ABSTRACT
Dermatitis artefacta is one of a spectrum of factitious diseases etiologically responsible for skin lesions denied by patients. These factors often make it difficult to identify the causative agents of the condition. Herein, we report a case of bullous dermatitis artefacta in a 12-year-old girl, for which a deodorant spray was suspected as the probable cause. Pathological examination revealed subepidermal blistering with full-thickness necrosis of the epidermis, suggesting a thermo- or cryo-induced injury. Psychological testing demonstrated her immaturity and dependence. In searching for the causative agent, we suspected a deodorant spray as a blister-inducing agent. We succeeded in reproducing a similar blister lesion on the volunteer's healthy skin using the same spray. Psychiatric involvement significantly complicates the treatment of factitious diseases, including dermatitis artefacta. Cooperation among dermatologists, psychiatrists and the patient's family members is required for ensuring a favorable prognosis.
Subject(s)
Deodorants/adverse effects , Dermatitis/diagnosis , Leg Dermatoses/diagnosis , Self-Injurious Behavior/diagnosis , Child , Dermatitis/etiology , Dermatitis/pathology , Dermatitis/psychology , Diagnosis, Differential , Female , Humans , Leg Dermatoses/chemically induced , Leg Dermatoses/pathology , Leg Dermatoses/psychology , Self-Injurious Behavior/chemically induced , Self-Injurious Behavior/pathology , Self-Injurious Behavior/psychologyABSTRACT
POU domain proteins are a family of critical regulators of development and differentiation due to their transcriptional activity in the nucleus. Skn1a, a member of the POU domain protein family, appears to be expressed predominantly in epidermal keratinocytes and is thought to play a critical role in keratinocyte differentiation and proliferation. In this study, we examined the mechanisms involved in the nuclear localization of Skn1a. We transiently expressed enhanced green fluorescent protein (EGFP) reporter constructs encoding EGFP fusions with Skn1a deletion and mutation proteins in normal human epidermal keratinocytes (NHEKs). The experiments clearly demonstrated that Skn1a contained a functional nuclear localization signal (NLS) domain, and that the smallest domain necessary for Skn1a nuclear transport was the GRKRKKR sequence located within amino acids 279285. Previous studies have shown that the phosphorylation of specific amino acids neighboring the NLS may regulate nuclear transport and that the amino acid residues threonine (Thr) and serine (Ser) have the potential to undergo phosphorylation. We examined whether the amino acids Thr286 and Ser287, which reside adjacent to the NLS at the carboxyterminal side, play a role in Skn1a nuclear localization. For this purpose, we generated three EGFPSkn1a mutation constructs, in which Thr286, Ser287, or both Thr286 and Ser287 residues were replaced with alanine, respectively. The results showed that the Thr286 and Ser287 residues were involved in the regulation of nuclear localization as well as epidermal differentiation. These results suggested that the epidermal differentiation signaling pathway, involving kinase and phosphatase activation, may regulate the NLS activity of Skn1a in keratinocytes. Collectively, these data contribute to understanding the mechanisms of nuclear translocation of POU domain proteins and epidermal differentiation.
Subject(s)
Cell Differentiation/genetics , Nuclear Localization Signals/genetics , Octamer Transcription Factors/genetics , POU Domain Factors/genetics , Active Transport, Cell Nucleus , Epidermal Cells , Epidermis/metabolism , Green Fluorescent Proteins , Humans , Keratinocytes/cytology , Keratinocytes/metabolism , Octamer Transcription Factors/metabolism , POU Domain Factors/metabolism , Promoter Regions, Genetic , Protein Structure, Tertiary , Signal Transduction/geneticsABSTRACT
Despite a number of studies on signal transduction in epidermal keratinocytes, very little is known about how signals move from the cytosol to the nucleus during the course of keratinocyte proliferation and differentiation. In this study, we first compared the expression patterns of the karyopherin alpha (KPNA) subtypes, and found that KPNA2, KPNA3, and KPNA4 were the major subtypes in both normal human epidermal keratinocytes (NHEKs) and normal human dermal fibroblasts (NHDFs). Stimulation with either transforming growth factor (TGF)-beta1 or IFN-gamma for 24 hours resulted in the downregulation of KPNA2 expression specifically in NHEK at both the mRNA and protein levels. Interestingly, IFN-gamma, but not TGF-beta1, specifically downregulated KPNA2 expression at the promoter level, suggesting differential regulation of KPNA2 expression by IFN-gamma and TGF-beta1. We then demonstrated that KPNA2 physically bound to IFN regulatory factor-1 (IRF-1), a transcription factor induced by IFN-gamma, and induced nuclear translocation of IRF-1 in NHEKs. We finally performed microarray and quantitative real-time PCR analysis for the mRNA expression pattern of NHEK with either overexpression or knockdown of KPNA2, and indicated KPNA2 involvement for various epidermal gene regulations such as involucrin. Our data suggest that KPNA2 may play an important role in the signal-transduction pathways that regulate epidermal proliferation and differentiation.