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A mechanism for substrate-Induced formation of 6-hydroxyflavin mononucleotide catalyzed by C30A trimethylamine dehydrogenase.
Lu, Xingliang; Nikolic, Dejan; Mitchell, Deanna J; van Breemen, Richard B; Mersfelder, John A; Hille, Russ; Silverman, Richard B.
Bioorg Med Chem Lett ; 13(22): 4129-32, 2003 Nov 17.
Article in English | MEDLINE | ID: mdl-14592522

ABSTRACT

Experiments are described to determine the origin of the 6-hydroxyl group of 6-hydroxyFMN produced by the substrate-induced transformation of FMN in the C30A mutant of trimethylamine dehydrogenase. The conversion of FMN to 6-hydroxyFMN is carried out in the presence of H(2)(18)O and 18O(2), and the results clearly show that the 6-hydroxyl group is derived from molecular oxygen and not from water.


Subject(s)
Flavin Mononucleotide/analogs & derivatives , Flavin Mononucleotide/biosynthesis , Flavin Mononucleotide/metabolism , Oxidoreductases, N-Demethylating/metabolism , Amino Acid Substitution , Cloning, Molecular , Escherichia coli/enzymology , Escherichia coli/genetics , Methylophilus methylotrophus/enzymology , Mutagenesis, Site-Directed , Recombinant Proteins/metabolism , Substrate Specificity
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