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1.
Nat Rev Neurosci ; 17(5): 293-306, 2016 May.
Article in English | MEDLINE | ID: mdl-27052382

ABSTRACT

In an increasing number of states and countries, cannabis now stands poised to join alcohol and tobacco as a legal drug. Quantifying the relative adverse and beneficial effects of cannabis and its constituent cannabinoids should therefore be prioritized. Whereas newspaper headlines have focused on links between cannabis and psychosis, less attention has been paid to the much more common problem of cannabis addiction. Certain cognitive changes have also been attributed to cannabis use, although their causality and longevity are fiercely debated. Identifying why some individuals are more vulnerable than others to the adverse effects of cannabis is now of paramount importance to public health. Here, we review the current state of knowledge about such vulnerability factors, the variations in types of cannabis, and the relationship between these and cognition and addiction.


Subject(s)
Behavior, Addictive/chemically induced , Cannabis/adverse effects , Cognition/drug effects , Marijuana Smoking/adverse effects , Animals , Behavior, Addictive/metabolism , Behavior, Addictive/psychology , Brain/drug effects , Brain/metabolism , Cannabis/metabolism , Case-Control Studies , Cognition/physiology , Humans , Marijuana Smoking/metabolism , Marijuana Smoking/psychology , Receptor, Cannabinoid, CB1/metabolism , Risk Factors
2.
Nat Rev Neurosci ; 16(10): 579-94, 2015 Oct.
Article in English | MEDLINE | ID: mdl-26373473

ABSTRACT

Brain endocannabinoid (eCB) signalling influences the motivation for natural rewards (such as palatable food, sexual activity and social interaction) and modulates the rewarding effects of addictive drugs. Pathological forms of natural and drug-induced reward are associated with dysregulated eCB signalling that may derive from pre-existing genetic factors or from prolonged drug exposure. Impaired eCB signalling contributes to dysregulated synaptic plasticity, increased stress responsivity, negative emotional states and cravings that propel addiction. Understanding the contributions of eCB disruptions to behavioural and physiological traits provides insight into the eCB influence on addiction vulnerability.


Subject(s)
Endocannabinoids/physiology , Marijuana Abuse/physiopathology , Reward , Signal Transduction/physiology , Animals , Behavior, Addictive/psychology , Brain/physiology , Brain/physiopathology , Endocannabinoids/genetics , Humans , Neural Pathways/physiopathology , Signal Transduction/genetics
3.
Alcohol Clin Exp Res ; 44(11): 2158-2165, 2020 11.
Article in English | MEDLINE | ID: mdl-32944989

ABSTRACT

BACKGROUND: Ethanol (EtOH) self-administration is particularly sensitive to the modulation of CB1 signaling in the nucleus accumbens (NAc) shell, and EtOH consumption increases extracellular levels of the endogenous cannabinoid CB1 receptor agonist 2-arachidonoyl glycerol (2-AG) in this brain region. Stimulation of CB1 receptor with agonists increases EtOH consumption, suggesting that EtOH-induced increases in 2-AG might sustain motivation for EtOH intake. METHODS: In order to further explore this hypothesis, we analyzed the alterations in operant EtOH self-administration induced by intra-NAc shell infusions of 2-AG itself, the CB1 inverse agonist SR141716A, the 2-AG clearance inhibitor URB602, anandamide, and the cyclooxygenase-2 (COX-2) inhibitor nimesulide. RESULTS: Surprisingly, self-administration of 10% EtOH was dose-dependently reduced by either intra-NAc shell SR141716A or 2-AG infusions. Similar effects were found by intra-NAc shell infusions of URB602, suggesting again a role for accumbal 2-AG on the modulation of EtOH intake. Intra-NAc shell anandamide did not alter EtOH self-administration, pointing to a specific role for 2-AG in the modulation of EtOH self-administration. Finally, the inhibitory effect of intra-NAc shell 2-AG on EtOH intake was significantly reversed by pretreatment with nimesulide, suggesting that oxidative metabolites of 2-AG might mediate these inhibitory effects on operant self-administration. CONCLUSIONS: We propose that 2-AG signaling in the NAc exerts an inhibitory influence on EtOH consumption through a non-CB1 receptor mechanism involving the COX-2 pathway.


Subject(s)
Alcohol Drinking/drug therapy , Arachidonic Acids/pharmacology , Cyclooxygenase 2 Inhibitors/pharmacology , Endocannabinoids/pharmacology , Glycerides/pharmacology , Nucleus Accumbens/drug effects , Animals , Arachidonic Acids/antagonists & inhibitors , Biphenyl Compounds/pharmacology , Dose-Response Relationship, Drug , Endocannabinoids/antagonists & inhibitors , Glycerides/antagonists & inhibitors , Male , Nucleus Accumbens/physiology , Polyunsaturated Alkamides/pharmacology , Rats , Rats, Wistar , Receptor, Cannabinoid, CB1/drug effects , Receptor, Cannabinoid, CB1/metabolism , Rimonabant/pharmacology , Self Administration , Sulfonamides/pharmacology
4.
Addict Biol ; 24(6): 1204-1215, 2019 11.
Article in English | MEDLINE | ID: mdl-30421483

