ABSTRACT
Ketenimines are versatile synthetic intermediates capable of performing novel transformations in organic synthesis. They are normally generated in situ due to their inherent instability and high level of reactivity. Herein, we report pyridine-stabilized ketenimine zwitterionic salts, which are prepared through click chemistry from readily accessible alkynes and sulfonyl azides. To demonstrate their synonymous reactivity to ketenimines, these salts have been utilized in a cascade sequence to access highly functionalized quinolines including the core structures of an antiprotozoal agent and the potent topoisomerase inhibitor Tas-103.
ABSTRACT
Among the opioid receptors, the κ-opioid receptor (κOR) has been gaining considerable attention as a potential therapeutic target for the treatment of complex CNS disorders including depression, visceral pain, and cocaine addiction. With an interest in discovering novel ligands targeting κOR, we searched natural products for unusual scaffolds and identified collybolide (Colly), a nonnitrogenous sesquiterpene from the mushroom Collybia maculata. This compound has a furyl-δ-lactone core similar to that of Salvinorin A (Sal A), another natural product from the plant Salvia divinorum Characterization of the molecular pharmacological properties reveals that Colly, like Sal A, is a highly potent and selective κOR agonist. However, the two compounds differ in certain signaling and behavioral properties. Colly exhibits 10- to 50-fold higher potency in activating the mitogen-activated protein kinase pathway compared with Sal A. Taken with the fact that the two compounds are equipotent for inhibiting adenylyl cyclase activity, these results suggest that Colly behaves as a biased agonist of κOR. Behavioral studies also support the biased agonistic activity of Colly in that it exhibits â¼10-fold higher potency in blocking non-histamine-mediated itch compared with Sal A, and this difference is not seen in pain attenuation by these two compounds. These results represent a rare example of functional selectivity by two natural products that act on the same receptor. The biased agonistic activity, along with an easily modifiable structure compared with Sal A, makes Colly an ideal candidate for the development of novel therapeutics targeting κOR with reduced side effects.
Subject(s)
Agaricales/chemistry , Antipruritics/pharmacology , Diterpenes, Clerodane/pharmacology , MAP Kinase Signaling System/drug effects , Receptors, Opioid, kappa/agonists , Second Messenger Systems/drug effects , Animals , Antipruritics/chemistry , Diterpenes, Clerodane/chemistry , HEK293 Cells , Humans , Mice , Mice, Knockout , Receptors, Opioid, kappa/genetics , Receptors, Opioid, kappa/metabolismABSTRACT
A convergent approach for the stereoselective synthesis of diverse spiroethers is described. The reaction involves stereoselective trapping of diazo-derived rhodium carbenoids with gold-activated alkynols for the installation of spiro cores. The reaction has proven general with a range of readily accessible homopropargylic alcohols and diazo carbonyls to provide functionalized spiroether cores of bioactive scaffolds such as spirobarbiturates, spirooxindoles, and pseurotin natural products.
ABSTRACT
A novel and highly efficient diazo-OH insertion/Conia-ene cascade reaction of readily available homopropargylic acids and alcohols with diazo carbonyl compounds is described. The cascade reaction involves a synergistic Rh/Ag/Au catalyst cocktail and proceeds instantly with a variety of substituted diazo compounds and acids/alcohols to provide functionalized γ-butyrolactones and tetrahydrofurans with complete regio- and stereoselectivity. The unprecedented rate-enhancement, complete stereoselectivity, and the enabling of new Conia-ene cyclizations suggest a concerted [4+1]-cycloaddition reaction pathway under synergistic (Rh/Ag/Au)-catalysis conditions.
ABSTRACT
MenE is an o-succinylbenzoyl-CoA (OSB-CoA) synthetase in the bacterial menaquinone biosynthesis pathway and is a promising target for the development of novel antibacterial agents. The enzyme catalyzes CoA ligation via an acyl-adenylate intermediate, and we have previously reported tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogues of this intermediate, including OSB-AMS (1), which has an IC50 value of ≤25 nM for Escherichia coli MenE. Herein, we show that OSB-AMS reduces menaquinone levels in Staphylococcus aureus, consistent with its proposed mechanism of action, despite the observation that the antibacterial activity of OSB-AMS is â¼1000-fold lower than the IC50 for enzyme inhibition. To inform the synthesis of MenE inhibitors with improved antibacterial activity, we have undertaken a structure-activity relationship (SAR) study stimulated by the knowledge that OSB-AMS can adopt two isomeric forms in which the OSB side chain exists either as an open-chain keto acid or a cyclic lactol. These studies revealed that negatively charged analogues of the keto acid form bind, while neutral analogues do not, consistent with the hypothesis that the negatively charged keto acid form of OSB-AMS is the active isomer. X-ray crystallography and site-directed mutagenesis confirm the importance of a conserved arginine for binding the OSB carboxylate. Although most lactol isomers tested were inactive, a novel difluoroindanediol inhibitor (11) with improved antibacterial activity was discovered, providing a pathway toward the development of optimized MenE inhibitors in the future.
