Electrochemical selenocyclization of 2-ethynylanilines with diselenides: facile and efficient access to 3-selenylindoles.

An efficient electrochemical selenocyclization strategy for the synthesis of 3-selenylindoles from 2-ethynylanilines and diselenides has been developed in simple tube- or beaker-type undivided cells under ambient conditions. Notably, these sustainable transformations are completed within a short time with low equivalents of charges, diselenides and electrolytes, exhibiting a broad substrate scope with excellent functional group compatibility. Moreover, a gram-scale electrosynthesis and late-stage functionalization of complex molecules further demonstrate the practical synthetic potential of this facile electrochemical system.

Rational design of biogenic PdxAuy nanoparticles with enhanced catalytic performance for electrocatalysis and azo dyes degradation.

The green biogenic PdAu nanoparticles (bio-PdAu NPs) exhibits remarkable catalytic performance in hydrogenation, which is highly desired. However, the catalytic principles and effectiveness of bio-PdxAuy NPs in response to various catalytic systems (electrocatalysis and suspension-catalysis) are unclear. Herein, a facile synthetic strategy for bio-PdxAuy NPs synthesis with controlled size and the catalytic principles for hydrogen evolution reaction (HER) and azo dye degradation is reported. In the biosynthetic process, the size and composition of the bio-PdxAuy NPs could be precisely controlled by predesigning the precursor mass ratio of Pd/Au, and the Au proportion showed a linear relationship with the size of NPs (R2 = 0.92). The obtained bio-PdxAuy NPs exhibit variable activity in electrocatalysis (HER) and suspension-catalysis (azo dye degradation). For electrocatalysis, the formation of conductive networks that facilitates the extracellular electron transfer is crucial. It was revealed that the bio-Pd2Au8 exhibited superior electrocatalytic performance in HER/toward hydrogen evolution, with a maximum current density of 1.65 mA cm-2, which was 1.54 times higher than that commercial Pd/C (1.07 mA cm-2). The high electrocatalytic activity was attributed to its appropriate size (81.38 ± 6.14 nm) and uniform distribution on the cell surface, which promoted the extracellular electron transfer by constructing a conductive network between catalyst and electrode. However, for suspension-catalysis, the size effect and synergistic effect of bimetallic NPs have a more prominent effect on the degradation of azo dyes. As the increase of Au proportion the particle size decreases, and the catalytic activity of bio-PdxAuy improved significantly. The response principles of bio-PdxAuy proposed in this study provide a reliable reference for the rational design of bio-based bimetallic catalysts with enhanced catalytic performance.

Semi-starvation fluctuation driving rapid partial denitrification granular sludge cultivation in situ by microorganism exudate metabolites feedbacks.

In this study, semi-starvation fluctuation driving PD granules cultivation in situ by microorganism exudate metabolites feedbacks was firstly investigated. The PD granules of high nitrite production were cultivated with an excellent mean nitrate-to-nitrite transformation rate (NTR) of 56.39% in just 30 days. The granules size was improved from the initial size of 0.09 ± 0.01 mm in diameter to a size above 2 mm when the extracellular polymeric substance (EPS) content increased from 80.21 ± 10.20 mg/g MLVSS to 777.00 ± 22.13 mg/g MLVSS. Acyl-homoserine lactone signals (AHLs) ultimately increased ten-fold more than the initially through 30 days of cultivation. Meanwhile, Thauera had been identified as the main function bacteria of PD, which enriched from 0.47% to 10.67%. Results demonstrated that AHLs, EPS, PD bacteria and the PD granules cultivation were closely associated. Semi-starvation fluctuation produced oligotrophic stress on bacterial community, a part of bacteria would be eliminated on starvation for oligotrophic stress and AHLs of bacteria regarded as distress signals resulted in the rapid formation of PD granules. A mechanism for PD granular cultivation with semi-starvation fluctuation was proposed from the aspect of oligotrophic stress. A better strategy for rapid PD granules cultivation was obtained and it could be useful for the mainstream granule-based PD combined with the anammox process application in the future.

