ABSTRACT
STUDY DESIGN: Prospective study comparing the somatometric measurements of young males with normal spinal curves and with adolescent idiopathic scoliosis (AIS) with respect to the severity of AIS. OBJECTIVE: To assess the somatometric measurements of young males with normal spinal curves and with AIS using the conscription data. SUMMARY OF BACKGROUND DATA: The progression of AIS is closely correlated with longitudinal growth during puberty. Although abnormal growth in female AIS patients has been well investigated, the growth of male AIS patients has not been well reported. METHODS: During Korean conscription, 409 adolescent males with a normal spinal curvature and 420 adolescent males with AIS were enrolled. Those with AIS were grouped according to the severity of scoliosis using Cobb angles, according to the guidelines issued by the Korean military directorate. Group somatometric measurements, such as body height, corrected body height (corrected using Bjure's equation), body weight, and body mass index (BMI) were compared. RESULTS: Uncorrected heights were insignificantly different (P=0.234), but corrected heights, body weights, and BMIs were all significantly different between the normal and all AIS groups (P<0.001) (in AIS, corrected height was greater and body weight and BMIs corrected or uncorrected for height were lower). Cobb angles were not related to corrected body height or BMI, but was related to weight. Body weight was significantly less in the severe AIS group (Cobb angle >40 degrees) than in the mild or moderate AIS group (P<0.042). CONCLUSIONS: In Korean male AIS patients, significantly different somatometric results were observed: namely, a greater corrected height and a lower body weight and BMIs corrected or uncorrected for height. Furthermore, body weight was significantly lower in the severe group than in the moderate group. This study shows that abnormal growth is observed in male AIS patients and that body weight is closely correlated with AIS severity.
Subject(s)
Body Height , Body Mass Index , Body Weight , Scoliosis/pathology , Adolescent , Female , Humans , Male , Prospective StudiesABSTRACT
3'-Azido-2',3'-dideoxyuridine (AZDU) is a nucleoside analog structurally similar to zidovudine (AZT) with proven activity against human immunodeficiency virus (HIV). The purpose of this study was to develop and validate a high-performance liquid chromatographic (HPLC) method to quantitatively determine AZDU and its novel prodrugs in rat plasma simultaneously. A reversed-phase gradient elution HPLC method was developed to quantitate AZDU and its prodrugs, N3-pivaloyloxymethyl-3'-azido-2',3'-dideoxyuridine (I), 5'-pivaloyloxymethyl-3'-azido-2',3'-dideoxyuridine (II), 5'-O-valinyl-3'-azido-2',3'-dideoxyuridine hydrochloride (III) and 5'-O-phenylalanyl-3'-azido-2',3'-dideoxyuridine hydrochloride (IV), in rat plasma. AZDU and its prodrugs were analyzed using an octadecyl silane column with a mobile phase consisting of 0.04 microM sodium acetate buffer, pH 5.0, and acetonitrile, running in a segmented gradient manner at a flow rate of 2 ml/min. Acetonitrile was increased from 10 to 50% during the first 8 min by 5% per min, followed by 10% per min until it reached 90% acetonitrile. 3'-Azido-2',3'-dideoxy-5-ethyluridine (CS-85) was used as an internal standard (25 microg/ml). Compounds were detected by UV absorption at 261 nm. Extraction recoveries for all compounds were greater than 80%. Retention times of AZDU, CS-85, prodrugs I, II, III and IV were 3.3, 5.2, 9.1, 8.8, 6.3 and 7.3 min, respectively. Calibration plots were linear over the range of 0.25-100 microg/ml for AZDU and prodrugs II, III, and IV and 0.5-100 microg/ml for prodrug I. The limit of quantitation was 0.25 microg/ml for prodrugs II, III and IV and 0.5 microg/ml for prodrug I. The intra- and inter-day variations were less than 10% and accuracies were greater than 90%. This method is rapid, sensitive and reproducible for the determination of AZDU and prodrugs in rat plasma.