A diRNA-protein scaffold module mediates SMC5/6 recruitment in plant DNA repair.

In eukaryotes, the STRUCTURAL MAINTENANCE OF CHROMOSOME 5/6 (SMC5/6) complex is critical to maintaining chromosomal structures around double-strand breaks (DSBs) in DNA damage repair. However, the recruitment mechanism of this conserved complex at DSBs remains unclear. In this study, using Arabidopsis thaliana as a model, we found that SMC5/6 localization at DSBs is dependent on the protein scaffold containing INVOLVED IN DE NOVO 2 (IDN2), CELL DIVISION CYCLE 5 (CDC5), and ALTERATION/DEFICIENCY IN ACTIVATION 2B (ADA2b), whose recruitment is further mediated by DNA-damage-induced RNAs (diRNAs) generated from DNA regions around DSBs. The physical interactions of protein components including SMC5-ADA2b, ADA2b-CDC5, and CDC5-IDN2 result in formation of the protein scaffold. Further analysis indicated that the DSB localization of IDN2 requires its RNA-binding activity and ARGONAUTE 2 (AGO2), indicating a role for the AGO2-diRNA complex in this process. Given that most of the components in the scaffold are conserved, the mechanism presented here, which connects SMC5/6 recruitment and small RNAs, will improve our understanding of DNA repair mechanisms in eukaryotes.

Transport and distribution of residual nitrogen in ion-adsorption rare earth tailings.

A large amount of nitrogen remains in ion-absorption rare earth tailings with in-situ leaching technology, and it continually ends up in groundwater sources. However, the distribution and transport of ammonium nitrogen (NH4+-N) and nitrate nitrogen (NO3--N) across tailings with both depth and hill slopes is still unknown. In this study, the amount of NH4+-N and nitrate nitrogen (NO3--N) was determined in tailings, and a soil column leaching experiment, served to assess the transport and distribution following mine closure. Firstly, a high concentration of NH4+-N in the leachate at the initial leaching stage was detected, up to 2000 mg L-1, and the concentration of NH4+-N clearly diminished as time passed. Meanwhile, the NH4+-N contents remained relatively high in soil. Secondly, both the content of NH4+-N and NO3--N varied greatly according to vertical distribution after leaching lasting several years. The amounts of NH4+-N and NO3--N in surface soil were much smaller than those in deep soil, with 3-4 orders of magnitude variation with depth. Thirdly, when disturbed by NH4+-N, the pH not only diminished but also changed irregularly as depth increased. Fourthly, although the amount of NO3--N was smaller than that of NH4+-N, both their distribution trend was similar with depth. In fact, NH4+-N and NO3--N were significantly correlated but this declined from the knap to the piedmont. Based on these results, it is suggested that mining activity could cause nitrogen to be dominated by NH4+-N and acidification in a tailing even if leaching occurs over several years. NO3--N derived from NH4+-N transports easily and it becomes the main nitrogen pollutant with the potential to be a long-lasting threat to the environment around a mine.

Calculation of Toxicity Coefficient of Potential Ecological Risk Assessment of Rare Earth Elements.

Rare earth elements (REEs) are applied in various industries. They have entered the environment through different pathways and caused serious pollutions. So far, due to the lack of calculated toxicity coefficient of rare earth elements, it is still difficult to evaluate their ecological risks. The potential ecological risk index method is commonly used in the pollution assessment of heavy metals. And rare earth elements are similar to heavy metals. Herein, we used this method to calculate the toxicity coefficient of 15 rare earth elements (La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu, and Y). The calculation was based on two principles, rare earth elements coexist with each other because of their similar chemical properties, and the elemental abundance and release effect determine their toxicity. The results are as follows: La = 1, Ce = 1, Pr = 5, Nd = 2, Sm = 5, Eu = 10, Gd = 5, Tb = 10, Dy = 5, Ho = 10, Er = 5, Tm = 10, Yb = 5, Lu = 20, Y = 2. Our results can provide a reference to the potential ecological risk assessment of rare earth elements.

Redistribution and chemical speciation of rare earth elements in an ion-adsorption rare earth tailing, Southern China.

Mining is an activity that will change the distribution and chemical speciation of rare earth elements (REEs), thus posing a serious threat to the natural environment. However, the distribution and chemical speciation of REEs in ion-adsorption rare earth tailings remain poorly understood. In this study, we investigated the contents and forms of REEs and associated geochemical behavior in rare earth tailings in southeast China. Total rare earth elements (TREEs) contents were lower while the ratios of light REEs (LREEs) to heavy REEs (HREEs) were higher in tailings than in an unmined area. In the unmined area, the distribution characteristics of TREEs and LREEs remained consistent, whereas HREEs differed with increasing depth. However, in the tailing area, the distribution characteristics of TREEs, LREEs and HREEs tended to be consistent, reflecting the outcomes of mining activities on vertical distribution characteristics of REEs. The REEs were dominated by residual and exchangeable forms in the unmined area, while residual and exchangeable REEs accounted for 80% and 20% of the TREEs, respectively, in the three tailings. Additionally, the exchangeable and carbonate-bound REEs increased but Fe/Mn oxide-bound and organic-bound REEs declined in the unmined area, whereas their distribution characteristics were irregular in the tailings. These results suggest that mining activity could curtail REEs contents and redistribute their chemical speciation, further altering geochemical behaviors in the tailings and posing serious risks to adjacent environments.