Effect of calcinated pyrite on simultaneous ammonia, nitrate and phosphorus removal in the BAF system and the Fe2+ regulatory mechanisms: Electron transfer and biofilm properties.

To efficiently remove nitrogen and phosphorus from secondary effluent with low values of COD/TN, a novel biological aerated filter (BAF) utilizing calcined pyrite with a large specific surface area (SSA) and pore diameter (PD) was designed to address this challenge. From the perspective of nutrients removal performance, and the corresponding effluent total nitrogen (TN) and PO43--P in the calcined pyrite autotrophic denitrification (CPAD) process decreased from 40.21 to 1.07 mg/L to 1.22 and 0.14 mg/L, respectively. Furthermore, the nutrients removal kinetics analysis showed that the CPAD and pyrite autotrophic denitrification (PAD) processes could be fitted with Half-order and Zero-order reactions via kinetics analysis, respectively, indicating that the TN removal performance of CPAD processes was better than that of the PAD process. Moreover, CPAD combined with sulfur autotrophic denitrification (SAD) processes was fitted by First-order reaction, and the TN removal performance was further enhanced over the CPAD process. From the perspective of microregulation, Fe2+ production in the PAD and CPAD processes could accelerate the electron transfer rate by increasing electron transport system activity (ETSA) and reducing electrochemical impedance spectroscopy (EIS). Moreover, Fe2+ stimulated microbes to produce more proteins (PN) and C10-HSL, which improved biofilm stability and interspecific communication processes. Notably, nitrifiers and autotrophic denitrifiers were simultaneously enriched via detection of high-throughput sequencing of 16 S rRNA genes, which verified the feasibility of simultaneous nitrification and autotrophic denitrification. Therefore, BAF with calcined pyrite and sulfur as composite fillers have a considerable advantage in nutrients removal.

Enhanced Degradation of Levofloxacin through Visible-Light-Driven Peroxymonosulfate Activation over CuInS2/g-C3N4 Heterojunctions.

In this work, the heterojunctions of CuInS2 embedded in the g-C3N4 materials (xCuInS2/g-C3N4, abbreviated as xCIS/GCN) was successfully prepared for peroxymonosulfate (PMS) activation under visible light. The catalysts are characterized by different techniques, such as XRD, FTIR, SEM, TEM, and UV-vis. The unique heterojunction composites can suppress the recombination of photogenerated pairs. The catalytic results showed that the 3CIS/GCN exhibited excellent catalytic levofloxacin (LVF) degradation efficiency, while more than 98.9% of LVF was removed in 60 min over a wide pH range. SO4•-, O2•-, OH•, and 1O2 were verified as the main reactive species for LVF degradation via the quenching experiments and electron paramagnetic resonance technology (EPR). The synergetic effect of xCIS/GCN, PMS, and visible light irradiation was discussed. The possible LVF degradation pathway was proposed through byproducts analysis (LC-MS). Moreover, the 3CIS/GCN/vis-PMS system has very low metal leaching. Owing to xCIS/GCN having good properties for PMS activation, it has potential applications for LVF or other hazardous pollutants degradation.

Multifaceted synergistic facilitation mechanism of conductive polymers in promoting selenite bioreduction and biological detoxification.

Here, polypyrrole (PPY) was first used to the bioreduction of toxic selenite, while the acceleration effect and mechanism were explored. Experiment results suggested that PPY could enhance the selenite bioreduction from 0.42 to 1.04 mg/(L·h). The tests of electrochemical analysis and cytochrome c (cyt-c) content confirmed that PPY promoted the intracellular/intracellular electron transfer of Shewanella oneidensis·MR-1 in selenite bioreduction process. The enhancement of metabolic activity by PPY contributed to biological detoxification, which was manifested in the increased extracellular polymeric substances (EPS), adenosine triphosphate (ATP), electron transfer system activity (ETSA), membrane permeability and enzyme activity. Transcriptome analysis of DEGs, KEGG pathway enrichment and GO functional classification verified that the environmental adaptability of Shewanella oneidensis·MR-1 was enhanced with the addition of PPY. The transmission electron microscopy (TEM) images indicated that PPY promoted the biosynthesis of selenium nanoparticles (SeNPs), which was beneficial to reduce cell damage. Combined with the above results, a multifaceted synergistic facilitation mechanism based on "conductive cross-linking network" was elaborated from electron transfer, microbial metabolism and environmental adaptability. This study shed light the effect of conductive polymers (CPs) on selenite bioreduction and provided new insights into the bioremediation of toxic pollutants.

