ABSTRACT
A novel actinomycete, designated strain OU-40(T), was isolated from farm soil collected from the Hyderabad region of Andhra Pradesh, southern India. The strain was found to have morphological and chemotaxonomic characteristics typical of species of the genus Streptomyces. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain OU-40(T) belonged to the genus Streptomyces, and was related most closely to Streptomyces pactum NBRC 13433(T) (99.0â% sequence similarity), Streptomyces olivaceus NBRC 12805(T) (99.0â%) and Streptomyces parvulus NBRC 13193(T) (98.8â%). Strain OU-40(T) could be distinguished from the type strains of its closest phylogenetic relatives based on levels of DNA-DNA relatedness and comparison of morphological and phenotypic data. It is therefore concluded that strain OU-40(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces hyderabadensis sp. nov. is proposed. The type strain is OU-40(T) (=CCTCC AA 209024(T) =PCM 2692(T)).
Subject(s)
Soil Microbiology , Streptomyces/classification , Streptomyces/isolation & purification , Bacterial Typing Techniques , Cluster Analysis , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , India , Molecular Sequence Data , Nucleic Acid Hybridization , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Streptomyces/genetics , Streptomyces/physiologyABSTRACT
We report the single-contig genome sequence of the anaerobic, mesophilic, cellulolytic bacterium, Bacteroides cellulosolvens. The bacterium produces a particularly elaborate cellulosome system, wherein the types of cohesin-dockerin interactions are opposite of other known cellulosome systems: cell-surface attachment is thus mediated via type-I interactions, whereas enzymes are integrated via type-II interactions.
ABSTRACT
Four Ru(II) polypyridyl complexes, [Ru(bpy)(2)(7-NO(2)-dppz)](2+), [Ru(bpy)(2)(7-CH(3)-dppz)](2+), [Ru(phen)(2)(7-NO(2)-dppz)](2+), and [Ru(phen)(2)(7-CH(3)-dppz)](2+) (bpy = 2,2'-bipyridine, phen = 1,10-phenanthroline), (7-Nitro-dppz = 7-Nitro dipyrido[3,2-a:2'-3'-c]phenazine, 7-CH(3)-dppz = 7-Methyl dipyrido[3,2-a:2'-3'-c]phenazine), have been synthesized and characterized by IR, UV, elemental analysis, (1)H NMR, (13)C-NMR, and mass spectroscopy. The DNA-binding properties of the four complexes were investigated by spectroscopic and viscosity measurements. The results suggest that all four complexes bind to DNA via an intercalative mode. Under irradiation at 365 nm, all four complexes were found to promote the photocleavage of plasmid pBR 322 DNA. Toxicological effects of the selected complexes were performed on industrially important yeasts (eukaryotic microorganisms).
Subject(s)
Candida/drug effects , DNA/chemistry , Organometallic Compounds/chemistry , Organometallic Compounds/toxicity , Ruthenium/chemistry , Saccharomyces cerevisiae/drug effects , Binding Sites , Candida/genetics , DNA Cleavage , Organometallic Compounds/chemical synthesis , Photochemistry , Saccharomyces cerevisiae/genetics , Spectrometry, Fluorescence , Spectrophotometry, Ultraviolet , ViscosityABSTRACT
The polypyridyl ligand 7-Nitro dipyrido[3,2-a:2'-3'-c]phenazine (7-Nitro-dppz) and its complexes [Co(bpy)(2)(7-NO(2)-dppz)](3+)(1) (bpy = 2,2'-bipyridine), [Co(dmb)(2)(7-NO(2)-dppz)](3+)(2), (dmb = 4,4'-dimethyl-2,2'-bipyridine), and [Co(phen)(2)(7-NO(2)-dppz)](3+)(3) (phen = 1,10-phenanthroline) were synthesized and characterized by UV/VIS, IR, elemental analysis, (1)H and (13)C-NMR, and mass spectra. The binding properties of the three complexes to CT-DNA were investigated by different spectroscopic methods and viscosity measurements and DNA cleavage assay. The experimental results suggest that these complexes bind to CT-DNA through an intercalative mode. Also, the three complexes promote the photocleavage of plasmid pBR-322 DNA under irradiation. Toxicological effects of the selected complexes were estimated with different microorganisms.
Subject(s)
2,2'-Dipyridyl/chemistry , Cobalt , DNA Cleavage , DNA , Phenanthrolines/chemistry , Photochemistry , Pyridines/chemistry , Animals , Bacteria/drug effects , Cobalt/chemistry , Cobalt/pharmacology , DNA/chemistry , DNA/metabolism , Fungi/drug effects , Intercalating Agents/chemistry , Microbial Sensitivity Tests , Molecular Structure , Nucleic Acid Denaturation , Plasmids/genetics , Plasmids/metabolism , ViscosityABSTRACT
Five of the 207 isolates from different composts, farm waste compost (FWC), rice straw compost (RSC), Gliricidia vermicompost (GVC), and macrofauna, showed rock phosphate (RP) solubilization in buffered medium in plate culture. When tested in RP broth medium, all five strains, Enterobacter cloacae EB 27, Serratia marcescens EB 67, Serratia sp. EB 75, Pseudomonas sp. CDB 35, and Pseudomonas sp. BWB 21, showed gluconic acid production and solubilized RP. Based on cellulose-degrading and P-solubilizing ability, two strains were selected for further studies. In the presence of different carbon sources, both strains showed a drop in pH and solubilized RP. P released was maximum with glucose (1212 and 522 micromol) and minimum with cellobiose (455 and 306 micromol) by S. marcescens EB 67 and Pseudomonas sp. CDB 35, respectively. Glucose dehydrogenase (GDH) activity was 63 and 77% with galactose and 35 and 46% with cellobiose when compared to glucose (100%) by EB 67 and CDB 35, respectively. Both strains solubilized RP in the presence of different crop residues. EB 67 and CDB 35 showed maximum cellulase activity (0.027 units) in the presence of rice straw and a mixture of rice straw and root. P solubilized from RP in the presence of pigeonpea root was 134 and 140 micromol with EB 67 and CDB 35. Significantly, these bacteria isolated from composts and macrofauna solubilized rock phosphate in the presence of various pure carbon substrates and crop residues and their importance in soil/rhizosphere conditions is discussed.