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1.
Immunity ; 47(2): 323-338.e6, 2017 08 15.
Article in English | MEDLINE | ID: mdl-28813661

ABSTRACT

Tumor-associated macrophages (TAMs) are essential components of the cancer microenvironment and play critical roles in the regulation of tumor progression. Optimal therapeutic intervention requires in-depth understanding of the sources that sustain macrophages in malignant tissues. In this study, we investigated the ontogeny of TAMs in murine pancreatic ductal adenocarcinoma (PDAC) models. We identified both inflammatory monocytes and tissue-resident macrophages as sources of TAMs. Unexpectedly, significant portions of pancreas-resident macrophages originated from embryonic development and expanded through in situ proliferation during tumor progression. Whereas monocyte-derived TAMs played more potent roles in antigen presentation, embryonically derived TAMs exhibited a pro-fibrotic transcriptional profile, indicative of their role in producing and remodeling molecules in the extracellular matrix. Collectively, these findings uncover the heterogeneity of TAM origin and functions and could provide therapeutic insight for PDAC treatment.


Subject(s)
Carcinogenesis , Carcinoma, Ductal/immunology , Macrophages/immunology , Pancreas/pathology , Pancreatic Neoplasms/immunology , Animals , Carcinoma, Ductal/pathology , Cell Differentiation , Cell Line, Tumor , Cell Movement , Extracellular Matrix/metabolism , Fetal Development , Fibrosis , Hematopoiesis , Macrophages/pathology , Mice , Mice, Inbred C57BL , Mice, Knockout , Monocytes/immunology , Pancreatic Neoplasms/pathology , Tumor Microenvironment
2.
Immunity ; 40(1): 91-104, 2014 Jan 16.
Article in English | MEDLINE | ID: mdl-24439267

ABSTRACT

Cardiac macrophages are crucial for tissue repair after cardiac injury but are not well characterized. Here we identify four populations of cardiac macrophages. At steady state, resident macrophages were primarily maintained through local proliferation. However, after macrophage depletion or during cardiac inflammation, Ly6c(hi) monocytes contributed to all four macrophage populations, whereas resident macrophages also expanded numerically through proliferation. Genetic fate mapping revealed that yolk-sac and fetal monocyte progenitors gave rise to the majority of cardiac macrophages, and the heart was among a minority of organs in which substantial numbers of yolk-sac macrophages persisted in adulthood. CCR2 expression and dependence distinguished cardiac macrophages of adult monocyte versus embryonic origin. Transcriptional and functional data revealed that monocyte-derived macrophages coordinate cardiac inflammation, while playing redundant but lesser roles in antigen sampling and efferocytosis. These data highlight the presence of multiple cardiac macrophage subsets, with different functions, origins, and strategies to regulate compartment size.


Subject(s)
Macrophages/immunology , Monocytes/physiology , Myocarditis/immunology , Myocardium/immunology , Animals , Antigen Presentation , Antigens, Ly/metabolism , Cell Death , Cell Differentiation , Cell Lineage , Cells, Cultured , Fetal Development , Heart/embryology , Homeostasis , Mice , Mice, Inbred C57BL , Mice, Transgenic , Myocytes, Cardiac/immunology , Phagocytosis , Receptors, CCR2/metabolism , Transcriptome , Yolk Sac/cytology
3.
Immunity ; 39(3): 599-610, 2013 Sep 19.
Article in English | MEDLINE | ID: mdl-24012416

ABSTRACT

It is thought that monocytes rapidly differentiate to macrophages or dendritic cells (DCs) upon leaving blood. Here we have shown that Ly-6C⁺ monocytes constitutively trafficked into skin, lung, and lymph nodes (LNs). Entry was unaffected in gnotobiotic mice. Monocytes in resting lung and LN had similar gene expression profiles to blood monocytes but elevated transcripts of a limited number of genes including cyclo-oxygenase-2 (COX-2) and major histocompatibility complex class II (MHCII), induced by monocyte interaction with endothelium. Parabiosis, bromodoxyuridine (BrdU) pulse-chase analysis, and intranasal instillation of tracers indicated that instead of contributing to resident macrophages in the lung, recruited endogenous monocytes acquired antigen for carriage to draining LNs, a function redundant with DCs though differentiation to DCs did not occur. Thus, monocytes can enter steady-state nonlymphoid organs and recirculate to LNs without differentiation to macrophages or DCs, revising a long-held view that monocytes become tissue-resident macrophages by default.