Insight into the enhancing mechanism of silica nanoparticles on denitrification: Effect on electron transfer and microbial metabolism.

Although silica nanoparticles (SiNPs) are produced in large numbers for industrial manufacturing and engineering applications, the effect of SiNPs on biotransformation in the environment is still not clear. In the current study, the effect of SiNPs in enhancing denitrification was investigated, and its mechanism was explored from the perspectives of electron transfer, microbial metabolism and bacterial community structure for the first time. Batch experiments showed that a concentration of SiNPs ranging from 0.05 to 5 g/L enhanced the bioreduction of nitrate. The mechanism study showed that SiNPs accelerated the extracellular electron transfer in the denitrification process due to their electron donating capacity, bonding action, and the secretion of more electron shuttles. During the denitrification process, SiNPs promoted metabolic activity, which mainly consists of promoting enzyme activities and electron transport system activity; these metabolic activity assays were positively correlated with SiNPs according to the structural equation modeling analysis. Moreover, SiNPs affected the composition of the microbial community, including denitrifying functional bacteria, silicon-activating bacteria and electron transfer active bacteria exhibiting a synergistic symbiosis. In addition, it was shown, by investigating two functional group-modified SiNPs, that the carboxyl modified SiNPs had the potential to be applied in nitrogen removal due to their performance and non-toxicity. This study presented a better insight into the role of SiNPs in biological transformation.

Te(IV) bioreduction in the sulfur autotrophic reactor: Performance, kinetics and synergistic mechanism.

A laboratory-scale sulfur autotrophic reactor (SAR) was first constructed for treating tellurite [Te(IV)] wastewater. The SAR had excellent Te(IV) bioreduction efficiency (90-96%) at 5-30 mg/L and conformed to the First-order kinetic model. The Te(IV) bioreduction was elaborated deeply from extracellular polymeric substances (EPS) functions, microbial metabolic activity, key enzyme activity, microbial community succession and quorum sensing. Te(IV) stimulated the increase of redox substances in EPS and the improved cell membrane permeability led to the increase of electron transport system activity. Catalase and reduced nicotinamide adenine dinucleotide (NADH) alleviated the oxidative stress caused by Te(IV) toxicity to maintain metabolic activity. The increase of sulfur dioxygenase activity (SDO) suggested that more ATP produced by sulfur oxidation might provide energy for various physiological activities. Meanwhile, nitrate reductase (NAR), nitrite reductase (NIR) and sulfide: quinone oxidoreductase (SQR) played an active role in sulfur oxidation and Te(IV) bioreduction. Combined with the above results and dynamic succession of three functional microbial communities, a synergistic mechanism was proposed to explain the excellent performance of SAR. This work provided a promising strategy for Te(IV) wastewater treatment process and Te(IV) bioreduction mechanism.

Deciphering effects of humic acid in landfill leachate on the simultaneous nitrification, anammox and denitrification (SNAD) system from performance, electron transfer and microbial community.

