1.
Antimicrob Agents Chemother
; 65(7): e0250220, 2021 06 17.
Article
in English
| MEDLINE
| ID: mdl-33903113
ABSTRACT
Eis promoter mutations can confer reduced Mycobacterium tuberculosis kanamycin susceptibility. GenoType MTBDRsl, a widely used assay evaluating this region, wrongly classified 17/410 isolates as eis promoter wild type. Six out of seventeen isolates harbored mutations known to confer kanamycin resistance, and the remainder harbored either novel eis promoter mutations (7/11) or disputed mutations (4/11). GenoType MTBDRsl can miss established and new variants that cause reduced susceptibility. These data highlight the importance of reflex phenotypic kanamycin testing.
Subject(s)
Mycobacterium tuberculosis , Tuberculosis, Multidrug-Resistant , Antitubercular Agents/pharmacology , Antitubercular Agents/therapeutic use , Diagnostic Tests, Routine , Drug Resistance, Multiple, Bacterial , Genotype , Humans , Kanamycin/pharmacology , Kanamycin Resistance/genetics , Microbial Sensitivity Tests , Mutation/genetics , Mycobacterium tuberculosis/genetics , Tuberculosis, Multidrug-Resistant/diagnosis , Tuberculosis, Multidrug-Resistant/drug therapy , Tuberculosis, Multidrug-Resistant/genetics
2.
J Infect Dis
; 195(12): 1871-2; author reply 1872-3, 2007 Jun 15.
Article
in English
| MEDLINE
| ID: mdl-17492605