Subject(s)
Cell Differentiation , Dendritic Cells/metabolism , Lymph Nodes/cytology , Macrophages/metabolism , Monocytes/immunology , Monocytes/metabolism , Animals , Antigens, Ly/metabolism , Cell Movement , Cyclooxygenase 2/genetics , Dendritic Cells/cytology , Dendritic Cells/immunology , Endothelium/metabolism , Histocompatibility Antigens Class II/genetics , Histocompatibility Antigens Class II/immunology , Lung/cytology , Lymph Nodes/immunology , Macrophages/cytology , Macrophages/immunology , Mice , Mice, Inbred C57BL , Skin/cytology
5.
J Immunol ; 201(9): 2551-2556, 2018 11 01.
Article in English | MEDLINE | ID: mdl-30275046

ABSTRACT

NK cells accumulate in adult murine and human uteri during decidualization induced physiologically, pathologically, or experimentally. Adoptive transfer studies indicate that uterine NK (uNK) cells arise from circulating progenitors. However, virgin uteri contain few circulating NK1.1+CD49a- conventional NK cells, whereas NK1.1+CD49a+ tissue-resident NK (trNK) cells are abundant. In this study, we employed a novel, immune-competent NK cell-specific reporter mouse to track accumulation of uNK cells during unmanipulated pregnancies. We identified conventional NK and trNK cells accumulating in both decidua basalis and myometrium. Only trNK cells showed evidence of proliferation. In parabiosis studies using experimentally induced deciduomata, the accumulated uNK cells were proliferating trNK cells; migrating NK cells made no contribution. Together, these data suggest proliferating trNK cells are the source of uNK cells during endometrial decidualization.


Subject(s)
Cell Movement/immunology , Cell Proliferation/physiology , Decidua/cytology , Killer Cells, Natural/immunology , Pregnancy, Animal , Animals , Antigens, Ly/genetics , Antigens, Ly/metabolism , Decidua/immunology , Female , Green Fluorescent Proteins/genetics , Killer Cells, Natural/cytology , Mice , Mice, Inbred C57BL , Natural Cytotoxicity Triggering Receptor 1/genetics , Natural Cytotoxicity Triggering Receptor 1/metabolism , Parabiosis , Pregnancy
6.
J Infect Dis ; 220(7): 1099-1108, 2019 08 30.
Article in English | MEDLINE | ID: mdl-30715405

ABSTRACT

BACKGROUND: Bacterial vaginosis (BV) is a common imbalance of the vaginal microbiota characterized by overgrowth of diverse Actinobacteria, Firmicutes, and Gram-negative anaerobes. Women with BV are at increased risk of secondary reproductive tract infections and adverse pregnancy outcomes. However, which specific bacteria cause clinical features of BV is unclear. METHODS: We previously demonstrated that Gardnerella vaginalis could elicit many BV features in mice. In this study, we established a BV model in which we coinfected mice with G. vaginalis and another species commonly found in women with BV: Prevotella bivia. RESULTS: This coinfection model recapitulates several aspects of human BV, including vaginal sialidase activity (a diagnostic BV feature independently associated with adverse outcomes), epithelial exfoliation, and ascending infection. It is notable that G. vaginalis facilitated uterine infection by P. bivia. CONCLUSIONS: Taken together, our model provides a framework for advancing our understanding of the role of individual or combinations of BV-associated bacteria in BV pathogenesis.


Subject(s)
Coinfection/microbiology , Gardnerella vaginalis/genetics , Phenotype , Prevotella/genetics , Vaginosis, Bacterial/microbiology , Animals , Disease Models, Animal , Female , Humans , Mice , Mice, Inbred C57BL , Microbiota , Neuraminidase/analysis , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/genetics , Reverse Transcriptase Polymerase Chain Reaction , Vagina/microbiology
7.
Biol Reprod ; 99(5): 922-937, 2018 11 01.
Article in English | MEDLINE | ID: mdl-29733339

ABSTRACT

Preterm birth affects approximately 1 out of every 10 births in the United States, leading to high rates of mortality and long-term negative health consequences. To investigate the mechanisms leading to preterm birth so as to develop prevention strategies, researchers have developed numerous mouse models of preterm birth. However, the lack of standard definitions for preterm birth in mice limits our field's ability to compare models and make inferences about preterm birth in humans. In this review, we discuss numerous mouse preterm birth models, propose guidelines for experiments and reporting, and suggest markers that can be used to assess whether pups are premature or mature. We argue that adoption of these recommendations will enhance the utility of mice as models for preterm birth.