Simultaneous nitrification, anammox and denitrification (SNAD) system is effective for landfill leachate treatment. However, humic acid (HA) as both an organic pollutant and electron shuttle in landfill leachate, its effects on the SNAD system remain unknown. This study demonstrated that HA initially inhibited NH4+-N removal efficiency due to HA inhibition on anammox bacteria (the lowest fell to 90.89% from 100%), but the HA inhibition was released after adaption in the SNAD system. Hence, the mechanism of releasing HA inhibition in the SNAD system was established from performance, electron transfer and microbial community. Firstly, HA could be effectively removed by an adsorption-biodegradation process in the SNAD system, which avoided deteriorated performance caused by HA accumulation. Electrochemical analysis demonstrated that HA stimulated riboflavin and flavin mononucleotide (FMN) secretion to promote electron transfer efficiency. With the improved electron transfer efficiency, ETSA and ATP values significantly increased, indicating that HA enhanced the microbial metabolism activity of the SNAD system. Further analysis by enzymatic activity assay showed that the HAO (39.68 to 69.53 U/L), AMO (242.94 to 308.36 U/L), HZO (133.73 to 169.65 U/mL), NXR (24.63 to 54.52 U/L), NAR (94.40 to 114.36 U/L) and NIR (104.40 to 123.74 U/L) activities were improved with the HA increased from 0 to 200 mg/L, manifesting that HA enhanced nitrogen metabolism in the SNAD system. Besides, more reasonable metabolic division of labor in functional bacterial and enrichment of heterotrophic bacteria achieved efficient simultaneous removal of HA and nitrogen. Overall, efficient HA biodegradation, faster electron transfer efficiency and better metabolic division of microbial communities released HA inhibition, making the SNAD system more resistant to HA stress. This study shed light on the effects of HA on the SNAD system and provided a new insight for the SNAD system to landfill leachate treatment.

Multifaceted synergistic electron transfer mechanism for enhancing denitrification by clay minerals.

The performance and mechanism of denitrification enhanced by three clay minerals, montmorillonite (Mmt), illite and kaolinite, were first studied. Batch experiments indicated that clay minerals significantly enhanced denitrification at certain concentrations (0.1-1 g/L). The denitrification rate with 1 g/L Mmt was increased by 5.0-fold. The mechanism of clay minerals promoting denitrification was analyzed from three aspects: electron transfer characteristics, interfacial interaction and metabolism activity. Electrochemical tests showed that the clay minerals promoted electron transfer rate by improving current efficiency and electronic accommodation capacity. The biofilm formation on the clay minerals interface indicated that micro-domain catalytic phases were formed, which was beneficial to improve the nitrate reduction rate. In addition, nicotinamide adenine dinucleotide, nitrate reductase and nitrite reductase activities in Mmt-supplemented system were increased by 283.3%, 128.1% and 126.2%, respectively; and extracellular polymeric substance secretion was enhanced, indicating that the addition of clay minerals promoted microbial metabolic activity. Higher microbial diversity and enrichment of electroactive bacteria were observed in the Mmt-supplemented system. Based on the above exploration, the multifaceted synergistic mechanism was proposed to account for the enhanced denitrification performance on clay minerals. Overall, this study expanded understanding of the roles of clay minerals on denitrification and provided strategies for accelerating the biological transformation process.

Bioinspired facilitation of intrinsically conductive polymers: Mediating intra/extracellular electron transfer and microbial metabolism in denitrification.

Intrinsically conductive polymers, polyaniline and polyaniline sulfonate (PASAni) were used to explore their effect on denitrification. Denitrification was accelerated 1.90 times by 2 mM PASAni and the possible mechanisms were mainly attributed to the accelerated electron transfer and the enhanced microbial metabolism activity. Intracellular electron transfer was accelerated by PASAni and the acceleration sites were from NADH to coenzyme Q (CoQ), quinone loop, from Complex II to CoQ and from QH2 to Cyt. c1. Extracellular electron transfer was accelerated because PASAni promoted more secretion of redox species and PASAni embedded in extracellular polymeric substance (EPS). Moreover, PASAni itselfprovided more electron transfer pathways as redox species. Microbial metabolism activity was also enhanced by PASAni, which was reflected in the increased nitrate/nitrite reductase activity (236.13/155.43%), electron transfer system activity (112.49%), adenosine triphosphate level (133.41%) and EPS content (189.06%). Besides, the enriched Proteobacteria in PASAni supplement system was also conducive to denitrification. This work provided fundamental information for conductive polymers mediating microbial electron transfer and enhancing contaminants biotransformation.

Formation of anaerobic granular sludge (AnGS) to treat high-strength perchlorate wastewater via anaerobic baffled reactor (ABR) system: Electron transfer characteristic, bacterial community and positive feedback mechanism.