ABSTRACT

Deletion of fatty acid amide hydrolase (FAAH), enzyme responsible for degrading endocannabinoids, increases alcohol consumption and preference. However, there is a lack of data on neurochemical events in mice exposed to alcohol in the absence of FAAH. Extracellular levels of endocannabinoids and relevant neurotransmitters were measured by in vivo microdialysis in the nucleus accumbens (NAc) of FAAH knockout (KO) and wild-type (WT) mice during an ethanol (EtOH; 2 g/kg, ip) challenge in EtOH-naive and repeated (r) EtOH-treated mice. In both genotypes, EtOH treatment caused no changes in baseline endocannabinoid levels, although FAAH KO mice displayed higher baseline N-arachidonoylethanolamine levels than WT mice. EtOH challenge caused a sustained increase in 2-arachidonoylglycerol (2-AG) levels in EtOH-naive WT mice but not in FAAH KO mice. In contrast, 2-AG levels were decreased following EtOH challenge in (r)EtOH-treated mice in both genotypes. Whereas (r)EtOH-treated mice showed higher baseline dopamine and serotonin levels than EtOH-naive mice in WT mice, these differences were attenuated in FAAH KO mice. Significant differences in baseline γ-aminobutyric acid (GABA) and glutamate levels by EtOH history were observed in WT mice but not in FAAH KO mice. Moreover, opposed effects on glutamate response were observed after EtOH challenge in EtOH-naive and (r)EtOH-treated FAAH KO mice. Finally, FAAH deletion failed to show EtOH-induced locomotion sensitivity. These data provide evidence of a potential influence of 2-AG in the neurochemical response to EtOH exposure in the NAc.


Subject(s)
Amidohydrolases/genetics , Central Nervous System Depressants/pharmacology , Endocannabinoids/metabolism , Ethanol/pharmacology , Nucleus Accumbens/drug effects , Animals , Arachidonic Acids/metabolism , Behavior, Animal , Dopamine/metabolism , Glutamic Acid/drug effects , Glutamic Acid/metabolism , Glycerides/metabolism , Locomotion , Mice , Mice, Knockout , Microdialysis , Nucleus Accumbens/metabolism , Polyunsaturated Alkamides , Serotonin/metabolism , gamma-Aminobutyric Acid/drug effects , gamma-Aminobutyric Acid/metabolism
5.
Proc Natl Acad Sci U S A ; 113(4): 1086-91, 2016 Jan 26.
Article in English | MEDLINE | ID: mdl-26755579

ABSTRACT

Chronic nicotine exposure (CNE) alters synaptic transmission in the ventral tegmental area (VTA) in a manner that enhances dopaminergic signaling and promotes nicotine use. The present experiments identify a correlation between enhanced production of the endogenous cannabinoid 2-arachidonoylglycerol (2-AG) and diminished release of the inhibitory neurotransmitter GABA in the VTA following CNE. To study the functional role of on-demand 2-AG signaling in GABAergic synapses, we used 1,2,3-triazole urea compounds to selectively inhibit 2-AG biosynthesis by diacylglycerol lipase (DAGL). The potency and selectivity of these inhibitors were established in rats in vitro (rat brain proteome), ex vivo (brain slices), and in vivo (intracerebroventricular administration) using activity-based protein profiling and targeted metabolomics analyses. Inhibition of DAGL (2-AG biosynthesis) rescues nicotine-induced VTA GABA signaling following CNE. Conversely, enhancement of 2-AG signaling in naïve rats by inhibiting 2-AG degradation recapitulates the loss of nicotine-induced GABA signaling evident following CNE. DAGL inhibition reduces nicotine self-administration without disrupting operant responding for a nondrug reinforcer or motor activity. Collectively, these findings provide a detailed characterization of selective inhibitors of rat brain DAGL and demonstrate that excessive 2-AG signaling contributes to a loss of inhibitory GABAergic constraint of VTA excitability following CNE.


Subject(s)
Lipoprotein Lipase/antagonists & inhibitors , Nicotine/pharmacology , Ventral Tegmental Area/drug effects , Animals , Arachidonic Acids/analysis , Arachidonic Acids/antagonists & inhibitors , Arachidonic Acids/physiology , Endocannabinoids/analysis , Endocannabinoids/antagonists & inhibitors , Endocannabinoids/physiology , Glycerides/analysis , Glycerides/antagonists & inhibitors , Glycerides/physiology , Male , Rats , Rats, Wistar , Self Administration , Ventral Tegmental Area/physiology , gamma-Aminobutyric Acid/metabolism
6.
J Neurosci ; 37(7): 1853-1861, 2017 02 15.
Article in English | MEDLINE | ID: mdl-28202787

