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1.
J Sch Nurs ; 39(6): 506-516, 2023 Dec.
Article in English | MEDLINE | ID: mdl-34662202

ABSTRACT

Given that the obesity rate among school-age children is increasing, school nurses can play a vital role in managing obesity and encouraging healthy living in school settings. Obese children from low-income backgrounds are more vulnerable than other students and require more careful attention and intervention. This qualitative study aimed to explore and understand the barriers recognized by school nurses in managing obesity in low-income household children. A focus group interview was conducted with 17 school nurses working at an elementary school. Children, home, school, political and structural, and social areas were revealed as intricate factors in obesity management. This study can help understand school nurses' obstacles in managing obese children from low-income families and can help them prepare practical measures to overcome these obstacles.


Subject(s)
Nurses , Pediatric Obesity , School Nursing , Humans , Child , Pediatric Obesity/epidemiology , Students , Republic of Korea
2.
Environ Sci Technol ; 54(12): 7185-7193, 2020 06 16.
Article in English | MEDLINE | ID: mdl-32496782

ABSTRACT

Despite the implementation of intensive phosphorus reduction measures, periodic outbreaks of cyanobacterial blooms in large rivers remain a problem in Korea, raising the need for more effective solutions to reduce their occurrence. This study sought to evaluate whether phosphorus or nitrogen limitation is an effective approach to control cyanobacterial (Microcystis) blooms in river conditions that favor this non-nitrogen-fixing genus. These conditions include nutrient enrichment, high water temperature, and thermal stratification during summer. Mesocosm bioassays were conducted to investigate the limiting factors for cyanobacterial blooms in a river reach where severe Microcystis blooms occur annually. We evaluated the effect of five different nitrogen (3, 6, 9, 12, and 15 mg/L) and phosphorus (0.01, 0.02, 0.05, 0.1, and 0.2 mg/L) concentrations on algae growth. The results indicate that nitrogen treatments stimulated cyanobacteria (mostly Microcystis aeruginosa) more than phosphorus. Interestingly, phosphorus additions did not stimulate cyanobacteria, although it did stimulate Chlorophyceae and Bacillariophyceae. We conclude that phosphorus reduction might have suppressed the growth of Chlorophyceae and Bacillariophyceae more than that of cyanobacteria; therefore, nitrogen or at least both nitrogen and phosphorus control appears more effective than phosphorus reductions alone for reducing cyanobacteria in river conditions that are favorable for non-nitrogen-fixing genera.


Subject(s)
Microcystis , Eutrophication , Lakes , Nitrogen/analysis , Phosphorus/analysis , Republic of Korea , Rivers
3.
Nurs Health Sci ; 18(2): 262-9, 2016 Jun.
Article in English | MEDLINE | ID: mdl-26581785

ABSTRACT

This study determines the effect of simulation with team-based learning (TBL) on newborn nursing care. This randomized controlled trial included 74 nursing students from one university located in Seoul, South Korea. Participants were categorized into two groups according to educational modality: one group involved both simulation and TBL, and the other involved simulation alone. Learning attitudes, academic achievement, and simulation performance were examined to assess effectiveness. The mean difference in learning attitudes between the two groups was non-significant. Low academic achievement differed significantly between the two groups (t = 3.445, P = 0.002). There was no significant difference in mean scores for simulation performance between the two groups. In this study, simulation with TBL was effective in improving learning outcomes. In current nursing education, various learning methods are employed within complex nursing situations and require flexibility and problem-solving approaches.


Subject(s)
Learning , Nursing, Team/methods , Pediatric Nursing/methods , Simulation Training/standards , Education, Nursing, Baccalaureate/methods , Female , Humans , Male , Pediatric Nursing/standards , Seoul , Simulation Training/methods , Students, Nursing/psychology
4.
J Nanosci Nanotechnol ; 15(8): 5589-92, 2015 Aug.
Article in English | MEDLINE | ID: mdl-26369122

