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1.
Inorg Chem ; 62(41): 16669-16672, 2023 Oct 16.
Article in English | MEDLINE | ID: mdl-37795820

ABSTRACT

Studies about the reaction of ZrIV ions with peroxides and the properties of the resulting zirconium peroxide clusters are significant for understanding zirconium chemistry in the nuclear fuel cycle and the advancement of less explored Group IV metal oxo clusters. Herein, an octanuclear zirconium peroxide cluster, designated as Zr8, was synthesized and characterized by using multiple techniques. Crystallographic analysis revealed that Zr8 has a ringlike structure and unusual positive charges, while tetravalent metal oxo clusters are mostly neutral. In situ variable-temperature Raman spectra indicated that Zr8 has unexpected thermal stability, which may be related to the strong interaction between ZrIV ions and peroxide groups. Small-angle X-ray scattering data showed that Zr8 self-assembled in the reactant solution prior to crystallization. In short, Zr8 expands the limited family of zirconium peroxide clusters and enriches the properties of metal peroxides.

2.
Biomed Chromatogr ; 36(6): e5358, 2022 Jun.
Article in English | MEDLINE | ID: mdl-35187696

ABSTRACT

A UHPLC-MS/MS method for the quantification of ADP355, an adiponectin-derived active peptide, was developed and validated. The extraction method employed simple protein precipitation using methanol and chromatographic separation was achieved on anAccucore™ RP-MS C18 column (100 × 2.1 mm, 2.6 µm, 80 Å), using 0.1% formic acid in both water and acetonitrile with gradient elution at the flow rate of 400 µl/min within 4.0 min. Detections were performed under positive ion mode with multiple reaction monitoring ion transitions m/z 1109.2 → 309.8 and 871.4 → 310.1 for ADP355 and Jt003 respectively at unit resolution. The linearity range of the calibration curve was 2-1,000 ng/ml with a lower limit detection of 0.5 ng/ml. The selectivity, linearity, precision, accuracy, recovery, matrix effect and stability were validated, and all items met the requirement of US Food and Drug Administration guidance. This method was successfully applied to an intravenous pharmacokinetic study of ADP355 in rats and the in-vitro stability in rat serum, plasma and whole blood was also assessed.


Subject(s)
Adiponectin , Chromatography, High Pressure Liquid , Oligopeptides , Tandem Mass Spectrometry , Adiponectin/blood , Animals , Chromatography, High Pressure Liquid/methods , Oligopeptides/blood , Rats , Rats, Sprague-Dawley , Reproducibility of Results , Tandem Mass Spectrometry/methods
3.
Anal Chem ; 92(11): 7690-7698, 2020 06 02.
Article in English | MEDLINE | ID: mdl-32392405

ABSTRACT

The present project studied the signal drift in liquid chromatography tandem mass spectrometry (LC-MS/MS) and proposed a strategy for compensating such drift. In the study, four 4-component groups were repeatedly run on different LC-MS/MS systems for over 12 h to investigate the dependence of signal drift on time and hardware systems. The 4-component groups each consisted of (1) an analyte, (2) a stable isotope labeled analyte, (3) a compound with similar structure to the analyte, and (4) a compound with dissimilar structure. All of the species showed significant signal drift, generally more than 25% over 12 h. The analyte and its stable isotope labeled analog always have the same drifting pattern including the trends and direction from one LC-MS/MS system to another. Signal drift was also found to be concentration dependent. Our experiments further proved that a conventional stable isotope labeled internal standard in LC-MS/MS quantification would not compensate the variations caused by concentration-dependent signal drift. An ideal internal standard for LC-MS/MS has both identical structure and similar concentration to the analyte. For that, we proposed a new internal standard strategy, pseudo internal standard (Pseudo IS), for LC-MS/MS quantification. Pseudo IS could effectively compensate signal drift in spite of its significant time, system, and concentration dependencies.

4.
Macromol Rapid Commun ; 35(2): 133-140, 2014 Jan.
Article in English | MEDLINE | ID: mdl-24150838

ABSTRACT

Quality of gradient copolymers is evaluated by atomic force microscopy (AFM) and correlated with molecular weight distribution (MWD) values. ARGET ATRP is employed with decreasing levels of catalyst concentrations to generate copolymers with increasing M¯w/M¯n values. The copolymers are transformed into molecular bottlebrushes to enable imaging and analysis of individual molecules by AFM. The average height (cross-sectional) profile of all bottlebrushes agrees with the instantaneous composition (ICHEMA-TMS ) of the analogous copolymer backbone, as determined by (1) H NMR. The copolymer synthesized with 500 ppm of catalyst exhibits more narrow distributions of both brush height and backbone length when analyzed as a bottlebrush by AFM. Correspondingly, the copolymers synthesized with lower catalyst concentrations yield bottlebrushes with broader height and length distribution. These results establish MWD values as an excellent trait to assess quality within gradient copolymers.