ABSTRACT

Persons with alcoholism who are abstinent exhibit persistent impairments in the capacity for response inhibition, and this form of impulsivity is significantly associated with heightened relapse risk. Brain-imaging studies implicate aberrant prefrontal cortical function in this behavioral pathology, although the underlying mechanisms are not understood. Here we present evidence that deficient activation of glycine and serine release in the ventral medial prefrontal cortex (vmPFC) contributes to increased motor impulsivity during protracted abstinence from long-term alcohol exposure. Levels of 12 neurotransmitters were monitored in the rat vmPFC during the performance of a challenging variant of the five-choice serial reaction time task (5-CSRTT) in which alcohol-exposed rats exhibit excessive premature responding. Following long-term ethanol exposure, rats showed blunted task-related recruitment of vmPFC glycine and serine release, and the loss of an inverse relationship between levels of these neurotransmitters and premature responding normally evident in alcohol-naive subjects. Intra-vmPFC administration of the glycine transport inhibitor ALX5407 prevented excessive premature responding by alcohol-exposed rats, and this was reliant on NMDA glycine site availability. Alcohol-exposed rats and controls did not differ in their premature responding and glycine and serine levels in vmPFC during the performance of the standard 5-CSRTT. Collectively, these findings provide novel insight into cortical neurochemical mechanisms contributing to increased impulsivity following long-term alcohol exposure and highlight the NMDA receptor coagonist site as a potential therapeutic target for increased impulsivity that may contribute to relapse risk.SIGNIFICANCE STATEMENT Persons with alcoholism demonstrate increased motor impulsivity during abstinence; however, the neuronal mechanisms underlying these behavioral effects remain unknown. Here, we took advantage of an animal model that shows deficiencies in inhibitory control following prolonged alcohol exposure to investigate the neurotransmitters that are potentially responsible for dysregulated motor impulsivity following long-term alcohol exposure. We found that increased motor impulsivity is associated with reduced recruitment of glycine and serine neurotransmitters in the ventromedial prefrontal cortex (vmPFC) cortex in rats following long-term alcohol exposure. Administration of glycine transport inhibitor ALX5407 in the vmPFC alleviated deficits in impulse control.


Subject(s)
Alcohol Abstinence , Alcohol Drinking/physiopathology , Glycine/metabolism , Impulsive Behavior/physiology , Signal Transduction/physiology , Animals , Central Nervous System Depressants/adverse effects , Choice Behavior/drug effects , Disease Models, Animal , Ethanol/adverse effects , Excitatory Amino Acid Antagonists/pharmacology , Excitatory Amino Acid Transporter 2/antagonists & inhibitors , Male , Neurotransmitter Agents/metabolism , Photic Stimulation , Quinolones/pharmacology , Rats , Rats, Wistar , Reaction Time/drug effects , Sarcosine/analogs & derivatives , Sarcosine/pharmacology , Serine/metabolism , Serine/pharmacology , Signal Transduction/drug effects
7.
J Virol ; 91(14)2017 07 15.
Article in English | MEDLINE | ID: mdl-28446679

ABSTRACT

Central nervous system infection of neonatal and adult rats with Borna disease virus (BDV) results in neuronal destruction and behavioral abnormalities with differential immune-mediated involvement. Neuroactive metabolites generated from the kynurenine pathway of tryptophan degradation have been implicated in several human neurodegenerative disorders. Here, we report that brain expression of key enzymes in the kynurenine pathway are significantly, but differentially, altered in neonatal and adult rats with BDV infection. Gene expression analysis of rat brains following neonatal infection showed increased expression of kynurenine amino transferase II (KATII) and kynurenine-3-monooxygenase (KMO) enzymes. Additionally, indoleamine 2,3-dioxygenase (IDO) expression was only modestly increased in a brain region- and time-dependent manner in neonatally infected rats; however, its expression was highly increased in adult infected rats. The most dramatic impact on gene expression was seen for KMO, whose activity promotes the production of neurotoxic quinolinic acid. KMO expression was persistently elevated in brain regions of both newborn and adult BDV-infected rats, with increases reaching up to 86-fold. KMO protein levels were increased in neonatally infected rats and colocalized with neurons, the primary target cells of BDV infection. Furthermore, quinolinic acid was elevated in neonatally infected rat brains. We further demonstrate increased expression of KATII and KMO, but not IDO, in vitro in BDV-infected C6 astroglioma cells. Our results suggest that BDV directly impacts the kynurenine pathway, an effect that may be exacerbated by inflammatory responses in immunocompetent hosts. Thus, experimental models of BDV infection may provide new tools for discriminating virus-mediated from immune-mediated impacts on the kynurenine pathway and their relative contribution to neurodegeneration.IMPORTANCE BDV causes persistent, noncytopathic infection in vitro yet still elicits widespread neurodegeneration of infected neurons in both immunoincompetent and immunocompetent hosts. Here, we show that BDV infection induces expression of key enzymes of the kynurenine pathway in brains of newborn and adult infected rats and cultured astroglioma cells, shunting tryptophan degradation toward the production of neurotoxic quinolinic acid. Thus, our findings newly implicate this metabolic pathway in BDV-induced neurodegeneration. Given the importance of the kynurenine pathway in a wide range of human infections and neurodegenerative and neuropsychiatric disorders, animal models of BDV infection may serve as important tools for contrasting direct viral and indirect antiviral immune-mediated impacts on kynurenine pathway dysregulation and the ensuing neurodevelopmental and neuropathological consequences.


Subject(s)
Borna Disease/physiopathology , Borna disease virus/growth & development , Brain/pathology , Host-Pathogen Interactions , Kynurenine/metabolism , Metabolic Networks and Pathways , Quinolinic Acid/toxicity , Animals , Borna Disease/pathology , Cell Line , Disease Models, Animal , Gene Expression Profiling , Gene Expression Regulation , Rats
8.
Addict Biol ; 23(5): 1117-1129, 2018 09.
Article in English | MEDLINE | ID: mdl-28940879