Subject(s)
Obstetric Labor, Premature/physiopathology , Animals , Disease Models, Animal , Female , Humans , Mice , Pregnancy
8.
Semin Immunol ; 26(2): 127-31, 2014 Apr.
Article in English | MEDLINE | ID: mdl-24548893

ABSTRACT

Conventional NK cells are well characterized in the mouse spleen and circulate in the blood. Less well described are NK cells found in organs such as the liver, thymus, and uterus. Recently we identified a tissue-resident NK (trNK) cell population in the liver, suggesting a potential diversity of trNK cells in other organs. In this review we compare and contrast the similarities and differences among the subpopulations of NK and innate lymphoid cells to the trNK cells in the liver.


Subject(s)
Killer Cells, Natural/immunology , Lymphoid Tissue/cytology , Lymphoid Tissue/immunology , Animals , Humans , Killer Cells, Natural/cytology , Killer Cells, Natural/metabolism , Lymphocyte Subsets/cytology , Lymphocyte Subsets/immunology , Lymphocyte Subsets/metabolism , Phenotype
9.
J Immunol ; 195(10): 4973-85, 2015 Nov 15.
Article in English | MEDLINE | ID: mdl-26453755

ABSTRACT

NK cells are innate lymphoid cells important for immune surveillance, identifying and responding to stress, infection, and/or transformation. Whereas conventional NK (cNK) cells circulate systemically, many NK cells reside in tissues where they appear to be poised to locally regulate tissue function. In the present study, we tested the contribution of tissue-resident NK (trNK) cells to tissue homeostasis by studying ischemic injury in the mouse kidney. Parabiosis experiments demonstrate that the kidney contains a significant fraction of trNK cells under homeostatic conditions. Kidney trNK cells developed independent of NFIL3 and T-bet, and they expressed a distinct cell surface phenotype as compared with cNK cells. Among these, trNK cells had reduced asialo-GM1 (AsGM1) expression relative to cNK cells, a phenotype observed in trNK cells across multiple organs and mouse strains. Strikingly, anti-AsGM1 Ab treatment, commonly used as an NK cell-depleting regimen, resulted in a robust and selective depletion of cNKs, leaving trNKs largely intact. Using this differential depletion, we tested the relative contribution of cNK and trNK cells in ischemic kidney injury. Whereas anti-NK1.1 Ab effectively depleted both trNK and cNK cells and protected against ischemic/reperfusion injury, anti-AsGM1 Ab preferentially depleted cNK cells and failed to protect against injury. These data demonstrate unanticipated specificity of anti-AsGM1 Ab depletion on NK cell subsets and reveal a new approach to study the contributions of cNK and trNK cells in vivo. In total, these data demonstrate that trNK cells play a key role in modulating local responses to ischemic tissue injury in the kidney and potentially other organs.


Subject(s)
Antibodies/pharmacology , G(M1) Ganglioside/immunology , Ischemia/immunology , Kidney Diseases/immunology , Kidney/blood supply , Kidney/immunology , Killer Cells, Natural/immunology , Animals , G(M1) Ganglioside/antagonists & inhibitors , Ischemia/pathology , Kidney/pathology , Kidney Diseases/pathology , Killer Cells, Natural/pathology , Mice , Mice, Inbred BALB C , Mice, Inbred NOD , Mice, Knockout
10.
Proc Natl Acad Sci U S A ; 110(1): E50-9, 2013 Jan 02.
Article in English | MEDLINE | ID: mdl-23248310

