1.
Mikrobiologiia
; 48(1): 93-8, 1979.
Article
in Russian
| MEDLINE
| ID: mdl-106225
ABSTRACT
When Bacillus subtilis produces alpha-amylase in the course of continuous cultivation, it is difficult to maintain the activity at a constant level. This may be due to the formation of nonproductive mutants. Individual cells in the population have been analysed in the course of the continuous process. The composition of the population changes depending on time and the composition of the growth medium. Semisynthetic media cause selection of mutants which synthesize the enzyme at a low rate. In contrast, complex media which are more enriched in the sources of carbon and nitrogen induce accumulation of mutants with a high activity.