ABSTRACT

Repeated cycles of alcohol [ethanol (EtOH)] intoxication and withdrawal dysregulate excitatory glutamatergic systems in the brain and induce neuroadaptations in the medial prefrontal cortex (mPFC) that contribute to cognitive dysfunction. The mPFC is composed of subdivisions that are functionally distinct, with dorsal regions facilitating drug-cue associations and ventral regions modulating new learning in the absence of drug. A key modulator of glutamatergic activity is the holoenzyme calcium/calmodulin-dependent protein kinase II (CaMKII) that phosphorylates ionotropic glutamate receptors. Here, we examined the hypothesis that abstinence from chronic intermittent EtOH (CIE) exposure dysregulates CaMKII activity in the mPFC to impair cognitive flexibility. We used an operant model of strategy set shifting in male Long-Evans rats demonstrating reduced susceptibility to trial omissions during performance in a visual cue-guided task versus albino strains. Relative to naïve controls, rats experiencing approximately 10 days of abstinence from CIE vapor exposure demonstrated impaired performance during a procedural shift from visual cue to spatial location discrimination. Phosphorylation of CaMKII subtype α was upregulated in the dorsal, but not ventral mPFC of CIE-exposed rats, and was positively correlated with perseverative-like responding during the set shift. The findings suggest that abstinence from CIE exposure induces an undercurrent of kinase activity (e.g. CaMKII), which may promote aberrant glutamatergic responses in select regions of the mPFC. Given the role of the mPFC in modulating executive control of behavior, we propose that increased CaMKII subtype α activity reflects a dysregulated 'top-down' circuit that interferes with adaptive behavioral performance under changing environmental demands.


Subject(s)
Alcoholism/complications , Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism , Cognitive Dysfunction/etiology , Ethanol/pharmacology , Prefrontal Cortex/metabolism , Alcoholism/genetics , Alcoholism/physiopathology , Animals , Behavior, Animal/drug effects , Calcium-Calmodulin-Dependent Protein Kinase Type 2/genetics , Central Nervous System Depressants/pharmacology , Cognition/drug effects , Cognitive Dysfunction/physiopathology , Disease Models, Animal , Male , Phosphorylation , Rats , Rats, Long-Evans
9.
Addict Biol ; 23(6): 1251-1261, 2018 11.
Article in English | MEDLINE | ID: mdl-30421559

ABSTRACT

A dual probe microdialysis study was designed to characterize GABA and dopamine (DA) release in the basal ganglia of cannabinoid-dependent Wistar rats. Whereas chronic administration of the cannabinoid receptor agonist WIN55,212 (WIN) resulted in increased basal GABA release, the D2 agonist receptor-mediated control of GABA and DA release elicited by quinpirole was similar in both cannabinoid-dependent and non dependent animals. However, quinpirole did induce a greater number of more stereotypies in cannabinoid-dependent animals, indicating a dysregulated behavioral response.


Subject(s)
Cannabinoids/pharmacology , Dopamine/metabolism , gamma-Aminobutyric Acid/metabolism , Analysis of Variance , Animals , Basal Ganglia/metabolism , Benzoxazines/pharmacology , Calcium Channel Blockers/pharmacology , Cannabinoids/administration & dosage , Dopamine Agonists/pharmacology , Endocannabinoids/metabolism , Globus Pallidus/drug effects , Globus Pallidus/metabolism , Glutamic Acid/metabolism , Male , Microdialysis , Morpholines/pharmacology , Naphthalenes/pharmacology , Quinpirole/pharmacology , RNA, Messenger/metabolism , Rats, Wistar , Real-Time Polymerase Chain Reaction , Substance-Related Disorders
10.
Addict Biol ; 23(2): 723-734, 2018 03.
Article in English | MEDLINE | ID: mdl-28660730

ABSTRACT

Nicotine exerts its rewarding effects by promoting an increase in dopamine (DA) release in the nucleus accumbens (NAc), and this process is influenced by the endocannabinoid system. Fatty acid amide hydrolase (FAAH) is the main enzyme responsible for the degradation of the endocannabinoid anandamide and other non-cannabinoid N-acylethanolamines. Previous research has reported that both genetic deletion and pharmacological inhibition of FAAH enhance nicotine-induced conditioned place preference at low doses. We conducted a microdialysis study to characterize nicotine-induced changes in DA and serotonin (5-HT) levels in the NAc of FAAH knockout (KO) mice using a conditioned place preference-like paradigm with three nicotine doses (0.1, 1 and 10 mg/kg, s.c.). Additionally, the effects of the selective FAAH inhibitor PF-3845 (10 mg/kg, i.p.) were also examined. Our data indicated that compared with wild-type mice, genetic deletion of FAAH selectively enhanced the effect of low-dose nicotine on DA release (p < 0.001) and resulted in a strong post-nicotine elevation in DA levels (p < 0.01). However, there were no differences between the genotypes at higher doses. Furthermore, FAAH KO mice displayed a moderate enhancement of the effect of low-dose nicotine on NAc 5-HT release (p < 0.05), with no differences between the genotypes at higher doses. Compared with vehicle-pretreated mice, mice pretreated with PF-3845 displayed an enhancement of the effect of low-dose nicotine on NAc DA release (p < 0.001), which resulted in a sustained increase in DA levels (p < 0.05). Similar to FAAH KO mice, PF-3845-pretreated mice displayed a moderate enhancement of the effect of low-dose nicotine on NAc 5-HT release (p < 0.01). These observations in mice suggest that enhanced nicotine-induced NAc DA release might contribute to increased sensitivity to the conditioned rewarding effects of low-dose nicotine following FAAH inhibition, which has been previously reported. Future studies combining behavioral and neurochemical approaches are needed to elucidate the precise mechanism of these effects.


