energy storage, as well as the array of policies and commercialization endeavors undertaken globally for the advancement of hydrogen-propelled ships. By amalgamating insights from these multifaceted dimensions, this paper adeptly encapsulates the myriad challenges intrinsic to the evolution of hydrogen-fueled maritime vessels, while concurrently casting a forward-looking gaze on their prospective trajectory.

Oligo(3,6-phenanthrene ethynylenes): synthesis, characterization, and photoluminescence.

A series of highly fluorescent, oligo(3,6-phenanthrene ethynylenes) (F1-F7) were synthesized, and their photophysical behavior was systematically investigated. They emitted light with highly emissive quantum yields, up to 0.92. Emissive wavelengths of these compounds relied on the number of phenanthrene blocks existing in the oligomers. Red-shifted emissions were observed as the number of phenanthrenes increased. On the basis of theoretical calculations, helical structures could be formed for F4-F7, indicating that the excimer emissions might be observed for F4-F7 due to the intramolecular π-π stackings of phenanthrenes in the helical structures. However, excimer emissions were only observed for F5-F7 in dilute cyclohexane and for F6 and F7 in dilute methylene chloride, respectively. No excimer emission was observed for F4-F7 in dilute tetrahydrofuran due to the degree of solvation.

Effects of magnesium on phosphorus chemical states and p-type conduction behavior of phosphorus-doped ZnO films.

Effects of magnesium on phosphorus chemical states and p-type conduction behavior of phosphorus-doped ZnO (ZnO:P) films were investigated by combining experiment with first-principles calculation. Photoluminescence (PL) spectra show that Mg incorporation increases the amount of V(Zn), which makes more P(Zn)-2V(Zn) complex acceptor formed and background electron density decreased, leading to that MgZnO:P exhibits better p-type conductivity than ZnO:P. The p-type conductivity mainly arises from P(Zn)-2V(Zn) complex acceptor with a shallow acceptor energy of 108 meV. X-ray photoelectron spectroscopy (XPS) spectra reveal that phosphorus has two chemical states of P(Zn)-2V(Zn) complex and isolated P(Zn), with binding energy of P(2p3∕2) of 132.81 and 133.87 eV, respectively. The conversion of isolated P(Zn) to P(Zn)-2V(Zn) complex induced by Mg incorporation is observed in XPS, in agreement with the PL results. First-principles calculations suggest that the formation energy of nMg(Zn)-V(Zn) complex decreases with the increasing Mg content, well supporting the experiments from the PL spectra and XPS measurements.

[Survey on the occupational musculoskeletal disorder and its risk factors among male steelworkers].

OBJECTIVE: To study the prevalence of occupational musculoskeletal disorders (OMSD) of male steelworkers and explore its occupational and individual risk factors. METHODS: 1620 male workers from a steel mill were selected as subjects through random cluster sampling in 2010. A revised Nordic Musculoskeletal disorder standard questionnaire and a questionnaire from National Institute for Occupational and Health of the Netherlands were used to ask and record the OMSD and its risk factors. The questionnaires were filled in by the workers. Annual prevalence of OMSD in different parts of the body were counted according to different age, working years and educational level, logistic regression was used to analyze its influence factors. RESULTS: OMSD in these workers primarily located in waist, neck and shoulders, annual prevalence were 51.0% (825), 48.5% (785) and 37.7% (610) respectively. Annual prevalence of other body parts were 28.0% (454) for back, 23.6% (383) for knee, 21.0% (341) for wrist, 17.9% (290) for ankle/foot, 14.1% (229) for elbow and 12.7% (205) for coxa. The annual prevalence of OMSD for each part tended to increase with age, mainly in waist and neck. Of the different age groups ≤ 24, 25 - 29, 30 - 34, 35 - 39, 40 - 44, 45 - 49, ≥ 50, the annual prevalence in waist was 26.4% (43/163), 37.8% (71/188), 52.8% (162/307), 55.6% (160/288), 53.8% (99/184), 55.9% (80/143), 61.6% (204/331) respectively (χ(2) = 72.5, P < 0.05); correspondingly, the annual prevalence in neck was 22.7% (37/163), 40.4% (76/188), 50.2% (154/307), 48.6% (140/288), 56.0% (103/184), 53.1% (76/143), 57.7% (191/331) respectively (χ(2) = 65.3, P < 0.05). The annual prevalence of OMSD increased with the working years. Of the different working years groups ≤ 4, 5 - 9, 10 - 14, 15 - 19, 20 - 24, 25 - 29, ≥ 30, the annual prevalence in waist was 30.2% (85/281), 46.2% (66/143), 56.4% (162/287), 56.8% (137/241), 50.6% (78/154), 59.2% (90/152), 60.7% (173/285) respectively (χ(2) = 71.3, P < 0.05);correspondingly, the annual prevalence in neck was 28.8% (81/281), 49.0% (70/143), 52.6% (151/287), 50.2% (121/241), 51.9% (80/154), 53.9% (82/152), 59.3% (169/285) respectively (χ(2) = 61.5, P < 0.05). The annual prevalence of OMSD decreased with education level. Of the different groups of education level (junior high school level and below, senior high school level, university level or above), the OMSD prevalence in waist was 61.5% (176/286), 61.9% (359/692), 44.2% (272/615) respectively (χ(2) = 26.0, P < 0.05);correspondingly, the annual prevalence in neck was 56.3% (161/286), 50.0% (346/692), 42.3% (260/615)respectively (χ(2) = 21.2, P < 0.05). Univariate logistic regression showed that the work load factors such as the working years ≥ 30 (OR = 3.562, 95%CI: 2.514 - 5.046), maintain substantial stoop for a long time (OR = 2.003, 95%CI: 1.612 - 2.488), often stooping with vast scale (OR = 1.897, 95%CI: 1.557 - 2.312), and torso repeating same action many times per minute (OR = 1.870, 95%CI: 1.529 - 2.288) could increase the annual prevalence of OMSD in waist most likely (P < 0.05). The working years ≥ 30 (OR = 3.597, 95%CI: 2.535 - 5.105), neck leaning forward (OR = 2.455, 95%CI: 2.010-2.99), neck leaning back (OR = 1.999, 95%CI: 1.569 - 2.546), and neck rotation (OR = 2.381, 95%CI: 1.907 - 2.972) were main risk factors causing OMSD in neck (P < 0.05). CONCLUSION: The most serious musculoskeletal disorders of male steelworkers were waist and neck pain. Personal factors such as age, working years, work load factors such as harmful working postures, manual heavy lifting, and labour organizational factors such as work overtime were the main risk factors of musculoskeletal disorders to the male steelworkers.