Subject(s)
Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Escherichia coli Proteins/antagonists & inhibitors , Escherichia coli Proteins/chemistry , Succinate-CoA Ligases/antagonists & inhibitors , Succinate-CoA Ligases/chemistry , Amino Acid Sequence , Animals , Anti-Bacterial Agents/toxicity , Arginine/chemistry , Catalytic Domain/genetics , Chlorocebus aethiops , Conserved Sequence , Crystallography, X-Ray , Drug Discovery , Escherichia coli K12/enzymology , Escherichia coli K12/genetics , Escherichia coli Proteins/genetics , Microbial Sensitivity Tests , Models, Molecular , Molecular Sequence Data , Mutagenesis, Site-Directed , Phenylbutyrates/chemistry , Phenylbutyrates/pharmacology , Phenylbutyrates/toxicity , Protein Conformation , Sequence Homology, Amino Acid , Staphylococcus aureus/drug effects , Staphylococcus aureus/metabolism , Structure-Activity Relationship , Succinate-CoA Ligases/genetics , Vero Cells , Vitamin K 2/metabolismABSTRACT
MenE, the o-succinylbenzoate (OSB)-CoA synthetase from bacterial menaquinone biosynthesis, is a promising new antibacterial target. Sulfonyladenosine analogues of the cognate reaction intermediate, OSB-AMP, have been developed as inhibitors of the MenE enzymes from Mycobacterium tuberculosis (mtMenE), Staphylococcus aureus (saMenE) and Escherichia coli (ecMenE). Both a free carboxylate and a ketone moiety on the OSB side chain are required for potent inhibitory activity. OSB-AMS (4) is a competitive inhibitor of mtMenE with respect to ATP (K(i) =5.4±0.1 nM) and a noncompetitive inhibitor with respect to OSB (K(i) =11.2±0.9 nM). These data are consistent with a Bi Uni Uni Bi Ping-Pong kinetic mechanism for these enzymes. In addition, OSB-AMS inhibits saMenE with K(i)(app) =22±8 nM and ecMenE with K(i)(OSB) =128±5 nM. Putative active-site residues, Arg222, which may interact with the OSB aromatic carboxylate, and Ser302, which may bind the OSB ketone oxygen, have been identified through computational docking of OSB-AMP with the unliganded crystal structure of saMenE. A pH-dependent interconversion of the free keto acid and lactol forms of the inhibitors is also described, along with implications for inhibitor design.
Subject(s)
Adenosine Monophosphate/pharmacology , Enzyme Inhibitors/pharmacology , Phenylbutyrates/pharmacology , Succinate-CoA Ligases/antagonists & inhibitors , Vitamin K 2/metabolism , Adenosine Monophosphate/chemical synthesis , Adenosine Monophosphate/chemistry , Catalytic Domain/drug effects , Enzyme Inhibitors/chemical synthesis , Enzyme Inhibitors/chemistry , Escherichia coli/enzymology , Escherichia coli/metabolism , Models, Molecular , Molecular Structure , Mycobacterium tuberculosis/enzymology , Mycobacterium tuberculosis/metabolism , Phenylbutyrates/chemical synthesis , Phenylbutyrates/chemistry , Staphylococcus aureus/enzymology , Staphylococcus aureus/metabolism , Stereoisomerism , Structure-Activity Relationship , Succinate-CoA Ligases/metabolism , Vitamin K 2/chemistryABSTRACT
The genesis and development of the 4,6-O-benzylidene acetal method for the preparation of ß-mannopyranosides are reviewed. Particular emphasis is placed on the influence of the various protecting groups on stereoselectivity and these effects are interpreted in the framework of a general mechanistic scheme invoking a series of solvent-separated and contact ion pairs in dynamic equilibrium with a covalent α-glycosyl trifluoromethanesulfonate.