Subject(s)
Amidohydrolases/genetics , Dopamine/metabolism , Nicotine/pharmacology , Nicotinic Agonists/pharmacology , Nucleus Accumbens/drug effects , Amidohydrolases/antagonists & inhibitors , Amidohydrolases/metabolism , Animals , Endocannabinoids/metabolism , Mice , Mice, Knockout , Microdialysis , Nucleus Accumbens/metabolism , Piperidines/pharmacology , Pyridines/pharmacology
11.
Proc Natl Acad Sci U S A ; 112(22): 7091-6, 2015 Jun 02.
Article in English | MEDLINE | ID: mdl-25964320

ABSTRACT

G protein-gated inwardly rectifying potassium (GIRK) channels are critical regulators of neuronal excitability and can be directly activated by ethanol. Constitutive deletion of the GIRK3 subunit has minimal phenotypic consequences, except in response to drugs of abuse. Here we investigated how the GIRK3 subunit contributes to the cellular and behavioral effects of ethanol, as well as to voluntary ethanol consumption. We found that constitutive deletion of GIRK3 in knockout (KO) mice selectively increased ethanol binge-like drinking, without affecting ethanol metabolism, sensitivity to ethanol intoxication, or continuous-access drinking. Virally mediated expression of GIRK3 in the ventral tegmental area (VTA) reversed the phenotype of GIRK3 KO mice and further decreased the intake of their wild-type counterparts. In addition, GIRK3 KO mice showed a blunted response of the mesolimbic dopaminergic (DA) pathway to ethanol, as assessed by ethanol-induced excitation of VTA neurons and DA release in the nucleus accumbens. These findings support the notion that the subunit composition of VTA GIRK channels is a critical determinant of DA neuron sensitivity to drugs of abuse. Furthermore, our study reveals the behavioral impact of this cellular effect, whereby the level of GIRK3 expression in the VTA tunes ethanol intake under binge-type conditions: the more GIRK3, the less ethanol drinking.


Subject(s)
Dopaminergic Neurons/metabolism , Ethanol/pharmacology , G Protein-Coupled Inwardly-Rectifying Potassium Channels/metabolism , Ion Channel Gating/physiology , Motivation/genetics , Analysis of Variance , Animals , Binge Drinking/genetics , DNA Primers/genetics , G Protein-Coupled Inwardly-Rectifying Potassium Channels/deficiency , In Situ Hybridization , Ion Channel Gating/genetics , Mice , Mice, Inbred C57BL , Mice, Knockout , Microdialysis , Reverse Transcriptase Polymerase Chain Reaction , Reward
12.
Am J Physiol Endocrinol Metab ; 311(3): E605-19, 2016 09 01.
Article in English | MEDLINE | ID: mdl-27436613

ABSTRACT

This review summarizes the American Physiological Society (APS) Presidential Symposium 1 entitled "Physiological Processes Underlying Organ Injury in Alcohol Abuse" at the 2016 Experimental Biology meeting. The symposium was organized by Dr. Patricia Molina, past president of the APS, was held on April 3 at the Convention Center in San Diego, CA, and was funded by the National Institute on Alcohol Abuse and Alcoholism. The "Physiological Processes Underlying Organ Injury in Alcohol Abuse Symposium" assembled experts and leaders in the field and served as a platform to discuss and share knowledge on the latest developments and scientific advances on the mechanisms underlying organ injury in alcohol abuse. This symposium provided unique, interdisciplinary alcohol research, including several organs, liver, muscle, adipose, and brain, affected by excessive alcohol use.


Subject(s)
Alcoholism/pathology , Adipose Tissue/pathology , Animals , Brain/pathology , Endocannabinoids/metabolism , Humans , Liver/pathology , Muscular Atrophy/etiology , Muscular Atrophy/pathology
13.
Addict Biol ; 21(4): 788-801, 2016 07.
Article in English | MEDLINE | ID: mdl-25940135

ABSTRACT

The endogenous cannabinoids (eCBs) influence the acute response to ethanol and the development of tolerance, dependence and relapse. Chronic alcohol exposure alters eCB levels and Type 1 cannabinoid receptor (CB1 ) expression and function in brain regions associated with addiction. CB1 inhibits GABA release, and GABAergic dysregulation in the central nucleus of the amygdala (CeA) is critical in the transition to alcohol dependence. We investigated possible disruptions in CB1 signaling of rat CeA GABAergic transmission following intermittent ethanol exposure. In the CeA of alcohol-naive rats, CB1 agonist WIN 55,212-2 (WIN) decreased the frequency of spontaneous and miniature GABAA receptor-mediated inhibitory postsynaptic currents (s/mIPSCs). This effect was prevented by CB1 antagonism, but not Type 2 cannabinoid receptor (CB2 ) antagonism. After 2-3 weeks of intermittent ethanol exposure, these WIN inhibitory effects were attenuated, suggesting ethanol-induced impairments in CB1 function. The CB1 antagonist AM251 revealed a tonic eCB/CB1 control of GABAergic transmission in the alcohol-naive CeA that was occluded by calcium chelation in the postsynaptic cell. Chronic ethanol exposure abolished this tonic CB1 influence on mIPSC, but not sIPSC, frequency. Finally, acute ethanol increased CeA GABA release in both naive and ethanol-exposed rats. Although CB1 activation prevented this effect, the AM251- and ethanol-induced GABA release were additive, ruling out a direct participation of CB1 signaling in the ethanol effect. Collectively, these observations demonstrate an important CB1 influence on CeA GABAergic transmission and indicate that the CeA is particularly sensitive to alcohol-induced disruptions of CB1 signaling.