ABSTRACT

Natural killer (NK) cells provide in vivo control of orthopoxvirus infections in association with their expansion in the draining lymph node (LN), where they are normally very rare. The mechanism of this expansion is unclear. Herein, we determined that NK-cell depletion results in enhanced infection following footpad inoculation of cowpox virus, a natural pathogen of rodents. Following cowpox virus infection in normal mice, NK cells were greatly expanded in the draining LN, were not replicating, and displayed markers similar to splenic NK cells, suggesting specific recruitment of splenic NK cells rather than in situ proliferation. Moreover, NK-cell expansion was abrogated by prior injection of clodronate-loaded liposomes, indicating a role for subcapsular sinus macrophages. Furthermore, recruitment of transferred splenic NK cells to the draining LN was pertussis toxin-sensitive, suggesting involvement of chemokine receptors. Comprehensive analysis of chemokine mRNA expression in the draining LN following infection suggested the selective involvement of CCR2, CCR5, and/or CXCR3. Mice deficient for CCR2 or CCR5 had normal NK-cell recruitment, whereas CXCR3-deficient mice displayed a major defect, which was NK cell-intrinsic. Interestingly, both induction of transcripts for CXCR3 ligands (Cxcl9 and Cxcl10) and NK-cell recruitment required IFN-γ. These data indicate that NK-cell recruitment is mediated by subcapsular sinus macrophages, IFN-γ, and CXCR3 during orthopoxvirus infection.


Subject(s)
Chemokines/immunology , Cowpox virus/immunology , Immunity, Innate/immunology , Interferon-gamma/immunology , Killer Cells, Natural/immunology , Poxviridae Infections/immunology , Adoptive Transfer , Animals , Chlorocebus aethiops , Flow Cytometry , Gene Expression Profiling , Lymph Nodes/cytology , Lymph Nodes/immunology , Macrophages/immunology , Mice , Mice, Inbred C57BL , Receptors, CCR2/metabolism , Receptors, CCR5/metabolism , Vero Cells
11.
J Immunol ; 188(3): 947-51, 2012 Feb 01.
Article in English | MEDLINE | ID: mdl-22227565

ABSTRACT

A key consequence of regulatory T cell (Treg) suppression of CD4 T cells is the inhibition of IL-2 production, yet how Tregs attenuate IL-2 has not been defined. Current models predict a termination of TCR signaling, by disrupting T-APC contacts, or TCR signal modification, through mechanisms such as cAMP. To directly define Treg effects on TCR signaling in CD4 T cell targets, we visualized changes in nuclear accumulation of transcription factors at time points when IL-2 was actively suppressed. Nuclear accumulation of NFAT was highly dependent on sustained TCR signaling in the targets. However, in the presence of Tregs, NFAT and AP-1 signals were sustained in the target cells. In contrast, NF-κB p65 was selectively attenuated. Thus, Tregs do not generally terminate TCR signals. Rather, Tregs selectively modulate TCR signals within hours of contact with CD4 targets, independent of APCs, resulting in the specific loss of NF-κB p65 signals.


Subject(s)
CD4-Positive T-Lymphocytes/metabolism , Cell Nucleus/metabolism , Signal Transduction/immunology , T-Lymphocytes, Regulatory/immunology , Transcription Factor RelA/metabolism , Animals , CD4-Positive T-Lymphocytes/immunology , Interleukin-2/biosynthesis , Mice , NFATC Transcription Factors/metabolism , T-Lymphocytes/metabolism , Transcription Factor AP-1/metabolism , Transcription Factors/metabolism
12.
Proc Natl Acad Sci U S A ; 108(45): 18336-41, 2011 Nov 08.
Article in English | MEDLINE | ID: mdl-22025707

ABSTRACT

CD4(+)CD25(+)Forkhead box P3 (Foxp3)(+) regulatory T cells (Tregs) control immune responses to self and foreign antigens in secondary lymphoid organs and at tissue sites of inflammation. Tregs can modify the function of many immune cells and have been proposed to block early proliferation, differentiation, and effector function. Acute ablation of Tregs has revealed rapid cytokine production immediately after Treg removal, suggesting that Tregs may regulate effector function acutely rather than regulating the programming for immune function. We developed in vitro and in vivo models that enabled the direct test of Treg regulation of T-helper cell type 1 (Th1) differentiation. CD28 signaling is known to abrogate Treg suppression of IL-2 secretion and proliferation, but our studies show that Treg suppression of IFN-γ during Th1 priming proceeds despite enhanced CD28 signaling. Importantly, during Th1 differentiation, Tregs inhibited early IFN-γ transcription without disrupting expression of Th1-specific T-box transcription factor (Tbet) and Th1 programming. Acute shutoff of effector cytokine production by Tregs was selective for IFN-γ but not TNF-α and was independent of TGF-ß and Epstein-Barr virus-induced gene 3. In vivo, Tregs potently controlled CD4 IFN-γ and CD4 effector cell expansion in the lymph node (four- to fivefold reduction) but not Th1 programming, independent of IL-10. Tregs additionally reduced CD4 IFN-γ in the inflamed dermis (twofold reduction) dependent on their production of IL-10. We propose a model for Treg inhibition of effector function based on acute cytokine regulation. Interestingly, Tregs used different regulatory mechanisms to regulate IFN-γ (IL-10-dependent or -independent) subject to the target T-cell stage of activation and its tissue location.


