Heat shock protein gene expression profile may differentiate between rheumatoid arthritis, osteoarthritis, and healthy controls.

OBJECTIVE: Heat shock proteins (Hsps) have been repeatedly implicated in the pathogenesis of rheumatoid arthritis (RA). The aim of this work was to study Hsp mRNA and protein levels to determine whether they can be used to differentiate between RA, osteoarthritis (OA), and healthy controls. METHODS: Hsp27, Hsp60, Hsp70, Hsp90α, and HspBP1 mRNA expression was analysed using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) in 24 RA, 11 OA, and 21 healthy controls. Hsp70 and HspBP1 protein levels were measured in serum using an enzyme-linked immunosorbent assay (ELISA). RESULTS: Hsp gene expression profiles differ significantly between inflammatory (RA) and non-inflammatory (OA) joint diseases, showing significantly increased Hsp27 and Hsp90α mRNA levels in RA synovial tissues. Up-regulated Hsp60 and Hsp90α together with down-regulated Hsp70 and elevated HspBP1/Hsp70 mRNA ratios can be used to differentiate between RA patients and healthy individuals through analysis of peripheral blood samples. Despite increased HspBP1 levels in RA sera, Hsp70 levels and the HspBP1/Hsp70 protein ratio remained identical in the RA patients and healthy individuals, which may contribute to the inhibition of Hsp70 anti-apoptotic activity. CONCLUSION: Hsp gene expression analysis can be implemented as a new diagnostic approach to facilitate differentiation between RA, OA, and healthy controls.

Isolation and characterization of isoforms of HspBP1, inhibitors of Hsp70.

The protein sequences derived from cDNA sequences for Hsp70 binding proteins from human (HspBP2) and rat tissues (HspBPR) are presented in this paper. The derived amino acid sequences of these proteins differ from human HspBP1 in the number of consecutive glycines near the amino-terminus. These differences, however, do not alter the inhibitory activity.

Synthesis of heat stress proteins in lymphocytes from livestock.

Cultured bovine, equine, ovine and chicken lymphocytes responded to heat stress by the increased synthesis of a specific set of proteins known as heat stress proteins (HSP). Proteins with molecular weights of 70 and 90 kDa were synthesized in all species. Additional proteins were found in bovine, ovine and chicken lymphocytes. A time course of induction showed an increased synthesis of some of these proteins with only 30 min of heat stress and of several proteins with 60 min of heat stress. A specific monoclonal antibody was used to identify HSP70 as one of the stress proteins in bovine lymphocytes. Cells from these animals are capable of responding to heat stress by the production of heat stress proteins. These may be of physiological importance.

Inhibition of Hsp70 ATPase activity and protein renaturation by a novel Hsp70-binding protein.

A cDNA that codes for an Hsp70-interacting protein (HspBP1) was isolated from a human heart cDNA library using the yeast two-hybrid system. The derived amino acid sequence is unique and therefore represents a new regulator of Hsp70. Northern blots of RNA from human tissues indicate that HspBP1 mRNA has a size of approximately 1.7 kilobase pairs and is present in all tissues analyzed but is most abundant in heart and skeletal muscle. Western blot analysis revealed a protein of approximately 40 kilodaltons detected in cell extracts. The ATPase domain of Hsp70 demonstrated binding to HspBP1. Further experiments showed binding of HspBP1 to Hsp70 and Hsc70 in a total heart extract. HspBP1 (8 microM) inhibited approximately 90% of the Hsp40-activated Hsp70 ATPase activity. HspBP1 prevented ATP binding to Hsp70, and therefore this is the likely mechanism of inhibition. Hsp40-activated ATPase activity is essential for the renaturation activity of Hsp70; therefore, the effects of HspBP1 on renaturation of luciferase in a reticulocyte lysate and a defined system were examined. HspBP1 inhibited renaturation with half-maximal inhibition at 2 microM. These data indicate that we have identified a novel Hsp70-interacting protein that inhibits Hsp70 chaperone activity.

