ABSTRACT
Conosiligins A-D (1-4), four ring-rearranged sesquiterpenoids, were isolated from cultures of the basidiomycete Conocybe siliginea. Their structures and absolute configurations were determined by detailed spectroscopic analyses and equivalent circulating density (ECD) calculations. Compounds 1 and 2 possess a 5/8-fused ring system, while 3 has a 5/6-fused backbone conjugated with a γ-lactone. Compound 4 is a 5,6-seco tremulane derivative with the loss of a skeletal carbon, featuring a tetracyclic system involving a pyranone moiety. Compounds 3 and 4 inhibited Con A-induced T cell proliferation with IC50 values of 12.3 and 6.6 µM, respectively.
Subject(s)
Agaricales/chemistry , Immunosuppressive Agents/chemistry , Immunosuppressive Agents/pharmacology , Sesquiterpenes/chemistry , Animals , B-Lymphocytes/drug effects , Cell Proliferation/drug effects , Cell Survival/drug effects , Female , Mice , Mice, Inbred BALB C , Molecular Structure , T-Lymphocytes/drug effectsABSTRACT
Three newly isolated ergosterols, psathergosterols A-C (1-3), together with two known ones (4 and 5), have been isolated from cultures of the basdiomycete Psathyrella candolleana. Their structures with the absolute configuration were elucidated by means of spectroscopic methods and the single crystal X-ray diffraction. Compounds 2-4 exhibited certain cytotoxicities to five human cancer cell lines (HL-60, SMMC-7721, A-549, MCF-7, SW480).
Subject(s)
Antineoplastic Agents/pharmacology , Basidiomycota/chemistry , Ergosterol/pharmacology , Antineoplastic Agents/isolation & purification , Cell Line, Tumor , China , Drug Screening Assays, Antitumor , Ergosterol/isolation & purification , Humans , Molecular StructureABSTRACT
Two highly oxygenated ergosterols, (22E,24R)-3ß,5α,6ß,9α,14α,25-hexhydroxyergosta-7,22-diene (1), and (22E,24R)-3ß,5α,6ß,14α,25-pentahydroxyergosta-7,9(11),22-triene (2), together with two known ones, have been isolated from cultures of the basidiomycete Conocybe siliginea. Their structures were elucidated on the basis of extensive spectroscopic means. Two new compounds (1 and 2) were tested for their nitric oxide synthase inhibitory activities. However, none of them showed any activity (IC50 > 40 µM).