Subject(s)
Cell Differentiation , Interferon-gamma/biosynthesis , Interleukin-10/physiology , T-Lymphocytes, Helper-Inducer/cytology , T-Lymphocytes, Regulatory/immunology , Animals , Cell Compartmentation , Cell Lineage , Flow Cytometry , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL
13.
Blood ; 115(17): 3498-507, 2010 Apr 29.
Article in English | MEDLINE | ID: mdl-20032499

ABSTRACT

Patients with Wiskott-Aldrich syndrome (WAS) have numerous immune cell deficiencies, but it remains unclear how abnormalities in individual cell types contribute to the pathologies of WAS. In T cells, the WAS protein (WASp) regulates actin polymerization and transcription, and plays a role in the dynamics of the immunologic synapse. To examine how these events influence CD4 function, we isolated the WASp deficiency to CD4(+) T cells by adoptive transfer into wild-type mice to study T-cell priming and effector function. WAS(-/-) CD4(+) T cells mediated protective T-helper 1 (Th1) responses to Leishmania major in vivo, but were unable to support Th2 immunity to Nippostrongylus brasiliensis or L major. Mechanistically, WASp was not required for Th2 programming but was required for Th2 effector function. WAS(-/-) CD4(+) T cells up-regulated IL-4 and GATA3 mRNA and secreted IL-4 protein during Th2 differentiation. In contrast, cytokine transcription was uncoupled from protein production in WAS(-/-) Th2-primed effectors. WAS(-/-) Th2s failed to produce IL-4 protein on restimulation despite elevated IL-4/GATA3 mRNA. Moreover, dominant-negative WASp expression in WT effector T cells blocked IL-4 production, but had no effect on IFNgamma. Thus WASp plays a selective, posttranscriptional role in Th2 effector function.


Subject(s)
Th2 Cells/immunology , Wiskott-Aldrich Syndrome Protein/immunology , Animals , GATA3 Transcription Factor/biosynthesis , GATA3 Transcription Factor/genetics , GATA3 Transcription Factor/immunology , Humans , Interferon-gamma/genetics , Interferon-gamma/immunology , Interferon-gamma/metabolism , Interleukin-4/biosynthesis , Interleukin-4/genetics , Interleukin-4/immunology , Leishmania major/immunology , Leishmaniasis, Cutaneous/genetics , Leishmaniasis, Cutaneous/immunology , Leishmaniasis, Cutaneous/metabolism , Mice , Mice, Inbred BALB C , Mice, Knockout , Nippostrongylus/immunology , RNA, Messenger/biosynthesis , RNA, Messenger/genetics , RNA, Messenger/immunology , Strongylida Infections/genetics , Strongylida Infections/immunology , Strongylida Infections/metabolism , Th1 Cells/immunology , Th2 Cells/metabolism , Transcription, Genetic/genetics , Transcription, Genetic/immunology , Up-Regulation/genetics , Up-Regulation/immunology , Wiskott-Aldrich Syndrome/genetics , Wiskott-Aldrich Syndrome/immunology , Wiskott-Aldrich Syndrome/metabolism , Wiskott-Aldrich Syndrome Protein/genetics , Wiskott-Aldrich Syndrome Protein/metabolism
15.
Eur J Immunol ; 39(6): 1544-51, 2009 Jun.
Article in English | MEDLINE | ID: mdl-19462377

ABSTRACT

CTLA-4 is constitutively expressed by CD4(+)CD25(+)Foxp3(+) Treg but its precise role in Treg function is not clear. Although blockade of CTLA-4 interferes with Treg function, studies using CTLA-4-deficient Treg have failed to reveal an essential requirement for CTLA-4 in Treg suppression in vivo. Conditional deletion of CTLA-4 in Foxp3(+) T cells disrupts immune homeostasis in vivo but the immune processes disrupted by CTLA-4 deletion have not been determined. We demonstrate that Treg expression of CTLA-4 is essential for Treg control of lymphopenia-induced CD4 T-cell expansion. Despite IL-10 expression, CTLA-4-deficient Treg were unable to control the expansion of CD4(+) target cells in a lymphopenic environment. Moreover, unlike their WT counterparts, CTLA-4-deficient Treg failed to inhibit cytokine production associated with homeostatic expansion and were unable to prevent colitis. Thus, while Treg developing in the absence of CTLA-4 appear to acquire some compensatory suppressive mechanisms in vitro, we identify a non-redundant role for CTLA-4 in Treg function in vivo.