Photosynthesis and Activity of Ribulose Bisphosphate Carboxylase of Wheat and Maize Seedlings during and following Exposure to O(2)-Low, CO(2)-Free N(2).

Seven day old wheat and maize seedlings were exposed to 1300 or 2000 microeinsteins per square meter per second photosynthetically active radiation in CO(2)-free air for 3 hours with either 1% O(2) in N(2) or N(2)-only and then returned to normal air of 340 microliters per liter CO(2), 21% O(2) in N(2). Activity of the ribulose bisphosphate carboxylase and amount of the substrate, ribulose 1,5-bisphosphate, were measured during and following the CO(2)-free treatments as was photosynthetic CO(2) fixation. Photoinhibition of photosynthesis was observed only with wheat seedlings following the N(2) only treatment. During the CO(2)-free treatments, the levels of RuBP rose during all experiments except when wheat was photoinhibited. The activity of the ribulose bisphophate carboxylase, measured directly upon grinding the leaves, declined during the CO(2)-free conditions. The carboxylase total activity increased in minutes in the leaf during and following the CO(2)-free treatments. The specific activities of the wheat carboxylase went from 0.16 to 1.06 micromoles CO(2) fixed per milligram protein per minute while the maize carboxylase varied from 0.05 to 0.36 micromole CO(2) fixed per millogram protein per minute. This suggests that in these seedlings considerable inactive carboxylase must be stored in a form not activatable in extracts by CO(2) and Mg(2+). Possible mechanisms of regulation of photosynthesis by the ribulose bisphosphate carboxylase must consider not only the amount of active enzyme, but the amount of enzyme which the plant can make activatable upon demand.

Measurement and preservation of the in vivo activation of ribulose 1,5-bisphosphate carboxylase in leaf extracts.

Photosynthetic carbon fixation is regulated in the chloroplast by the amount of ribulose 1,5-bisphosphate carboxylase which is activated. The activated carboxylase was preserved in detached leaves (barley, maize, soybean, spinach, wheat) for 90 min when stored on ice. With leaf extracts stored at 2 degrees C, the amount of activated enzyme, representing that originally in the leaf, as well as the fully activated enzyme, formed by incubation of leaf extracts with Mg(2+) and bicarbonate, both slowly declined in activity. However, for each activity this decline was proportional such that the ratio (percent activation) appeared constant. No change was observed in activation of the enzyme during the brief time of leaf homogenization. Optimal conditions (Mg(2+), incubation time) for measurement of leaf activation of ribulose bisphosphate carboxylase vary depending on the plant.3-Phosphonoproprionate, a positive effector of the purified ribulose bisphosphate carboxylase and a metabolically inert analog of 2-phosphoglycolate, was used to examine what metabolic effectors might do to enzyme activation during leaf homogenization and preparation of the extract at 2 degrees C. Activation under these conditions was not altered by 3-phosphonoproprionate. When 3-phosphonoproprionate was brushed on attached leaves or taken up by the transpiration stream of detached leaves, a considerable increase in activation of the carboxylase was measured.

Light limitation of photosynthesis and activation of ribulose bisphosphate carboxylase in wheat seedlings.