ABSTRACT

δ-Catenin induces dendritic morphogenesis in several cells and it was reported that deletion of C-terminal 207 amino acid of δ-catenin completely abolished the dendritic morphogenesis. However, exact domain responsible for inducing dendritic morphogenesis in C-terminus of δ-catenin was not mapped. Here, we report that expression of ΔC47 (lacking 47 amino acid of C-terminus: 1-1200), ΔC77 (lacking 77 amino acid of C-terminus: 1-1170) deletion mutants of δ-catenin induced the dendritic morphogenesis of HEK293T and NIH3T3 cells as full-length δ-catenin did. In agreement with previous report, ΔC207 deletion mutant did not show the dendritic morphogenesis of the cells. Interestingly, introducing 107 amino acid deletion of C-terminus (ΔC107 mutant: 1-1140) and 177 amino acid deletion of C-terminus (ΔC177 mutant: 1-1070) showed limited primary and secondary dendritic process and notable spine-like process formation. These results suggest that 1140-1170 amino acid of C-terminal δ-catenin is required for primary and secondary dendrite-like process formation.


Subject(s)
Dendrites/physiology , Dendrites/ultrastructure , Morphogenesis/physiology , alpha Catenin/chemistry , alpha Catenin/metabolism , Amino Acid Sequence , Animals , HEK293 Cells , Humans , Mice , Molecular Sequence Data , Mutagenesis, Site-Directed , NIH 3T3 Cells , Structure-Activity Relationship , alpha Catenin/genetics
5.
J Nurs Care Qual ; 30(4): E26-31, 2015.
Article in English | MEDLINE | ID: mdl-26018567

ABSTRACT

"QUality Of care Through the patients' Eyes" (QUOTE) instruments for measuring care quality were classified in terms of procedures, structures, and content. The content and structures of each instrument were found to include (a) participation of the client in the tool development process, (b) evaluation of the importance and performance of care based on the client's needs, and


Subject(s)
Psychometrics , Quality of Health Care , Surveys and Questionnaires , Humans , Needs Assessment , Patient Satisfaction , Patient-Centered Care
6.
J Nurs Educ ; 52(3): 150-6, 2013 Mar.
Article in English | MEDLINE | ID: mdl-23410252

ABSTRACT

The use of film in nursing and medical education has been supported as an effective instructional method. The purpose of this article is to identify and synthesize the available studies on teaching-learning strategies to be used with film for prelicensure students. Electronic databases were searched to identify studies published in the English language between January 1990 and March 2012. Twenty-seven articles met the selection criteria for this review and were analyzed. After in-depth discussion about and investigation of the relevant literature, we narrowed down three teaching-learning strategies: reflective activities, practical activities, and evaluative activities. The synthesis of the identified teaching-learning strategies provides a data point for the development of more effective evidence-based learning activities for prelicensure students. Future studies should focus on the examination of teaching effectiveness and learning outcomes, as well as the evaluation of using film, to achieve nursing competencies appropriate to role preparation.


Subject(s)
Education, Nursing/methods , Motion Pictures , Humans , Teaching/methods
7.
J Nurs Educ ; 52(7): 391-6, 2013 Jul.
Article in English | MEDLINE | ID: mdl-23755942

ABSTRACT

Although the literature has highlighted the use of virtual worlds in teaching-learning, little is known about the concepts associated with this technology in nursing education. Moreover, the application of virtual worlds to education has been underdeveloped theoretically, with much of the work being exploratory. Thus, the aim of this integrative review was to identify the current evidence on the use of virtual worlds in the education of nursing and other health professional students and to describe emerging themes surrounding this phenomenon. We searched seven electronic databases for relevant articles and used Whittemore's and Knafl's integrative review method to synthesize the literature. Twelve articles met the selection criteria for this review, from which three overarching themes emerged: (a) critical reasoning skills, (b) student-centered learning, and (c) instructional design considerations. This integrative review extends our understanding of virtual worlds in nursing education and the potential barriers and facilitators of their use.