Improved Retinex algorithm for low illumination image enhancement in the chemical plant area.

Due to the complexity of the chemical plant area at night and the harsh lighting environment, the images obtained by monitoring equipment have issues such as blurred details and insufficient contrast, which is not conducive to the subsequent target detection work. A low illumination image enhancement model based on an improved Retinex algorithm is proposed to address the above issues. The model consists of a decomposition network, an adjustment network, and a reconstruction network. In the decomposition network, a new decomposition network USD-Net is established based on U-Net, which decomposes the original image into illumination and reflection maps, enhancing the extraction of image details and low-frequency information; Using an adjustment network to enhance the decomposed lighting image, and introducing a Mobilenetv3 lightweight network and residual structure to simplify the network model and improve the contrast of the image; In the reconstruction network, the BM3D method is used for image denoising to enhance the ability to restore image detail features; The enhanced illumination and reflection images were fused based on the Retinex algorithm to achieve low illumination image enhancement in the chemical plant area. This article uses five image quality evaluation indicators, namely Peak Signal-to-Noise Ratio, Structural Similarity Index, Natural Image Quality Evaluator, Interpolation Error, and Level of Effort, to compare eight traditional or modern algorithms and evaluate three different types of datasets. The experimental results show that the improved algorithm enhances the texture details of the image, improves the contrast and saturation of the image, and has good stability and robustness, which can effectively meet the needs of low illumination image enhancement in chemical plant areas.

Effects of Fumarate and Nitroglycerin on In Vitro Rumen Fermentation, Methane and Hydrogen Production, and on Microbiota.

This study aimed to investigate the effects of fumarate and nitroglycerin on rumen fermentation, methane and hydrogen production, and microbiota. In vitro rumen fermentation was used in this study with four treatment groups: control (CON), fumarate (FA), nitroglycerin (NG) and fumarate plus nitroglycerin (FN). Real-time PCR and 16S rRNA gene sequencing were used to analyze microbiota. The results showed that nitroglycerin completely inhibited methane production and that this resulted in hydrogen accumulation. Fumarate decreased the hydrogen accumulation and improved the rumen fermentation parameters. Fumarate increased the concentration of propionate and microbial crude protein, and decreased the ratio of acetate to propionate in FN. Fumarate, nitroglycerin and their combination did not affect the abundance of bacteria, protozoa and anaerobic fungi, but altered archaea. The PCoA showed that the bacterial (Anosim, R = 0.747, p = 0.001) and archaeal communities (Anosim, R = 0.410, p = 0.005) were different among the four treatments. Compared with CON, fumarate restored Bacteroidetes, Firmicutes, Spirochaetae, Actinobacteria, Unclassified Ruminococcaceae, Streptococcus, Treponema and Bifidobacterium in relative abundance in FN, but did not affect Succinivibrio, Ruminobacter and archaeal taxa. The results indicated that fumarate alleviated the depressed rumen fermentation caused by the inhibition of methanogenesis by nitroglycerin. This may potentially provide an alternative way to use these chemicals to mitigate methane emission in ruminants.