In limiting light the activation of ribulose-1,5-bisphosphate (RuP(2)) carboxylase [3-phospho-D-glycerate carboxylyase (dimerizing), EC 4.1.1.39] in leaf extracts of 7- to 8-day-old wheat seedlings changed proportionally with the photosynthetic rate of the intact plants. Higher rates of photosynthesis, induced by increasing irradiances, were accompanied by an increase in activation of the leaf RuP(2) carboxylase, while RuP(2) levels remained unchanged. The degree of activation varied from 20% to 60% of full activation at irradiances of 225-1650 muE/m(2).s (photosynthetically active radiation; E = einstein, 1 mol of photons). Between 225 muE/m(2).s and darkness, activation approached 50% while RuP(2) levels dropped more than 90%. During steady-state photosynthesis, levels of the substrate RuP(2) were 250-300 nmol/mg of chlorophyll in the leaves and were similar at all irradiances above 225 muE/m(2).s (25% of light saturation). When velocities of the carboxylase in leaf extracts were corrected for CO(2) levels estimated to exist within the leaf, they compared favorably with the photosynthetic rates of the intact seedlings. Comparison of CO(2) exchange rate, RuP(2) level, and activation of the carboxylase indicates that light limitation of photosynthesis can be due to two factors: the availability of RuP(2) in dark to dim light and activation of the RuP(2) carboxylase in dim light and higher irradiances.

Sequence and expression of a baculovirus protein with antigenic similarity to telokin.

A protein from baculovirus-infected cells reacted with an antibody against the smooth muscle protein telokin. Because of this unusual similarity, the protein, termed telokin-like protein-20 (TLP20), was isolated and characterized. Its M(r) on denaturing polyacrylamide gels was 28K and the protein contained a high proportion of beta structure. A cDNA for TLP20 was isolated and sequenced. The 3' non-coding sequence contained a region of high identity with the 5' end of two other baculovirus genes. The 5' non-coding region contains several baculovirus regulatory elements. Surprisingly, the derived amino acid sequence showed no homologies to telokin. The cDNA was cloned into a bacterial expression vector and the subsequently expressed protein had a slightly lower M(r) than the native protein, but cross-reacted with telokin antibody. This paper reports the characterization of a new baculovirus protein that shares some antigenic similarities to the smooth muscle protein telokin.

HSP70-related proteins in bovine skeletal muscle.

Constitutive expression of HSP70-related proteins was detected in a variety of bovine tissues using a specific antibody. All tissues contained a 73 kilodalton protein. A lower molecular weight form (72 kilodaltons) that co-migrated on two-dimensional gels with the stressed-induced HSP70 was present in high levels in bovine skeletal muscle, but absent from rat skeletal muscle. Two-dimensional gel analysis revealed several isoforms for both the 73 and 72 kilodalton forms. Purification of HSP70-related proteins from bovine skeletal muscle, thymus gland and rat skeletal muscle demonstrated that the antibody recognized all the forms present in the tissue homogenates. The two proteins are similar but distinct as detected by one-dimensional peptide mapping. The lower molecular form was not present in fetal tissue but was detectable in newborn animals, suggesting that the levels are regulated during development.

Molecular structure of a proteolytic fragment of TLP20.

Myosin light-chain kinase is responsible for the phosphorylation of myosin in smooth muscle cells. In some tissue types, the C-terminal portion of this large enzyme is expressed as an independent protein and has been given the name telokin. Recently, an antibody directed against telokin was found to interact with a protein derived from the baculovirus Autographa californica nuclear polyhedrosis virus. This protein was biochemically characterized and given the name TLP20 for telokin-like protein of 20 000 molecular weight. The amino-acid sequence of TLP20 was determined on the basis of a cDNA clone and subsequent alignment searches failed to reveal any homology to telokin or to other known proteins. The three-dimensional structure of a proteolytic portion of TLP20 is reported here. Crystals employed in the investigation were grown from ammonium sulfate solutions at pH 6.0 and belonged to the space group P2(1)3 with unit-cell dimensions of a = b = c = 76.3 A and one molecule per asymmetric unit. The structure was determined by multiple isomorphous replacement with three heavy-atom derivatives. Least-squares refinement of the model reduced the crystallographic R factor to 18.1% for all measured X-ray data from 30.0 to 2.2 A. The overall fold of the molecule may be described as a seven-stranded antiparallel beta-barrel flanked on the bottom by two additional beta-strands and on the top by an alpha-helix. Quite surprisingly, the three-dimensional structure of this beta-barrel is not similar to telokin or to any other known protein.