Subject(s)
Education, Nursing/methods , Educational Technology , Social Networking , Teaching/methods , User-Computer Interface , Video Games , Humans
8.
Cancers (Basel) ; 15(24)2023 Dec 07.
Article in English | MEDLINE | ID: mdl-38136291

ABSTRACT

PURPOSE: Antibiotic use preceding immune checkpoint inhibitor (ICI) treatment has been associated with a decreased efficacy of ICI in solid tumors. In this study, we evaluated the effect of antibiotic use before ICI therapy on oncological outcomes. METHODS: We examined patients with recurrent gynecologic malignancies at two academic institutions. The clinical data, including antibiotic use within 60 days of ICI initiation, type of antibiotics, reasons for antibiotic use, body mass index, tumor site, chemotherapy-free interval, prior history of radiotherapy, disease control rate (DCR), and overall survival (OS), were assessed. RESULTS: Of 215 patients, 22.9% (n = 47) received antibiotics before ICI treatment. The most common cancer was ovarian (52.1%, n = 112), followed by cervical (24.7%, n = 53) and endometrial (16.7%, n = 36). When we divided the cohort based on antibiotic use before ICIs, there were no significant differences in the DCR and baseline characteristics between the two groups. On multivariate analyses, the variables associated with poor OS were previous use of antibiotics for a cumulative duration of >14 days (HR 2.286, 95% CI 1.210-4.318; p = 0.011); Eastern Cooperative Oncology Group 2 or 3 (HR 4.677, 95% CI 2.497-8.762; p < 0.001); and chemotherapy-free interval of <6 months (HR 2.007, 95% CI 1.055-3.819; p = 0.034). CONCLUSION: Prior use of antibiotics for a cumulative duration of >14 days was associated with reduced survival in recurrent gynecologic malignancies.

9.
Article in English | MEDLINE | ID: mdl-35206165

ABSTRACT

The purpose of this study was to develop and evaluate the effectiveness of the course on Global Health Nursing for Indian nursing students, intended to introduce the course as a selective course in the nursing curriculum. METHODS: A quasi-experimental nonequivalent control group pre- and post-test design was carried out. The study participants were fifty final grade nursing students, considered as an experimental (n = 25) and control group (n = 25). The participants recruited by purposive sampling were fifty 4th grade nursing students (experimental 25, control 25) at St. X College of Nursing, India. The course on Global Health Nursing was developed by using the ADDIE model, and the duration of the course was about 16 h over three weeks. The course was implemented based on Gagné's instructional method. Data were collected by self-perceived scales of Global Health Nursing before and after the course, the participants' opinions, and feedback providing comments about the course. RESULTS: The study results showed improvement in knowledge (F = 8.48; p < 0.001), skills (F = 96.14; p < 0.001), and performance on Global Health Nursing (F = 5.50; p < 0.001) and was statistically significant. Moreover, the participants described that they were satisfied with the quality of teaching-learning and achieved the learning goals. CONCLUSION: The outcome of this study could be extended to the existing nursing curriculum and would be modified to formulate a standard module in the curriculum of the Indian nursing system. The study offers implications for different fields of nursing, including nursing education, research, and practice in India.


Subject(s)
Education, Nursing, Baccalaureate , Education, Nursing , Students, Nursing , Curriculum , Global Health , Humans , Learning
10.
Healthcare (Basel) ; 10(2)2022 Feb 01.
Article in English | MEDLINE | ID: mdl-35206902

ABSTRACT

This study attempted to examine and compare the job-seeking anxiety and job preparation behavior of undergraduate students. A descriptive cross-sectional study was employed; the study participants were 360 students (3rd and 4th grade), selected from K' university in G city. Data were collected by structured self-reported questionnaires from November 2020 to February 2021. Variables included general characteristics, job-seeking anxiety and job preparation behavior and were analyzed by descriptive statistics, Pearson's correlation, and multiple regression analysis by using the SPSS/WIN 25.0 program. Of the total population, 70.8% were female in the health and social science group, the age group was 22-24 years (55.2%; 50.2%) and were fourth grade 62%; 59.1%). The level of job-seeking anxiety of students showed a higher proportion in health science (4.45 ± 0.81) than social science (3.73 ± 0.55). The level of job preparation behavior also revealed the same results in health science (4.28 ± 0.76) and social science (4.06 ± 0.81). Job anxiety showed a positive correlation with employment anxiety induction situation (r = 0.32, p < 0.01) and employment anxiety induction causes (r = 0.27, p < 0.01), and social science students showed a positive correlation with employment anxiety induction situation (r = 0.24, p < 0.01) and employment anxiety induction causes (r = 0.23, p < 0.01). The factors of age, gender and desired job position are highly associated with job-seeking anxiety and job preparation behavior. The findings of this study revealed job-seeking anxiety was higher among the undergraduate students and showed a high level of job preparation behavior. There is a need to develop intervention strategies for promoting job preparation behavior and reducing job-seeking anxiety among undergraduate students by providing career planning to improve the positive attitude towards desired job selection.