Subject(s)
Antigens, CD/physiology , CD4-Positive T-Lymphocytes/immunology , Interleukin-2 Receptor alpha Subunit/metabolism , Lymphopenia/immunology , T-Lymphocytes, Regulatory/immunology , T-Lymphocytes, Regulatory/metabolism , Adoptive Transfer , Animals , Antigens, CD/metabolism , Antigens, Surface/metabolism , CD4-Positive T-Lymphocytes/metabolism , CD4-Positive T-Lymphocytes/transplantation , CTLA-4 Antigen , Cell Proliferation/drug effects , Colitis/immunology , Colitis/prevention & control , Cytokines/genetics , Cytokines/metabolism , DNA-Binding Proteins/genetics , Enzyme Inhibitors/pharmacology , Immune Tolerance , Indoleamine-Pyrrole 2,3,-Dioxygenase/antagonists & inhibitors , Lymph Nodes/cytology , Lymph Nodes/immunology , Lymphopenia/genetics , Lymphopenia/pathology , Mice , Mice, Inbred C57BL , Mice, Knockout , Mice, Transgenic , Receptors, Transforming Growth Factor beta/genetics , Spleen/cytology , Spleen/immunology , Tryptophan/analogs & derivatives , Tryptophan/pharmacology , Lymphocyte Activation Gene 3 Protein
16.
Front Immunol ; 11: 290, 2020.
Article in English | MEDLINE | ID: mdl-32153593

ABSTRACT

Natural killer (NK) cells are the most abundant lymphocytes at the maternal-fetal interface. Epidemiological data implicate NK cells in human pregnancy outcomes. Discoveries using mouse NK cells have guided subsequent advances in human NK cell biology. However, it remains challenging to identify mouse and human uterine NK (uNK) cell function(s) because of the dynamic changes in the systemic-endocrinological and local uterine structural microenvironments during pregnancy. This review discusses functional similarities and differences between mouse and human NK cells at the maternal-fetal interface.


Subject(s)
Killer Cells, Natural/immunology , Uterus/immunology , Animals , Female , Maternal-Fetal Exchange , Mice , Models, Animal , Pregnancy
17.
JCI Insight ; 5(5)2020 03 12.
Article in English | MEDLINE | ID: mdl-32161194

ABSTRACT

Herpes simplex virus-2 (HSV-2) and HSV-1 both can cause genital herpes, a chronic infection that establishes a latent reservoir in the nervous system. Clinically, the recurrence frequency of HSV-1 genital herpes is considerably less than HSV-2 genital herpes, which correlates with reduced neuronal infection. The factors dictating the disparate outcomes of HSV-1 and HSV-2 genital herpes are unclear. In this study, we show that vaginal infection of mice with HSV-1 leads to the rapid appearance of mature DCs in the draining lymph node, which is dependent on an early burst of NK cell-mediated IFN-γ production in the vagina that occurs after HSV-1 infection but not HSV-2 infection. Rapid DC maturation after HSV-1 infection, but not HSV-2 infection, correlates with the accelerated generation of a neuroprotective T cell response and early accumulation of IFN-γ-producing T cells at the site of infection. Depletion of T cells or loss of IFN-γ receptor (IFN-γR) expression in sensory neurons both lead to a marked loss of neuroprotection only during HSV-1, recapitulating a prominent feature of HSV-2 infection. Our experiments reveal key differences in host control of neuronal HSV-1 and HSV-2 infection after genital exposure of mice, and they define parameters of a successful immune response against genital herpes.