Anaerobic granular sludge (AnGS) was cultured to treat high-strength perchlorate (reaching to 4800 mg/L) wastewater by an anaerobic baffled reactor (ABR) system with five equal-volume compartments (C1-C5 compartments). Inoculated sludge completely granulated on day 104 with granule size of 0.50-0.75 mm and perchlorate removal efficiency reaching to 97% (influent perchlorate of 2000-4800 mg/L). The Cyclic voltammetry (CV) capacitance increased from 487.5, 465.8 and 407.8 µF to 576.5, 552.4, 549.6 µF in C1, C3 and C5 compartments of ABR system, respectively, suggesting the electron transfer capacity was enhanced under high-strength perchlorate stress. Meanwhile, adenosine triphosphate (ATP) value and electron transport system activity (ETSA) increased to 25.05, 22.87, 20.43 and 6.22, 4.87, 3.95 of C1, C3 and C5 compartments, respectively. The results suggested that high-strength perchlorate stress improved the microbial metabolic activity, which promoted secretion of extracellular polymeric substances (EPS). The more EPS could facilitate the formation and stability of AnGS under high-strength perchlorate stress. In addition, more reasonable metabolic division of labor in functional bacterial (Thauera and Comamonas) was beneficial to AnGS formation, which achieved high-strength perchlorate efficient removal. Finally, a positive feedback mechanism between AnGS formation and high-strength perchlorate removal was established through EPS, microbial metabolic activity and electron transfer characteristic in ABR system. However, excessive perchlorate (5800 mg/L) would exceed the treatment capacity of AnGS, which resulted in the deterioration of removal performance. This work provided an effective information for AnGS application to treat high-strength perchlorate wastewater.

Perchlorate bioreduction in UASB reactor: S2--autotrophic granular sludge formation and sulphate generation control.

Perchlorate (ClO4-) industrial wastewater requires efficient removal to prevent adverse environmental impacts, however, high concentration and low biodegradability give rise to poor ClO4- bioreduction performance. S2--autotrophic granular sludge (S2--AuGS) was firstly cultivated for high concentration perchlorate (ClO4-) removal in the upflow anaerobic sludge blanket (UASB) reactor (ClO4-: 150 mg L-1). Simultaneously, the S2- was utilized to control the SO42- generation as electron donor, the effluent SO42- concentration (190 mg L-1) was satisfied with drinking water standard (250 mg L-1). Under the optimized condition of hydraulic retention time (HRT) (6 h) and S2-/ClO4- molar ratio (2.2), more EPS was secreted, which promoted the S2--AuGS formation and stability. Though acclimation of 146 d, the S2--AuGS was formed with a large average granular sludge size (612 µm) and an excellent settleability (sludge volume index: SVI5/SVI30 = 1). With the mature S2--AuGS formation, the highest ClO4- and S2- loading was increased to 1.06 and 0.75 kg m-3 d-1. Interestingly, Georgfuchsia, Methyloversatilis, Sulfurisoma, and Exiguobacterium were the main microbial community in the S2--AuGS. This study proposed to form a novel S2--AuGS for developing the high ClO4- concentration removal performance and to utilize the S2- as an electron donor for controlling the excessive SO42- generation.

New insights of simultaneous partial nitritation, anammox and denitrification (SNAD) system to Zn(II) exposure: Focus on affecting the regulation of quorum sensing on extracellular electron transfer and microbial metabolism.

Here, the toxicity responses mechanism of the simultaneous partial nitritation, anammox and denitrification (SNAD) system to Zn(II) exposure were explored with emphasis on the repressed quorum sensing (QS) regulation on extracellular electron transfer and microbial metabolism. Results showed that Zn(II) accumulated in cells and induced oxidative stress, which led to microbial structure destruction. The increased electron transfer impedance and reduced redox substances (flavin/Cytochrome c) implied that Zn(II) affected electron transfer. The decreased ATP level, dehydrogenase and nitrogen related enzymatic activities showed Zn(II) affected organic matter and nitrogen metabolism. Furthermore, combined with Pearson network analysis, Zn(II) exposure disturbed the QS to decrease Acyl Homoserine Lactones (AHLs) secretion responsible for regulating extracellular electron transfer and microbial metabolism, thereby disturbing the performance of the SNAD system. This study provided new insights into the toxicity responses mechanism of the SNAD system to HM exposure.