Subject(s)
Polymers/chemistry , Magnetic Resonance Spectroscopy , Microscopy, Atomic Force , Molecular Weight
5.
J Pharm Sci ; 113(8): 2605-2615, 2024 Aug.
Article in English | MEDLINE | ID: mdl-38460573

ABSTRACT

BACKGROUND: Cefotaxime is commonly used in treating bacterial infections in neonates. To characterize the pharmacokinetic process in neonates and evaluate different recommended dosing schedules of cefotaxime, a physiologically-based pharmacokinetic (PBPK) model of cefotaxime was established in adults and scaled to neonates. METHODS: A whole-body PBPK model was built in PK-SIM® software. Three elimination pathways are composed of enzymatic metabolism in the liver, passive filtration through glomerulus, and active tubular secretion mediated by renal transporters. The ontogeny information was applied to account for age-related changes in cefotaxime pharmacokinetics. The established models were verified with realistic clinical data in adults and pediatric populations. Simulations in neonates were conducted and 100 % of the dosing interval where the unbound concentration in plasma was above the minimum inhibitory concentration (fT>MIC) was selected as the target index for dosing regimen evaluation. RESULTS: The developed PBPK models successfully described the pharmacokinetic process of cefotaxime in adults and were scaled to the pediatric population. Good verification results were achieved in both adults' and neonates' PBPK models, indicating a good predictive performance. The optimal dosage regimen of cefotaxime was proposed according to the postnatal age (PNA) and gestational age (GA) of neonates. For preterm neonates (GA < 36 weeks), dosages of 25 mg/kg every 8 h in PNA 0-6 days and 25 mg/kg every 6 h in PNA 7-28 days were suggested. For term neonates (GA ≥ 36 weeks), dosages of 33 mg/kg every 8 h in PNA 0-6 days and 33 mg/kg every 6 h in PNA 7-28 days were recommended. CONCLUSIONS: Our study may provide useful experience in practicing PBPK model-informed precision dosing in the pediatric population.


Subject(s)
Anti-Bacterial Agents , Cefotaxime , Infant, Premature , Models, Biological , Humans , Cefotaxime/pharmacokinetics , Cefotaxime/administration & dosage , Infant, Newborn , Anti-Bacterial Agents/pharmacokinetics , Anti-Bacterial Agents/administration & dosage , Adult , Computer Simulation , Female , Male , Microbial Sensitivity Tests , Gestational Age
6.
J Clin Pharmacol ; 2024 Sep 03.
Article in English | MEDLINE | ID: mdl-39223982

ABSTRACT

Vancomycin has a narrow therapeutic window and a high inter-individual pharmacokinetic variability, especially in neonates with fast maturational and pathophysiological changes, that needs individualized dosing. Physiologically based pharmacokinetic (PBPK) model and population pharmacokinetic (PopPK) model are both useful tools in model-informed precision dosing, while the former is under research in application of vancomycin in neonates. This study aimed to develop a PBPK model of vancomycin in adult and pediatric population, and compared it with published PopPK model (priori or Bayesian method) in predicting vancomycin concentration in 230 neonatal patients (postmenstrual age, PMA, 25-45 weeks). The developed PBPK model showed a good fit between predictions and observations. PBPK model and PopPK model are complementary in different clinical scenarios of vancomycin application. The physiological-change description of PBPK model showed a superior advantage in initial dosing optimization. As for subsequent dose optimization, PopPK Bayesian forecasting performed better than the PBPK estimation in neonates. However, initial precision dosing tools for early neonates (with PMA < 36 weeks) still need further exploitation.

7.
J Am Soc Mass Spectrom ; 34(7): 1295-1304, 2023 Jul 05.
Article in English | MEDLINE | ID: mdl-37338210

ABSTRACT

Recently, we developed a novel microprobe electrospray ionization (µPESI) source and its coupled MS (µPESI-MS/MS) system. Here, we aimed to widely validate the µPESI-MS/MS method for quantitative analysis of drugs in plasma samples. Furthermore, the relationship between the quantitative performance of the µPESI-MS/MS method and the physicochemical properties of target drugs was analyzed. The µPESI-MS/MS methods for quantitative analysis of 5 representative drugs with a relatively wide range of molecular weight, pKa, and log P values were developed and validated. The results showed that the linearity, accuracy, and precision of these methods met the requirements of the European Medicines Agency (EMA) guidance. Then a total of 75 drugs from plasma samples were primarily detected using the µPESI-MS/MS methods, among which 48 drugs could be quantitatively measured. Logistics regression suggested that drugs with significantly greater log P and physiological charge had better quantitative performance using the µPESI-MS/MS method. Collectively, these results clearly demonstrate the practical application of the µPESI-MS/MS system as a rapid approach to the quantitative analysis of drugs in plasma samples.