Subject(s)
Alcoholism/physiopathology , Central Amygdaloid Nucleus/drug effects , Ethanol/pharmacology , GABAergic Neurons/drug effects , Receptor, Cannabinoid, CB1/drug effects , Receptors, GABA-A/drug effects , Animals , Central Amygdaloid Nucleus/physiopathology , Central Nervous System Depressants/pharmacology , Chronic Disease , Disease Models, Animal , Male , Rats , Rats, Sprague-Dawley , Synapses/drug effects
14.
Addict Biol ; 21(4): 859-72, 2016 07.
Article in English | MEDLINE | ID: mdl-26037332

ABSTRACT

Oleoylethanolamide (OEA) is a satiety factor that controls motivational responses to dietary fat. Here we show that alcohol administration causes the release of OEA in rodents, which in turn reduces alcohol consumption by engaging peroxisome proliferator-activated receptor-alpha (PPAR-α). This effect appears to rely on peripheral signaling mechanisms as alcohol self-administration is unaltered by intracerebral PPAR-α agonist administration, and the lesion of sensory afferent fibers (by capsaicin) abrogates the effect of systemically administered OEA on alcohol intake. Additionally, OEA is shown to block cue-induced reinstatement of alcohol-seeking behavior (an animal model of relapse) and reduce the severity of somatic withdrawal symptoms in alcohol-dependent animals. Collectively, these findings demonstrate a homeostatic role for OEA signaling in the behavioral effects of alcohol exposure and highlight OEA as a novel therapeutic target for alcohol use disorders and alcoholism.


Subject(s)
Alcohol Drinking/metabolism , Alcohol Drinking/psychology , Alcoholism/metabolism , Alcoholism/psychology , Endocannabinoids/pharmacology , Oleic Acids/pharmacology , Satiety Response/physiology , Animals , Disease Models, Animal , Male , Mice , PPAR alpha/drug effects , PPAR alpha/metabolism , Rats, Wistar , Signal Transduction/drug effects
15.
Proc Natl Acad Sci U S A ; 110(27): E2518-27, 2013 Jul 02.
Article in English | MEDLINE | ID: mdl-23776240

ABSTRACT

Synaptic loss is the cardinal feature linking neuropathology to cognitive decline in Alzheimer's disease (AD). However, the mechanism of synaptic damage remains incompletely understood. Here, using FRET-based glutamate sensor imaging, we show that amyloid-ß peptide (Aß) engages α7 nicotinic acetylcholine receptors to induce release of astrocytic glutamate, which in turn activates extrasynaptic NMDA receptors (eNMDARs) on neurons. In hippocampal autapses, this eNMDAR activity is followed by reduction in evoked and miniature excitatory postsynaptic currents (mEPSCs). Decreased mEPSC frequency may reflect early synaptic injury because of concurrent eNMDAR-mediated NO production, tau phosphorylation, and caspase-3 activation, each of which is implicated in spine loss. In hippocampal slices, oligomeric Aß induces eNMDAR-mediated synaptic depression. In AD-transgenic mice compared with wild type, whole-cell recordings revealed excessive tonic eNMDAR activity accompanied by eNMDAR-sensitive loss of mEPSCs. Importantly, the improved NMDAR antagonist NitroMemantine, which selectively inhibits extrasynaptic over physiological synaptic NMDAR activity, protects synapses from Aß-induced damage both in vitro and in vivo.


Subject(s)
Amyloid beta-Peptides/toxicity , Astrocytes/metabolism , Glutamic Acid/metabolism , Neural Inhibition/physiology , Peptide Fragments/toxicity , Receptors, N-Methyl-D-Aspartate/metabolism , Synapses/pathology , Alzheimer Disease/metabolism , Alzheimer Disease/pathology , Animals , Astrocytes/pathology , Coculture Techniques , Female , Fluorescence Resonance Energy Transfer , HEK293 Cells , Hippocampus/metabolism , Hippocampus/pathology , Humans , Male , Mice , Mice, Transgenic , Rats , Receptors, Nicotinic/metabolism , Synapses/metabolism , alpha7 Nicotinic Acetylcholine Receptor
16.
Addict Biol ; 20(2): 263-74, 2015 Mar.
Article in English | MEDLINE | ID: mdl-24341858

ABSTRACT

Impulsivity is a risk factor for alcoholism, and long-term alcohol exposure may further impair impulse control in a manner that propels problematic alcohol use. The present study employed the rat 5-choice serial reaction time task (5-CSRTT) to measure behavioral inhibition and attentional capacity during abstinence from repeated 5-day cycles of alcohol liquid diet consumption. Task performance was not disrupted following the first cycle of alcohol exposure; however, evidence of impaired behavioral inhibition emerged following the third cycle of alcohol exposure. In comparison with controls, alcoholic rats exhibited deficits in inhibitory control during cognitively challenging 5-CSRTT tests employing variable intertrial interval (varITI). This behavioral disruption was not present during early abstinence (3 days) but was evident by 7 days of abstinence and persisted for at least 34 days. Interestingly, renewed alcohol consumption ameliorated these disruptions in impulse control, although deficient behavioral inhibition re-emerged during subsequent abstinence. Indices of increased impulsivity were no longer present in tests conducted after 49 days of abstinence. Alcohol-related impairments in impulse control were not evident in sessions employing highly familiar task parameters regardless of the abstinence period, and control experiments confirmed that performance deficits during the challenge sessions were unlikely to result from alcohol-related disruption in the adaptation to repeated varITI testing. Together, the current findings demonstrate that chronic intermittent alcohol consumption results in decreased behavioral inhibition in rats that is temporally similar to clinical observations of disrupted impulsive control in abstinent alcoholics performing tasks of behavioral inhibition.