An efficient synthesis of heptaaryldipyrromethenes from tetraarylcyclopentadienones and ammonium acetate and their extension to the corresponding BODIPYs.

Heptaaryldipyrromethenes are efficiently prepared from ammonium acetate and tetraarylcyclopentadienones in a one-pot cascade process and can be converted into heptaaryl BODIPYs with fluorescent response to environmental acidity.

A comparative field study on dust measurements by different sampling methods with emphasis on estimating factors for recalculation from chinese 'total dust' measurements to respirable dust concentrations.

In China, dust samplers were originally designed to collect 'total dust' for a short term during production, which is different from the widely adopted sampling strategy for dust. With the aim to provide the conversion factor from Chinese total dust to US and German respirable dust and to look at the influences on conversion factors from environment, production, and instruments, a comparative field study on the dust concentration measurements by different sampling methods was carried out in the same Chinese industries as in the 1989-1990 study and in some other factories. A supplemental experiment was also conducted in a wind tunnel. Dust concentration was measured with a parallel sampling strategy by using the following samplers: 10-mm nylon cyclone for US respirable dust (AR), FSP-Berufsgenossenschaftliches Institut für Arbeitssicherheit (BIA) cyclone for German respirable dust (GR), and samplers for Chinese total dust (CT). Totally, 1434 samples were collected (269 AR, 198 GR, and 967 CT), from which 429 matched sample pairs (249 pairs of AR/CT, 180 GR/CT) were available to calculate conversion ratios. Industry- and job-based conversion factors are presented in this study. The conversion factor of AR/CT was 0.38 for tungsten mines, 0.19 for copper/iron mines, 0.65 for tin mines, and 0.20 for pottery industry, while the factor of GR/CT was 0.69 for tungsten, 0.37 for copper/iron, and 0.52 for pottery. In the job category, AR/CT factors varied from 0.16 to 0.96 and GR/CT from 0.12 to 0.72. For the industries studied in 1988-1989, the AR/CT and GR/CT factors were 0.29 and 0.45, respectively. Both factors were definitely influenced by production, CT dust concentration, sample gain, and variation of dust concentration. Moreover, the respirable dust concentration by FSP-BIA was significantly higher than that by 10-mm cyclones, 63.27-73.10% more as showed also by the wind tunnel experiment. Meanwhile, the GR/CT ratio was significantly larger than the AR/CT in every industry or job with only few exceptions. The GR/CT estimates should be considered as independent ones. Following these results, there is a need to use 'ideal samplers' (consistent with the internationally accepted respirable fraction) in practice and to assess the existent samplers in order to homogenize the exposure data situation.

Integration of Long Non-Coding RNA and mRNA Profiling Reveals the Mechanisms of Different Dietary NFC/NDF Ratios Induced Rumen Development in Calves.

The aim of the present study was to explore the effects of dietary non-fibrous carbohydrate to neutral detergent fiber (NFC/NDF) ratios on rumen development of calves, and to investigate the mechanisms by integrating of lncRNA and mRNA profiling. Forty-five weaned Charolais hybrid calves [body weight = 94.38 ± 2.50 kg; age = 70 ± 2.69 d] were randomly assigned to 1 of 3 treatment groups with different dietary NFC/NDF ratios: 1.10 (H group), 0.94 (M group) and 0.60 (L group), respectively. The ventral sac of the rumen was sampled for morphological observation and transcriptional sequencing. The average daily gain of calves in the high NFC/NDF ratio group was significantly higher than that in other groups (p < 0.05). Papillae width was largest in high NFC/NDF ratio group calves (p < 0.05). Identified differentially expressed genes that were significantly enriched in pathways closely related to rumen epithelial development included focal adhesion, Wingless-int signaling pathway, thyroid hormone signaling pathway, regulation of actin cytoskeleton and cGMP-PKG signaling pathway. The lncRNA-mRNA network included XLOC_068691 and MOAB, XLOC_023657 and DKK2, XLOC_064331 and PPP1R12A which we interpret to mean they have important regulatory roles in calve rumen development. These findings will serve as a theoretical basis for further analysis of the molecular genetic mechanism of dietary factors affecting rumen development in calves.

Effects of Different Roughages on Growth Performance, Nutrient Digestibility, Ruminal Fermentation, and Microbial Community in Weaned Holstein Calves.