Subject(s)
Spectrometry, Mass, Electrospray Ionization , Tandem Mass Spectrometry , Tandem Mass Spectrometry/methods , Spectrometry, Mass, Electrospray Ionization/methods , Drug Delivery Systems , Chromatography, High Pressure Liquid/methods
8.
Adv Mater ; 32(50): e2005314, 2020 Dec.
Article in English | MEDLINE | ID: mdl-33176030

ABSTRACT

The ability of living species to transition between rigid and flexible shapes represents one of their survival mechanisms, which has been adopted by various human technologies. Such transition is especially desired in medical devices as rigidity facilitates the implantation process, while flexibility and softness favor biocompatibility with surrounding tissue. Traditional thermoplastics cannot match soft tissue mechanics, while gels leach into the body and alter their properties over time. Here, a single-component system with an unprecedented drop of Young's modulus by up to six orders of magnitude from the GPa to kPa level at a controlled temperature within 28-43 °C is demonstrated. This approach is based on brush-like polymer networks with crystallizable side chains, e.g., poly(valerolactone), affording independent control of melting temperature and Young's modulus by concurrently altering side chain length and crosslink density. Softening down to the tissue level at the physiological temperature allows the design of tissue-adaptive implants that can be inserted as rigid devices followed by matching the surrounding tissue mechanics at body temperature. This transition also enables thermally triggered release of embedded drugs for anti-inflammatory treatment.


Subject(s)
Smart Materials , Transition Temperature , Elastic Modulus , Materials Testing
9.
ACS Appl Mater Interfaces ; 10(24): 20869-20875, 2018 Jun 20.
Article in English | MEDLINE | ID: mdl-29790739

ABSTRACT

Multifunctional coatings that adhere to chemically distinct substrates are vital in many industries, including automotive, aerospace, shipbuilding, construction, petrochemical, biomedical, and pharmaceutical. We design well-defined, nearly monodisperse microgels that integrate hydrophobic dopamine methacrylamide monomers and hydrophilic zwitterionic monomers. The dopamine functionalities operate as both intraparticle cross-linkers and interfacial binders, respectively providing mechanical strength of the coatings and their strong adhesion to different substrates. In tandem, the zwitterionic moieties enable surface hydration to empower antifouling and antifogging properties. Drop-casting of microgel suspensions in ambient as well as humid environments facilitates rapid film formation and tunable roughness through regulation of cross-linking density and deposition conditions.

10.
Adv Mater ; 29(2)2017 Jan.
Article in English | MEDLINE | ID: mdl-27859735

ABSTRACT

Freestanding, single-component dielectric actuators are designed based on bottlebrush elastomers that enable giant reversible strokes at relatively low electric fields and altogether avoid preactuation mechanical manipulation. This materials design platform allows for independent tuning of actuator rigidity and elasticity over broad ranges without changing chemical composition, which opens new opportunities in soft-matter robotics.

11.
Nat Commun ; 7: 12919, 2016 Sep 27.
Article in English | MEDLINE | ID: mdl-27676123

ABSTRACT

Shapeshifting enables a wide range of engineering and biomedical applications, but until now transformations have required external triggers. This prerequisite limits viability in closed or inert systems and puts forward the challenge of developing materials with intrinsically encoded shape evolution. Herein we demonstrate programmable shape-memory materials that perform a sequence of encoded actuations under constant environment conditions without using an external trigger. We employ dual network hydrogels: in the first network, covalent crosslinks are introduced for elastic energy storage, and in the second one, temporary hydrogen-bonds regulate the energy release rate. Through strain-induced and time-dependent reorganization of the reversible hydrogen-bonds, this dual network allows for encoding both the rate and pathway of shape transformations on timescales from seconds to hours. This generic mechanism for programming trigger-free shapeshifting opens new ways to design autonomous actuators, drug-release systems and active implants.

12.
Adv Mater ; 27(43): 6899-905, 2015 Nov 18.
Article in English | MEDLINE | ID: mdl-26436409

ABSTRACT

A new type of "rigid and tough" hydrogel with excellent elasticity is designed by dense clustering of hydrogen bonds within a loose chemical network. The resultant hydrogel exhibits a good combination of high modulus (28 MPa), toughness (9300 J m(-3) ), extensibility (800%), and tensile stress (2 MPa). Furthermore, the gel displays good fatigue-resistance and complete and extremely fast recovery of shape and mechanical properties (3 min at 37°C).

13.
ACS Appl Mater Interfaces ; 7(26): 14288-93, 2015 Jul 08.
Article in English | MEDLINE | ID: mdl-26081101

ABSTRACT

Shape memory polymers (SMPs) have been shown to accurately replicate photonic structures that produce tunable optical responses, but in practice, these responses are limited by the irreversibility of conventional shape memory processes. Here, we report the intensity modulation of a diffraction grating utilizing two-way reversible shape changes. Reversible shifting of the grating height was accomplished through partial melting and recrystallization of semicrystalline poly(octylene adipate). The concurrent variations of the grating shape and diffraction intensity were monitored via atomic force microscopy and first order diffraction measurements, respectively. A maximum reversibility of the diffraction intensity of 36% was repeatable over multiple cycles. To that end, the reversible shape memory process is shown to broaden the functionality of SMP-based optical devices.

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