11.
Article in English | MEDLINE | ID: mdl-35055710

ABSTRACT

Cultural competence is a crucial requirement of nursing to promote caring for patients with diverse backgrounds. The purpose of this study was to develop a cultural competence course and to evaluate the effects of the course on undergraduate nursing students in Vietnam. A concurrent triangulation mixed-methods study was adopted using quantitative and qualitative data sources. Sixty-six nursing students were recruited for the following groups: cultural competence course with field experience (n = 22), stand-alone cultural competence course (n = 22), and a control group (n = 22). The findings indicated that significant group by time interactions in total cultural competence score (F = 66.73, p < 0.001) were found. Participants' perceptions reflected on three categories: (a) journey to cultural competence, (b) satisfaction of cultural competence course, and (c) suggestions for improvements. No statistically significant differences between the two experimental groups were revealed, but "obtaining cultural experiences" and "expanding understanding of cultural competence through field experience" were immersed from participants having field experience. It is vital to expand cultural competency education into nursing curricula to enhance nursing students' perspective of culturally competent care.


Subject(s)
Education, Nursing, Baccalaureate , Students, Nursing , Cultural Competency/education , Culturally Competent Care , Curriculum , Humans , Vietnam
12.
Pest Manag Sci ; 78(12): 5402-5412, 2022 Dec.
Article in English | MEDLINE | ID: mdl-36057130

ABSTRACT

BACKGROUND: Recently, cyantraniliprole (CYA) and sulfoxaflor (SUL) have been considered as alternatives to neonicotinoid insecticides. In this study, we evaluated the acute and chronic toxicities of CYA and SUL on honey bee (Apis mellifera L.) larvae reared in vitro. RESULTS: In the acute toxicity test, the following test doses were used to determine the median lethal dose (LD50 ): CYA 0.007, 0.014, 0.028, 0.056 and 0.112 µg larva-1 ; SUL 2.5, 5, 10, 20 and 40 µg larva-1 . In the chronic toxicity test, the following test doses were used to determine the LD50 : CYA 0.00512, 0.0128, 0.032, 0.08 and 0.2 µg larva-1 ; SUL 0.0625, 0.125, 0.25, 0.5 and 1.0 µg larva-1 . The acute LD50 values of CYA and SUL were 0.047 and 11.404 µg larva-1 , respectively. Larvae acutely exposed to SUL had significantly lower body weight than controls, but those exposed to CYA showed no difference. The no observed adverse effect level (NOAEL) and LD50 values of the chronic toxicity tests for each insecticide were 0.00512 and 0.064 µg larva-1 for CYA, and 0.0625 µg larva-1 and 0.212 µg larva-1 for SUL, respectively. Larvae chronically exposed to SUL emerged as bees with deformed wings, reaching adult deformation rates of over 50%; however, CYA had no effect on adult deformation. CONCLUSION: Exposure to CYA increased larval mortality but did not cause any adult deformation, whereas SUL exposure increased pupal mortality and caused wing deformation in newly emerged bees. Our study may be useful for the assessment of pesticide toxicity by providing valuable findings on the effects of these insecticides on honey bee larvae. © 2022 Society of Chemical Industry.


Subject(s)
Insecticides , Bees , Animals , Larva , Insecticides/pharmacology , ortho-Aminobenzoates/pharmacology
13.
Toxics ; 10(7)2022 Jul 04.
Article in English | MEDLINE | ID: mdl-35878274

