ABSTRACT
Localized induction of DNA damage is a valuable tool for studying cellular DNA damage responses. In recent decades, methods have been developed to generate DNA damage using radiation of various types, including photons and charged particles. Here we describe a simple ultrasoft X-ray multi-microbeam system for high dose-rate, localized induction of DNA strand breaks in cells at spatially and geometrically adjustable sites. Our system can be combined with fixed- and live-cell microscopy to study responses of cells to DNA damage.
Subject(s)
DNA Damage/radiation effects , DNA Repair , DNA/radiation effects , X-Rays , Cell Line, Tumor , Green Fluorescent Proteins , Humans , Immunohistochemistry/methods , Microscopy, Fluorescence/methodsABSTRACT
The interest in terahertz photometric and imaging measurements has motivated the development of bandpass resonant filters to be coupled to multiple-pixel devices such as bolometer arrays. Resonant grids are relatively simple to fabricate, exhibiting high transmission at the central frequency, a narrow bandpass, and good rejection of the side frequencies of the spectrum. We have fabricated filters centered at different frequencies between 0.4 and 10 THz, using photolithography and electroforming techniques. Transmission measurements have shown center frequencies and bandwidths close to the design predictions. The performance of the filters was found not to be critically dependent on small physical deformations in the mesh, becoming more noticeable at higher frequencies (i.e., for smaller physical sizes). Wider bandwidths, needed to attain higher sensitivities in the continuum, were obtained by changing the design parameters for filters at 2 and 3 THz.