Subject(s)
Herpes Simplex/immunology , Nervous System Diseases/immunology , Nervous System Diseases/virology , T-Lymphocytes/immunology , Animals , Cell Differentiation , Female , Herpes Simplex/metabolism , Herpes Simplex/virology , Herpesvirus 1, Human/pathogenicity , Herpesvirus 2, Human/pathogenicity , Host-Pathogen Interactions , Interferon-gamma/biosynthesis , Mice , Mice, Inbred C57BL
18.
Front Immunol ; 10: 960, 2019.
Article in English | MEDLINE | ID: mdl-31118936

ABSTRACT

Natural killer (NK) cells are members of a rapidly expanding family of innate lymphoid cells (ILCs). While most previously studied NK cells were derived from the mouse spleen and circulate in the blood, recently others and we found tissue-resident NK (trNK) cells in many tissues that resemble group 1 ILCs (ILC1s). During pregnancy, NK cells are the most abundant lymphocytes in the uterus at the maternal-fetal interface and are involved in placental vascular remodeling. Prior studies suggested that these uterine NK (uNK) cells are mostly derived from circulating NK cells. However, the murine virgin uterus contains mostly trNK cells and it has been challenging to determine their contribution to uNK cells in pregnancy as well as other potential function(s) of uNK cells due to the dynamic microenvironment in the pregnant uterus. This review focuses on the origins and functions of the heterogeneous populations of uNK cells during the course of murine pregnancy.


Subject(s)
Killer Cells, Natural/immunology , Uterus/cytology , Uterus/immunology , Animals , Female , Humans , Mice
19.
J Exp Med ; 216(1): 117-132, 2019 01 07.
Article in English | MEDLINE | ID: mdl-30559127

ABSTRACT

Tissue-resident memory CD8+ T cells (TRMs) confer rapid protection and immunity against viral infections. Many viruses have evolved mechanisms to inhibit MHCI presentation in order to evade CD8+ T cells, suggesting that these mechanisms may also apply to TRM-mediated protection. However, the effects of viral MHCI inhibition on the function and generation of TRMs is unclear. Herein, we demonstrate that viral MHCI inhibition reduces the abundance of CD4+ and CD8+ TRMs, but its effects on the local microenvironment compensate to promote antigen-specific CD8+ TRM formation. Unexpectedly, local cognate antigen enhances CD8+ TRM development even in the context of viral MHCI inhibition and CD8+ T cell evasion, strongly suggesting a role for in situ cross-presentation in local antigen-driven TRM differentiation. However, local cognate antigen is not required for CD8+ TRM maintenance. We also show that viral MHCI inhibition efficiently evades CD8+ TRM effector functions. These findings indicate that viral evasion of MHCI antigen presentation has consequences on the development and response of antiviral TRMs.


Subject(s)
CD4-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/immunology , Immune Evasion , Immunologic Memory , Virus Diseases/immunology , Animals , CD4-Positive T-Lymphocytes/pathology , CD8-Positive T-Lymphocytes/pathology , Chlorocebus aethiops , Dogs , Madin Darby Canine Kidney Cells , Mice , Mice, Transgenic , Vero Cells , Virus Diseases/genetics , Virus Diseases/pathology
20.
Immunology ; 124(1): 13-22, 2008 May.
Article in English | MEDLINE | ID: mdl-18346152

ABSTRACT

Naturally-occurring regulatory T cells (Tregs) are emerging as key regulators of immune responses to self-tissues and infectious agents. Insight has been gained into the cell types and the cellular events that are regulated by Tregs. Indeed, Tregs have been implicated in the control of initial activation events, proliferation, differentiation and effector function. However, the mechanisms by which Tregs disable their cellular targets are not well understood. Here we review recent advances in the identification of distinct mechanisms of Treg action and of signals that enable cellular targets to escape regulation. Roles for inhibitory cytokines, cytotoxic molecules, modulators of cAMP and cytokine competition have all been demonstrated. The growing number of inhibitory mechanisms ascribed to Tregs suggests that Tregs take a multi-pronged approach to immune regulation. It is likely that the relative importance of each inhibitory mechanism is context dependent and modulated by the inflammatory milieu and the magnitude of the immune response. In addition, the target cell may be differentially susceptible or resistant to distinct Treg mechanisms depending on their activation or functional status at the time of the Treg encounter. Understanding when and where each suppressive tool is most effective will help to fine tune therapeutic strategies to promote or constrain specific arms of Treg suppression.


Subject(s)
Immune Tolerance/immunology , T-Lymphocytes, Regulatory/immunology , Animals , Binding, Competitive , Cell Communication/immunology , Cytokines/metabolism , Growth Substances/metabolism
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