ABSTRACT

Thiram, an oxidized dimer of dithiocarbamate, has fungicidal and ectoparasiticidal roles. This study aimed to determine the effects of thiram on the development of zebrafish (ZF) embryos. The developmental toxicity test was performed in accordance with the OECD 236 test guidelines, and ZF embryos were subjected to several thiram concentrations and a DMSO (0.01%) control. Subsequently, embryo mortalities and developmental anomalies were evaluated at different hours post fertilization (hpf). Thiram was highly toxic to ZF, with calculated median lethal concentrations (LC50) of thiram at 48 and 96 h as 13.10 ± 2.17 and 8.87 ± 2.09 µg/L, respectively. Thiram-treated embryos/larvae exhibited a variety of deformities, such as abnormal somites, reduced eye pigment, abnormal tail shape, yolk sac edema, hatching defects, and curved spines, with a median effective concentration (EC50) of 3.88 ± 1.23, 5.04 ± 1.82, 6.23 ± 0.92, 5.24 ± 2.22, 1.39 ± 0.25, and 2.60 ± 0.82 µg/L, respectively. Teratogenic index (TI) values ranged from 1.42 to 6.66 for the scored deformities. At 48 hpf, the average heartbeat of the control group was 177.20 ± 5.63 per minute, while the highest thiram-treated group (40 µg/L) was 99.50 ± 18.12 per minute. In addition, cardiac-related issues, such as pericardial edema and abnormal blood flow, were observed in thiram-treated ZF embryos. Overall, these findings suggest that thiram is teratogenic to ZF.

14.
Asian Nurs Res (Korean Soc Nurs Sci) ; 15(5): 317-326, 2021 Dec.
Article in English | MEDLINE | ID: mdl-34748998

ABSTRACT

PURPOSE: Many studies have maintained that nursing support is necessary and essential for mothers of preterm infants; however, the perceived nursing support for mothers of preterm infants has not been sufficiently measured. This study aimed to develop a perceived nursing support scale for mothers of preterm infants (PNSS-MP). METHODS: The preliminary items of the PNSS-MP were developed through a literature review and in-depth interviews with mothers of preterm infants. Content and face validities were assessed by experts and mothers of preterm infants. A pilot study was conducted to confirm the feasibility and comprehension of the scale. To validate the PNSS-MP, 223 mothers of preterm infants were surveyed. Exploratory factor analyses were performed to confirm construct validity. Convergent and discriminant validities were analyzed using a multitrait-multimethod (MTMM) matrix. Reliability was tested by calculating Cronbach's α and performing split-half testing. RESULTS: The PNSS-MP consisted of 27 items and was categorized into five factors, explaining 65.3% of the total variance. The factors were named: "baby care support" (7 items), "mental care support" (6 items), "maternal role support" (6 items), "introducing resources support" (4 items), and "information delivery support" (4 items). The overall reliability of the scale was .95. CONCLUSION: The PNSS-MP adequately reflected the neonatal intensive care unit (NICU) in South Korea. Additionally, the PNSS-MP proved relatively valid and reliable; therefore, it can be used to measure nursing support in the NICU.


Subject(s)
Infant, Premature , Mothers , Female , Humans , Infant , Infant, Newborn , Intensive Care Units, Neonatal , Pilot Projects , Reproducibility of Results
15.
Child Health Nurs Res ; 27(2): 146-159, 2021 Apr.
Article in English | MEDLINE | ID: mdl-35004505

ABSTRACT

PURPOSE: The aim of this study was to clarify and define the concept of nursing support as perceived by mothers of preterm infants. METHODS: The research was conducted using Schwartz-Barcott and Kim's hybrid model. Sixteen articles were analyzed during the theoretical phase. In-depth interviews were conducted with 10 mothers of preterm infants in the fieldwork phase. In the final analytical phase, the attributes of nursing support were defined. RESULTS: There were four themes and 10 attributes of nursing support as perceived by mothers of preterm infants. Professional care and emotional care for the baby were identified as attributes of baby care support. Information related to the disease, inpatient environment, baby's daily hospital life, and mother-centered care were identified as attributes of information delivery support. Empathy for mothers and therapeutic communication with the mother were identified as attributes for mental care support. Lastly, providing a chance for the mother to take care of the baby and reinforcement of the maternal role were identified as attributes of maternal role support. CONCLUSION: The 10 attributes of this study could provide a base for the development of a nursing support instrument and practical guidelines for mothers in neonatal intensive care units.