Perturbation of clopyralid on bio-denitrification and nitrite accumulation: Long-term performance and biological mechanism.

The contaminant of herbicide clopyralid (3,6-dichloro-2- pyridine-carboxylic acid, CLP) poses a potential threat to the ecological system. However, there is a general lack of research devoted to the perturbation of CLP to the bio-denitrification process, and its biological response mechanism remains unclear. Herein, long-term exposure to CLP was systematically investigated to explore its influences on denitrification performance and dynamic microbial responses. Results showed that low-concentration of CLP (<15 mg/L) caused severe nitrite accumulation initially, while higher concentrations (35-60 mg/L) of CLP had no further effect after long-term acclimation. The mechanistic study demonstrated that CLP reduced nitrite reductase (NIR) activity and inhibited metabolic activity (carbon metabolism and nitrogen metabolism) by causing oxidative stress and membrane damage, resulting in nitrite accumulation. However, after more than 80 days of acclimation, almost no nitrite accumulation was found at 60 mg/L CLP. It was proposed that the secretion of extracellular polymeric substances (EPS) increased from 75.03 mg/g VSS at 15 mg/L CLP to 109.97 mg/g VSS at 60 mg/L CLP, which strengthened the protection of microbial cells and improved NIR activity and metabolic activities. Additionally, the biodiversity and richness of the microbial community experienced a U-shaped process. The relative abundance of denitrification- and carbon metabolism-associated microorganisms decreased initially and then recovered with the enrichment of microorganisms related to the secretion of EPS and N-acyl-homoserine lactones (AHLs). These microorganisms protected microbe from toxic substances and regulated their interactions among inter- and intra-species. This study revealed the biological response mechanism of denitrification after successive exposure to CLP and provided proper guidance for analyzing and treating herbicide-containing wastewater.

The effect of step-feeding distribution ratio on high concentration perchlorate removal performance in ABR system with heterotrophic combined sulfur autotrophic process.

In a lab-scale anaerobic baffled reactor (ABR) with eight compartments, the heterotrophic and sulfur autotrophic processes were combined to remove perchlorate. And then, the step-feeding distribution ratio of the heterotrophic perchlorate reduction unit (HPR unit) was optimized to achieve efficient removal of high concentration perchlorate. Under the optimized step-feeding distribution ratio, the perchlorate removal efficiency reached to 99.8% with the influent concentration of 1300 mg/L, indicating that the removal performance of step-feeding was better than that of normal-feeding. A mass balance results showed that the perchlorate removal capacity of the C1-C5 compartments were 11.8 ± 0.6, 13.2 ± 0.2, 11.7 ± 1.0, 8.8 ± 0.2 and 9.8 ± 1.0 g/d during the stage VIII, indicating that the step-feeding can effectively relieve pollutant loading of C1 compartment and improve the perchlorate removal capacity of the C2-C5 compartments. Moreover, the high-throughput sequencing analysis showed that bacterial community was significant difference between the HPR and sulfur autotrophic perchlorate removal (SAPR) units. Principal component analysis (PCA) showed that perchlorate removal was more positive correlation with the forward compartments than the posterior compartments of HPR unit. The study confirms that the optimized step-feeding ratio is beneficial to remove the high concentration perchlorate via combining heterotrophic and sulfur autotrophic processes.

Acceleration mechanism of bioavailable Fe(â ¢) on Te(IV) bioreduction of Shewanella oneidensis MR-1: Promotion of electron generation, electron transfer and energy level.

