ABSTRACT
The ventrolateral subdivision of the ventromedial hypothalamus (VMHvl) contains â¼4,000 neurons that project to multiple targets and control innate social behaviors including aggression and mounting. However, the number of cell types in VMHvl and their relationship to connectivity and behavioral function are unknown. We performed single-cell RNA sequencing using two independent platforms-SMART-seq (â¼4,500 neurons) and 10x (â¼78,000 neurons)-and investigated correspondence between transcriptomic identity and axonal projections or behavioral activation, respectively. Canonical correlation analysis (CCA) identified 17 transcriptomic types (T-types), including several sexually dimorphic clusters, the majority of which were validated by seqFISH. Immediate early gene analysis identified T-types exhibiting preferential responses to intruder males versus females but only rare examples of behavior-specific activation. Unexpectedly, many VMHvl T-types comprise a mixed population of neurons with different projection target preferences. Overall our analysis revealed that, surprisingly, few VMHvl T-types exhibit a clear correspondence with behavior-specific activation and connectivity.
Subject(s)
Hypothalamus/cytology , Neurons/classification , Social Behavior , Animals , Estrogen Receptor alpha/genetics , Estrogen Receptor alpha/metabolism , Female , Hypothalamus/physiology , Male , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Neurons/metabolism , Neurons/physiology , Sexual Behavior, Animal , Single-Cell Analysis , TranscriptomeABSTRACT
Identifying the relationships between chromosome structures, nuclear bodies, chromatin states and gene expression is an overarching goal of nuclear-organization studies1-4. Because individual cells appear to be highly variable at all these levels5, it is essential to map different modalities in the same cells. Here we report the imaging of 3,660 chromosomal loci in single mouse embryonic stem (ES) cells using DNA seqFISH+, along with 17 chromatin marks and subnuclear structures by sequential immunofluorescence and the expression profile of 70 RNAs. Many loci were invariably associated with immunofluorescence marks in single mouse ES cells. These loci form 'fixed points' in the nuclear organizations of single cells and often appear on the surfaces of nuclear bodies and zones defined by combinatorial chromatin marks. Furthermore, highly expressed genes appear to be pre-positioned to active nuclear zones, independent of bursting dynamics in single cells. Our analysis also uncovered several distinct mouse ES cell subpopulations with characteristic combinatorial chromatin states. Using clonal analysis, we show that the global levels of some chromatin marks, such as H3 trimethylation at lysine 27 (H3K27me3) and macroH2A1 (mH2A1), are heritable over at least 3-4 generations, whereas other marks fluctuate on a faster time scale. This seqFISH+-based spatial multimodal approach can be used to explore nuclear organization and cell states in diverse biological systems.
Subject(s)
Cell Compartmentation/genetics , Cell Nucleus/genetics , Genomics/methods , Mouse Embryonic Stem Cells/cytology , Single-Cell Analysis/methods , Transcriptome/genetics , Animals , Cell Line , Chromatin/genetics , Chromatin/metabolism , Chromosomes, Mammalian/genetics , Clone Cells/cytology , Fluorescent Antibody Technique , Genetic Markers , Histones/metabolism , Lysine/metabolism , Male , Mice , Time FactorsABSTRACT
Diverse cell types in tissues have distinct gene expression programs, chromatin states, and nuclear architectures. To correlate such multimodal information across thousands of single cells in mouse brain tissue sections, we use integrated spatial genomics, imaging thousands of genomic loci along with RNAs and epigenetic markers simultaneously in individual cells. We reveal that cell typespecific association and scaffolding of DNA loci around nuclear bodies organize the nuclear architecture and correlate with differential expression levels in different cell types. At the submegabase level, active and inactive X chromosomes access similar domain structures in single cells despite distinct epigenetic and expression states. This work represents a major step forward in linking single-cell three-dimensional nuclear architecture, gene expression, and epigenetic modifications in a native tissue context.
Subject(s)
Cell Nucleus/metabolism , Cell Nucleus/ultrastructure , Cerebral Cortex/cytology , Neuroglia/ultrastructure , Neurons/ultrastructure , Single-Cell Analysis , Animals , Cerebral Cortex/metabolism , Chromatin/metabolism , Chromatin/ultrastructure , Chromosomes/metabolism , Chromosomes/ultrastructure , Endothelial Cells/metabolism , Endothelial Cells/ultrastructure , Epigenesis, Genetic , Female , Genome , In Situ Hybridization, Fluorescence , Mice , Neuroglia/metabolism , Neurons/metabolism , RNA-Seq , Transcription, Genetic , Transcriptome , X Chromosome/metabolism , X Chromosome/ultrastructureABSTRACT
Spatial transcriptomic and proteomic technologies have provided new opportunities to investigate cells in their native microenvironment. Here we present Giotto, a comprehensive and open-source toolbox for spatial data analysis and visualization. The analysis module provides end-to-end analysis by implementing a wide range of algorithms for characterizing tissue composition, spatial expression patterns, and cellular interactions. Furthermore, single-cell RNAseq data can be integrated for spatial cell-type enrichment analysis. The visualization module allows users to interactively visualize analysis outputs and imaging features. To demonstrate its general applicability, we apply Giotto to a wide range of datasets encompassing diverse technologies and platforms.