16.
Article in English | MEDLINE | ID: mdl-33530442

ABSTRACT

BACKGROUND: The Nursing Profession Self-Efficacy (NPSE) scale was developed to reflect the characteristics of nursing tasks. This study was conducted to validate the Korean version of the NPSE (K-NPSE) scale. METHODS: The NPSE scale with nineteen items was translated into Korean after forward and backward translation according to Devellis' guideline. For the exploratory factor analysis (EFA), 298 nurses participated and criterion-related validity and reliability were verified. For the confirmatory factor analysis (CFA), 218 other nurses participated. Content validity, criterion-related validity, and internal consistency reliability were examined. Additionally, construct validity was examined. SPSS and AMOS were used for the data analyses. RESULTS: Nineteen items were selected after evaluating the content and cognitive validity and comprised three factors: "Professional (10 items)", "Advocating (4)", and "Caring (5)". Construct validity was supported by the CFA. Criterion-related validity was supported by comparison with the General Self-Efficacy Test (r = 0.43, p < 0.001). Cronbach's alpha of the K-NPSE was 0.93. CONCLUSIONS: Study findings indicate that the K-NPSE could be useful for assessing nurses' self-efficacy. The K-NPSE may be used as a valuable reference for developing programs or policies that promote nursing professionals. It is expected that continued use of this scale in various clinical settings to further generalize and validate the scale.


Subject(s)
Self Efficacy , Translating , Humans , Psychometrics , Reproducibility of Results , Republic of Korea , Surveys and Questionnaires
17.
Article in English | MEDLINE | ID: mdl-34360090

ABSTRACT

This study develops a scale that assesses the self-efficacy of Sri Lankan nursing educators in assuming the roles of nursing educators and validates its psychometric properties. This methodological research followed the DeVellis Scale Development Model, which involves six steps of instrument development and evaluation. Preliminary items were determined through a literature review and focus group interviews with nine Sri Lankan nursing experts. The experts, comprising five South Korean and two Sri Lankan nursing professors, tested the scale's content validity. Moreover, 15 nursing educators participated in a pilot study, and 126 educators took part in the main survey. To evaluate the scale's validity and reliability, the data from a preliminary questionnaire were analyzed using SPSS/IBM and AMOS 24.0. Further, construct validity was tested using exploratory and confirmatory factor analyses, and reliability was tested by calculating Cronbach's alpha and performing split-half testing. Finally, 39 items under four themes, "clinical mentorship" (18 items), "research" (10), "teaching" (6), and "advising" (5), explained 63.5% of the total variance. Confirmatory factor analysis results revealed an acceptable model fit for the final scale. The developed scale achieved a Cronbach's alpha value of 0.97. Thus, the psychometrical properties of the scale measuring Sri Lankan nursing educators' self-efficacy were comprehensively evaluated and found acceptable. The developed scale will be useful in guideline development or studies regarding the self-efficacy of nursing educators' roles in developing countries with similar context to Sri Lanka.


Subject(s)
Self Efficacy , Humans , Pilot Projects , Psychometrics , Reproducibility of Results , Sri Lanka , Surveys and Questionnaires
18.
Genes (Basel) ; 12(11)2021 10 29.
Article in English | MEDLINE | ID: mdl-34828343

ABSTRACT

Organophosphate pesticides (OPPs) are one of the most widely used insecticides. OPPs exert their neurotoxic effects by inhibiting acetylcholine esterase (AChE). Most of the gross developmental abnormalities observed in OPP-treated fish, on the other hand, may not be explained solely by AChE inhibition. To understand the overall molecular mechanisms involved in OPP toxicity, we used the zebrafish (ZF) model. We exposed ZF embryos to an OPP, phosmet, for 96 h, and then analyzed developmental abnormalities and performed whole transcriptome analysis. Phenotypic abnormalities, such as bradycardia, spine curvature, and growth retardation, were observed in phosmet-treated ZF (PTZF). Whole transcriptome analysis revealed 2190 differentially expressed genes (DEGs), with 822 and 1368 significantly up-and downregulated genes, respectively. System process and sensory and visual perception were among the top biological pathways affected by phosmet toxicity. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed significant enrichment of metabolic pathways, calcium signaling pathway, regulation of actin cytoskeleton, cardiac muscle contraction, drug metabolism-other enzymes, and phototransduction. Quantitative real-time PCR results of six DEGs agreed with the sequencing data expression profile trend. Our findings provide insights into the consequences of phosmet exposure in ZF, as well as an estimate of the potential risk of OPPs to off-target species.