The release of highly toxic tellurite into the aquatic environment poses significant environmental risks. The acceleration mechanism and tellurium nanorods (TeNPs) characteristics with bioavailable ferric citrate (Fe(III)) were investigated in the tellurite (Te(IV)) bioreduction. Experiments showed that 5 mM Fe(III) increased the Te(IV) bioreduction rate from 0 to 12.40 mg/(L·h). Cyclic voltammetry, electrochemical impedance spectroscopy and Tafel were used to investigate electron transfer during Te(IV) bioreduction. NADH production (electron production) was significantly enhanced to 138% by Fe(III). Meanwhile Fe(III) stimulated the increase of cytochrome c, resulting in increased electron transport system activity. In addition, Fe(III) facilitated the secretion of extracellular polymeric substances (EPS) and reduced cell membrane permeability, thus reducing the toxicity of Te(IV) to cells. The increase of ATP provided energy for the metabolic process of Te(IV) bioreduction, playing an active role in cell activity. Based on the above analysis, the acceleration mechanism of Fe(III) on Te(IV) bioreduction was proposed from the aspects of electron generation, electron transfer and energy level. Zeta potential and FT-IR spectra indicated that the stability of TeNPs contributed to the covered EPS. This study provides further understanding the acceleration mechanism of Te(IV) bioreduction and promising strategy for improving the stability of TeNPs.

Feasibility of partial denitrification and anammox for removing nitrate and ammonia simultaneously in situ through synergetic interactions.

In this study, the single-stage partial denitrification-anammox (PD-A) process was started-up in 22 days in a lab-scale up-flow sludge blanket (UASB) reactor to treat wastewater containing NH4+-N and NO3--N simultaneously. The TN removal rate reached 97.08% with a low effluent TN of 10 mg/L. High-throughput sequencing results revealed the dominant bacterial strains were related to the genus of Thauera and Candidatus Kuenenia. The PD-A system was started-up based on the optimized PD process via inoculated exogenous anammox sludge attributing to the improvement of bacterial adaptation and co-existence by EPS. The PD process was realized in 18 days with the abundance of PD functional bacterium Thauera through fluctuated C/NO-3-N conditions. Moreover, the detrimental effects of starvation on anammox was weaker than that on PD bacteria. The PD-A process was expected to open a new possible perspective in designing NO3--N and NH4+-N wastewater treatment plants.

Effect and mechanism of cyclodextrins on nitrate reduction and bio-activity by S.oneidensis.MR-1.

Cyclodextrins (CDs) have been widely used due to the excellent solubilization of hydrophobic organics. However, their effect on the biotransformation process of hydrophilic pollutants is unclear. This study first evaluated the effect and mechanism of CDs on nitrate reduction by S.oneidensis.MR-1. The three CDs (α-CD, ß-CD and γ-CD) all accelerated nitrate reduction, among which ß-CD had the best effect. The nitrate reduction rate was increased by 21.8% with 0.5 mM ß-CD. As for the mechanism, ß-CD increased the biomass, membrane permeability and EPS of S.oneidensis.MR-1. The nitrate reductase activity was also increased by 1.34-fold with 0.5 mM ß-CD. The current exchange density and the electron transfer system activity were increased by 11.4% and 99.5% in the ß-CD-supply system, respectively. It confirmed that ß-CD enhanced the biological and electrochemical characteristics and then enhanced bio-activity. This study provides a new understanding of CDs in microbial remediation and broadens the practical application.

Landfill leachate as an additional substance in the Johannesburg-Sulfur autotrophic denitrification system in the treatment of municipal wastewater with low strength and low COD/TN ratio.

Johannesburg-Sulfur autotrophic denitrification (JHB-SAD) system was investigated for the combined treatment of leachate and municipal wastewater with low strength and low COD/TN ratio. The average removal efficiencies for chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP) were 85.2%, 96.2% and 75.8%, respectively. The municipal wastewater and leachate (dosing of 2.1‰, v/v) can be treated via the JHB-SAD system to achieve efficient nutrients removal. The mass balance calculations suggested that 58.1-69.8% TN was removed in JHB unit and 32.9-41.2% TN in SAD unit. Further, the denitrifying phosphorus removal process occurred in the anoxic zone. EEM-PARAFAC analysis found that the protein-like materials were more efficiently removed than fulvic-like materials in JHB-SAD system. The tryptophan-like materials had the most positive linear relationship with the COD concentrations. The bacterial community was difference between JHB and SAD unit. Furthermore, bacteria abundance relating to nitrogen removal increased with additional leachate.