The interactive effects of verapamil and CB1 cannabinoid receptor antagonist/inverse agonist, AM251 on passive avoidance learning and memory in rat.

There are reports regarding the effects of intracellular Ca2+ and synthesis and release of endocannabinoids. The secretion of endocannabinoids depends on the L-type calcium channel. The present study evaluated the involvement of the cannabinoid CB1 receptors in the effect of L-type calcium channel blocker verapamil on passive avoidance learning (PAL) in adult male rats. In this study, we examined the effects of an acute administration of the cannabinoid CB1 receptors antagonist/inverse agonist AM251 following a chronic administration of the Ca2+ channel blocker verapamil on PAL. Male Wistar rats were administered verapamil (10, 25 and 50 mg/kg) or saline intraperitoneally (i.p) daily for 13 days (n = 10/group). After this treatment period, a learning test (acquisition) was performed, and a retrieval test was performed the following day. The results indicated that chronic systemic administration of verapamil (in a dose-dependent manner) impaired memory acquisition and retrieval. Pre-training acute administration of a selective CB1 antagonist/inverse agonist, AM251 (5 mg/kg, i.p.) did not change memory acquisition and retrieval. Co-administration of the verapamil and AM251 significantly reversed verapamil-induced amnesia, suggesting a functional interaction between AM251 and verapamil. The results indicated the interactive effects of cannabinoid CB1 receptors and L-type calcium channel in passive avoidance learning and AM251 can counter the effects of verapamil on memory.

Microplastics removal efficiency and risk analysis of wastewater treatment plants in Oman.

Microplastics (MPs) have recently been documented as an emerging pollutant that poses a critical threat to environment. Wastewater treatment plants (WWTPs) are commonly regarded as significant contributors to the presence of MPs. This study aimed to assess the MPs load of three wastewater treatment facilities in Oman using various treatments, including MBR, SBR, and CAS. Wastewater samples from influent, effluent, and sludge were collected and analyzed to determine the concentration, morphology, size, color, and polymer type of the MPs. A set of sieves with a mesh size range of 1 mm-45 µm was used to for filtration. Oxidation treatment was applied for all samples using Fenton's reagent, followed by density separation by sodium chloride solution. The Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR- FTIR) method was utilized to test 10% from each sampling point to confirm the polymer types of the MPs. The pollution load index (PLI) and hazard index (HI) have been employed to assess the risk associated with the chemical toxicity and concentration of detected particles. The PROMETHEE method was used to rank the risk of sampling sites based on different criteria that posed potential ecological and human health risks. The results indicate that the average concentrations of 0.99 MP/L, 1.38 MP/L, and 0.93 MP/L were detected in the final treated effluent of WWTP A, WWTP B, and WWTP C, respectively. These concentrations correspond to overall removal efficiencies of 82.5%, 77.4%, and 79.2% for WWTP A, WWTP B, and WWTP C, respectively Most MPs found in tertiary effluent were smaller particles (425 µm) and fiber-shaped. The major types of MPs were polypropylene (PP), low-density polyethylene (LDPE), polyurethane (PU), polyethylene terephthalate (PET), and Polyvinyl chloride (PVC). This study showed that treated effluent and sludge release significant MPs into the environment.

Environmental exposure and respiratory health: Unraveling the impact of toxic release inventory facilities on COPD prevalence.

This cross-sectional geospatial analysis explores the prevalence of Chronic Obstructive Pulmonary Disease (COPD) concerning the proximity to toxic release inventory (TRI) facilities in Jefferson County, Alabama. Employing the fuzzy analytical hierarchy process (FAHP), the study evaluates COPD prevalence, comorbidities, healthcare access, and individual health assessments. Given the mounting evidence linking environmental pollutants to COPD exacerbations, the research probes the influence of TRI sites on respiratory health, integrating Geographic Information Systems (GIS) to scrutinize the geospatial vulnerability of communities neighboring TRI sites. Socio-demographic disparities, economic conditions, and air pollution are emphasized in the analysis. The EPA's Toxic Release Inventory serves as the cornerstone for assessing the association between TRI proximity and COPD prevalence. The analysis uncovers a notable inverse correlation between distance from TRI sites and COPD prevalence, signaling potential health risks for populations residing closer to these facilities. Moreover, factors such as minority status, low income, and air pollution are associated with higher COPD prevalence, underscoring the imperative of comprehending the interplay between environmental exposure and respiratory health. This study bridges gaps in the literature by addressing the geographical nexus between COPD prevalence and pollution exposure. By leveraging FAHP, the research furnishes a holistic understanding of the multifaceted factors influencing vulnerability to COPD. The findings underscore the necessity for targeted public health interventions and policy measures to redress environmental disparities and alleviate the repercussions of TRI facilities on respiratory health.