Subject(s)
Alcohol Abstinence , Alcoholic Intoxication , Attention , Impulsive Behavior , Inhibition, Psychological , Animals , Rats , Rats, Wistar
17.
Int J Neuropsychopharmacol ; 17(3): 455-68, 2014 Mar.
Article in English | MEDLINE | ID: mdl-24169105

ABSTRACT

Oleoylethanolamide (OEA) is an agonist of the peroxisome proliferator-activated receptor α (PPARα) and has been described to exhibit neuroprotective properties when administered locally in animal models of several neurological disorder models, including stroke and Parkinson's disease. However, there is little information regarding the effectiveness of systemic administration of OEA on Parkinson's disease. In the present study, OEA-mediated neuroprotection has been tested on in vivo and in vitro models of 6-hydroxydopamine (6-OH-DA)-induced degeneration. The in vivo model was based on the intrastriatal infusion of the neurotoxin 6-OH-DA, which generates Parkinsonian symptoms. Rats were treated 2 h before and after the 6-OH-DA treatment with systemic OEA (0.5, 1, and 5 mg/kg). The Parkinsonian symptoms were evaluated at 1 and 4 wk after the development of lesions. The functional status of the nigrostriatal system was studied through tyrosine-hydroxylase (TH) and hemeoxygenase-1 (HO-1, oxidation marker) immunostaining as well as by monitoring the synaptophysin content. In vitro cell cultures were also treated with OEA and 6-OH-DA. As expected, our results revealed 6-OH-DA induced neurotoxicity and behavioural deficits; however, these alterations were less severe in the animals treated with the highest dose of OEA (5 mg/kg). 6-OH-DA administration significantly reduced the striatal TH-immunoreactivity (ir) density, synaptophysin expression, and the number of nigral TH-ir neurons. Moreover, 6-OH-DA enhanced striatal HO-1 content, which was blocked by OEA (5 mg/kg). In vitro, 0.5 and 1 µM of OEA exerted significant neuroprotection on cultured nigral neurons. These effects were abolished after blocking PPARα with the selective antagonist GW6471. In conclusion, systemic OEA protects the nigrostriatal circuit from 6-OH-DA-induced neurotoxicity through a PPARα-dependent mechanism.


Subject(s)
Corpus Striatum/drug effects , Neuroprotective Agents/administration & dosage , Oleic Acids/administration & dosage , Parkinsonian Disorders/drug therapy , Parkinsonian Disorders/pathology , Substantia Nigra/drug effects , Animals , Cells, Cultured , Corpus Striatum/cytology , Corpus Striatum/metabolism , Disease Models, Animal , Dose-Response Relationship, Drug , Endocannabinoids , Heme Oxygenase-1/metabolism , L-Lactate Dehydrogenase/metabolism , Male , Motor Activity/drug effects , Neurons/drug effects , Neurons/metabolism , Neurotoxins/toxicity , Oxidopamine/toxicity , Parkinsonian Disorders/chemically induced , Parkinsonian Disorders/physiopathology , Rats , Rats, Wistar , Substantia Nigra/cytology , Substantia Nigra/metabolism , Synaptophysin/metabolism , Tyrosine 3-Monooxygenase/metabolism
18.
J Neurochem ; 123(4): 578-88, 2012 Nov.
Article in English | MEDLINE | ID: mdl-22905672

ABSTRACT

Adolescent smokers report enhanced positive responses to tobacco and fewer negative effects of withdrawal from this drug than adults, and this is believed to propel higher tobacco use during adolescence. Differential dopaminergic responses to nicotine are thought to underlie these age-related effects, as adolescent rats experience lower withdrawal-related deficits in nucleus accumbens (NAcc) dopamine versus adults. This study examined whether age differences in NAcc dopamine during withdrawal are mediated by excitatory or inhibitory transmission in the ventral tegmental area (VTA) dopamine cell body region. In vivo microdialysis was used to monitor extracellular levels of glutamate and gamma-aminobutyric acid (GABA) in the VTA of adolescent and adult rats experiencing nicotine withdrawal. In adults, nicotine withdrawal produced decreases in VTA glutamate levels (44% decrease) and increases in VTA GABA levels (38% increase). In contrast, adolescents did not exhibit changes in either of these measures. Naïve controls of both ages did not display changes in NAcc dopamine, VTA glutamate, or VTA GABA following mecamylamine. These results indicate that adolescents display resistance to withdrawal-related neurochemical processes that inhibit mesolimbic dopamine function in adults experiencing nicotine withdrawal. Our findings provide a potential mechanism involving VTA amino acid neurotransmission that modulates age differences during withdrawal.