This study aimed to assess the effects of feeding with different forage sources and starter concentrations on growth performance, nutrient digestibility, ruminal fermentation, and the microbial community in weaned Holstein calves. A total of 54 Holstein calves (body weight (BW) = 77.50 ± 5.07 kg; age = 70 ± 2.54 days) were assigned to 1 of 3 treatment groups (n = 18/group) that were offered diets with different forages: (1) peanut vine (PV), (2) oat hay (OH), or (3) an alfalfa hay + oat hay combination (alfalfa hay:oat hay =1:1, AO). Starter and forage intakes were recorded daily, while BW and growth parameters were assessed at 15-day intervals. The apparent digestibility of nutrients was determined. Ruminal fluid samples were collected and used to detect relevant indicators. A difference was observed for the forage × age interaction for all feed, nutrient intake, BW, ADG, and body structure parameters (P < 0.05). The final BW, average daily feed intake (ADFI), and average daily gain of the PV calves were higher than those of calves from the other groups (P < 0.05). The ruminal propionate concentration evidently increased in calves of the AO group (P < 0.05). The abundances of Rikenellaceae_RC9_gut_group and Shuttleworthia showed distinct responses to feeding with different forages (P < 0.05) at the genus level. The relative abundance of Shuttleworthia was negatively related to rumen pH and acid detergent fiber digestibility (P < 0.05) and strongly positively related to propionate concentration (P < 0.01). A positive correlation was found between Ruminococcus_1 abundance and butyrate concentration and neutral detergent fiber digestibility (P < 0.05). The relative abundances of Succiniclasticum and Prevotella_7 were negatively related to butyrate concentration (P < 0.05). In conclusion, there was an interaction between the factors (forage × age). The peanut vine used as a forage source promoted a higher starter concentrate intake compared to other diets and increased with the calves' age. The growth performance and rumen bacterial community of the calves were further improved. These results indicate that peanut vine can be used as the main source of forage in the diets of weaned calves.

Improved safety checklist analysis approach using intelligent video surveillance in the construction industry: a case study.

The construction industry is extremely high risk, and safety checklist analysis is a widely used approach for safety assessment. To overcome its limitations, this article proposes an improved safety checklist analysis approach using intelligent video surveillance to replace on-site inspection. Then, a case study on metro tunnel construction is adopted to illustrate the process. First, the checklist is prepared. Second, the inspection items are correlated with construction areas, and intelligent cameras are positioned to cover the major areas of the construction site to guarantee that all the items can be checked. Thus, problems with inspection items are automatically identified and recorded. Third, the inspection items are marked by a remote scoring mechanism for safety assessment. Finally, the efficiency of the improved approach is tested by a comparative analysis among three groups. The application results indicate the feasibility of the improved approach for evaluating the safety management performance at construction sites.

8-Methoxypsoralen has Anti-inflammatory and Antioxidant Roles in Osteoarthritis Through SIRT1/NF-κB Pathway.

Osteoarthritis (OA) is mainly manifested by joint pain, stiffness and mobility disorder, which is the main cause of pain and disability in middle-aged and elderly people. In this study, we aimed to explore the role and mechanism of 8-Methoxypsoralen (8-MOP) in the OA model both in vitro and in vivo. The rat chondrocytes were treated with IL-1ß, and the proliferation, apoptosis, inflammatory reactions and oxidative stress responses were determined after treatment with different concentrations of 8-MOP. Real-time quantitative polymerase chain reaction (qRT-PCR) and/or Western blot were implemented to check the AMPK/SIRT1/NF-κB expression in chondrocytes. The NF-κB activity was determined by dual luciferase experiment. The pain threshold of OA rat model dealt with 8-MOP and/or the SIRT1 inhibitor EX527 was measured. Our results revealed that 8-MOP evidently reduced IL-1ß-mediated apoptosis and inhibition of proliferation, and mitigated the expression of inflammatory cytokines and oxidative stress factors in chondrocytes. Additionally, 8-MOP promoted phosphorylated level of AMPKα, enhanced SIRT1 expression and inhibited the phosphorylation of NF-κB. After treatment with EX527, 8-MOP-mediated protective effects on chondrocytes were mostly reversed. In vivo, 8-MOP obviously improved the pain threshold in the OA rat model and reduced the injury and apoptosis of chondrocytes in the joints. In addition, 8-MOP relieved inflammatory and oxidative stress responses in the articular cartilage via enhancing SIRT1 and repressing NF-κB activation. After the treatment with EX527, the 8-MOP-mediated protective effects were distinctly weakened. In summary, our study testified that 8-MOP alleviates pain, inflammatory and oxidative stress responses in OA rats through the SIRT1/NF-κB pathway, which is expected to become a new reagent for clinical treatment of OA.