Subject(s)
Benzylidene Compounds/chemistry , Glycosylation , Mannose/chemistry , Electrons , Safrole/analogs & derivatives , Safrole/chemistry , Stereoisomerism , Thioglycosides/chemistryABSTRACT
Attachment of a growing peptide chain to a glycylaminomethyl resin via a thioglycinamide bond is compatible with Fmoc-chemistry solid-phase peptide synthesis. Subsequent S-alkylation of the thioamide gives a thioimide that, on treatment with aqueous trifluoroacetic acid, releases the peptide from the resin in the form of a C-terminal thioester.
Subject(s)
Fluorenes/chemistry , Fluorenes/chemical synthesis , Oligopeptides/chemical synthesis , Peptides/chemical synthesis , Thioamides/chemistry , Magnetic Resonance Spectroscopy , Molecular Sequence Data , Molecular Structure , Oligopeptides/chemistry , Peptides/chemistry , Sulfhydryl Compounds/chemical synthesis , Sulfhydryl Compounds/chemistryABSTRACT
α-C-Glucopyranosides and mannopyranosides are obtained in 65-85% yields from 4,6-O-benzylidene-protected glucosyl and mannosyl thioglycosides bearing ester functionality at the 3-O-position by a coupling reaction with C-nucleophiles on activation with diphenyl sulfoxide, 2,4,6-tri-tert-butylpyrimidine, and trifluoromethanesulfonic anhydride.
Subject(s)
Benzylidene Compounds/chemistry , Indicators and Reagents/chemistry , Mannose/chemistry , Oxides/chemistry , Thioglycosides/chemistry , Esters , Glycosylation , Magnetic Resonance Spectroscopy , Molecular Structure , StereoisomerismABSTRACT
A metal-free aromative cascade has been developed for the synthesis of diverse heterocycles from readily accessible hydroxy/aminochalcones and acid/alkyl halides. The cascade being by a base-mediated intramolecular aldol cyclization/dehydration sequence to provide a triene, which sets the stage for a 6π-electrocyclization/oxidative aromatization to access diverse heterocyclic scaffolds.
ABSTRACT
Highly activated thioesters formed by the rapid reaction of C-terminal thioacids derived from protected amino acids and peptides with the Sanger reagent and other electron-deficient aryl halides in the presence of a free amine immediately form a peptide bond with the amine. This essentially epimerization-free method was used for the 4+4 block synthesis of a hindered octapeptide (see scheme; Boc, Pbf, and Trt are protecting groups).
Subject(s)
Amines/chemistry , Peptides/chemical synthesis , Sulfhydryl Compounds/chemistry , Amino Acid Sequence , Amino Acids/chemistry , Peptides/chemistry , Sulfur Compounds/chemistryABSTRACT
A convergent cascade approach for the stereoselective synthesis of diverse lactones is described. The Rh2(TFA)4-catalyzed cascade reaction proceeds via a carboxylic acid O-H insertion/aldol cyclization with high chemo-, regio-, and diastereoselectivity. The cascade reaction provides quick access to highly functionalized γ-butyro- and δ-valerolactones from readily accessible ketoacid and diazo synthons. To demonstrate the utility of this approach, a thermally induced oxy-Cope ring-expansion strategy has been incorporated in the cascade sequence to access medium-sized lactones, which can undergo a serendipitous rearrangement to form spiro-lactones through an intramolecular aldol/trans-lactonization sequence. The reaction has proven to be general, with a range of ketoacids and diazo carbonyls to provide functionalized lactones of varying ring sizes.
ABSTRACT
A serendipitous five-step cascade of rhodium vinylcarbenoids with aminochalcones enables a unique synthetic approach to highly functionalized tri- and tetra-cyclic quinolines. The cascade reaction begins with the insertion of aminochalcone nitrogen into rhodium vinylcarbenoids followed by intramolecular aldol cyclization to provide a substituted indoline intermediate that undergoes an oxy-Cope rearrangement to provide a 9-membered azacycle, which then rearranges to the functionalized quinoline through an intramolecular aldol/dehydration sequence. With a catalyst loading as low as 0.1 mol%, the cascade reaction has proven to be general with a range of aminochalcones and vinylcarbenoids.
ABSTRACT
A novel diazo-cascade approach has been developed for the synthesis of nine-membered oxacycles utilizing readily accessible ß-hydroxy vinyl ketones and vinyl diazo esters. The Rh(II)-catalyzed cascade reaction begins with carbene O-H insertion followed by an intramolecular aldol cyclization to provide a substituted tetrahydrofuran intermediate that undergoes an oxy-Cope rearrangement to provide functionalized nine-membered oxacycles with complete stereoselectivity.