Subject(s)
Adaptation, Physiological/physiology , Dopamine/metabolism , Limbic System/metabolism , Nicotine/adverse effects , Nicotinic Agonists/adverse effects , Substance Withdrawal Syndrome/pathology , Adaptation, Physiological/drug effects , Age Factors , Analysis of Variance , Animals , Chromatography, High Pressure Liquid , Dialysis , Electrochemistry , Glutamic Acid/metabolism , Limbic System/drug effects , Male , Mecamylamine/pharmacology , Nicotinic Antagonists/pharmacology , Nucleus Accumbens/drug effects , Nucleus Accumbens/metabolism , Rats , Rats, Wistar , Statistics as Topic , Ventral Tegmental Area/drug effects , Ventral Tegmental Area/metabolism , gamma-Aminobutyric Acid/metabolism
19.
Alcohol Clin Exp Res ; 36(6): 984-94, 2012 Jun.
Article in English | MEDLINE | ID: mdl-22141465

ABSTRACT

BACKGROUND: Endogenous cannabinoids such as anandamide and 2-arachidonoylglycerol (2-AG) exert important regulatory influences on neuronal signaling, participate in short- and long-term forms of neuroplasticity, and modulate stress responses and affective behavior in part through the modulation of neurotransmission in the amygdala. Alcohol consumption alters brain endocannabinoid levels, and alcohol dependence is associated with dysregulated amygdalar function, stress responsivity, and affective control. METHODS: The consequence of long-term alcohol consumption on the expression of genes related to endocannabinoid signaling was investigated using quantitative RT-PCR analyses of amygdala tissue. Two groups of ethanol (EtOH)-exposed rats were generated by maintenance on an EtOH liquid diet (10%): the first group received continuous access to EtOH for 15 days, whereas the second group was given intermittent access to the EtOH diet (5 d/wk for 3 weeks). Control subjects were maintained on an isocaloric EtOH-free liquid diet. To provide an initial profile of acute withdrawal, amygdala tissue was harvested following either 6 or 24 hours of EtOH withdrawal. RESULTS: Acute EtOH withdrawal was associated with significant changes in mRNA expression for various components of the endogenous cannabinoid system in the amygdala. Specifically, reductions in mRNA expression for the primary clearance routes for anandamide and 2-AG (fatty acid amide hydrolase [FAAH] and monoacylglycerol lipase [MAGL], respectively) were evident, as were reductions in mRNA expression for CB(1) , CB(2) , and GPR55 receptors. Although similar alterations in FAAH mRNA were evident following either continuous or intermittent EtOH exposure, alterations in MAGL and cannabinoid receptor-related mRNA (e.g., CB(1) , CB(2) , GPR55) were more pronounced following intermittent exposure. In general, greater withdrawal-associated deficits in mRNA expression were evident following 24 versus 6 hours of withdrawal. No significant changes in mRNA expression for enzymes involved in 2-AG biosynthesis (e.g., diacylglicerol lipase-α/ß) were found in any condition. CONCLUSIONS: These findings suggest that EtOH dependence and withdrawal are associated with dysregulated endocannabinoid signaling in the amygdala. These alterations may contribute to withdrawal-related dysregulation of amygdalar neurotransmission.


Subject(s)
Amygdala/drug effects , Cannabinoid Receptor Modulators/metabolism , Central Nervous System Depressants/adverse effects , Endocannabinoids , Ethanol/adverse effects , Gene Expression/drug effects , RNA, Messenger/drug effects , Substance Withdrawal Syndrome , Amidohydrolases/drug effects , Amidohydrolases/genetics , Amidohydrolases/metabolism , Amygdala/metabolism , Animals , Gene Expression Profiling , Male , Monoacylglycerol Lipases/drug effects , Monoacylglycerol Lipases/genetics , Monoacylglycerol Lipases/metabolism , Rats , Rats, Wistar , Real-Time Polymerase Chain Reaction , Receptors, Cannabinoid/drug effects , Receptors, Cannabinoid/genetics , Receptors, Cannabinoid/metabolism , Signal Transduction/drug effects , Signal Transduction/genetics
20.
Nat Chem Biol ; 5(1): 37-44, 2009 Jan.
Article in English | MEDLINE | ID: mdl-19029917

ABSTRACT

2-Arachidonoylglycerol (2-AG) and anandamide are endocannabinoids that activate the cannabinoid receptors CB1 and CB2. Endocannabinoid signaling is terminated by enzymatic hydrolysis, a process that for anandamide is mediated by fatty acid amide hydrolase (FAAH), and for 2-AG is thought to involve monoacylglycerol lipase (MAGL). FAAH inhibitors produce a select subset of the behavioral effects observed with CB1 agonists, which suggests a functional segregation of endocannabinoid signaling pathways in vivo. Testing this hypothesis, however, requires specific tools to independently block anandamide and 2-AG metabolism. Here, we report a potent and selective inhibitor of MAGL called JZL184 that, upon administration to mice, raises brain 2-AG by eight-fold without altering anandamide. JZL184-treated mice exhibited a broad array of CB1-dependent behavioral effects, including analgesia, hypothermia and hypomotility. These data indicate that 2-AG endogenously modulates several behavioral processes classically associated with the pharmacology of cannabinoids and point to overlapping and unique functions for 2-AG and anandamide in vivo.


Subject(s)
Arachidonic Acids/metabolism , Behavior, Animal/drug effects , Cannabinoids , Glycerides/metabolism , Amidohydrolases/antagonists & inhibitors , Animals , Behavior, Animal/physiology , Benzodioxoles/chemistry , Benzodioxoles/pharmacology , Dose-Response Relationship, Drug , Endocannabinoids , Hydrolysis/drug effects , Male , Mice , Mice, Inbred C57BL , Monoacylglycerol Lipases/antagonists & inhibitors , Piperidines/chemistry , Piperidines/pharmacology , Receptor, Cannabinoid, CB1/metabolism , Receptor, Cannabinoid, CB2/metabolism
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