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1.
Nutrients ; 14(10)2022 May 13.
Article in English | MEDLINE | ID: mdl-35631195

ABSTRACT

Metabolic Syndrome (MetS) is a cluster of metabolic alterations mostly related to visceral adiposity, which in turn promotes glucose intolerance and a chronic systemic inflammatory state, characterized by immune cell infiltration. Such immune system activation increases the risk of severe disease subsequent to viral infections. Strong correlations between elevated body mass index (BMI), type-2-diabetes and increased risk of hospitalization after pandemic influenza H1N1 infection have been described. Similarly, a correlation between elevated blood glucose level and SARS-CoV-2 infection severity and mortality has been described, indicating MetS as an important predictor of clinical outcomes in patients with COVID-19. Adipose secretome, including two of the most abundant and well-studied adipokines, leptin and interleukin-6, is involved in the regulation of energy metabolism and obesity-related low-grade inflammation. Similarly, skeletal muscle hormones-called myokines-released in response to physical exercise affect both metabolic homeostasis and immune system function. Of note, several circulating hormones originate from both adipose tissue and skeletal muscle and display different functions, depending on the metabolic context. This review aims to summarize recent data in the field of exercise immunology, investigating the acute and chronic effects of exercise on myokines release and immune system function.


Subject(s)
COVID-19 , Influenza A Virus, H1N1 Subtype , Metabolic Syndrome , Exercise/physiology , Humans , Immunity , Inflammation , Nutritional Status , SARS-CoV-2
2.
Elife ; 102021 12 29.
Article in English | MEDLINE | ID: mdl-34964714

ABSTRACT

To identify and memorize discrete but similar environmental inputs, the brain needs to distinguish between subtle differences of activity patterns in defined neuronal populations. The Kenyon cells (KCs) of the Drosophila adult mushroom body (MB) respond sparsely to complex olfactory input, a property that is thought to support stimuli discrimination in the MB. To understand how this property emerges, we investigated the role of the inhibitory anterior paired lateral (APL) neuron in the input circuit of the MB, the calyx. Within the calyx, presynaptic boutons of projection neurons (PNs) form large synaptic microglomeruli (MGs) with dendrites of postsynaptic KCs. Combining electron microscopy (EM) data analysis and in vivo calcium imaging, we show that APL, via inhibitory and reciprocal synapses targeting both PN boutons and KC dendrites, normalizes odour-evoked representations in MGs of the calyx. APL response scales with the PN input strength and is regionalized around PN input distribution. Our data indicate that the formation of a sparse code by the KCs requires APL-driven normalization of their MG postsynaptic responses. This work provides experimental insights on how inhibition shapes sensory information representation in a higher brain centre, thereby supporting stimuli discrimination and allowing for efficient associative memory formation.


Subject(s)
Drosophila melanogaster/physiology , Mushroom Bodies/physiology , Neurons/ultrastructure , Smell/physiology , Animals , Calcium/analysis , Female , Male , Microscopy, Confocal , Microscopy, Electron , Mushroom Bodies/ultrastructure , Neurons/physiology , Presynaptic Terminals
3.
Cell Rep ; 34(11): 108871, 2021 03 16.
Article in English | MEDLINE | ID: mdl-33730583

ABSTRACT

The formation and consolidation of memories are complex phenomena involving synaptic plasticity, microcircuit reorganization, and the formation of multiple representations within distinct circuits. To gain insight into the structural aspects of memory consolidation, we focus on the calyx of the Drosophila mushroom body. In this essential center, essential for olfactory learning, second- and third-order neurons connect through large synaptic microglomeruli, which we dissect at the electron microscopy level. Focusing on microglomeruli that respond to a specific odor, we reveal that appetitive long-term memory results in increased numbers of precisely those functional microglomeruli responding to the conditioned odor. Hindering memory consolidation by non-coincident presentation of odor and reward, by blocking protein synthesis, or by including memory mutants suppress these structural changes, revealing their tight correlation with the process of memory consolidation. Thus, olfactory long-term memory is associated with input-specific structural modifications in a high-order center of the fly brain.


Subject(s)
Drosophila melanogaster/physiology , Memory Consolidation/physiology , Mushroom Bodies/innervation , Nerve Net/physiology , Animals , Axons/drug effects , Axons/physiology , Drosophila melanogaster/drug effects , Drosophila melanogaster/ultrastructure , Memory Consolidation/drug effects , Memory, Long-Term/drug effects , Mushroom Bodies/drug effects , Mushroom Bodies/ultrastructure , Nerve Net/drug effects , Nerve Net/ultrastructure , Neuronal Plasticity/drug effects , Odorants , Oleic Acids/pharmacology , Pheromones/pharmacology , Synapses/drug effects , Synapses/physiology , Synapses/ultrastructure
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