ABSTRACT
The Gram-negative bacterial pathogen Pseudomonas aeruginosa uses three interconnected intercellular signaling systems regulated by the transcription factors LasR, RhlR, and MvfR (PqsR), which mediate bacterial cell-cell communication via small-molecule natural products and control the production of a variety of virulence factors. The MvfR system is activated by and controls the biosynthesis of the quinolone quorum sensing factors HHQ and PQS. A key step in the biosynthesis of these quinolones is catalyzed by the anthranilyl-CoA synthetase PqsA. To develop inhibitors of PqsA as novel potential antivirulence antibiotics, we report herein the design and synthesis of sulfonyladeonsine-based mimics of the anthranilyl-AMP reaction intermediate that is bound tightly by PqsA. Biochemical, microbiological, and pharmacological studies identified two potent PqsA inhibitors, anthranilyl-AMS (1) and anthranilyl-AMSN (2), that decreased HHQ and PQS production in P. aeruginosa strain PA14. However, these compounds did not inhibit production of the virulence factor pyocyanin. Moreover, they exhibited limited bacterial penetration in compound accumulation studies. This work provides the most potent PqsA inhibitors reported to date and sets the stage for future efforts to develop analogues with improved cellular activity to investigate further the complex relationships between quinolone biosynthesis and virulence factor production in P. aeruginosa and the therapeutic potential of targeting PqsA.
Subject(s)
Coenzyme A Ligases/antagonists & inhibitors , Enzyme Inhibitors/pharmacology , Pseudomonas aeruginosa/drug effects , Quinolones/metabolism , Small Molecule Libraries , Enzyme Inhibitors/chemistry , Pseudomonas aeruginosa/enzymology , Pseudomonas aeruginosa/metabolismSubject(s)
Acids , Esters , Peptides/chemistry , Peptides/chemical synthesis , Sulfonamides , Sulfur Compounds , Acids/chemical synthesis , Acids/chemistry , Amino Acid Sequence , Esters/chemical synthesis , Esters/chemistry , Molecular Sequence Data , Molecular Structure , Peptides/genetics , Sulfonamides/chemical synthesis , Sulfonamides/chemistry , Sulfur Compounds/chemical synthesis , Sulfur Compounds/chemistryABSTRACT
A stereocontrolled synthesis of benzannulated spiroketals has been developed using solvent-dependent Sc(OTf)3-mediated spirocyclizations of exo-glycal epoxides having alcohol side chains. In THF, the reaction proceeds via Lewis acid catalysis under kinetic control with inversion of configuration at the anomeric carbon. In contrast, in CH2Cl2, Brønsted acid catalysis under thermodynamic control leads to retention of configuration. The reactions accommodate a variety of aryl substituents and ring sizes and provide stereochemically diverse spiroketals.
Subject(s)
Epoxy Compounds/chemistry , Furans/chemical synthesis , Lewis Acids/chemistry , Mesylates/chemistry , Scandium/chemistry , Solvents/chemistry , Spiro Compounds/chemical synthesis , Furans/chemistry , Molecular Structure , Spiro Compounds/chemistry , StereoisomerismABSTRACT
The formation of an unexpected heterocyclic scaffold, a benzoxazole, in a three-component reaction between a ketone, isocyanide, and 2-aminophenol was encountered. This reaction involved a benzo[b][1,4]oxazine intermediate resulting from intramolecular attack of the aminophenol hydroxyl group on the nitrilium ion. Unlike previous literature examples, the trapped nitrilium benzo[b][1,4]oxazine could readily be subjected to ring opening with bis-nucleophiles. The reaction scope includes simple linear as well as complex cyclic ketones and substituted 2-aminophenols. A representative benzoxazole product could be further diversified to yield drug-like compounds.
Subject(s)
Aminophenols/chemistry , Benzoxazoles/chemical synthesis , Ketones/chemistry , Nitriles/chemical synthesis , Oxazines/chemistry , Benzoxazoles/chemistry , Molecular Structure , Nitriles/chemistry , StereoisomerismABSTRACT
It is reported that the replacement of the 4- and 6-O-benzyl ethers in 2,3,4,6-tetra-O-benzyl-α,ß-mannopyranose by a 4,6-O-benzylidene acetal results in an increased population of the ß-anomer at equilibrium in CDCl(3) solution. The phenomenon is considered to arise from the lower steric bulk of the benzylidene acetal that, through diminished buttressing interactions, reduces steric interactions normally present in the ß-anomer.