Subject(s)
Embryo, Nonmammalian/drug effects , Embryo, Nonmammalian/metabolism , Phosmet/toxicity , Transcriptome , Zebrafish/genetics , Zebrafish/metabolism , Actin Cytoskeleton/drug effects , Animals , Gene Expression Profiling/methods , Gene Expression Regulation, Enzymologic , Insecticides/toxicity , Metabolic Networks and Pathways/drug effects , Myocardium , Organophosphorus Compounds/toxicity , Signal Transduction/drug effects , Water Pollutants, Chemical/toxicity
19.
PLoS One ; 16(8): e0255110, 2021.
Article in English | MEDLINE | ID: mdl-34339426

ABSTRACT

The present study aims to apply a DNA barcoding tool through amplifying two mitochondrial candidate genes i.e., COI and 16S rRNA for accurate identification of fish, aquatic molluscs and crustaceans of Sundarbans mangrove wetland, to build a reference library of fish and shellfishes of this unique ecosystems. A total of 185 mitochondrial COI barcode sequences and 59 partial sequences of the 16S rRNA gene were obtained from 120 genera, 65 families and 21 orders of fish, crustaceans and molluscs. The collected samples were first identified by examining morphometric characteristics and then assessed by DNA barcoding. The COI and 16S rRNA sequences of fishes and crustaceans were clearly discriminated among genera in their phylogenies. The average Kimura two-parameter (K2P) distances of COI barcode sequences within species, genera, and families of fishes are 1.57±0.06%, 15.16±0.23%, and 17.79±0.02%, respectively, and for 16S rRNA sequences, these values are 1.74±.8%, 0.97±.8%, and 4.29±1.3%, respectively. The minimum and maximum K2P distance based divergences in COI sequences of fishes are 0.19% and 36.27%, respectively. In crustaceans, the K2P distances within genera, families, and orders are 1.4±0.03%, 17.73±0.15%, and 22.81±0.02%, respectively and the minimum and maximum divergences are 0.2% and 33.93%, respectively. Additionally, the present study resolves the misidentification of the mud crab species of the Sundarbans as Scylla olivacea which was previously stated as Scylla serrata. In case of molluscs, values of interspecific divergence ranges from 17.43% to 66.3% in the barcoded species. The present study describes the development of a molecular and morphometric cross-referenced inventory of fish and shellfish of the Sundarbans. This inventory will be useful in future biodiversity studies and in forming future conservation plan.


Subject(s)
Avicennia , DNA Barcoding, Taxonomic , Ecosystem , Fishes/classification , Fishes/genetics , Seawater , Shellfish/classification , Animals , Base Sequence , Electron Transport Complex IV/genetics , Genetic Variation , Geography , Mollusca/genetics , Phylogeny , RNA, Ribosomal, 16S/genetics
20.
Insects ; 12(7)2021 Jul 01.
Article in English | MEDLINE | ID: mdl-34357263

ABSTRACT

Rearing honey bee larvae in vitro is an ideal method to study honey bee larval diseases or the toxicity of pesticides on honey bee larvae under standardized conditions. However, recent studies reported that a horizontal position may cause the deformation of emerged bees. Accordingly, the purpose of this study was to evaluate the emergence and deformation rates of honey bee (Apis mellifera ligustica) larvae reared in horizontal and vertical positions. The study was conducted under the same laboratory conditions with three experimental groups, non-capped or capped horizontal plates and capped vertical plates. However, our results demonstrated that the exhibited adult deformation rates of the horizontal plates were significantly higher (27.8% and 26.1%) than those of the vertical plates (11.9%). In particular, the most common symptoms were deformed wings and an abnormal abdomen in the horizontal plates. Additionally, adults reared on horizontal plates were substantially smaller (10.88 and 10.82 mm) than those on vertical plates (11.55 mm). Considering these conclusions, we suggest that a vertical rearing method is more suitable when considering the deformation rates of the control groups to verify the sublethal effects of pesticides on honey bees.

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